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Isolation and characterization of a gamma-aminobutyric acid producing strain Lactobacillus buchneri WPZ001 that could efficiently utilize xylose and corncob hydrolysate.
- Source :
-
Applied Microbiology & Biotechnology . Apr2015, Vol. 99 Issue 7, p3191-3200. 10p. - Publication Year :
- 2015
-
Abstract
- Lactobacillus buchneri strain WPZ001 that could efficiently produce gamma-aminobutyric acid was isolated from Chinese fermented sausages. Optimal cultivation conditions for gamma-aminobutyric acid production in L. buchneri WPZ001 were determined, and xylose was found to be the best carbon source. Using xylose as the sole carbon source, 70 g/L gamma-aminobutyric acid was produced by flask fermentation of L. buchneri WPZ001 for 48 h, and the harvested cells could continue to convert monosodium glutamate to gamma-aminobutyric acid in buffer and produce 59 g gamma-aminobutyric acid after eight runs of biotransformation; the total yield of gamma-aminobutyric acid reached 129 g/L. This combination strategy also worked well when the low-cost corncob hydrolysate was used as the sole carbon source, and the yield of gamma-aminobutyric acid reached 117 g/L. The results indicate that L. buchneri WPZ001 has great potential for industrial production of gamma-aminobutyric acid. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GABA
*XYLOSE
*CORNCOBS
*LACTOBACILLUS
*HYDROLYSIS
*SAUSAGES
Subjects
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 99
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 101622824
- Full Text :
- https://doi.org/10.1007/s00253-014-6294-2