Search

Stable and efficient partial nitrification reaction and nitrite accumulation are essential for the operation of partial nitrification-anaerobic ammonia oxidation process. This paper investigated the changes in the performance of a partial nitrification bioreactor during the domestication process. After 40 days of domestication, the partial nitrification reaction remained stable, the nitrite accumulation rate (NAR) increased significantly to 85.7%, and the NO2--N/NH4+-N ratio stabilized at 1.25 ± 0.04. Both diversity indices (Simpson and Shannon) and richness indices (Ace and Chao1) tend to decrease with domestication. Nitrosomonas as a typical ammonia oxidizing bacterium it increased its relative abundance in the reactor from 0.02 ± 0.007% at P1 to 11.6 ± 2.2% at P2, and at P3 stage the denitrifying functional bacteria norank_f_AKYH76, Dokdonella and unclassified_f_Comamonadaceae reached 33.4 ± 12.5%, 18.8 ± 1.8%, and 10.1 ± 1.3%, respectively, and the increase in the relative abundance of these functional species was also consistent with the accumulation of nitrite nitrogen in the reactor. [ABSTRACT FROM AUTHOR]

Composting is a sustainable and eco-friendly technology that turns animal waste into organic fertilizers. It remains unclear whether differences exist in the structure of microbial communities during different livestock manure composting. This study analyzed the dynamic change of bacterial and fungal communities, metabolic function, and trophic mode during chicken manure (CM) and pig manure (PM) composting based on 16S rRNA and ITS sequencing. Environmental factors were investigated for their impact on microbial communities. During composting, bacterial diversity decreased and then increased, while fungal diversity slightly increased and then decreased. Saccharomonospora and Aspergillus were the dominant genera and key microorganisms in CM and PM, respectively, which played crucial roles in sustaining the stability of the ecological network structure in the microbial ecology and participating in metabolism. Saccharomonospora gradually increased, while Aspergillus increased at first and then decreased. PM had better microbial community stability and more keystone taxa than CM. In CM and PM, the primary function of bacterial communities was metabolism, while saprotroph was the primary trophic mode of fungal communities. Dissolved organic carbon (DOC) was the primary factor influencing the structure and function of microbial communities in CM and PM. In addition to DOC, pH and moisture were important factors affecting the fungal communities in CM and PM, respectively. These results show that the succession of bacteria and fungi in CM and PM proceeded in a similar pattern, but there are still some differences in the dominant genus and their responses to environmental factors. [ABSTRACT FROM AUTHOR]

Simple Summary: Pelteobagrus fulvidraco is a common freshwater fish, mainly distributed in China, Vietnam, Laos, and southeast Siberia of Russia. Its meat is delicious and has high nutritional value. The purpose of the study was to explore the suitable farming density of P. fulvidraco in integrated rice–fish farming. Our study revealed that the stocking density of P. fulvidraco in a paddy field should not exceed 250 g/m2. High stocking density inhibited growth performance and caused physiological response. These insights offer guidance for the selection of an appropriate stocking density for P. fulvidraco in integrated rice–fish farming systems. Pelteobagrus fulvidraco is a freshwater fish commonly raised in rice fields, yet the optimal stocking density for this species remains unknown. Therefore, this study aimed to investigate the appropriate stocking density of P. fulvidraco in integrated rice–fish farming systems. Three different stocking densities––low density (LD, 125 g/m2), middle density (MD, 187.5 g/m2), and high density (HD, 250 g/m2)––were set up to evaluate P. fulvidraco's growth performance, stress indices, immune function, antioxidant status, and lipid metabolism after 90 days of farming. The results indicated that HD treatment had a detrimental effect on P. fulvidraco's growth parameters. HD treatment led to an increase in cortisol (Cor) and lactate (La) levels, but a decrease in glucose (Glu) content in serum. After 90 days of farming, an immune response accompanied by the increase of complement 3 (C3), C4, and immunoglobulin M (IgM) was observed in the HD group. Meanwhile, HD treatment induced oxidative stress and altered antioxidative status evidenced by the levels of catalase (CAT), glutathione peroxidase (Gpx), glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) in serum or liver. Additionally, the lipid metabolism-related genes including lipoprotein lipase (lpl), peroxisome proliferators-activated receptor (pparα), carnitine palmitoyltransferase-1 (cpt-1), and sterol regulatory element binding protein-1 (srebp-1) were markedly downregulated in the HD and/or MD group after 90 days of farming. In conclusion, this study contributes to a better understanding of P. fulvidraco's response to different stocking densities in integrated rice–fish farming systems. We suggest that the appropriate stocking density for P. fulvidraco in these farming systems should be below 250 g/m2, considering both fish growth and physiological responses. [ABSTRACT FROM AUTHOR]

