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EPA and DHA differentially improve insulin resistance by reducing adipose tissue inflammation-targeting GPR120/PPARγ pathway.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2024 Aug; Vol. 130, pp. 109648. Date of Electronic Publication: 2024 Apr 15. - Publication Year :
- 2024
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Abstract
- Insulin resistance (IR) is a global health challenge, often initiated by dysfunctional adipose tissue. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) may have different effects on IR, but the mechanisms are unknown. This study aims to evaluate the protective effect of EPA and DHA against IR in a high-fat diet (HFD) mice model and investigate whether EPA and DHA alter IR modulate the G-protein-poupled receptor 120/peroxisome proliferator-activated receptor γ (GPR120/PPARγ) pathway in macrophages and adipocytes, which may affect IR in adipocytes. The findings of this study show that 4% DHA had a better effect in improving IR and reducing inflammatory cytokines in adipose tissue of mice. Additionally, in the cell experiment, the use of AH7614 (a GPR120 antagonist) inhibited the glucose consumption increase and the increasable expression of PPARγ and insulin signaling molecules mediated by DHA in adipocytes. Furthermore, GW9662 (a PPARγ antagonist) hindered the upregulation of glucose consumption and insulin signaling molecule expression induced by EPA and DHA in adipocytes. DHA exhibited significant effects in reducing the number of migrated cells and inflammation. The compounds AH7614 and GW9662 hindered the suppressive effects of EPA and DHA on macrophage-induced IR in adipocytes. These findings suggest that DHA has a stronger potential in improving IR in adipocytes through the GPR120/PPARγ pathway in macrophages, when compared to EPA.<br /> (Copyright © 2024. Published by Elsevier Inc.)
- Subjects :
- Animals
Mice
Male
Adipocytes drug effects
Adipocytes metabolism
3T3-L1 Cells
Macrophages metabolism
Macrophages drug effects
RAW 264.7 Cells
Anilides pharmacology
Biphenyl Compounds
Phenylpropionates
Docosahexaenoic Acids pharmacology
PPAR gamma metabolism
Insulin Resistance
Eicosapentaenoic Acid pharmacology
Receptors, G-Protein-Coupled metabolism
Adipose Tissue metabolism
Adipose Tissue drug effects
Mice, Inbred C57BL
Diet, High-Fat adverse effects
Inflammation metabolism
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4847
- Volume :
- 130
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38631512
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2024.109648