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Vitiligo is a common acquired disease of pigment loss. In lesions recalcitrant to non-invasive treatment, transplantation of cultured autologous melanocytes is an emerging choice. Conventionally, the recipient site is often prepared by laser-mediated or mechanical dermabrasion. Such preparation procedures have disadvantages including prolonged transplantation duration, long period for reepithelialization and potential scarring. We propose a method of preparing recipient sites by psoralen and controlled ultraviolet A (PUVA)-induced blistering followed by transplanting suspended melanocytes. We introduced this method in 10 patients with segmental vitiligo on their recipient site 3 to 5 days before transplantation and blistering developed in 2 to 3 days afterwards. On the day of transplantation, the blister roof could be peeled off easily without bleeding and the recipient site preparation could be completed in 20 min. The recipient site became reepithelialized within 1 week. Progressive repigmentation was observed for up to 6 months, with an average of 65.06% repigmentation in the recipient site without scarring at the end of follow-up. Hence, preparation of the recipient site by controlled PUVA-induced sunburn-like blistering can potentially facilitate melanocyte transplantation and prevent scarring.

Vitiligo is a common acquired disease of pigment loss. In lesions recalcitrant to non-invasive treatment, transplantation of cultured autologous melanocytes is an emerging choice. Conventionally, the recipient site is often prepared by laser-mediated or mechanical dermabrasion. Such preparation procedures have disadvantages including prolonged transplantation duration, long period for reepithelialization and potential scarring. We propose a method of preparing recipient sites by psoralen and controlled ultraviolet A (PUVA)-induced blistering followed by transplanting suspended melanocytes. We introduced this method in 10 patients with segmental vitiligo on their recipient site 3 to 5 days before transplantation and blistering developed in 2 to 3 days afterwards. On the day of transplantation, the blister roof could be peeled off easily without bleeding and the recipient site preparation could be completed in 20 min. The recipient site became reepithelialized within 1 week. Progressive repigmentation was observed for up to 6 months, with an average of 65.06% repigmentation in the recipient site without scarring at the end of follow-up. Hence, preparation of the recipient site by controlled PUVA-induced sunburn-like blistering can potentially facilitate melanocyte transplantation and prevent scarring. [ABSTRACT FROM AUTHOR]

In this study, a simple pyramid-like ultra-wideband absorber was designed to explore high absorptivity across a wide bandwidth. The absorber consisted of eight layers organized into four groups, and each group comprised a metal layer followed by an oxide layer, both of which were square with equal side lengths. Specifically, the chosen oxides, arranged from bottom to top, included SiO2 (t7 layer), Al2O3 (t5 layer), SiO2 (t3 layer), and Al2O3 (t1 layer). In the initial design phase, the thickness of the t8 Ti layer was set to 50 nm and assigned initial values to the thicknesses of the t7-t1 layers, and the widths of the four groups w4, w3, w2, and w1, decreased successively from bottom to top, creating a structure reminiscent of a pyramid. Comsol (version 6.0) was utilized to simulate and systematically vary one parameter at a time, ranging from the thicknesses of the t7-t1 layers to the widths of w4-w1, in order to identify the most suitable structural parameters. Our analyses demonstrated that multimode resonance arose due to the emergence of absorption peaks at lower wavelengths between larger and smaller areas. Additionally, surface plasmon resonance and interference effects between various layers and materials were attributed to the alternating arrangement of metal and oxide layers. The enhancements in the electric field observed at different resonance peak wavelengths illustrated the Fabry–Perot cavity effect, while the impedance matching effect was observed through variations in the real and imaginary parts of the optical impedance with respect to the wave vector. After simulating using these optimally found thicknesses and widths, the aforementioned effects manifested in the pyramid-like ultra-wideband absorber we designed, with its absorptivity surpassing 0.900 across the spectrum from ultraviolet A (335 nm) to middle infrared (4865 nm). [ABSTRACT FROM AUTHOR]

Abstract Background The different wavelengths of solar radiation incident on earth [herein: Photosynthetically Active Radiation (PAR) , Ultra Violet-A (UV-A) and Ultra Violet-B (UV-B)] and their spectral balance not only have an impact on plants’ growth, morphology and physiology, but also are important for the quality and quantity of plant secondary metabolites. Material and methods In an outdoor study we addressed the effects of PAR intensity and UV-A and UV-B on the growth, yield, phenolic and flavonoid content, antioxidant activity and essential oil composition of Pelargonium graveolens L’Hér. The experiment was performed with split plots in a randomized complete block design with three replications. During the growth, two PAR intensities (ambient PAR and reduced PAR) and four UV treatments (ambient UV, enhanced UV-A, enhanced UV-B and enhanced UVA + B) were applied. Results High PAR intensity decreased the length and width of leaf, the height of plant and fresh weight of aerial parts, and increased the dry weight of aerial parts. Enhanced UV-B irradiation was associated with reduced plant height, leaf expansion and fresh and dry weight of aerial parts. Interestingly, the negative effect of UV-B radiation on morphology and growth of plant was largely alleviated by high PAR intensity. The amount of total phenols and flavonoids, antioxidant activity and essential oil production of P. graveolens strongly increased with the increase of UV-B irradiation and PAR. On the other hand, UV-A radiation did not significantly influence total phenol and flavonoid content, antioxidant activity and essential oil composition. Moreover, the combination of high PAR intensity and UV-B led to further increases in total flavonoid content and antioxidant capacity. Both high PAR intensity and enhanced UV-B increased the percentage of geraniol in essential oil, leading to a slight reduction of citronellol/geraniol ratio which is a marker of quality for rose geranium essential oil. Conclusions Overall, we conclude that UV-B irradiation was associated to reduction of plant growth and yield, while, the adverse effect of UV-B irradiation on the plant was mitigated by high PAR intensity. On the other hand, both high PAR and enhanced UV-B boosted the production of phenols, flavonoids and essential oil. Considering that the lower citronellol/geraniol ratio is the most important indicator for the economic value of rose geranium essential oil, reducing citronellol/geraniol ratio under enhanced UV-B radiation and/or high PAR is likely to be favorable.

Fisetin is a flavonoid found in plants and has been reported to be effective in various human diseases. However, the effective mechanisms of ultraviolet-A (UVA)-mediated skin damage are not yet clear. In this study, we investigated the protective mechanisms of fisetin regarding UVA-induced human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) damages. Fisetin showed a cytoprotective effect against UVA irradiation and suppressed matrix metalloproteinases (MMPs), MMP-1, and MMP-3 expression. In addition, fisetin was rescued, which decreased mRNA levels of pro-inflammatory cytokines, reactive oxygen species production, and the downregulation of MAPK/AP-1 related protein and NADPH oxidase (NOX) mRNA levels. Furthermore, UVA-induced MMP-1 and MMP-3 were effectively inhibited by siRNAs to NOX 1 to 5 in HDFs and HEKs. These results indicate that fisetin suppresses UVA-induced damage through the NOX/ROS/MAPK pathway in HDFs and HEKs. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study is to investigate the mechanism of skin adaptation to low-dose ultraviolet A (UVA), changes in mitochondrial reactive oxygen species (ROS) levels, and mitochondrial DNA 4977-bp deficiency (ΔmtDNA4977) in fibroblasts, keratinocytes, and melanocytes. Background: The mechanisms related to the adaptive response of low doses of UVA in the skin remain to be studied, which could provide a basis for light avoidance and treatment. Methods: Skin cells were cultured with different doses of UVA. Laser confocal scanning microscopy was used to observe mitochondrial fluorescence intensity following MitoTracker Red CMX Ros and MitoTracker Red CM-H2X Ros staining. Additionally, the effects of different cumulative UVA irradiation doses on the mitochondrial number and ROS levels were determined. The total mtDNA mutation level and incidence of ΔmtDNA4977 were comparatively quantified using the real-time polymerase chain reaction. Results: Cells exposed to low-dose UVA showed changes in cell aging, and acute toxic reactions were observed in cells exposed to high-dose UVA. The acute toxic reactions were less severe in the adaptive response group than in the high-dose group. UVA irradiation could induce mitochondrial ROS production. Intracellular ROS levels increased swiftly following high-dose UVA irradiation, while low-dose UVA irradiation induced a steady low-level increase in intracellular ROS compared with the sham irradiation group (p < 0.01). Conclusions: Low-dose UVA irradiation exposure induced a sustained low level of mitochondrial ROS production. Besides signal transduction, the adaptive response may be triggered and maintained by ΔmtDNA4977, resulting in a low level of mitochondrial ROS. [ABSTRACT FROM AUTHOR]

