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Increase of secondary metabolites in sweet basil (Ocimum basilicum L.) leaves by exposure to N 2 O 5 with plasma technology.
- Source :
-
Scientific reports [Sci Rep] 2024 Jun 04; Vol. 14 (1), pp. 12759. Date of Electronic Publication: 2024 Jun 04. - Publication Year :
- 2024
-
Abstract
- Exposure to N <subscript>2</subscript> O <subscript>5</subscript> generated by plasma technology activates immunity in Arabidopsis through tryptophan metabolites. However, little is known about the effects of N <subscript>2</subscript> O <subscript>5</subscript> exposure on other plant species. Sweet basil synthesizes many valuable secondary metabolites in its leaves. Therefore, metabolomic analyses were performed at three different exposure levels [9.7 (Ex1), 19.4 (Ex2) and 29.1 (Ex3) μmol] to assess the effects of N <subscript>2</subscript> O <subscript>5</subscript> on basil leaves. As a result, cinnamaldehyde and phenolic acids increased with increasing doses. Certain flavonoids, columbianetin, and caryophyllene oxide increased with lower Ex1 exposure, cineole and methyl eugenol increased with moderate Ex2 exposure and L-glutathione GSH also increased with higher Ex3 exposure. Furthermore, gene expression analysis by quantitative RT-PCR showed that certain genes involved in the syntheses of secondary metabolites and jasmonic acid were significantly up-regulated early after N <subscript>2</subscript> O <subscript>5</subscript> exposure. These results suggest that N <subscript>2</subscript> O <subscript>5</subscript> exposure increases several valuable secondary metabolites in sweet basil leaves via plant defense responses in a controllable system.<br /> (© 2024. The Author(s).)
- Subjects :
- Gene Expression Regulation, Plant
Metabolomics methods
Flavonoids metabolism
Eugenol analogs & derivatives
Eugenol metabolism
Oxylipins metabolism
Ocimum basilicum metabolism
Ocimum basilicum genetics
Plant Leaves metabolism
Plant Leaves drug effects
Plant Leaves genetics
Secondary Metabolism drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 38834771
- Full Text :
- https://doi.org/10.1038/s41598-024-63508-8