1. MMP9 modulates the metastatic cascade and immune landscape for breast cancer anti-metastatic therapy.
- Author
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Owyong M, Chou J, van den Bijgaart RJ, Kong N, Efe G, Maynard C, Talmi-Frank D, Solomonov I, Koopman C, Hadler-Olsen E, Headley M, Lin C, Wang CY, Sagi I, Werb Z, and Plaks V
- Subjects
- Animals, Antibodies immunology, Antibodies pharmacology, Breast Neoplasms immunology, Breast Neoplasms pathology, CD8-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes pathology, Carcinogenesis, Cell Line, Tumor, Female, HEK293 Cells, Humans, Lung Neoplasms pathology, Lung Neoplasms secondary, Mammary Neoplasms, Experimental immunology, Mammary Neoplasms, Experimental pathology, Matrix Metalloproteinase 9 immunology, Matrix Metalloproteinase Inhibitors pharmacology, Mice, Mice, Inbred Strains, Neoplasm Invasiveness, Neoplasm Metastasis, Breast Neoplasms enzymology, Mammary Neoplasms, Experimental enzymology, Matrix Metalloproteinase 9 metabolism
- Abstract
Metastasis, the main cause of cancer-related death, has traditionally been viewed as a late-occurring process during cancer progression. Using the MMTV-PyMT luminal B breast cancer model, we demonstrate that the lung metastatic niche is established early during tumorigenesis. We found that matrix metalloproteinase 9 (MMP9) is an important component of the metastatic niche early in tumorigenesis and promotes circulating tumor cells to colonize the lungs. Blocking active MMP9, using a monoclonal antibody specific to the active form of gelatinases, inhibited endogenous and experimental lung metastases in the MMTV-PyMT model. Mechanistically, inhibiting MMP9 attenuated migration, invasion, and colony formation and promoted CD8
+ T cell infiltration and activation. Interestingly, primary tumor burden was unaffected, suggesting that inhibiting active MMP9 is primarily effective during the early metastatic cascade. These findings suggest that the early metastatic circuit can be disrupted by inhibiting active MMP9 and warrant further studies of MMP9-targeted anti-metastatic breast cancer therapy., (© 2019 Owyong et al.)- Published
- 2019
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