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2 results on '"raw camel meat"'

1. Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat

Author

Khaloud Mohammed Alarjani, Amani H. Aljahani, Hany M. Yehia, and Manal F. El-Khadragy

Subjects
DNA, Bacterial, 0301 basic medicine, Camelus, Meat, medicine.drug_class, Bacterial Toxins, 030106 microbiology, Antibiotics, raw camel meat, Biophysics, Microbial Sensitivity Tests, medicine.disease_cause, Biochemistry, Molecular Bases of Health & Disease, law.invention, Microbiology, Foodborne Diseases, Hemolysin Proteins, 03 medical and health sciences, 0404 agricultural biotechnology, Antibiotic resistance, Listeria monocytogenes, law, Drug Resistance, Multiple, Bacterial, medicine, Animals, Listeriosis, antimicrobial resistance, Molecular Biology, Gene, Heat-Shock Proteins, Research Articles, Polymerase chain reaction, biology, Strain (chemistry), Listeriolysin O, 04 agricultural and veterinary sciences, Cell Biology, biology.organism_classification, 040401 food science, Anti-Bacterial Agents, Food Microbiology, Listeria, listeriolysin O (hly) gene, Neuroscience
Abstract

In the present study, a total of 50 raw camel meat samples were analyzed for the presence of Listeria monocytogenes. The isolates were characterized via morphological and culture analyses; identification of isolates was confirmed by polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The API Listeria system was used for further chemical identification and verification of the strains. L. monocytogenes was identified in eight raw camel meat samples, which was the highest incidence (16%) of contamination, followed by L. seeligeri 3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were positive for the listeriolysin O gene were >99% similar to the published database sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We studied the antibiotic resistance profile of the L. monocytogenes strains with common antibiotics used to treat human listeriosis and demonstrated that almost all strains tested were susceptible to the antibiotics.

Published
2020

2. Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat.

Author

Yehia HM, Elkhadragy MF, Aljahani AH, and Alarjani KM

Subjects
Animals, Bacterial Toxins genetics, DNA, Bacterial genetics, Foodborne Diseases microbiology, Heat-Shock Proteins genetics, Hemolysin Proteins genetics, Listeria monocytogenes genetics, Listeria monocytogenes isolation & purification, Listeriosis microbiology, Listeriosis transmission, Microbial Sensitivity Tests, Anti-Bacterial Agents pharmacology, Camelus microbiology, Drug Resistance, Multiple, Bacterial, Food Microbiology, Foodborne Diseases prevention & control, Listeria monocytogenes drug effects, Listeriosis prevention & control, Meat microbiology
Abstract

In the present study, a total of 50 raw camel meat samples were analyzed for the presence of Listeria monocytogenes. The isolates were characterized via morphological and culture analyses; identification of isolates was confirmed by polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The API Listeria system was used for further chemical identification and verification of the strains. L. monocytogenes was identified in eight raw camel meat samples, which was the highest incidence (16%) of contamination, followed by L. seeligeri 3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were positive for the listeriolysin O gene were >99% similar to the published database sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We studied the antibiotic resistance profile of the L. monocytogenes strains with common antibiotics used to treat human listeriosis and demonstrated that almost all strains tested were susceptible to the antibiotics., (© 2020 The Author(s).)

Published
2020
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