Background: The different wavelengths of solar radiation incident on earth [herein: Photosynthetically Active Radiation (PAR) , Ultra Violet-A (UV-A) and Ultra Violet-B (UV-B)] and their spectral balance not only have an impact on plants' growth, morphology and physiology, but also are important for the quality and quantity of plant secondary metabolites. Material and methods: In an outdoor study we addressed the effects of PAR intensity and UV-A and UV-B on the growth, yield, phenolic and flavonoid content, antioxidant activity and essential oil composition of Pelargonium graveolens L'Hér. The experiment was performed with split plots in a randomized complete block design with three replications. During the growth, two PAR intensities (ambient PAR and reduced PAR) and four UV treatments (ambient UV, enhanced UV-A, enhanced UV-B and enhanced UVA + B) were applied. Results: High PAR intensity decreased the length and width of leaf, the height of plant and fresh weight of aerial parts, and increased the dry weight of aerial parts. Enhanced UV-B irradiation was associated with reduced plant height, leaf expansion and fresh and dry weight of aerial parts. Interestingly, the negative effect of UV-B radiation on morphology and growth of plant was largely alleviated by high PAR intensity. The amount of total phenols and flavonoids, antioxidant activity and essential oil production of P. graveolens strongly increased with the increase of UV-B irradiation and PAR. On the other hand, UV-A radiation did not significantly influence total phenol and flavonoid content, antioxidant activity and essential oil composition. Moreover, the combination of high PAR intensity and UV-B led to further increases in total flavonoid content and antioxidant capacity. Both high PAR intensity and enhanced UV-B increased the percentage of geraniol in essential oil, leading to a slight reduction of citronellol/geraniol ratio which is a marker of quality for rose geranium essential oil. Conclusions: Overall, we conclude that UV-B irradiation was associated to reduction of plant growth and yield, while, the adverse effect of UV-B irradiation on the plant was mitigated by high PAR intensity. On the other hand, both high PAR and enhanced UV-B boosted the production of phenols, flavonoids and essential oil. Considering that the lower citronellol/geraniol ratio is the most important indicator for the economic value of rose geranium essential oil, reducing citronellol/geraniol ratio under enhanced UV-B radiation and/or high PAR is likely to be favorable. [ABSTRACT FROM AUTHOR]

This study investigated the effects of substituting B with FR and UVA (50 μmol·m−2·s−1) at two growth stages on the growth and quality of loose-leaf lettuce (cv. Fangni). The basal light was red and blue LEDs at 250 μmol·m−2·s−1. At stage I (the first 10 days of 20-day pre-harvest), there were three treatments: B substituted by FR (FR); B substituted by UVA (UVA); and no substituting (CKI). At stage II (next ten days after stage I), there were 9 treatments: FF (FR + FR), UF (UVA + FR), BF (B + FR), FU (FR + UVA), UU (UVA + UVA), BU (B + UVA), FB (FR + B), UB (UVA + B), and B + B (CKII). At stage I, compare with UV-A and CKI, the shoot fresh weight, leaf area, leaf width, leaf length, and vitamin C content highly increased under far-red light (FR), whereas specific leaf weight and the chlorophyll contents significantly decreased by FR. In CKI, nitrate contents and the antioxidant capacity (FRAP, DPPH) were significantly higher than in FR and UVA. At stage II, higher leaf width, leaf length, leaf area, and shoot fresh and dry weight were observed in FF, UF, and BF. The lowest biomass was shown in CKII. Higher chlorophyll contents were found in FU and FB. The soluble sugar contents significantly increased by all treatments. In addition to UB and BU, soluble protein contents increased by other treatments. There were higher vitamin C contents in UU, UB, and CKII. Large amounts of nitrates accumulated under CKII. The higher antioxidant capacity (DPPH, FRAP) was found in FB and CKII. The highest flavonoid content was found in UB, and higher polyphenols contents were found in UB and BU. In this study, substituting B with FR at 2 stages were the best way to increase lettuce biomass. The optimal measure to both increased lettuce nutrition quality and biomass was FB at stage II. [ABSTRACT FROM AUTHOR]

In this study, a simple pyramid-like ultra-wideband absorber was designed to explore high absorptivity across a wide bandwidth. The absorber consisted of eight layers organized into four groups, and each group comprised a metal layer followed by an oxide layer, both of which were square with equal side lengths. Specifically, the chosen oxides, arranged from bottom to top, included SiO2 (t7 layer), Al2O3 (t5 layer), SiO2 (t3 layer), and Al2O3 (t1 layer). In the initial design phase, the thickness of the t8 Ti layer was set to 50 nm and assigned initial values to the thicknesses of the t7-t1 layers, and the widths of the four groups w4, w3, w2, and w1, decreased successively from bottom to top, creating a structure reminiscent of a pyramid. Comsol (version 6.0) was utilized to simulate and systematically vary one parameter at a time, ranging from the thicknesses of the t7-t1 layers to the widths of w4-w1, in order to identify the most suitable structural parameters. Our analyses demonstrated that multimode resonance arose due to the emergence of absorption peaks at lower wavelengths between larger and smaller areas. Additionally, surface plasmon resonance and interference effects between various layers and materials were attributed to the alternating arrangement of metal and oxide layers. The enhancements in the electric field observed at different resonance peak wavelengths illustrated the Fabry–Perot cavity effect, while the impedance matching effect was observed through variations in the real and imaginary parts of the optical impedance with respect to the wave vector. After simulating using these optimally found thicknesses and widths, the aforementioned effects manifested in the pyramid-like ultra-wideband absorber we designed, with its absorptivity surpassing 0.900 across the spectrum from ultraviolet A (335 nm) to middle infrared (4865 nm).

BackgroundSummer and spring eruptions on the elbows are a variant of polymorphous light eruption described on clinical and histopathological grounds; however, to our knowledge, they have not been confirmed by photobiological studies.ObjectiveBased on photobiological studies, this study aimed to demonstrate the involvement of ultraviolet-A (UVA) radiation in this variant of polymorphous light eruption occurring exclusively on the elbows.MethodsA series of five patients with polymorphous light eruption lesions on the elbows were included in our study. All patients underwent phototesting and photoprovocation of the skin lesions after exposure to a UVA light source [Philips UVA HPA lamp (400 W)]. All patients underwent punch biopsy and histopathological and immunohistochemical studies with anti-CD123.ResultsIn all the cases, UVA irradiation caused the appearance of skin lesions on the elbows with characteristic polymorphous light eruption. Histological data showed edema in the superficial dermis and a perivascular lymphocytic infiltrate compatible with polymorphous light eruption. Immunohistochemical staining for CD1-23 showed negative results.ConclusionsFor the first time, photobiological photoprovocation studies demonstrated that repeated exposure to UVA radiation leads to the generation of skin lesions on the elbows, which are clinically and histologically consistent with summer and spring eruptions, confirming that elbow rash is a variant of polymorphous light eruption.

