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Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication.
- Source :
-
Meat science [Meat Sci] 2012 Mar; Vol. 90 (3), pp. 686-9. Date of Electronic Publication: 2011 Oct 31. - Publication Year :
- 2012
-
Abstract
- A commercially available real-time PCR, based on a multi-copy target cytochrome b (cyt b) using porcine specific primers, has been validated for the Halal/Kosher authentication of gelatine. Extraction and purification of DNA from gelatine were successfully achieved using the SureFood® PREP Animal system, and real-time PCR was carried out using SureFood® Animal ID Pork Sens kit. The minimum level of adulteration that could be detected was 1.0% w/w for marshmallows and gum drops. A small survey was undertaken of processed food products such as gum drops, marshmallows and Turkish delight, believed to contain gelatine. Of fourteen food products from Germany, two samples were found to contain porcine gelatine, whereas of twenty-nine samples from Turkey twenty-eight were negative. However, one product from Turkey contained porcine DNA and thus was not Halal, and neither was the use of porcine gelatine indicated on the product label.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cytochromes b genetics
DNA genetics
DNA Primers genetics
Gelatin genetics
Germany
Polymerase Chain Reaction methods
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity
Sequence Analysis, DNA
Turkey
DNA isolation & purification
Food Analysis methods
Food Handling methods
Meat analysis
Swine genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4138
- Volume :
- 90
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Meat science
- Publication Type :
- Academic Journal
- Accession number :
- 22098822
- Full Text :
- https://doi.org/10.1016/j.meatsci.2011.10.014