Lipidomics reveals the changes in lipid profile of flaxseed oil affected by roasting.

• The change in lipid profile of flaxseed oil during roasting was studied. • A total of 238 lipids were identified in unroasted and roasted flaxseed oil. • Roasting had an obvious effect on lipid profile of flaxseed oil. • Cyclic phosphatidic acid was identified in flaxseed oil for the first time. • A possible mechanism of degradation and oxidation of flaxseed oil was proposed. Roasting before oil extraction improves the oxidative stability and odor of flaxseed oil; however, the effect of roasting on lipid profile is still unclear. Herein, the changes in lipid profile in flaxseed oil during roasting were investigated based on lipidomic approach. 238 lipids including fatty acid (45 species), phospholipid (37 species), triacylglycerol (125 species), and oxidized fatty acid (21 species) were determined in unroasted and roasted flaxseed oils. After roasting, unsaturated fatty acids including oleic, linoleic, and lenolenic acid decreased. Triacylglycerols such as TAG(18:3/18:3/18:3) and TAG(18:2/18:3/18:3) had the same change trends with unsaturated fatty acids. However, phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols, lysophosphatidylcholines, lysophosphatidylethanolamines, and oxidized fatty acids firstly increased and then decreased during roasting. Cyclic phosphadic acids identified for the first time in flaxseed oils increased rapidly at high-temperature roasting. 23 lipids were determined as potential biomarkers to differentiate the light and dark roasted flaxseed oils. Our finding could provide useful information for flaxseed oil processing and lipidomics. [ABSTRACT FROM AUTHOR]