Background: Lysosomal cathepsin D (CTSD) can degrade internalized advanced glycation end products (AGEs) in dermal fibroblasts. CTSD expression is decreased in photoaged fibroblasts, which contributes to intracellular AGEs deposition and further plays a role in AGEs accumulation of photoaged skin. The mechanism under downregulated CTSD expression is unclear. Objective: To explore possible mechanism of regulating CTSD expression in photoaged fibroblasts. Methods: Dermal fibroblasts were induced into photoaging with repetitive ultraviolet A (UVA) irradiation. The competing endogenous RNA (ceRNA) networks were constructed to predict candidate circRNAs or miRNAs related with CTSD expression. AGEs‐BSA degradation by fibroblasts was studied with flow cytometry, ELISA, and confocal microscopy. Effects of overexpressing circRNA‐406918 via lentiviral transduction on CTSD expression, autophagy, AGE‐BSA degradation were analyzed in photoaged fibroblasts. The correlation between circRNA‐406918 and CTSD expression or AGEs accumulation in sun‐exposed and sun‐protected skin was studied. Results: CTSD expression, autophagy, and AGEs‐BSA degradation were significantly decreased in photoaged fibroblasts. CircRNA‐406918 was identified to regulate CTSD expression, autophagy, and senescence in photoaged fibroblasts. Overexpressing circRNA‐406918 potently decreased senescence and increased CTSD expression, autophagic flux, and AGEs‐BSA degradation in photoaged fibroblasts. Moreover, circRNA‐406918 level was positively correlated with CTSD mRNA expression and negatively associated with AGEs accumulation in photodamaged skin. Further, circRNA‐406918 was predicted to mediate CTSD expression through sponging eight miRNAs. Conclusion: These findings suggest that circRNA‐406918 regulates CTSD expression and AGEs degradation in UVA‐induced photoaged fibroblasts and might exert a role in AGEs accumulation in photoaged skin. [ABSTRACT FROM AUTHOR]

Yuanyuan Jia,1 Qiuyu Mao,1 Jingyi Yang,1 Na Du,1 Yuan Zhu,2 Wei Min1 1First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 2First People’s Hospital of Changshu City, Changshu Hospital Affiliated of Soochow University, Changshu, Jiangsu Province, People’s Republic of ChinaCorrespondence: Wei Min, Email weimin@suda.edu.cnBackground: Ultraviolet (UV) is a common stressor of skin and repeated UVA radiation contributes to photoaging. (–)-Epigallocatechin-3-Gallate (EGCG), as the major polyphenol that is found in green tea, and catechins and have shown considerable antioxidant capacity.Purpose: Our study aims to explore the effects of EGCG on UVA-induced skin photoaging process and associated mechanisms.Methods: In this study, human skin fibroblasts (HSFs) were treated with UVA and EGCG, and subsequent changes in cell morphology, telomeres, antioxidant capacity, cell cycle, and related genes were evaluated to examine the role and mechanisms of EGCG in delaying skin photoaging.Results: HSF exposed to UVA underwent an increase in aging-related biomarkers and telomere shortening. Also, UVA radiation inhibited the secretion of transforming growth factor-beta1 (TGF-β 1), induced cell cycle arrest, down-regulated antioxidant enzymes, and promoted the accumulation of oxidative product malondialdehyde (MDA) to cause further damage to cells. Increased expression of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinase-1 (TIMP-1), p66 at mRNA levels were also observed after UVA irradiation. EGCG treatment effectively inhibited above damage processes caused by UVA radiation in HSF.Conclusion: Our study indicated that the potential mechanism of EGCG retarding photoaging is closely related to its powerful antioxidant effects and the ability to regulate the expression of related genes, and the usage of EGCG will be a potential strategy in preventing skin photoaging induced by UVA radiation.Keywords: ultraviolet A, human skin fibroblasts, (–)-Epigallocatechin-3-Gallate, EGCG, photoaging

This study was conducted to evaluate scleral remolding-related gene expression after scleral collagen cross-linking (SCXL) using ultraviolet A (UVA) and riboflavin in lens-induced myopia (LIM) guinea pigs. A total of 100 4-week-old pigmented guinea pigs were randomly divided into five groups (n = 20): SCXL + LIM, LIM, SCXL, Sham, and Control. Refraction, anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), and axial length (AL) were measured using streak retinoscope and A-scan ultrasonography. SCXL was performed using 0.1% riboflavin solution and 365 nm UVA irradiation. Lens-induced myopia was achieved by wearing −10 D concave lenses. Quantitative real-time PCR (qPCR) and western blot were used to measure mRNA and protein levels, respectively. Myopia was successfully induced in the LIM group, while myopic refraction was higher and ACD and AL were shorter in SCXL + LIM compared with LIM, suppressing myopia progression. The scleral COL1A1 mRNA levels were significantly decreased and MMP2 and ACTA2 mRNA levels were significantly increased in LIM compared with other groups, while COL1A1 mRNA levels were increased and MMP2 and ACTA2 mRNA levels were decreased in SCXL + LIM compared with LIM. The scleral COL1A1 protein levels were significantly increased at 1 week and 4 weeks and MMP2 protein levels were significantly decreased at 1 week in SCXL compared with SCXL + LIM, LIM and Control. MMP2 protein levels were significantly decreased in SCXL + LIM and SCXL compared with LIM at 4 weeks. The differences in TGFB1, BMP2, CCN2, ITGA2, and ITGB1 mRNA levels and ACTA2 protein levels between the five groups were not significantly different. SCXL using UVA and riboflavin could influence the expression of scleral remolding-related genes, including COL1A1, MMP2, TIMP2, and ACTA2, and thus contribute to improving collagen synthesis and reducing collagen degradation and might have an effect on slowing myopia progression. [ABSTRACT FROM AUTHOR]

Photoaging is mainly caused by the exposure of the skin to ultraviolet (UV) radiation. Among them, damage to human dermal fibroblast (HDF) cells caused by ultraviolet A (UVA) is the main cause of skin aging. Researchers have dedicated to identifying natural compounds from plants to fight against UV radiation-induced photoaging. We previously found that extracts from wild chrysanthemum could prevent acute damage and photoaging induced by UV irradiation. As one of the most abundant ingredients in wild chrysanthemum extract, handelin was hypothesized to have the potential to prevent UVA-induced photoaging of skin fibroblast. In the present study, we report the great potential of handelin in combating UVA-induced photoaging of fibroblasts. We firstly demonstrated that handelin was safe for skin fibroblast as high as a concentration of 0.0125 µ M, showing no toxicity on the cells and improved cell viability. Furthermore, handelin can reduce UVA-induced cellular senescence, indicated by a reduced proportion of senescence-associated beta-galactose positive cells and the expression of P21. We then verified that handelin pretreatment markedly attenuated the production of reactive oxygen species (ROS) generation after UVA irradiation. Meanwhile, we found that handelin enhances autophagy after UVA irradiation, and autophagy is involved in the quality control of intracellular proteins after UV-induced damage (partially indirectly via ROS). Therefore, these results suggest that handelin has a very high potential as an effective ingredient against UVA-induced skin aging. Moreover, this provides an important basis for further research on the photoprotective mechanism of handelin. [ABSTRACT FROM AUTHOR]

