Search

Your search keyword '"van Eldik, Mandy"' showing total 4 results
Start Over Author "van Eldik, Mandy"
4 results on '"van Eldik, Mandy"'

2. An improved respiratory syncytial virus neutralization assay based on the detection of green fluorescent protein expression and automated plaque counting

Author

van Remmerden Yvonne, Xu Fang, van Eldik Mandy, Heldens Jacco GM, Huisman Willem, and Widjojoatmodjo Myra N

Subjects
RSV, Virus neutralization, EGFP, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Virus neutralizing antibodies against respiratory syncytial virus (RSV) are considered important correlates of protection for vaccine evaluation. The established plaque reduction assay is time consuming, labor intensive and highly variable. Methods Here, a neutralization assay based on a modified RSV strain expressing the green fluorescent protein in combination with automated detection and quantification of plaques is described. Results The fluorescence plaque reduction assay in microplate format requires only two days to complete and is simple and reproducible. A good correlation between visual and automated counting methods to determine RSV neutralizing serum antibody titers was observed. Conclusions The developed virus neutralization assay is suitable for high-throughput testing and can be used for both animal studies and (large scale) vaccine clinical trials.

Published
2012
Full Text
View/download PDF

3. Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides

Author

van Dijk, Albert, van Eldik, Mandy, Veldhuizen, Edwin J A, Tjeerdsma-van Bokhoven, Hanne L M, de Zoete, Marcel R, Bikker, Floris J, Haagsman, Henk P, Infection & Immunity, dI&I I&I-3, LS Moleculaire Afweer, LS Infectiebiologie (Bacteriologie), Dep Infectieziekten Immunologie, dI&I I&I-2, Orale Biochemie (OII, ACTA), Infection & Immunity, dI&I I&I-3, LS Moleculaire Afweer, LS Infectiebiologie (Bacteriologie), Dep Infectieziekten Immunologie, dI&I I&I-2, and Oral Biochemistry

Subjects
0301 basic medicine, Lipopolysaccharides, Models, Molecular, Chemokine, Transcription, Genetic, Physiology, Protein Conformation, medicine.medical_treatment, Anti-Inflammatory Agents, lcsh:Medicine, Biochemistry, Poultry, Cathelicidin, 0302 clinical medicine, Immune Physiology, Medicine and Health Sciences, Gamefowl, Chemical Neutralization, Tyrosine, Amino Acids, lcsh:Science, Peptide sequence, Innate Immune System, Multidisciplinary, biology, Organic Compounds, Chemotaxis, Chemical Reactions, Neurochemistry, Agriculture, Lipids, Anti-Bacterial Agents, Cell Motility, Chemistry, Cytokine, Physical Sciences, Vertebrates, Cytokines, Neurochemicals, Chemokines, Inflammation Mediators, Research Article, Livestock, Antimicrobial peptides, Immunology, Microbial Sensitivity Tests, Nitric Oxide, Cell Line, Birds, 03 medical and health sciences, Immune system, SDG 3 - Good Health and Well-being, medicine, Escherichia coli, Animals, Immunologic Factors, Amino Acid Sequence, L-Lactate Dehydrogenase, Macrophages, lcsh:R, Organic Chemistry, Chemical Compounds, Organisms, Biology and Life Sciences, Proteins, Cell Biology, Molecular Development, Peptide Fragments, 030104 developmental biology, Amino Acid Substitution, Gene Expression Regulation, Cell culture, Fowl, Immune System, Mutation, biology.protein, lcsh:Q, Reactive Oxygen Species, Chickens, 030215 immunology, Neuroscience, Developmental Biology, Antimicrobial Cationic Peptides
Abstract

Host Defence Peptides and derived peptides are promising classes of antimicrobial and immunomodulatory lead compounds. For this purpose we examined whether chicken cathelicidin-2 (CATH-2)-derived peptides modulate the function and inflammatory response of avian immune cells. Using a chicken macrophage cell line (HD11) we found that full-length CATH-2 dose-dependently induced transcription of chemokines CXCLi2/IL-8, MCP-3 and CCLi4/RANTES, but not of pro-inflammatory cytokine IL-1β. In addition, CATH-2 efficiently inhibited IL-1β and nitric oxide production by HD11 cells induced by different sources of lipopolysaccharides (LPS). N-terminal truncated CATH-2 derived peptides maintained the capacity to selectively induce chemokine transcription, but despite their high LPS affinity several analogs lacked LPS-neutralizing capacity. Substitution of phenylalanine residues by tryptophan introduced endotoxin neutralization capacity in inactive truncated CATH-2 derived peptides. In contrast, amino acid substitution of phenylalanine by tyrosine abrogated endotoxin neutralization activity of CATH-2 analogs. These findings support a pivotal role for aromatic residues in peptide-mediated endotoxin neutralization by CATH-2 analogs and were shown to be independent of LPS affinity. The capacity to modulate chemokine production and dampen endotoxin-induced pro-inflammatory responses in chicken immune cells implicates that small CATH-2 based peptides could serve as leads for the design of CATH-2 based immunomodulatory anti-infectives.

Published
2016

4. Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides

Author

Infection & Immunity, dI&I I&I-3, LS Moleculaire Afweer, LS Infectiebiologie (Bacteriologie), Dep Infectieziekten Immunologie, dI&I I&I-2, van Dijk, Albert, van Eldik, Mandy, Veldhuizen, Edwin J A, Tjeerdsma-van Bokhoven, Hanne L M, de Zoete, Marcel R, Bikker, Floris J, Haagsman, Henk P, Infection & Immunity, dI&I I&I-3, LS Moleculaire Afweer, LS Infectiebiologie (Bacteriologie), Dep Infectieziekten Immunologie, dI&I I&I-2, van Dijk, Albert, van Eldik, Mandy, Veldhuizen, Edwin J A, Tjeerdsma-van Bokhoven, Hanne L M, de Zoete, Marcel R, Bikker, Floris J, and Haagsman, Henk P

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results