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GNEN-1 : a spontaneously immortalized cell line from gastric neuroendocrine neoplasia
- Source :
- Endocrine Connections, Vol 10, Iss 9, Pp 1055-1064 (2021), Endocrine Connections
- Publication Year :
- 2021
-
Abstract
- Export Date: 15 September 2021 Correspondence Address: Fagerstedt, K.W.; Department of Pathology, Finland; email: klaus.wj.fagerstedt@helsinki.fi Mixed neuroendocrine-non-neuroendocrine neoplasms (MINEN) are rare tumors that consist of at least 30% of both neuroendocrine and non-neuroendocrine components. The data concerning the pathogenesis of MINEN suggest a monoclonal origin. We describe a spontaneously immortalized cell line derived from gastric MINEN called GNEN-1. Primary tumor consisted of components of high-grade neuroendocrine carcinoma and adenocarcinoma. The GNEN-1 cell line was initiated from metastatic tumor cells of peritoneal fluid and expresses a purely neuroendocrine phenotype. The GNEN-1 cell line grows as monolayers and has retained the neuroendocrine phenotype with positivity for chromogranin A in immunohistochemistry. Electron microscopy showed cytoplasmic dense core granules and axon hillocks. The karyotype revealed alterations typical of both adenocarcinoma and neuroendocrine carcinoma such as trisomy 7 and 8. GNEN-1 cells were also positive for stanniocalcin-1, a marker of poor prognosis in gastric carcinomas. Expression of several markers related to neuroendocrine tumors was found. There have been only a few studies on the pathogenesis of MINEN and management of the disease due to the rarity of this tumor type. Here we describe for the first time an immortalized cell line derived from mixed gastric NEN. The GNEN-1 line offers a tool for future research on gastric NEN. © 2021 The authors.
- Subjects :
- chromogranin A
synaptophysin
cisplatin
cholecystectomy
etoposide
computer assisted tomography
spontaneously immortalized cell line
REG-IV
genome analysis
clinical article
neuroendocrine carcinoma
hypocalcin
cell line
karyotyping
CANCER
TUMORS
CYTOGENETIC CHARACTERIZATION
Neuroendocrine
eosin
real time polymerase chain reaction
immunohistochemistry
glutaraldehyde
histopathology
MINEN
STC1
gastroscopy
EXPRESSION
phenotype
comparative genomic hybridization
cancer prognosis
Diseases of the endocrine glands. Clinical endocrinology
Article
histology
trisomy 8
transmission electron microscopy
trisomy 7
case report
MIXED ADENONEUROENDOCRINE CARCINOMA
controlled study
human
axon hillock
adenocarcinoma
osmium tetraoxide
Research
hematoxylin
human cell
gastric cancer
RC648-665
ISL1
human tissue
RNA extraction
karyotype
liver metastasis
gene expression
3111 Biomedicine
cytokeratin
paraformaldehyde
stomach carcinoma
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Endocrine Connections, Vol 10, Iss 9, Pp 1055-1064 (2021), Endocrine Connections
- Accession number :
- edsair.pmid.dedup....6e7842c374120ccda105e3a237f7ac64