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Prebiotic properties of alternansucrase maltose-acceptor oligosaccharides.

Authors :
Sanz ML
Côté GL
Gibson GR
Rastall RA
Source :
Journal of agricultural and food chemistry [J Agric Food Chem] 2005 Jul 27; Vol. 53 (15), pp. 5911-6.
Publication Year :
2005

Abstract

alpha-(1-6) and alpha-(1-3)-linked oligosaccharides were obtained from the reaction between sucrose and maltose, catalyzed by an alternansucrase isolated from Leuconostoc mesenteroides NRRL B-21297 and separated using a Bio-Gel P2 column in six fractions. Fractions 1, 2, and 3 were mainly composed of DP3, DP4, and DP5, respectively. However, fractions 4, 5, and 6 consisted of mixtures from DP5 to DP9, and they are identified here as DP5.7, DP6.7, and DP7.4, respectively. Potential prebiotic properties of these oligosaccharides were tested using pure and mixed cultures. Generally, in pure studies, most of the tested bacteria failed to grow or grew poorly using the DP6.7 and DP7.4 fractions and showed the greatest growth on DP3. Growth of fecal bacteria on the maltose-acceptor products was tested following an in vitro fermentation method. DP3 showed the highest prebiotic effect, followed by DP4 and DP6.7, whereas DP7.4 did not present any prebiotic activity.

Details

Language :
English
ISSN :
0021-8561
Volume :
53
Issue :
15
Database :
MEDLINE
Journal :
Journal of agricultural and food chemistry
Publication Type :
Academic Journal
Accession number :
16028973
Full Text :
https://doi.org/10.1021/jf050344e