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Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat
- Source :
- Bioscience Reports
- Publication Year :
- 2020
- Publisher :
- Portland Press Ltd., 2020.
-
Abstract
- In the present study, a total of 50 raw camel meat samples were analyzed for the presence of Listeria monocytogenes. The isolates were characterized via morphological and culture analyses; identification of isolates was confirmed by polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The API Listeria system was used for further chemical identification and verification of the strains. L. monocytogenes was identified in eight raw camel meat samples, which was the highest incidence (16%) of contamination, followed by L. seeligeri 3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were positive for the listeriolysin O gene were >99% similar to the published database sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We studied the antibiotic resistance profile of the L. monocytogenes strains with common antibiotics used to treat human listeriosis and demonstrated that almost all strains tested were susceptible to the antibiotics.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Camelus
Meat
medicine.drug_class
Bacterial Toxins
030106 microbiology
Antibiotics
raw camel meat
Biophysics
Microbial Sensitivity Tests
medicine.disease_cause
Biochemistry
Molecular Bases of Health & Disease
law.invention
Microbiology
Foodborne Diseases
Hemolysin Proteins
03 medical and health sciences
0404 agricultural biotechnology
Antibiotic resistance
Listeria monocytogenes
law
Drug Resistance, Multiple, Bacterial
medicine
Animals
Listeriosis
antimicrobial resistance
Molecular Biology
Gene
Heat-Shock Proteins
Research Articles
Polymerase chain reaction
biology
Strain (chemistry)
Listeriolysin O
04 agricultural and veterinary sciences
Cell Biology
biology.organism_classification
040401 food science
Anti-Bacterial Agents
Food Microbiology
Listeria
listeriolysin O (hly) gene
Neuroscience
Subjects
Details
- ISSN :
- 15734935 and 01448463
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....4fbb272b26743668ddeba2b8fd98d02a