Previous studies have highlighted the importance of outdoor time in reducing the risk of myopia progression. Although ultraviolet A (UVA) radiation dominates in terms of energy with respect to the UV radiation reaching the Earth's surface, its effects on the exposed anterior sclera have not been well studied. This study was designed to investigate the UVA-induced biological effects at peak sunlight levels in human scleral fibroblasts (HSFs). Using next-generation sequencing (NGS), we analyzed the differentially expressed genes (DEGs) in UVA-treated and normal HSFs. Further, we then identified the functions and key regulators of the DEGs using bioinformatics analysis, and verified the effects of UVA on gene and protein expression in HSFs using real-time PCR, western blotting, and immunofluorescence imaging. The highest level of solar UVA (365 nm) was 3.4 ± 0.18 (mW/cm2). The results from the functional analysis of the DEGs were related to structural changes in the extracellular matrix (ECM) and protein metabolism. Transforming growth factor-β1 (TGF-β1) and Smad3 were predicted to be potential upstream regulators, associated with ECM organization. Exposure to a single wavelength of UVA (365 nm, 3 mW/cm2) for 1 h for 5 consecutive days induced the downregulation of the mRNA of ECM genes including COL1A1, COL3A1, COL5A1, VCAN and collagen I protein in HSF. UVA downregulated Smad3 protein and reduced TGF-β-induced collagen I protein production following UVA exposure in HSF. In conclusion, high UVA exposure reduces TGF-β signaling and collagen I production by modulating Smad levels in HSF. The effects of overexposure to high-intensity UVA on myopia control require further investigations. • Solar noon ultraviolet A level suppresses scleral collagen synthesis. • Ultraviolet A down-regulated TGF-β/Smad3 signaling in human scleral fibroblasts. • Outdoor activities at noon should be arranged cautiously regarding myopia control. [ABSTRACT FROM AUTHOR]

This study investigated the effects of substituting B with FR and UVA (50 μmol·m−2·s−1) at two growth stages on the growth and quality of loose-leaf lettuce (cv. Fangni). The basal light was red and blue LEDs at 250 μmol·m−2·s−1. At stage I (the first 10 days of 20-day pre-harvest), there were three treatments: B substituted by FR (FR); B substituted by UVA (UVA); and no substituting (CKI). At stage II (next ten days after stage I), there were 9 treatments: FF (FR + FR), UF (UVA + FR), BF (B + FR), FU (FR + UVA), UU (UVA + UVA), BU (B + UVA), FB (FR + B), UB (UVA + B), and B + B (CKII). At stage I, compare with UV-A and CKI, the shoot fresh weight, leaf area, leaf width, leaf length, and vitamin C content highly increased under far-red light (FR), whereas specific leaf weight and the chlorophyll contents significantly decreased by FR. In CKI, nitrate contents and the antioxidant capacity (FRAP, DPPH) were significantly higher than in FR and UVA. At stage II, higher leaf width, leaf length, leaf area, and shoot fresh and dry weight were observed in FF, UF, and BF. The lowest biomass was shown in CKII. Higher chlorophyll contents were found in FU and FB. The soluble sugar contents significantly increased by all treatments. In addition to UB and BU, soluble protein contents increased by other treatments. There were higher vitamin C contents in UU, UB, and CKII. Large amounts of nitrates accumulated under CKII. The higher antioxidant capacity (DPPH, FRAP) was found in FB and CKII. The highest flavonoid content was found in UB, and higher polyphenols contents were found in UB and BU. In this study, substituting B with FR at 2 stages were the best way to increase lettuce biomass. The optimal measure to both increased lettuce nutrition quality and biomass was FB at stage II.

Ultraviolet A (UVA) radiation can pass through the epidermis and reach the dermal skin layer, contributing to photoaging, DNA damage, and photocarcinogenesis in dermal fibroblasts. High-dose UVA exposure induces erythema, whereas low-dose, long-term UVA exposure causes skin damage and cell senescence. Biomarkers for evaluating damage caused by low-dose UVA in fibroblasts are lacking, making it difficult to develop therapeutic agents for skin aging and aging-associated diseases. We performed RNA-sequencing to investigate gene and pathway alterations in low-dose UVA-irradiated human skin-derived NB1RGB primary fibroblasts. Differentially expressed genes were identified and subjected to Gene Ontology and reactome pathway analysis, which revealed enrichment in genes in the senescence-associated secretory phenotype, apoptosis, respiratory electron transport, and transcriptional regulation by tumor suppressor p53 pathways. Insulin-like growth factor binding protein 7 (IGFBP7) showed the lowest p-value in RNA-sequencing analysis and was associated with the senescence-associated secretory phenotype. Protein–protein interaction analysis revealed that Fos proto-oncogene had a high-confidence network with IGFBP7 as transcription factor of the IGFBP7 gene among SASP hit genes, which were validated using RT-qPCR. Because of their high sensitivity to low-dose UVA radiation, Fos and IGFBP7 show potential as biomarkers for evaluating the effect of low-dose UVA radiation on dermal fibroblasts. [ABSTRACT FROM AUTHOR]

Background: New research has attributed increased significance to the causal link between ultraviolet A (UVA) radiation and immunosuppression and carcinogenesis. In the United States, sunscreens are labeled with only their sun protection factor (SPF) and an imprecise term "broad‐spectrum protection." Sunscreen marketing and efficacy evaluations continue to be based primarily on skin redness (sunburn) or erythema. We sought to evaluate the ultraviolet (UV) protection offered by common sunscreen products on the US market using laboratory‐measured UV‐absorption testing and comparing with computer‐modeled protection and the labeled SPF values. This approach enables an investigation of the relationship between the labeled SPF and measured UVA protection, a factor that is ignored in current regulations. Methods: Fifty‐one sunscreen products for sale in the United States with SPF values from 15 to 110 and labeled as providing broad‐spectrum protection were tested using a commercial laboratory. All products were evaluated using the ISO 24443:2012 method for sunscreen effectiveness. The final absorbance spectra were used for analysis of in vitro UV protection. Results: In vitro SPF values from laboratory‐measured UV absorption and computer modeling were on average just 59 and 42 percent of the labeled SPF. The majority of products provided significantly lower UVA protection with the average unweighted UVA protection factor just 24 percent of the labeled SPF. Conclusion: Regulations and marketplace forces promote sunscreens that reduce sunburn instead of products that provide better, more broad‐spectrum UV protection. The production and use of products with broad spectrum UV protection should be incentivized, removing the emphasis on sunburn protection and ending testing on people. [ABSTRACT FROM AUTHOR]

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To compare the effectiveness and differences in slowing myopia progression in Guinea pigs by riboflavin/ultraviolet A (UVA) scleral cross-linking (sCXL) before and after lens-induced myopia (LIM). Forty 4-week-old Guinea pigs were randomly divided into four groups (n = 10 per group): CXL-A, CXL-B, LIM, and Control groups. The right eyes in CXL-A, CXL-B, LIM groups were treated with −10.00 D lenses from 4 to 10-week old and the left eyes were untreated. In CXL-A and CXL-B groups, riboflavin/UVA sCXL was performed on the right eyes at 4 weeks and 8 weeks of age, respectively. Both eyes were untreated in Control group. The intraocular pressure (IOP), the axial length (AXL), and the refraction were measured in vivo at 4, 8, and 10 weeks of age. At 10 weeks of age, the right eyes were enucleated for the tensile test and transmission electron microscopy observations. The myopia has been successfully induced in LIM and CXL-B groups during 4–8 weeks. In CXL-A group, the growth rate of AXL and myopic refraction was markedly inhibited during 4–8 weeks and the inhibitory effects diminished during 8–10 weeks. During 8–10 weeks, the growth rate of AXL and myopic refraction in CXL-B were marked suppressed. At 10 weeks of age, the myopia refraction was lower and the AXL was shorter in CXL-A group in comparison to CXL-B group. The IOP was not significantly different among the 4 groups of eyes at 4, 8, and 10 weeks of age. The scleral stiffness, the fibril diameters, and the fibril density of the sclera were significantly increased in CXL-A and CXL-B groups compared to LIM group. Riboflavin/UVA sCXL administrated before and after the myopia modeling could both slow the myopia progression in Guinea pigs. The before-myopia preventative sCXL showed lower myopic refraction in the same age comparison between the cross-linked groups. The effect of riboflavin/UVA sCXL might reduce over time and the long-term effect should be further investigated. This sCXL intervention might control the ultrastructure alterations of the sclera during the myopia remodeling. [ABSTRACT FROM AUTHOR]

