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Evidence of Campylobacter jejuni reduction in broilers with early synbiotic administration.
- Source :
-
International journal of food microbiology [Int J Food Microbiol] 2017 Jun 19; Vol. 251, pp. 41-47. Date of Electronic Publication: 2017 Apr 03. - Publication Year :
- 2017
-
Abstract
- C. jejuni is considered a food safety concern to both public health authorities and consumers since it is the leading bacterial cause of food-borne gastroenteritis in humans. A high incidence of C. jejuni in broiler flocks is often correlated to pathogen recovery in retail poultry meat, which is the main source of human infection. In this work broiler chickens were fed with a synbiotic product mixed with conventional feed using two different administration strategies. The synbiotic was formulated with the microencapsulated probiotic Bifidobacterium longum PCB133 and a xylo-oligosaccharide (XOS). 1-day old chicks were infected with C. jejuni strain M1 (10 <superscript>5</superscript> cells) and the synbiotic mixture was then administered starting from the first and the 14th day of chicken life (for animal groups GrpC and GrpB respectively). The goal of this study was to monitor C. jejuni load at caecum level at different sampling time by real-time PCR, identifying the best administration strategy. The microbiological analysis of the caecal content also considered the quantification of Campylobacter spp., Bifidobacterium spp. and B. longum. The supplemented synbiotic was more successful in reducing C. jejuni and Campylobacter spp. when administered lifelong, compared to the shorter supplementation (GrpB). Bifidobacterium spp. quantification did not show significant differences among treatments and B. longum PCB133 was detected in both supplemented groups evidencing the successful colonization of the strain. Moreover, the samples of the control group (GrpA) and GrpC were analysed with PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to compare the caecal microbial community profiles at the beginning and at the end of the trial. Pattern analysis evidenced the strong influence of the early synbiotic supplementation, although a physiological change in the microbial community, occurring during growth, could be observed. Experimental results demonstrate that the synbiotic approach at farm level can be an effective strategy, combined with biosecurity measures, to improve the safety of poultry meat.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bifidobacterium genetics
Campylobacter Infections microbiology
Campylobacter Infections veterinary
Campylobacter jejuni genetics
DNA, Bacterial genetics
Foodborne Diseases microbiology
Humans
Molecular Typing
Poultry microbiology
Poultry Diseases microbiology
Poultry Diseases prevention & control
Real-Time Polymerase Chain Reaction
Synbiotics
Bifidobacterium isolation & purification
Biological Control Agents administration & dosage
Campylobacter jejuni isolation & purification
Cecum microbiology
Chickens microbiology
Foodborne Diseases prevention & control
Glucuronates pharmacology
Oligosaccharides pharmacology
Probiotics therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3460
- Volume :
- 251
- Database :
- MEDLINE
- Journal :
- International journal of food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 28390936
- Full Text :
- https://doi.org/10.1016/j.ijfoodmicro.2017.04.001