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Background: Stunting causes growth and development issues which are characterized by a systemic inflammatory response and neurotransmitter disorder. The South Kalimantan natural resources i.e. extracts of Pasak Bumi (Eurycoma longifolia Jack) and seluang fish have the potential as a modified therapy for stunting management. Objective: To analyze the effect of Pasak Bumi and seluang fish administration in overcoming systemic inflammation and neurotransmitter disorders in the stunting rats model. Methods: Rats were divided into six group i.e Positive Control (KP): Stunting rats + placebo + standard feed; (P1): stunting rats + 70% EPB 15 mg/kg body weight (BW) + standard feed; (P2): Stunting rats + DHA 1 mg/kg BW + standard feed; (P3): Stunting rats + EPB 15 mg/kg BW + DHA 1 mg/kg BW + standard feed; (P4): Stunting rats + seluang fish; (P5): Stunting rats + EPB 15 mg/kg BW + seluang fish for 5 weeks; Parameters measured were levels of IL-6, TNF-α, and serotonin. Results: There were significant differences in levels of TNF-α (p = 0.000) and serotonin (p = 0.000). Conclusion: The administration of Pasak Bumi extract and seluang fish was able to inhibit systemic inflammation and neurotransmitter dysregulation.

The research was used to produce functional instant green coffee through gambier catechin extract and pasak bumi powder. This involved using a non-factorial completely randomized design with 5 treatments and 3 replications. The treatments consist of 5 formulations (F), including the instant green coffee (%), gambir catechin extract (%), and pasak bumi powder (%) where F1 was at 100:0:0, F2 was 80:15:5, F3 was 70:20:10, F4 was 60:25:15, and F5 was 50:30:20. The results showed the functional instant green coffee produced has a water content of 3.84 – 4.81%, soluble speed of 26.78 – 29.33 seconds, and total phenol of 16.79 – 169.48 mg/L, and IC50 of 44.68- 207.59 ppm. The addition of gambier catechin extract and pasak bumi powder to the formulation was observed to have significantly increased the functional properties and water content. Moreover, the soluble speed of the instant coffee fulfils the quality requirements of the Indonesian National Standard (SNI) number 2983 of 2014.

The research was used to produce functional instant green coffee through gambier catechin extract and pasak bumi powder. This involved using a non-factorial completely randomized design with 5 treatments and 3 replications. The treatments consist of 5 formulations (F), including the instant green coffee (%), gambir catechin extract (%), and pasak bumi powder (%) where F1 was at 100:0:0, F2 was 80:15:5, F3 was 70:20:10, F4 was 60:25:15, and F5 was 50:30:20. The results showed the functional instant green coffee produced has a water content of 3.84 - 4.81%, soluble speed of 26.78 - 29.33 seconds, and total phenol of 16.79 - 169.48 mg/L, and IC50 of 44.68 - 207.59 ppm. The addition of gambier catechin extract and pasak bumi powder to the formulation was observed to have significantly increased the functional properties and water content. Moreover, the soluble speed of the instant coffee fulfils the quality requirements of the Indonesian National Standard (SNI) number 2983 of 2014. [ABSTRACT FROM AUTHOR]

The use of synthetic steroids of 17-Methytestosterone (MT) to produce a monosexual population of male Tilapia (Oreochromis niloticus) has been restricted because it can leave harmful residues in fish and the environment. One alternative that can be done is by using natural bioactive derived from plants with androgenic properties, such as Pasak Bumi (Eurycoma longifolia Jack). The aim of this research was to assay the use of methanol extract from Pasak bumi roots with immersion techniques for masculinization of tilapia. The research procedure was carried out with three treatment doses of methanol extract from the Pasak Bumi roots (namely 30, 60, and 90 mg/L), treatment dose amounted to 0 mg/L acted as a negative control and treatment of MT amounted to 50 mg/L acted as a positive control, with three replications on each treatment. Immersion was applied to larvae that was hatched out of egg yolk (seven day-old) for 24 hours and was maintained with a capacity of 50 larvae per aquarium during 60 days. The results showed that the treatment of the methanol extract of Pasak Bumi roots gave male sex ratios ranging from 72.66% - 76.33% higher than the negative control which showed the results of male sex ratios amounted to 49.19% and lower than the positive control of MT which the results amounted to 83.99%. The range of survival rate was from 80.67% - 85.33%, while the range of survival rate in the negative control was 83.33% and in positive control of MT was 82.67%. Thus, the methanol extract of Pasak Bumi roots can be used for masculinization of tilapia by immersion method with the best performance at a dose of 60 mg/L – 90 mg/L.

