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Development of a microwave-assisted-extraction-based method for the determination of aflatoxins B, G, B, and G in grains and grain products.
- Source :
-
Analytical & Bioanalytical Chemistry . Feb2013, Vol. 405 Issue 5, p1623-1630. 8p. - Publication Year :
- 2013
-
Abstract
- This article describes the use of microwave-assisted extraction (MAE) as a pretreatment technique for the determination of aflatoxins B, G, B, and G in grains and grain products. The optimal operation parameters, including extraction solvent, temperature, and time, were identified to be acetonitrile as the extraction solvent at 80 °C with 15 min of MAE. The extracts were cleaned up using solid-phase extraction followed by derivatization with trifluoroacetic acid and were determined by liquid chromatography-fluorescence detection. A Sep-Pak cartridge was chosen over Oasis HLB and Bond Elut cartridges. By the use of aflatoxin M as an internal standard, relative recoveries of the aflatoxins ranged from 90.7 to 105.7 % for corn and from 88.1 to 103.4 % for wheat, with relative standard deviations between 2.5 and 8.7 %. A total of 36 samples from local markets were analyzed, and aflatoxin B was found to be the predominant toxin, with concentrations ranging from 0.42 to 3.41 μg/kg. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
- Subjects :
- *AFLATOXINS
*MYCOTOXINS
*ACETONITRILE
*TRIFLUOROACETIC acid
*GRAIN
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 405
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 85013925
- Full Text :
- https://doi.org/10.1007/s00216-012-6564-8