BackgroundPUVA treatment is photochemotherapy for psoriasis that combines psoralen with UVA radiation. Although PUVA is a very effective treatment option for psoriasis, there is an absence of patient resources explaining and demonstrating the process of PUVA. Studies have shown that patients who viewed videos explaining the treatment procedures for various medical conditions had a greater understanding of their treatment and were more active participants in their health.ObjectiveTo present a freely available online guide and video on PUVA treatment designed for patient education on PUVA.MethodsThe PUVA treatment protocol used at the University of California-San Francisco Psoriasis and Skin Treatment Center as well as available information from the literature was reviewed to design a comprehensive guide for patients receiving PUVA treatment.ResultsWe created a printable guide and video resource that reviews the benefits and risks of PUVA, discusses the three types of PUVA (hand-foot soak, full body soak, and systemic), demonstrates the PUVA process, and provides practical tips for safe use.ConclusionOnline media and video delivers material in a way that is flexible and often familiar to patients. This new format is beneficial for prospective patients planning to undergo PUVA treatment, health-care providers, and trainees who want to learn more about this treatment.

To investigate the effects of iontophoresis–ultraviolet A (UVA) cross-linking (CXL) with hypotonic riboflavin solution on the ultrastructural changes in the lamellae, collagen fibrils (CFs), and proteoglycans (PGs) in the central and peripheral stroma of the human corneal buttons. The iontophoresis method was used for the trans -epithelial application of hypotonic riboflavin in ex vivo corneal culture for 5 min. The corneas were irradiated using three methods: Group 1 (G1) , a UVA irradiance of 3 mW/cm2 for 30 min; Group 2 (G2) , a UVA irradiance of 10 mW/cm2 for 9 min; Group 3 (G3) , without UVA irradiation. Three untreated corneas were used as controls ( G0 ). After the CXL procedure, the corneas were processed for electron microscopy. The CF diameter and PGs in each sample were analyzed using the iTEM program. The keratocyte organelles and stromal architecture in the peripheral cornea were better preserved than those in the central cornea. In G1 and G2 , the mean CF diameter in the peripheral cornea was significantly higher than that in the central cornea. In G3 , the CF diameter in the central cornea was significantly larger than that in the peripheral cornea. Furthermore, differences in PG area size were observed between the central and peripheral corneas in all groups. Riboflavin + UVA application at 3 mW/cm2 for 30 min and 10 mW/cm2 for 9 min was a suitable method of CXL; however, 3 mW/cm2 for 30 min improved the organization and size of the collagen fibrils. CXL treatment applied at the periphery was more effective than that applied at the center. [ABSTRACT FROM AUTHOR]

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The use of sunscreens is recommended to limit the impact of sun exposure on the skin. The objective of this study was to examine the relationship between sunscreen sales and melanoma in 4 different countries with diverse sunscreen regulations. Data from publicly avail­able databases were examined for Sweden, England, Australia, and the USA from 1999 to 2018. The association between incidence of melanoma and sunscreen sales was estimated using a generalized estimating equation, and modelling was used to predict melanoma cases. Incidence of melanoma was positively associated with sunscreen sales in England, Australia, and the USA, and negatively associated with sunscreen sales in Sweden. Growth rates in melanoma cases of 0.42%, 16.7%, 19.1% and 12.2% were predicted for Sweden, England, Australia, and the USA, respectively. The differences observed between England, Australia, and the USA, on the one hand, and Sweden, on the other hand, are consistent with the adoption of strong regulations requiring the use of ultraviolet A blocking agents in sunscreens.

Polyherbal formulation combining multiple herbs is suggested to achieve enhanced therapeutic effects and reduce toxicity. Harak herbal formula (HRF) extracts were proposed to regulate skin responses to UVR through their ability to suppress UVA-induced matrix metalloproteinase-1 (MMP-1) and pigmentation via promoting antioxidant defenses in in vitro models. Therefore, natural products targeting Nrf2 (nuclear factor erythroid 2-related factor 2)-regulated antioxidant response might represent promising anti-photoaging candidates. Hesperetin (HSP) was suggested as a putative bioactive compound of the HRF, as previously shown by its chemical profiling using the liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). In this study, we explored the anti-photoaging effects of HRF extracts and HSP on normal human dermal fibroblasts (NHDFs) and mouse skin exposed to UVA irradiation. Pretreatment of NHDFs with HRF extracts and HSP protected against UVA (8 J/cm2)-mediated cytotoxicity and reactive oxygen species (ROS) formation. The HRF and HSP pretreatment also attenuated the UVA-induced MMP-1 activity and collagen depletion concomitant with an upregulation of Nrf2 activity and its downstream genes (GST and NQO-1). Moreover, our findings provided the in vivo relevance to the in vitro anti-photoaging effects of HRF as topical application of the extracts (10, 30 and 100 mg/cm2) and HSP (0.3, 1, and 3 mg/cm2) 1 h before UVA exposure 3 times per week for 2 weeks (a total dose of 60 J/cm2) mitigated MMP-1 upregulation, collagen loss in correlation with enhanced Nrf2 nuclear accumulation and its target protein GST and NQO-1 as well as reduced 8-hydroxy-2′-deoxyguanosine (8-OHdG) in irradiated mouse skin. Thus, our findings revealed that HRF extracts and HSP attenuated UVA-induced photoaging via upregulating Nrf2, together with their abilities to reduce ROS formation and oxidative damage. Our study concluded that the HRF and its bioactive ingredient HSP may represent potential candidates for preventing UVA-induced photoaging via restoration of redox balance.

The use of sunscreens is recommended to limit the impact of sun exposure on the skin. The objective of this study was to examine the relationship between sunscreen sales and melanoma in 4 different countries with diverse sunscreen regulations. Data from publicly available databases were examined for Sweden, England, Australia, and the USA from 1999 to 2018. The association between incidence of melanoma and sunscreen sales was estimated using a generalized estimating equation, and modelling was used to predict melanoma cases. Incidence of melanoma was positively associated with sunscreen sales in England, Australia, and the USA, and negatively associated with sunscreen sales in Sweden. Growth rates in melanoma cases of 0.42%, 16.7%, 19.1% and 12.2% were predicted for Sweden, England, Australia, and the USA, respectively. The differences observed between England, Australia, and the USA, on the one hand, and Sweden, on the other hand, are consistent with the adoption of strong regulations requiring the use of ultraviolet A blocking agents in sunscreens. [ABSTRACT FROM AUTHOR]

Chronic actinic dermatitis (CAD) is a common debilitating photodermatosis. Patients often have to completely avoid outdoor activities, which severely impacts their quality of life. Phototherapy is effective for CAD and seems to increase patients' tolerance towards sunlight and consequently decrease the extent of disease. Unfortunately, the slower onset and time‐consuming nature of phototherapy limits the clinical application. Considering the effectiveness and time‐saving nature of ultraviolet (UV)‐A rush hardening in solar urticaria, we performed a pilot study to determine whether UV‐A rush hardening is effective in CAD. Six patients with CAD were exposed to multiple sessions of UV‐A for 4–5 days at 1‐h intervals/day. Subsequently, maintenance UV‐A exposure was performed at 1–2‐week intervals. Phototesting at baseline showed that three patients were sensitive to both UV‐A and ‐B, and the other three patients only showed UV‐A sensitivity. All of the patients responded well to UV‐A rush hardening and four (67%) maintained a good remission status after 1 year. The results of this pilot study suggest that UV‐A rush hardening phototherapy is effective and well tolerated in the treatment of CAD, while future larger prospective studies using objective scores of disease activity and quality of life are needed. [ABSTRACT FROM AUTHOR]

