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The study focused on section imaging and electron microscopic structure analysis of Candida albicans culture treated with oregano essential oil. In the study we used cultures of Candida albicans ATCC pre-treated with essential oil of oregano which were initially left for 72 h at the thermostat, in order to be inhibited by the essential oil of oregano. We took samples from this environment and treated them properly so that they could be viewed and analyzed with the transmission electron microscope., {"references":["1.\t[online] http://ammrf.org.au/.","2.\tIkoma H, Kudo T, Peng Y, Broxton M, Wetzstein G., Deep learning multi-shot 3D localization microscopy using hybrid optical-electronic computing, Opt Lett. 2021 Dec 15;46(24):6023-6026. doi: 10.1364/OL.441743.PMID: 34913909","3.\tFaruqi AR, Henderson R., Electronic detectors for electron microscopy. Curr Opin Struct Biol. 2007 Oct;17(5):549-55. doi: 10.1016/j.sbi.2007.08.014. Epub 2007 Oct 29.PMID: 17913494 Review.","4.\tBik L, van Doorn MBA, Biskup E, Ortner VK, Haedersdal M, Olesen UH.,, Electronic Pneumatic Injection-Assisted Dermal Drug Delivery Visualized by Ex Vivo Confocal Microscopy.Lasers Surg Med. 2021 Jan;53(1):141-147. doi: 10.1002/lsm.23279. Epub 2020 Jun 8.PMID: 32515075","5.\tKrainii PA, Ibishev KS.Urologiia. , Electronic microscopy assessment of immunological disorders in the secret of the prostate in patients with chronic recurrent bacterial prostatitis, 2021 Sep;(4):68-72.PMID: 34486277 Russian","6.\tChing-Roa VD, Olson EM, Ibrahim SF, Torres R, Giacomelli MG., Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers.Sci Rep. 2021 Mar 4;11(1):5248. doi: 10.1038/s41598-021-84522-0.PMID: 33664354","7.\tFardian-Melamed N, Eidelshtein G, Rotem D, Kotlyar A, Porath D., Temperature Dependence of the STM Morphology and Electronic Level Structure of Silver-Containing DNA, Small. 2020 Feb;16(5):e1905901. doi: 10.1002/smll.201905901. Epub 2019 Dec 29.PMID: 31885142","8.\tChoi E, Kim A, Son H, Pyo SG.J Nanosci Nanotechnol.,Applications of scanning probe-atomic force microscopy in nanobioelectronics.,2014 Jan;14(1):924-31. doi: 10.1166/jnn.2014.8759.PMID: 24730309 Review.","9.\tWang M, Li M, Jiang S, Gao J, Xi P.Micron. , Plasmonics meets super-resolution microscopy in biology., 2020 Oct;137:102916.doi:10.1016/j.micron.2020.102916. Epub 2020 Jul15.PMID: 32688264 Review.","10.\tMoroianu O-N, Popescu N-D, Roșoiu N, Experimental Study on Inhibitor Effects of Substances Applied in Differential Dilutions on Albicans Candidate Cultures, Academy of Romanian Scientists, Annals Series on Biological Sciences, 2018, 7, 2, 61-69.","11.\tMoroianu Olimpia-Nicoleta, Popescu Nelu-Doru, Urse Alina, Gurgaş Leonard& Rosoiu Natalia, Study of the action of some chemical and natural substances on Candida albicans cultures. Norvegian Journal of Development of the International Science, no 91, 2022, 34-39.https://doi.org/10.5281/zenodo.7032255.","12.\tMoroianu Olimpia-Nicoleta, Popescu Nelu-Doru, Urse Alina Raluca, Rosoiu Natalia, Study on the action of Natural Products on Candida Albicans Crops . International Journal of Biomedical Engineering and Clinical Science. Vol. 8, No. 3, 2022, pp. 27-32. doi: 10.11648/j.ijbecs.20220803.11.","13.\tNostro A, Papalia T., Antimicrobial Activity of carvacrol: current progress and future prospective, April 2012, vol.7, no.1, pp.28-35 (8).","14.\tGuarda A., Rubilar J.F., Miltz J., Galatto M.J, The antimicrobial activity of microencapsulated thymol and carvacrol, International Journal of Food Microbiology, March 2011, vol.146, no.2., pp.144-150."]}

