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Ex vivo digestion of carp muscle tissue--ACE inhibitory and antioxidant activities of the obtained hydrolysates.
- Source :
-
Food & function [Food Funct] 2015 Jan; Vol. 6 (1), pp. 211-8. Date of Electronic Publication: 2014 Nov 04. - Publication Year :
- 2015
-
Abstract
- In the digestive tract of humans, bioactive peptides, i.e. protein fragments impacting the physiological activity of the body, may be released during the digestion of food proteins, including those of fish. The aim of the study was to establish the method of human ex vivo digestion of carp muscle tissue and evaluate the angiotensin I-converting enzyme inhibitory and antioxidant activities of hydrolysates obtained after digestion. It was found that the hydrolysates of carp muscle tissue obtained with the three-stage method of simulated ex vivo digestion showed ACE inhibitory as well as antioxidative activities. It was demonstrated that the degree of hydrolysis depended on the duration of individual stages and the degree of comminution of the examined material. Although the applied gastric juices initiated the process of hydrolysis of carp muscle tissue, the duodenal juices caused a rapid increase in the amount of hydrolysed polypeptide bonds. The antihypertensive and antioxidative activities of the hydrolysates of carp muscle tissue increased together with progressive protein degradation. However, the high degree of protein hydrolysis does not favour an increase in the activity of free radical scavenging. The presented results are an example of the first preliminary screening of the potential health-promoting biological activity of carp muscle tissue in an ex vivo study.
- Subjects :
- Angiotensin-Converting Enzyme Inhibitors analysis
Angiotensin-Converting Enzyme Inhibitors chemistry
Angiotensin-Converting Enzyme Inhibitors pharmacology
Animals
Antihypertensive Agents analysis
Antihypertensive Agents chemistry
Antihypertensive Agents metabolism
Antihypertensive Agents pharmacology
Antioxidants analysis
Antioxidants chemistry
Antioxidants pharmacology
Aquaculture
Dietary Proteins analysis
Dietary Proteins chemistry
Dietary Proteins metabolism
Dietary Proteins pharmacology
Duodenum
Fish Proteins analysis
Fish Proteins chemistry
Fish Proteins metabolism
Fish Proteins pharmacology
Gastric Juice chemistry
Gastric Juice enzymology
Gastric Juice metabolism
Humans
Intestinal Secretions chemistry
Intestinal Secretions enzymology
Intestinal Secretions metabolism
Kinetics
Muscle Proteins analysis
Muscle Proteins chemistry
Muscle Proteins metabolism
Muscle Proteins pharmacology
Muscle, Skeletal chemistry
Oligopeptides analysis
Oligopeptides chemistry
Oligopeptides metabolism
Oligopeptides pharmacology
Peptide Fragments analysis
Peptide Fragments chemistry
Peptide Fragments metabolism
Peptide Fragments pharmacology
Poland
Protein Hydrolysates chemistry
Protein Hydrolysates metabolism
Protein Hydrolysates pharmacology
Angiotensin-Converting Enzyme Inhibitors metabolism
Antioxidants metabolism
Carps
Digestion
Functional Food analysis
Models, Biological
Seafood analysis
Subjects
Details
- Language :
- English
- ISSN :
- 2042-650X
- Volume :
- 6
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Food & function
- Publication Type :
- Academic Journal
- Accession number :
- 25367247
- Full Text :
- https://doi.org/10.1039/c4fo00621f