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Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019), Scientific Reports (2019), Nr. 9, Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Implementation of tubular endothelial cell networks is a prerequisite for 3D tissue engineering of constructs with clinically relevant size as nourishment of cells is challenged by the diffusion limit. In vitro generation of 3D networks is often achieved under conditions using serum containing cell culture medium and/or animal derived matrices. Here, 3D endothelial cell networks were generated by using human umbilical vein endothelial cells (HUVECs) in combination with human adipose tissue derived stromal cells (hASCs) employing human collagen I as hydrogel and decellularized porcine small intestinal submucosa as starter matrix. Matrigel/rat tail collagen I hydrogel was used as control. Resulting constructs were cultivated either in serum-free medium or in endothelial growth medium-2 serving as control. Endothelial cell networks were quantified, tested for lumen formation, and interaction of HUVECs and hASCs. Tube diameter was slightly larger in constructs containing human collagen I compared to Matrigel/rat tail collagen I constructs under serum-free conditions. All other network parameters were mostly similar. Thereby, the feasibility of generating 3D endothelial cell networks under serum-free culture conditions in human collagen I as hydrogel was demonstrated. In summary, the presented achievements pave the way for the generation of clinical applicable constructs.
- Subjects :
- 0301 basic medicine
Stromal cell
Endothelium
lcsh:Medicine
Article
Collagen Type I
Culture Media, Serum-Free
03 medical and health sciences
0302 clinical medicine
Tissue engineering
human adipose tissue derived stromal cells (hASCs)
Human Umbilical Vein Endothelial Cells
medicine
Humans
ddc:530
ddc:610
human umbilical vein endothelial cells (HUVECs)
3D endothelial cell networks
lcsh:Science
Matrigel
Multidisciplinary
Decellularization
Chemistry
lcsh:R
Hydrogels
tubular endothelial cell networks
Coculture Techniques
Cell biology
Endothelial stem cell
030104 developmental biology
medicine.anatomical_structure
Adipose Tissue
Cell culture
3D networks
Self-healing hydrogels
lcsh:Q
Endothelium, Vascular
Dewey Decimal Classification::500 | Naturwissenschaften::530 | Physik
Stromal Cells
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....98c016eec1704d04c8aae16bcf016bdd