Pasak Bumi is a local name for a medicinal plant in Kalimantan, Indonesia. It is a famous medicinal plant and commonly used in traditional medicine as an aphrodisiac, as well as in the treatment of malaria. Pasak Bumi is a commercial name for Eurycoma longifolia (Simaroubaceae) plant species. Besides Eurycoma longifolia there are two other plant species also known locally as Pasak Bumi, Rennelia elliptica (Rubiaceae) and Trivalvaria macrophylla (Annonaceae). This study was performed to investigate the antimicrobial activities of the different species of Pasak Bumi and its total phenol contents. The antimicrobial activity of the ethanol extract was determined using the Agar Well Diffusion method at various concentrations while the phenol content was determined by the Folin - Ciocalteu method. The results of the ethanol extract from the different root showed that the T. macrophylla had the highest phenol content, and the highest activity index (AI) was found in the E. longifolia (0.96 at 1000 µg concentration). The results of this study show that the three different Pasak Bumi have potential as antimicrobials against oral pathogen; 1 yeast: Candida albicans, and 3 bacterias: Staphylococcus aureus, Streptococcus mutans, and Streptococcus sobrinus.

Tumbuhan obat dari hutan dapat tertukar secara tidak sengaja dengan jenis lain yang serupa tetapi kandungan bahan aktifnya tidak memadai atau bahan aktifnya sama sekali lain. Contohnya adalah pasak burni (Eurycoma longifalia Jack.- Simaroubaceae) yang juga dikenal dengan nama bidara laut. Nama bidara laut ini digunakan juga untuk jenis kayu lain dari suku Loganiaceae yaitu Strychnos ligustrinum Bl. Penelitian ini berrujuan unruk mengungkap karakteristik anatorni kayu Eurycoma longifolia dan perbedaannya dengan Strychnos ligustrinum. Selain itu dibandingkan juga ciri anatorni kayu Eurycoma longifalia dengan ciri anatorni kayu jenis kayu lain yang sesuku. Contoh kayu pasak burni dikumpulkan dari Kuok, Riau, dan Muara Tebo, Jambi. Jenis lainnya diperoleh dari koleksi kayu autentik Puslitbang Hasil Hutan. Contoh kayu terlebih dahulu dibuat preparat sayat lalu diamati strukrur anatominya. Dimensi pembuluh dans erat diamati dari preparat maserasi. Hasil yang diperoleh menunjukkan bahwa kayu Eurycoma longifolia dapat dikenali dan dibedakan dari Strychnos ligustrinum terutama berdasarkan diameter dan sebaran pembuluh serta dari kehadiran kulit tersisip. Kayu Strichnos ligustrinum mempunyai pembuluh berdiameter jauh lebih kecil dengan sebaran dendritik serta mengandung kulit tersisip di antara jaringan kayu. Ciri demikian tidak dimiliki kayu Eurycoma longifolia. Kayu Eurycoma longifolia dapat juga dibedakan dari jenis lain dari suku yang sama berdasarkan karaktersitik pembuluh, parenkim, jari-jari, serat, dan kehadiran silika dalam jari-jari kayu.

Pasak bumi (Eurycoma longifolia Jack) merupakan salah satu jenis tumbuhan hutan tropis di Indonesia yang pemanfaatannya sebagai bahan baku dalam pembuatan obat baik yang modern maupun tradisional. Pasak bumi mengandung senyawa erikomanon yang ampuh mengobati malaria dan senyawa kuasinoid serta alkaloid yang dapat menghambat pertumbuhan sel kanker. Pasak bumi juga mempunyai senyawa aktif flavonoid yang berfungsi untuk melindungi struktur sel, meningkatkan efektifitas vitamin C, anti inflamasi, mencegah keropos tulang, antioksidan, dan antibiotik. Penelitian ini bertujuan untuk membuat produk permen dan jelli dari bagian akar, batang, daun, ranting, dan kulit pasak bumi sehingga dapat meningkatkan nilai tambah dan memudahkan akses bagi masyarakat luas untuk mendapatkan khasiat zat aktif yang dikandung tumbuhan tersebut dalam bentuk produk inovatif. Standard produk yang dihasilkan (permen dan jelli) mengacu kepada SNI 3547.1:2008 tentang kembang gula keras dan SNI No. 3547.2:2008 tentang kembang gula lunak. Berdasarkan hasil pengujian permen, semua parameter yaitu kadar air, abu, gula, sukrosa, Zn, Hg, Pb, As, Angka Lempeng Total, Coliform, Ecoli, Salmonella dan Kapang/Khamir memenuhi syarat, kecuali untuk parameter Cu dengan nilai antara 3,48–8,17% tidak memenuhi standar yang dipersyaratkan yaitu maksimal 2%. Sedangkan produk jelli sebagian besar memiliki kadar air bervariasi antara 18,20–39,45% tidak memenuhi standar yang dipersyaratkan maksimal 20%. Hasil dari penelitian ini dapat meningkatkan nilai tambah dan nilai jual dari tumbuhan pasak bumi berupa produk permen dan jelli yang memiliki cita rasa, keunggulan dan manfaat bagi kesehatan.ABSTRAKPasak bumi (Eurycoma longifolia Jack) merupakan salah satu jenis tumbuhan hutan tropis di Indonesia yang pemanfaatannya sebagai bahan baku dalam pembuatan obat baik yang modern maupun tradisional. Pasak bumi mengandung senyawa erikomanon yang ampuh mengobati malaria dan senyawa kuasinoid serta alkaloid yang dapat menghambat pertumbuhan sel kanker. Pasak bumi juga mempunyai senyawa aktif flavonoid yang berfungsi untuk melindungi struktur sel, meningkatkan efektifitas vitamin C, anti inflamasi, mencegah keropos tulang, antioksidan, dan antibiotik. Penelitian ini bertujuan untuk membuat produk permen dan jelli dari bagian akar, batang, daun, ranting, dan kulit pasak bumi sehingga dapat meningkatkan nilai tambah dan memudahkan akses bagi masyarakat luas untuk mendapatkan khasiat zat aktif yang dikandung tumbuhan tersebut dalam bentuk produk inovatif. Standard produk yang dihasilkan (permen dan jelli) mengacu kepada SNI 3547.1:2008 tentang kembang gula keras dan SNI No. 3547.2:2008 tentang kembang gula lunak. Berdasarkan hasil pengujian permen, semua parameter yaitu kadar air, abu, gula, sukrosa, Zn, Hg, Pb, As, Angka Lempeng Total, Coliform, Ecoli, Salmonella dan Kapang/Khamir memenuhi syarat, kecuali untuk parameter Cu dengan nilai antara 3,48–8,17% tidak memenuhi standar yang dipersyaratkan yaitu maksimal 2%. Sedangkan produk jelli sebagian besar memiliki kadar air bervariasi antara 18,20–39,45% tidak memenuhi standar yang dipersyaratkan maksimal 20%. Hasil dari penelitian ini dapat meningkatkan nilai tambah dan nilai jual dari tumbuhan pasak bumi berupa produk permen dan jelli yang memiliki cita rasa, keunggulan dan manfaat bagi kesehatan.

The effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia Jack was different to that found in the Murashige and Skoog (MS) medium. The highest amount of alkaloids, 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one, could be obtained from E. longifolia Jack cells cultured in modified MS liquid medium that containing macronutrients: 21.50 mM NH4NO3, 14.25 mM KNO3, 7.50 mM CaCl2·2H2O, 2.50 mM MgSO4·7H2O, 1.45 mM KH2PO4, while content of micronutrients was 0.233 mM FeNa-EDTA, 0.215 mM MnSO4·4H2O and without CuSO4·5H2O. Increased sucrose concentration to 4.00% (w/v) in modified MS liquid medium could increase total of two-alkaloid. The modification of macronutrients and micronutrients concentration based the optimum production of biomass was obtained MSBs medium that producing high biomass but also increasing the production of 9-hydroxycanthin-6-one. The modification of macronutrients or macronutrients and micronutrients based the optimum total of two-alkaloid was obtained MSC and MSD medium that producing low fresh weight but producing the high 9-hydroxycanthin-6-one.

Eurycoma longifolia Jack is a tall slender shrub-tree which is well-reputed among the natives of South East Asia for its potent aphrodisiac effect. Its root extracts have shown important biological activities such as antitumor, antimalarial, antibacterial, anti-diabetic, anti-hypertensive, Osteoprotective, and ergogenic which are mainly attributed to quassinoids. Commercially it is available in the form of drinks (along with other herbs), capsules, or loose powders. Based on available online databases it was realized that in spite of numerous reports on medicinal properties of E. longifolia, a review of recent developments regarding phytopharmacology, safety and toxicology, pharmacokinetics, and clinical applications was lacking. Therefore, this concise yet informative piece of work was prepared by pulling together trustworthy information from all the accessible published and unpublished scientific resources to serve as a reliable source of reference for future investigations. [ABSTRACT FROM AUTHOR]

<b>Background and Objective:</b> The use of the roots of the pasak bumi (<i>E. longifolia</i> Jack) to treat cancer has been studied widely, however, the scientific basis of these plants used as an anticancer drug is widely unknown. The purpose of this study was to examine the anticancer activity of ethyl acetate and non-ethyl acetate fractions of pasak bumi roots in Raji cells. <b>Materials and Methods:</b> The cytotoxicity test is using the direct cell count method with trypan blue staining. The growth inhibition is using doubling time analysis of Raji cells. Observation of the apoptotic events of Raji cells used ethidium bromide staining, while observing the expression of p53 protein in Raji cells was done by immunohistochemical staining. <b>Results:</b> The results of the cytotoxicity and doubling time test showed that the activity of the non-ethyl acetate fraction was greater than that of the roots of pasak bumi. The lower concentration of non-ethyl acetate fraction of pasak bumi roots was able to delay the multiplication time of Raji cells which was greater than that of ethyl acetate. The results of the cytotoxicity and doubling time test showed that the activity of the non-ethyl acetate fraction was greater than that of the roots of pasak bumi. <b>Conclusion:</b> It can be concluded that the ethyl acetate and non-ethyl acetate fractions of the roots of pasak bumi have cytotoxic and antiproliferative activity on Raji cells, however they cannot induce apoptosis in Raji cells. The death of Raji cells is through the mechanism of inhibiting Raji cell proliferation as evidenced by an increase in p53 protein expression.