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The process conditions of ultrasonic-assisted ethanol extraction of resveratrol in peanut roots and the purification of resveratrol by macroporous resin were studied. The effects of ethanol concentration, ultrasonic power, ultrasonic time and solid-liquid ratio on the extraction rate of resveratrol from peanut root were investigated. After optimization by single-factor experiments and response surface methodology, a promised yield of resveratrol (175.53±1.57 μg/g) was obtained under optimal conditions: Ethanol concentration 90%, ultrasonic power 290 W, ultrasonic time 12 min, and solid-liquid ratio 1:38 (g:mL). Analysis of variance was performed to find that the effects of ultrasonic time, ultrasonic power and ethanol concentration on the extracted content of resveratrol reached the significance level (P

Peanut (Arachis hypogaea L.) is an important oil crop globally because of its high edible and economic value. However, its yield and quality are often restricted by certain soil factors, especially nitrogen (N) deficiency, and soil compaction. To explore the molecular mechanisms and metabolic basis behind the peanut response to N deficiency and soil compaction stresses, transcriptome and metabolome analyses of peanut root were carried out. The results showed that N deficiency and soil compaction stresses clearly impaired the growth and development of peanut's aboveground and underground parts, as well as its root nodulation. A total of 18645 differentially expressed genes (DEGs) and 875 known differentially accumulated metabolites (DAMs) were identified in peanut root under differing soil compaction and N conditions. The transcriptome analysis revealed that DEGs related to N deficiency were mainly enriched in "amino acid metabolism", "starch and sucrosemetabolism", and "TCA cycle" pathways, while DEGs related to soil compaction were mainly enriched in "oxidoreductase activity", "lipids metabolism", and "isoflavonoid biosynthesis" pathways. The metabolome analysis also showed significant differences in the accumulation of metabolisms in these pathways under different stress conditions. Then the involvement of genes and metabolites in pathways of "amino acid metabolism", "TCA cycle", "lipids metabolism", and "isoflavonoid biosynthesis" under different soil compaction and N deficiency stresses were well discussed. This integrated transcriptome and metabolome analysis study enhances our mechanistic knowledge of how peanut plants respond to N deficiency and soil compaction stresses. Moreover, it provides new leads to further investigate candidate functional genes and metabolic pathways for use in improving the adaptability of peanut to abiotic stress and accelerating its breeding process of new stress-resistant varieties. [ABSTRACT FROM AUTHOR]

In this study, peanut root was used as the main test material, ethanol solution was used as the extraction solvent, ultrasonic-assisted extraction of free polyphenols in peanut roots, and high performance liquid chromatography (HPLC) analysis of peanut roots The composition and content distribution of phenol. Detection conditions: mobile phase: A is methanol, B is 0.5% formic acid, using a gradient elution procedure. The results of the study showed that the optimal process conditions for ultrasonic-assisted extraction of polyphenols from peanut roots were ethanol volume fraction 70%, material-to-liquid ratio 1:30 (g/mL), extraction temperature 45°C, ultrasonic time 50 min, to obtain peanut roots The optimal extraction amount of polyphenols is 1.629 mg/g. The main polyphenols in peanut roots were detected by high performance liquid chromatography as tea polyphenols, resveratrol, p-coumaric acid and caffeic acid. [ABSTRACT FROM AUTHOR]

Peanut ( Arachis hypogaea L.) is an important oil crop globally because of its high edible and economic value. However, its yield and quality are often restricted by certain soil factors, especially nitrogen (N) deficiency, and soil compaction. To explore the molecular mechanisms and metabolic basis behind the peanut response to N deficiency and soil compaction stresses, transcriptome and metabolome analyses of peanut root were carried out. The results showed that N deficiency and soil compaction stresses clearly impaired the growth and development of peanut's aboveground and underground parts, as well as its root nodulation. A total of 18645 differentially expressed genes (DEGs) and 875 known differentially accumulated metabolites (DAMs) were identified in peanut root under differing soil compaction and N conditions. The transcriptome analysis revealed that DEGs related to N deficiency were mainly enriched in "amino acid metabolism", "starch and sucrose metabolism", and "TCA cycle" pathways, while DEGs related to soil compaction were mainly enriched in "oxidoreductase activity", "lipids metabolism", and "isoflavonoid biosynthesis" pathways. The metabolome analysis also showed significant differences in the accumulation of metabolisms in these pathways under different stress conditions. Then the involvement of genes and metabolites in pathways of "amino acid metabolism", "TCA cycle", "lipids metabolism", and "isoflavonoid biosynthesis" under different soil compaction and N deficiency stresses were well discussed. This integrated transcriptome and metabolome analysis study enhances our mechanistic knowledge of how peanut plants respond to N deficiency and soil compaction stresses. Moreover, it provides new leads to further investigate candidate functional genes and metabolic pathways for use in improving the adaptability of peanut to abiotic stress and accelerating its breeding process of new stress-resistant varieties., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Yang, Wu, Liang, Yin and Shen.)