Export Date: 15 September 2021 Correspondence Address: Fagerstedt, K.W.; Department of Pathology, Finland; email: klaus.wj.fagerstedt@helsinki.fi Mixed neuroendocrine-non-neuroendocrine neoplasms (MINEN) are rare tumors that consist of at least 30% of both neuroendocrine and non-neuroendocrine components. The data concerning the pathogenesis of MINEN suggest a monoclonal origin. We describe a spontaneously immortalized cell line derived from gastric MINEN called GNEN-1. Primary tumor consisted of components of high-grade neuroendocrine carcinoma and adenocarcinoma. The GNEN-1 cell line was initiated from metastatic tumor cells of peritoneal fluid and expresses a purely neuroendocrine phenotype. The GNEN-1 cell line grows as monolayers and has retained the neuroendocrine phenotype with positivity for chromogranin A in immunohistochemistry. Electron microscopy showed cytoplasmic dense core granules and axon hillocks. The karyotype revealed alterations typical of both adenocarcinoma and neuroendocrine carcinoma such as trisomy 7 and 8. GNEN-1 cells were also positive for stanniocalcin-1, a marker of poor prognosis in gastric carcinomas. Expression of several markers related to neuroendocrine tumors was found. There have been only a few studies on the pathogenesis of MINEN and management of the disease due to the rarity of this tumor type. Here we describe for the first time an immortalized cell line derived from mixed gastric NEN. The GNEN-1 line offers a tool for future research on gastric NEN. © 2021 The authors.

AIM: There are many trials concerning peripheral nerve damage causes and treatment options. Unfortunately, nerve damage is still a major problem regarding health, social and economic issues. On this study, we used vascular graft and human cord blood derived stem cells to find an alternative treatment solution to this problem. MATERIAL AND METHODS: We used 21 female Wistar rats on our study. They were anesthetized with ketamine and we studied right hind limbs. On Group 1, we did a full layer cut on the right sciatic nerve. On Group 2, we did a full layer cut on the right sciatic nerve, and we covered synthetic vascular graft on cut area. On Group 3, we did a full layer cut on right sciatic nerve, and we covered the area with stem cell applied vascular graft. RESULTS: At the end of postoperative 8. weeks, we performed EMG on the rats. When we compared healthy and degenerated areas as a result of EMG, we found significant amplitude differences between the groups on healthy areas whereas there was no significant difference on degenerated areas between the groups. Then we re-opened the operated area again to reveal the sciatic nerve cut area, and we performed electron microscope evaluation. On the stem cell group, we observed that both the axon and the myelin sheet prevented degeneration. CONCLUSION: This study is a first on using synthetic vascular graft and cord blood derived CD34+ cells in peripheral nerve degeneration. On the tissues that were examined with electron microscope, we observed that CD34+ cells prevented both axonal and myelin sheath degeneration. Nerve tissue showed similar results to the control group, and the damage was minimal. © 2018 Ali Yilmaz, Abdullah Topcu, Cagdas Erdogan, Levent Sinan Bir, Barbaros Sahin, Gulcin Abban, Erdal Coskun, Ayca Ozkul.

The lack of an established protocol for scanning electron microscopy (SEM) studies on stem cells differentiating into adipogenic lineage led us to develop a protocol for the preparation of differentiated adult bone marrow-derived mesenchymal stem cells (BMSC) for SEM. This protocol describes the procedure to maintain and preserve the structural organization of cellular components following differentiation, for morphological and physical characterization. The fixation of the differentiated cells was followed by dehydration using methanol, and vacuum desiccation before microscopy. The use of longer chain alcohols as dehydrating agents was avoided in our method to reduce the dissolution of lipid deposits in cells, thus allowing the maintenance of their structural integrity. The time period for the processing of samples was reduced by avoiding the osmium tetroxide postfixation and critical point drying. Thus, this protocol helps in determining the potential, fate, and degree of stem cell differentiation. This may be useful for SEM analysis of differentiated cells, especially those grown on various scaffolds. � 2011 Elsevier Inc. All rights reserved.

Trigonal bipyramidal olefin-platinum complexes of the title chiral diamine (mestien) have been synthetized and their stereochemistry has been investigated by H-1 and C-13 NMR spectroscopy. The molecular structure of the complex PtClMe ((E)-ClCH = CHCl) (mestien) has been determined by X-ray diffraction analysis. The coordinated nitrogen atoms display a single configuration, and a high enantioface selectivity is observed in the coordination of prochiral olefins having a moderate lateral bulk. The selectivity can be correlated to the conformation adopted by the diamine ligand, which shows a remarkable similarity to those calculated for a proposed intermediate in the enantioselective dihydroxylation of olefins by osmium tetraoxide.