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Bacillus thermoamylovorans-Related Strain Isolated from High Temperature Sites as Potential Producers of Medium-Chain-Length Polyhydroxyalkanoate (mcl-PHA).

Bacillus thermoamylovorans-Related Strain Isolated from High Temperature Sites as Potential Producers of Medium-Chain-Length Polyhydroxyalkanoate (mcl-PHA).

Authors :
Choonut, Aophat
Prasertsan, Poonsuk
Klomklao, Sappasith
Sangkharak, Kanokphorn
Source :
Current Microbiology. Oct2020, Vol. 77 Issue 10, p3044-3056. 13p.
Publication Year :
2020

Abstract

Thermotolerant bacteria producing medium-chain-length polyhydroxyalkanoate (mcl-PHA) were isolated from various thermal sites, including palm oil mill effluent, textile wastewater, and hot spring water, in Thailand. Fifteen strains were isolated at 45 °C using nutrient-rich (NR) medium. However, only six isolates produced mcl-PHA at 0.41 ± 0.01 g/L to 0.80 ± 0.01 g/L, representing a mcl-PHA content of 29.44% to 50.77% of the dry cell weight (DCW). The six strains of bacterial isolates could utilise a variety of substrates; all were identified as Bacillus thermoamylovorans. The highest mcl-PHA content (50.77% of the DCW) was accumulated by the B. thermoamylovorans strain PHA005 isolated from palm oil mill effluent. The mcl-PHA from strain PHA005 was composed of five different monomers, 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxytetradecanoate (3HTD), 3-hydroxyhexadecanoic acid (3HHD), and 3-hydroxyoctadecanoic (3HOD), with a monomer content of 24.12, 15.50, 13.00, 39.25, and 8.13 mol%, respectively. The optimum temperature for B. thermoamylovorans strain PHA005 growth is 45 °C, and it can survive at up to 60 °C. This is a first report of PHA synthesis by a thermotolerant B. thermoamylovorans. Moreover, the high content of 3HHD monomers (39.25 mol%) has never been reported in Bacillus. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03438651
Volume :
77
Issue :
10
Database :
Academic Search Index
Journal :
Current Microbiology
Publication Type :
Academic Journal
Accession number :
145348210
Full Text :
https://doi.org/10.1007/s00284-020-02118-9