To comparatively evaluate the long-term visual, refractive, topographic and aberrometric outcomes of conventional corneal collagen crosslinking (CXL) in the management of pediatric versus adult progressive keratoconus patients. Retrospective, cross-sectional review of consecutive progressive keratoconus cases of corneal standard CXL using a standardized protocol for treatment and examinations was performed. Best spectacle-corrected distance visual acuity (CDVA), manifest refraction, slit lamp biomicroscopy, corneal tomography, corneal aberrometry and endothelial cell counts were evaluated at baseline and yearly at all postoperative follow-up examinations after month-12. The outcomes were analyzed by dividing the patients into 2 age groups; pediatric (≤18 years) versus adult (>18 years). Eighty-eight eyes (54 patients) in the pediatric and 104 eyes (68 patients) in the adult age groups completed 3 years follow-up. Compared to baseline, the mean CDVA, manifest refraction, keratometric readings, tomographic and aberrometric parameters improved statistically significantly in both groups at postoperative year-3 and year-4, without any statistically significant between-group differences. No change in the mean endothelial cell density (p >.05), or no sight threating complication was encountered in any patient eye in either group. In a subset of patients who were followed for 4 years (71 eyes in the pediatric and 70 eyes in the adult age groups), the outcome analyses were again similar between-groups. Conventional corneal CXL effectively halts the progression of keratoconus in both pediatric and adult age groups in long-term follow-up with similar visual, refractive, tomographic and aberrometric efficacy in both groups. [ABSTRACT FROM AUTHOR]

Background/Purpose: To examine the efficacy, tolerability and safety of phototherapy in children, in whom there is currently a paucity of data. Materials and Methods: Retrospective review of children under 18 years who received narrowband UVB (NB‐UVB), broadband UVB (BB‐UVB) phototherapy or psoralen with UVA (PUVA) photochemotherapy between 2003 and 2017 at a tertiary Paediatric dermatology centre in Southampton, UK. Results: 100 children aged 6‐17 years were included. The majority of children had psoriasis (74), atopic dermatitis (10) or vitiligo (8), with others having rarer dermatoses. Grade 2 erythema or above occurred in 46% of all included children and 42% (36/86) of those receiving NB‐UVB; however, grade 3 and 4 reactions were infrequent and only 3 children stopped treatment due to burning. NB‐UVB was particularly effective in those with psoriasis; 55/65 (85%) significantly improved, and 72% had not relapsed after 2 years. However, its effectiveness in atopic dermatitis was less convincing; in a small group of children, 6/10 (60%) significantly improved, but 66% relapsed within 3 months. Conclusions: Our analysis demonstrates that NB‐UVB is effective in children with psoriasis and vitiligo, with potential to achieve extended periods of remission in psoriasis. Its usefulness in atopic dermatitis is less clear. The long‐term safety of NB‐UVB in children is still unknown, but it appears to be a well‐tolerated treatment and should be considered in children for a variety of inflammatory dermatoses before progressing to immunosuppressive therapies. [ABSTRACT FROM AUTHOR]

Corneal cross-linking (CXL) is a noninvasive therapeutic procedure for keratoconus that is aimed at improving corneal biomechanical properties by induction of covalent cross-links between stromal proteins. It is accomplished by ultraviolet A (UVA) radiation of the cornea, which is first saturated with photosensitizing riboflavin. It has been shown that standard epithelium-off CXL (S-CXL) is efficacious, and it has been recommended as the standard of care procedure for keratoconus. However, epithelial removal leads to pain, transient vision loss, and a higher risk of corneal infection. To avoid these disadvantages, transepithelial CXL was developed. Recently, iontophoresis has been adopted to increase riboflavin penetration through the epithelium. Several clinical observations have demonstrated the safety and efficacy of iontophoresis-assisted epithelium-on CXL (I-CXL) for keratoconus. This review aimed to provide a comprehensive summary of the published studies regarding I-CXL and a comparison between I-CXL and S-CXL. All articles used in this review were mainly retrieved from the PubMed database. Original articles and reviews were selected if they were related to the I-CXL technique or related to the comparison between I-CXL and S-CXL.

Background: Blood transfusion of contaminated components is a potential source of sepsis by a wide range of known and unknown pathogens. Collection mechanism and storage conditions of platelets make them vulnerable for bacterial contamination. Several interventions aim to reduce the transfusion of contaminated platelet units; however, data suggest that contaminated platelet transfusion remains very common. Aim: A pathogen inactivation system, “INTERCEPT”, to inactivate bacteria in deliberately contaminated platelet units was implemented and evaluated. Materials and Methods: Five single-donor platelets (SDP) and five random donor platelets (RDP) were prepared after prior consent of donors. Both SDP and RDP units were deliberately contaminated by stable stock ATCC Staphylococcus aureus and Escherichia coli, respectively, with a known concentration of stock culture. Control samples were taken from the infected units and bacterial concentrations were quantified. The units were treated for pathogen inactivation with the INTERCEPT (Cerus Corporation, Concord, CA) Blood system for platelets (Amotosalen/UVA), as per the manufacturer's instructions for use. Post illumination, test samples were analyzed for any bacterial growth. Results: Post-illumination test samples did not result in any bacterial growth. A complete reduction of >6 log10S. aureus in SDP units and >6 log10Escherichia coli in RDP units was achieved. Conclusion: The INTERCEPT system has been shown to be very effective in our study for bacterial inactivation. Implementation of INTERCEPT may be used as a mitigation against any potential bacterial contamination in platelet components.

Daily exposure to ultraviolet (UV) light induces inflammation and tumorigenesis in the skin. Silibinin and ellagic acid are natural products that exhibit anti-inflammatory and anti-tumorigenic properties. Insulin receptor substrate protein 1 (IRS1) is important for skin homeostasis and physiology, but its activity following UV radiation remains unclear. We investigated the effects of ellagic acid and silibinin on IRS1 expression in ultraviolet A (UVA) and ultraviolet B (UVB) irradiated rat skin. Forty-two female Wistar rats were divided randomly into six groups of seven animals. The dorsal skin of rats was exposed to UVA + UVB, then treated with ellagic acid and silibinin by gavage. IRS1 expression in skin tissues was determined by western blot analysis. IRS1 expression increased significantly following treatment with ellagic acid and silibinin in UVA + UVB irradiated skin compared to the UVA + UVB only group. After UVA + UVB treatment, ellagic acid effected greater induction of IRS1 expression than silibinin. Our findings suggest that the photoprotective roles of ellagic acid and silibinin may be due to induction of IRS1 expression in UVA + UVB treated rat skin. [ABSTRACT FROM AUTHOR]

Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation. [ABSTRACT FROM AUTHOR]

Purpose: To compare the safety and efficacy of Accelerated Corneal Cross Linking (AXL) protocols, 9 mW/cm² for 10 min with 18 mW/cm² for 5 min in terms of refractive and topographic keratometric indices in patients with progressive Keratoconus. Study Design: Quasi experimental study. Place and Duration of Study: Armed Forces Institute of Ophthalmology, Rawalpindi Pakistan, from Nov 2016 to Jun 2018. Material and Methods: Sixty eyes, 30 in each group, of 55 consecutive patients diagnosed with progressive keratoconus were enrolled through convenience sampling and were subjected to AXL with irradiance protocols of 18 mWatt/cm² for 5 minutes in group 1 and 9 mW/cm² for 10 minutes in group 2. All patients underwent a comprehensive ophthalmic examination at baseline and postoperative follow up visits at 3, 6, 12 and 18 months. Primary outcome parameter was disease stability defined as increase in maximum keratometry over baseline K max of no more than 1 diopter at 1 year after AXL. Statistical analysis of data was performed with IBM SPSS software (version 20.0 SPSS). P value of < 0.05 was considered as statistically significant. Results: Disease stability was 96 % in each group. At the final time point of 18 months, group 2 (AXL 9 mm watt/cm² for 10 min) was superior as compared to group 1 (AXL 18 mm watt/cm2 for 5 min) in terms of flattening of steep and sim K (p = 0.007, 0.023 respectively). Conclusion: The two AXL protocols are safe and appear to show comparable efficacy in disease stability. They can be used alternatively in the treatment of progressive keratoconus. [ABSTRACT FROM AUTHOR]

