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Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 2, p e0147919 (2016), van Dijk, A, van Eldik, M, Veldhuizen, E J A, Tjeerdsma-van Bokhoven, H L M, de Zoete, M R, Bikker, F J & Haagsman, H P 2016, ' Immunomodulatory and anti-inflammatory activities of chicken cathelicidin-2 derived peptides ', PLoS ONE, vol. 11, no. 2, e0147919 . https://doi.org/10.1371/journal.pone.0147919, PLoS ONE, 11(2):e0147919. Public Library of Science, PLoS One, 11(2). Public Library of Science
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- Host Defence Peptides and derived peptides are promising classes of antimicrobial and immunomodulatory lead compounds. For this purpose we examined whether chicken cathelicidin-2 (CATH-2)-derived peptides modulate the function and inflammatory response of avian immune cells. Using a chicken macrophage cell line (HD11) we found that full-length CATH-2 dose-dependently induced transcription of chemokines CXCLi2/IL-8, MCP-3 and CCLi4/RANTES, but not of pro-inflammatory cytokine IL-1β. In addition, CATH-2 efficiently inhibited IL-1β and nitric oxide production by HD11 cells induced by different sources of lipopolysaccharides (LPS). N-terminal truncated CATH-2 derived peptides maintained the capacity to selectively induce chemokine transcription, but despite their high LPS affinity several analogs lacked LPS-neutralizing capacity. Substitution of phenylalanine residues by tryptophan introduced endotoxin neutralization capacity in inactive truncated CATH-2 derived peptides. In contrast, amino acid substitution of phenylalanine by tyrosine abrogated endotoxin neutralization activity of CATH-2 analogs. These findings support a pivotal role for aromatic residues in peptide-mediated endotoxin neutralization by CATH-2 analogs and were shown to be independent of LPS affinity. The capacity to modulate chemokine production and dampen endotoxin-induced pro-inflammatory responses in chicken immune cells implicates that small CATH-2 based peptides could serve as leads for the design of CATH-2 based immunomodulatory anti-infectives.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Models, Molecular
Chemokine
Transcription, Genetic
Physiology
Protein Conformation
medicine.medical_treatment
Anti-Inflammatory Agents
lcsh:Medicine
Biochemistry
Poultry
Cathelicidin
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Gamefowl
Chemical Neutralization
Tyrosine
Amino Acids
lcsh:Science
Peptide sequence
Innate Immune System
Multidisciplinary
biology
Organic Compounds
Chemotaxis
Chemical Reactions
Neurochemistry
Agriculture
Lipids
Anti-Bacterial Agents
Cell Motility
Chemistry
Cytokine
Physical Sciences
Vertebrates
Cytokines
Neurochemicals
Chemokines
Inflammation Mediators
Research Article
Livestock
Antimicrobial peptides
Immunology
Microbial Sensitivity Tests
Nitric Oxide
Cell Line
Birds
03 medical and health sciences
Immune system
SDG 3 - Good Health and Well-being
medicine
Escherichia coli
Animals
Immunologic Factors
Amino Acid Sequence
L-Lactate Dehydrogenase
Macrophages
lcsh:R
Organic Chemistry
Chemical Compounds
Organisms
Biology and Life Sciences
Proteins
Cell Biology
Molecular Development
Peptide Fragments
030104 developmental biology
Amino Acid Substitution
Gene Expression Regulation
Cell culture
Fowl
Immune System
Mutation
biology.protein
lcsh:Q
Reactive Oxygen Species
Chickens
030215 immunology
Neuroscience
Developmental Biology
Antimicrobial Cationic Peptides
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....e4ce8d4c5be2ae495243c1ee5c35f014