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Dioscin Attenuates Interleukin 1β (IL-1β)-Induced Catabolism and Apoptosis via Modulating the Toll-Like Receptor 4 (TLR4)/Nuclear Factor kappa B (NF-κB) Signaling in Human Nucleus Pulposus Cells.
- Source :
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Medical science monitor : international medical journal of experimental and clinical research [Med Sci Monit] 2020 Aug 25; Vol. 26, pp. e923386. Date of Electronic Publication: 2020 Aug 25. - Publication Year :
- 2020
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Abstract
- BACKGROUND Nucleus pulposus (NP) cell dysfunction and apoptosis contribute to disc degeneration. Dioscin, a natural steroid saponin, has been demonstrated to have anti-inflammatory, antiapoptotic, and antioxidative effects in various diseases. However, little is known about the roles of dioscin in intervertebral disc degeneration. MATERIAL AND METHODS To evaluate the roles of dioscin in disc degeneration and its specific mechanism, human NP cells were incubated with IL-1ß and various concentrations of dioscin. Cell viability, extracellular matrix protein expression, catabolic factors, degree of apoptosis, inflammatory factors, and related signaling pathways were evaluated by western blotting, fluorescence immunostaining, TUNEL staining, and reverse transcription PCR. RESULTS Dioscin inhibited IL-1ß-activated apoptotic signaling and catabolic activity in NP cells. Dioscin suppressed TLR4/NF-0kappaB signaling, and attenuated the level of inflammatory mediators (IL-6, TNF-alpha) in IL-1ß-stimulated human NP cells. CONCLUSIONS Our work provides the first evidence that dioscin attenuates IL-1ß-activated inflammation and catabolic activity in human NP cells through inhibiting the TLR4/NF-kappaB pathway, indicating that dioscin is a new potential candidate for clinical therapy to attenuate disc degeneration.
- Subjects :
- Diosgenin pharmacology
Humans
Inflammation Mediators metabolism
NF-kappa B metabolism
Nucleus Pulposus cytology
Nucleus Pulposus metabolism
Toll-Like Receptor 4 metabolism
Tumor Necrosis Factor-alpha metabolism
Apoptosis drug effects
Diosgenin analogs & derivatives
Interleukin-1beta metabolism
NF-kappa B drug effects
Nucleus Pulposus drug effects
Signal Transduction drug effects
Toll-Like Receptor 4 drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1643-3750
- Volume :
- 26
- Database :
- MEDLINE
- Journal :
- Medical science monitor : international medical journal of experimental and clinical research
- Publication Type :
- Academic Journal
- Accession number :
- 32841225
- Full Text :
- https://doi.org/10.12659/MSM.923386