Back to Search
Start Over
Improving flavonoid extraction from Ginkgo biloba leaves by prefermentation processing.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2013 Jun 19; Vol. 61 (24), pp. 5783-91. Date of Electronic Publication: 2013 Jun 06. - Publication Year :
- 2013
-
Abstract
- This paper presents a prefermentation treatment method involving fungi to improve flavonoid extraction from the leaves of Ginkgo biloba . The fungi employed for this treatment were screened from the soil present under an ancient ginkgo tree. Seventy-six strains belonging to 23 genera were isolated and identified by a molecular identification method employing 18S rDNA sequences. Thirty-three strains grew well using ginkgo leaves as the growth medium. One strain, Gyx086, with higher extracted yield of flavonoids and more similar to the control, was finally selected for prefermentation processing. The major fermentation factors were optimized by response surface methodology. The optimal conditions for the highest total falvonoid yield were 27.8 °C for temperature, 64.2% for moisture content, and 61 h for fermentation time. Under the optimal condition, a actual total flavonoid yield of 27.59 ± 0.52 mg/g dry weight culture sample was obtained, which was about 70% higher than that of unfermented gingko leaf samples.
- Subjects :
- Antioxidants analysis
China
Feasibility Studies
Fermentation
Flavones analysis
Fungi classification
Fungi growth & development
Fungi isolation & purification
Ginkgo biloba growth & development
Ginkgo biloba microbiology
Hydrolysis
Lignin analysis
Molecular Typing
Mycological Typing Techniques
Plant Leaves growth & development
Plant Leaves microbiology
Soil Microbiology
Species Specificity
Antioxidants isolation & purification
Dietary Supplements analysis
Flavones isolation & purification
Fungi metabolism
Ginkgo biloba chemistry
Lignin metabolism
Plant Leaves chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 61
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 23713789
- Full Text :
- https://doi.org/10.1021/jf400712n