1. Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels
- Author
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Peter-Maria Vogt, Birgit Andrée, Axel Haverich, Stefan Kalies, Houda Ichanti, Andres Hilfiker, Sarah Strauß, and Alexander Heisterkamp
- Subjects
0301 basic medicine ,Stromal cell ,Endothelium ,lcsh:Medicine ,Article ,Collagen Type I ,Culture Media, Serum-Free ,03 medical and health sciences ,0302 clinical medicine ,Tissue engineering ,human adipose tissue derived stromal cells (hASCs) ,Human Umbilical Vein Endothelial Cells ,medicine ,Humans ,ddc:530 ,ddc:610 ,human umbilical vein endothelial cells (HUVECs) ,3D endothelial cell networks ,lcsh:Science ,Matrigel ,Multidisciplinary ,Decellularization ,Chemistry ,lcsh:R ,Hydrogels ,tubular endothelial cell networks ,Coculture Techniques ,Cell biology ,Endothelial stem cell ,030104 developmental biology ,medicine.anatomical_structure ,Adipose Tissue ,Cell culture ,3D networks ,Self-healing hydrogels ,lcsh:Q ,Endothelium, Vascular ,Dewey Decimal Classification::500 | Naturwissenschaften::530 | Physik ,Stromal Cells ,Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit ,030217 neurology & neurosurgery - Abstract
Implementation of tubular endothelial cell networks is a prerequisite for 3D tissue engineering of constructs with clinically relevant size as nourishment of cells is challenged by the diffusion limit. In vitro generation of 3D networks is often achieved under conditions using serum containing cell culture medium and/or animal derived matrices. Here, 3D endothelial cell networks were generated by using human umbilical vein endothelial cells (HUVECs) in combination with human adipose tissue derived stromal cells (hASCs) employing human collagen I as hydrogel and decellularized porcine small intestinal submucosa as starter matrix. Matrigel/rat tail collagen I hydrogel was used as control. Resulting constructs were cultivated either in serum-free medium or in endothelial growth medium-2 serving as control. Endothelial cell networks were quantified, tested for lumen formation, and interaction of HUVECs and hASCs. Tube diameter was slightly larger in constructs containing human collagen I compared to Matrigel/rat tail collagen I constructs under serum-free conditions. All other network parameters were mostly similar. Thereby, the feasibility of generating 3D endothelial cell networks under serum-free culture conditions in human collagen I as hydrogel was demonstrated. In summary, the presented achievements pave the way for the generation of clinical applicable constructs.
- Published
- 2019