Background: Ultraviolet-A1 phototherapy has been used to treat many inflammatory dermatoses. Aims: To determine the efficacy and safety of ultraviolet-A1 phototherapy in Asian skin. Materials and Methods: We performed a review of records of patients undergoing ultraviolet-A1 phototherapy at our dermatology unit in Singapore from January 2007 to January 2011. Their electronic medical records were reviewed and a standardized questionnaire was filled up for data collection and tabulation. Chi-square or Fisher's exact tests were used to compare the difference in response between various groups for each characteristic. P value of < 0.05 was considered statistically significant. Results: Our study comprised of 159 patients, of which 103 were patients with hand and foot eczema, 21 with atopic dermatitis, 17 with scleroderma and the remaining with miscellaneous dermatoses. Of these patients, 47.6% of patients with hand and feet eczema had good response after 10 sessions, which increased to 75% after 20 sessions and to 84.6% after 30 sessions. After 10 sessions, 47.6% of patients with atopic dermatitis had good response, which increased to 66.7% after 20 sessions. After 30 sessions, all the three remaining patients with atopic dermatitis experienced good response. For patients with scleroderma, only 11.8 and 10% had good response after 10 and 20 sessions, respectively, which increased to 40% after 30 sessions. Limitations: Limitations of our study include its retrospective design and, consequently, the lack of standardized treatment protocol, as well as subjective assessment in terms of clinical improvement. Conclusions: Ultraviolet-A1 phototherapy appears to be efficacious for the treatment of hand and foot eczema as well as atopic dermatitis. However, in patients with scleroderma, the response was partial and needed a longer duration of treatment. [ABSTRACT FROM AUTHOR]

Nadyr A Damasceno,1 Nadia C Miguel,2 Marcelo Palis Ventura,3 Miguel Burnier Jr,4 Marcos P Avila,5 Eduardo F Damasceno3 1Ophthalmology Department, Hospital Naval Marcílio Dias, 2Laboratory of Neurohistology and Cell Ultrastructure, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 3Ophthalmology Department, Universidade Federal Fluminense, Niterói, Brazil; 4Ophthalmology Department, McGill University, Montreal, QC, Canada; 5Ophthalmology Department, Universidade Federal de Goiás, Goiania, Brazil Purpose: The aim of study was to evaluate the cross-link using riboflavin and ultraviolet A (UVA) for improving scleral wound healing.Materials and methods: This was an experimental study involving four New Zealand rabbits (eight eyes). Therapy procedure was chosen for the right eye and control procedure for the left one. UVA irradiation of 365 nm with a surface irradiance of 3 mW/cm2 and a photosensitizer of riboflavin drops were applied for 30 minutes on the right eye at 2 mm from the limbus. Sclerotomy incision was performed at 2 mm from the limbus in both right (on the cross-link-treated area) and left eye. Then, 30 days after surgery, a morphological analysis and histological staining with hematoxylin–eosin and picrosirius red were performed, and the sclerotomy cicatrization of right and left eyes was compared. The variables investigated were as follows: sclerotomy incision pictures and measurements were made using the ImageJ Software. Scleral thickness was measured (employing the anterior optical coherence tomography and the digital caliper). Collagen fiber density stained with picrosirius red staining was measured using the Image Pro Plus software.Results: The morphological analysis showed that in all samples, the right eye presented sclerotomy closure, and in two eyes, among them, there were no visible edges of the sclerotomies incision. The left eye presented sclerotomy closure and incision edges. The Image Pro Plus demonstrated a higher density of collagen fibers in the right eye when compared to the left one. The statistical analysis was significant when compared to the collagen fiber density in the treated eyes with the control eyes.Conclusion: The cross-link procedure resulted in a better sclerotomy wound healing. Keywords: sclerotomy wound healing, cross-linking, ultraviolet A, riboflavin

Pinitol (3-O-Methyl-D-chiro-inositol) has been reported to possess insulin-like effects and is known as one of the anti-diabetic agents to improve muscle, liver, and endothelial cells. However, the beneficial effects of pinitol on the skin are not well known. Here, we investigated whether pinitol had effects on human dermal fibroblasts (HDFs), and human dermal equivalents (HDEs) irradiated with ultraviolet A (UVA), which causes various damages including photodamage in the skin. We observed that pinitol enhanced wound healing in UVA-damaged HDFs. We also found that pinitol significantly antagonized the UVA-induced up-regulation of matrix metalloproteinase 1 (MMP1), and the UVA-induced down-regulation of collagen type I and tissue inhibitor of metalloproteinases 1 (TIMP1) in HDEs. Electron microscopy analysis also revealed that pinitol remarkably increased the number of collagen fibrils with regular banding patterns in the dermis of UVA-irradiated human skin equivalents. Pinitol significantly reversed the UVA-induced phosphorylation levels of ERK and JNK but not p38, suggesting that this regulation may be the mechanism underlying the pinitol-mediated effects on UVA-irradiated HDEs. We also observed that pinitol specifically increased Smad3 phosphorylation, which is representative of the TGF-β signaling pathway for collagen synthesis. These data suggest that pinitol exerts several beneficial effects on UVA-induced damaged skin and can be used as a therapeutic agent to improve skin-related diseases.

This study was to investigate the effects of metformin (MF) on ultraviolet A (UVA)-induced expression of matrix metalloproteinases (MMPs) and type I collagen (COL-I) in human skin fibroblasts (HSFs). HSFs were cultured in vitro and divided into control group, UVA group, and UVA + MF group. Cell proliferation was detected by CCK-8 method, and intracellular reactive oxygen species (ROS) level was detected by flow cytometry with fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCF-DA) staining. Meanwhile, real-time polymerase chain reaction (PCR) was used to examine the relative messenger RNA (mRNA) expression of aging-related genes, including MMP1, MMP3, and COL-I. Moreover, the expression of MMP1, MMP3, and COL-I proteins was further detected by western blot. Compared with the control group, the ROS content in the UVA group was increased significantly (P < 0.05), while the ROS content in the UVA + MF group was evidently lower than that in the UVA group (P < 0.05). In addition, the MMP1 and MMP3 mRNA levels were significantly elevated, while the COL-I mRNA was significantly decreased in UVA-induced HSF cells compared with the control cells. However, MF could significantly inhibit the improved MMP1 and MMP3 mRNA level and increase the COL-I mRNA level. Moreover, MF could significantly reverse the increasing MMP1 and MMP3 protein level and decreasing COL-I protein level induced by UVA. In conclusion, MF can increase the antioxidant capacity of cells and increase the synthesis of COL-I by inhibiting the level of intracellular ROS and the expression of related MMPs, thereby inhibiting the UVA-induced photoaging effect of HSF. [ABSTRACT FROM AUTHOR]

Exposure to excess ultraviolet (UV) A radiation induces the degradation/modification of both eumelanin and pheomelanin that may be deleterious to pigmented tissues. Although the spectral distribution of solar energy comprises nearly half of visible light (VL), few studies have been conducted to examine the role of VL in the photodegradation of both types of melanin, either VL alone or in combination with UVA. In this study, we examined the effects of physiological doses of VL (150 to 300 J cm−2) alone or in combination with a physiological dose of UVA (20 J cm−2) in normal human epidermal melanocytes. The degradation/modification of melanin structures was evaluated by our chemical degradation—high performance liquid chromatography methods. The results show that VL accelerates UVA‐induced changes in the structural features of both eumelanin and pheomelanin, although VL or UVA alone induced only minor changes in melanin structure. The differential spectral method provides support for the additive effects of VL. [ABSTRACT FROM AUTHOR]

Many moisturisers contain sun protection factors (SPF) equivalent to those found in sunscreens. However, there is a lack of research into how SPF moisturiser application compares to sunscreens in terms of coverage achieved and protection afforded. Previously we demonstrated that users incompletely covered their eyelid regions during routine sunscreen application. Here, we aimed to determine if SPF moisturiser users also displayed these tendencies. A study population of 84 participants (22 males, 62 females, age 18–57) were exposed to UV radiation and photographed using a tripod mounted UV sensitive DSLR camera on two separate visits. At visit one, images were acquired before and after applying either SPF30 sunscreen or moisturiser, then at visit two the study was repeated with the other formulation. Images were processed for facial landmark identification followed by segmentation mapping of hue saturation values to identify areas of the face that were/were not covered. Analyses revealed that application of moisturiser was significantly worse than sunscreen in terms area of the whole face missed (11.1% missed with sunscreen compared to 16.6% for SPF moisturiser p<0.001 paired t-test). This difference was primarily due to decreased coverage of the eyelid regions (14.0% missed with sunscreen, 20.9% moisturiser, p<0.001). Analysis of a post-study questionnaire revealed participants to be unaware of their incomplete coverage. Secondary analyses revealed improved coverage in males (p = 0.05), and, with moisturiser only, in participants with darker skin tones (p = 0.02). Together these data indicate that, despite potential advantages in terms of increased frequency of application of moisturiser, the areas of the face that are at higher cancer risk are likely not being protected, and that participants are unaware that they are at risk. As such, alternative sun-protection strategies should be promoted. [ABSTRACT FROM AUTHOR]

Extracorporeal photopheresis (ECP) is an autologous immunomodulatory cell therapy that consists of the ex vivo collection of mononuclear cells (MNCs), which are irradiated with UVA in the presence of the photosensitizing agent 8-methoxypsoralen (8-MOP) to induce cell apoptosis. This photoactivated cell preparation is then reinfused into the patient. While the clinical benefits of ECP are well-demonstrated, no study has yet characterized the influence of variations in the composition of the cell preparation on the efficacy of ECP in vitro. Here, we describe a standardized methodology for the in vitro assessment of ECP that uses the human lymphoma T-cell line and mimics the clinical procedure. By quantifying cell apoptosis, inhibition of cell proliferation, and 8-MOP consumption, we used this approach to characterize the specific influence of key variables on the cellular response to ECP. We found that (i) increases in hematocrit and plasma concentrations attenuated the cellular response to ECP; (ii) plasma concentration was the only variable tested that influenced 8-MOP consumption; and (iii) the loss of efficacy due to variations in the concentration of certain blood components could be counteracted by modulating the UVA dose. This methodology may enable evaluation of other leukapheresis preparation protocols and better determination of the optimal working parameters for ECP. [ABSTRACT FROM AUTHOR]

Ultraviolet A (UVA) radiation is harmful for living organisms but in low doses may stimulate cell proliferation. Our aim was to examine the relationships between exposure to different low UVA doses, cellular proliferation, and changes in cellular reactive oxygen species levels. In human colon cancer (HCT116) and melanoma (Me45) cells exposed to UVA doses comparable to environmental, the highest doses (30–50 kJ/m2) reduced clonogenic potential but some lower doses (1 and 10 kJ/m2) induced proliferation. This effect was cell type and dose specific. In both cell lines the levels of reactive oxygen species and nitric oxide fluctuated with dynamics which were influenced differently by UVA; in Me45 cells decreased proliferation accompanied the changes in the dynamics of H2O2 while in HCT116 cells those of superoxide. Genes coding for proteins engaged in redox systems were expressed differently in each cell line; transcripts for thioredoxin, peroxiredoxin and glutathione peroxidase showed higher expression in HCT116 cells whereas those for glutathione transferases and copper chaperone were more abundant in Me45 cells. We conclude that these two cell types utilize different pathways for regulating their redox status. Many mechanisms engaged in maintaining cellular redox balance have been described. Here we show that the different cellular responses to a stimulus such as a specific dose of UVA may be consequences of the use of different redox control pathways. Assays of superoxide and hydrogen peroxide level changes after exposure to UVA may clarify mechanisms of cellular redox regulation and help in understanding responses to stressing factors. [ABSTRACT FROM AUTHOR]

Background and Objective: Currently, photocatalysts have become a major focus of research in physics, chemistry, and surface engineering. It is hoped that this science help to solve various environmental problems. This study investigated the effect of ultraviolet lamp type on the removal efficiency of xylene utilizing the fixation of TiO2 nanoparticles on the activated carbon absorber and passing different concentrations of polluted air through xylene. Materials and Methods: In this experimental study, after the fixation of nanoparticles on activated carbon, the produced catalyst characteristics with the specific surface area were determined using Brunauer-Emmett-Teller (BET) analysis and scanning electron microscope (SEM). A dynamic concentrator system was used in order to produce xylene vapor. Moreover, the photocatalytic removal efficiency of xylene with three inlet concentrations of the pollutant was studied under ultraviolet rays in the wavelengths of A and C regions. Results: The results of BET analysis and SEM images showed that nanoparticles were well fixed on the surface of activated carbon. Moreover, the photocatalytic removal efficiency in the concentrations of 50, 100 and 300 ppm was equal to 87.8%, 98.9%, and 90.8%, respectively. In addition, no significant difference was observed between ultraviolet A and C regarding photocatalytic removal efficiency of xylene. Conclusion: According to the results of this study, the inlet concentration of the pollutant had an effect on its photocatalytic removal efficiency. However, there was no significant difference between ultraviolet A and C regarding photocatalytic removal efficiency of xylene. [ABSTRACT FROM AUTHOR]

Cannabidiol (CBD), a natural occurring phytocannabinoid, is used extensively in consumer products ranging from foods to shampoos, topical oils and lotions. Several studies demonstrated the anti‐inflammatory and antioxidative properties of cannabidiol. Nevertheless, the role of cannabidiol use in sunscreens is largely unknown as no studies on its effect on keratinocytes or melanocytes exist. As such, we aimed to explore the effect of CBD on keratinocyte and melanocyte viability following ultraviolet B (UVB) irradiation. CBD exhibited a dose‐dependent protective effect on both keratinocytes and melanocyte viability. Further, since CBD does not demonstrate absorption in the UVB spectra, we speculate that the protective effect is due to reduction in reactive oxygen species. To our knowledge, this is the first study demonstrating the protective effect of CBD on keratinocytes and melanocytes irradiated with UVB. [ABSTRACT FROM AUTHOR]

Leopoldo Spadea, Emanuele Tonti, Enzo Maria Vingolo Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Latina, Italy Abstract: Collagen cross-linking (CXL) is a relatively new conservative approach for progressive corneal ectasia, which is able to strengthen corneal tissue reforming new covalent bonds. Subjective and objective results following this method seem to be promising. In recent years, newer CXL protocols have been developed to perform more effective and less invasive procedures. The increasing diffusion of CXL in the corneal ectatic disease has increased the need to have actual indices regarding the efficacy of the treatment. Evaluation of demarcation line (DL), a transition zone between the cross-linked anterior corneal stroma and the untreated posterior corneal stroma, is considered a measurement of the depth of CXL treatment into the stroma. Some evidence in the literature emphasize that DL could be a measure of effectiveness of the CXL. On the contrary, some authors believe that the “the deeper, the better” principle is rather a simplistic approach for interpreting the clinical importance of the corneal stromal DL. Keywords: corneal cross-linking, demarcation line, keratoconus, ultraviolet A