The beneficial effects of biochar addition during composting have been proved for many feedstocks, like manures and crop straws. However, the effect of biochar on the quality of composting product with seaweed as the feedstock and the bacterial response has not been investigated. In this study, the wheat straw biochar addition on the quality of the composting product and the bacterial response was explored at the rate of 0-10%. The results showed that biochar addition at the optimal rate (5%, w/w) could increase the germination index and the ratio of the optical density of humic acid at 460 nm to that at 660 nm (E4/E6) of the composting product, which indicated the decreased biotoxicity and enhanced compost maturity. The significant increase of the nitrate nitrogen (NO3--N) content of the composting product proved the improvement of N cycling during composting process with biochar addition. The bacterial community of composting product was shifted and the relative abundance of some beneficial taxa (e.g., Muricauda and Woeseia) was significantly increased with biochar addition. Furthermore, the relative abundance of some bacterial genes related to amino acid metabolism and carbohydrate metabolism was also increased with biochar addition. The results of our study provided the positive effect of biochar addition on the composting of seaweed and could help to produce high quality seaweed fertilizer by composting with biochar addition. [ABSTRACT FROM AUTHOR]

This study investigates the co-occurrence of per- and polyfluoroalkyl substances (PFASs), petroleum hydrocarbons (TPHs) and benzene, toluene, ethylbenzene, and xylene (BTEX) and their effects on the indigenous microbial communities in soils at a contaminated site with a history of petroleum refinery operations. PFASs concentrations were in the range of 5.65-6.73 ng/g, and fluorooctane sulfonate (PFOS) and perfluorobutane sulfonic acid (PFBS) were the dominating compounds. No significant difference was observed in the PFASs concentration profiles between the site and background locations, indicating that PFASs detected in the soil samples were mainly contributed from non-point sources, due to the long-distance transport of PFASs in the region. The concentrations of TPHs and BTEX ranged from 227 to 72,360 mg/kg and 0.06-2109.77 mg/kg, respectively, mainly contributed by the historical refinery activities. The presence of PFASs, TPHs, and BTEX significantly impacted soil microbial community diversity and abundance, altering microbial compositions and enriching bacteria with higher resistance or metabolic capabilities against contamination. Strong correlations were observed between TPHs and its degraders such as Pseudomonas, Azoarcus, and Polaromonas. Significant positive relationship between PFASs and Trichlorobacter implied the potential defluorination capabilities of Trichlorobacter, warranting further investigation. Moreover, the higher energy metabolism including carbon, nitrogen and sulfur metabolisms and higher abundance of metabolic enzymes for alkane, cyclohexane and toluene in the refinery site revealed the potential occurrence of natural biodegradation of contaminants with indigenous microbial community. These findings highlight the complexity of sites contaminated with a mixture of traditional and emerging contaminants, providing valuable insights into the potential for biodegradation of mixed contaminants and underscoring the need for integrated approaches in environmental remediation strategies. This study contributes to understanding the ecological impacts of co-occurring contaminants and emphasizes the importance of considering multiple contaminant types in environmental risk assessments and remediation efforts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Broussonetia papyrifera has a high lignocellulose content leading to poor palatability and low digestion rate of ruminants. Thus, dynamic profiles of fermentation lignocellulose characteristics, microbial community structure, potential function, and interspecific relationships of B. papyrifera mixing with wheat bran in different ratios: 100:0 (BP100), 90:10 (BP90), 80:20 (BP80), and 65:35 (BP65) were investigated on ensiling days 5, 15, 30, and 50. The results showed that adding bran increased the degradation rate of hemicellulose, neutral detergent fiber, and the activities of filter paper cellulase, endoglucanase, acid protease, and neutral protease, especially in the ratio of 65:35. Lactobacillus, Pediococcus, and Weissella genus bacteria were the dominant genera in silage fermentation, and Pediococcus and Weissella genus bacteria regulated the process of silage fermentation. Compared with monospecific B. papyrifera silage, adding bran significantly increased the abundance of Weissella sp., and improved bacterial fermentation potential in BP65 (p < 0.05). Distance-based redundancy analysis showed that lactic acid bacteria (LAB) were significantly positive correlated with most lignocellulose content and degrading enzymes activities, while Monascus sp. and Syncephalastrum sp. were opposite (p < 0.05). Co-occurrence network analysis indicated that there were significant differences in microbial networks among different mixing ratios of B. papyrifera silage prepared with bran. There was a more complex, highly diverse and less competitive co-occurrence network in BP65, which was helpful to silage fermentation. In conclusion, B. papyrifera ensiled with bran improved the microbial community structure and the interspecific relationship and reduced the content of lignocellulose. [ABSTRACT FROM AUTHOR]

Pelteobagrus fulvidraco is a freshwater fish commonly raised in rice fields, yet the optimal stocking density for this species remains unknown. Therefore, this study aimed to investigate the appropriate stocking density of P. fulvidraco in integrated rice–fish farming systems. Three different stocking densities––low density (LD, 125 g/m2), middle density (MD, 187.5 g/m2), and high density (HD, 250 g/m2)––were set up to evaluate P. fulvidraco’s growth performance, stress indices, immune function, antioxidant status, and lipid metabolism after 90 days of farming. The results indicated that HD treatment had a detrimental effect on P. fulvidraco’s growth parameters. HD treatment led to an increase in cortisol (Cor) and lactate (La) levels, but a decrease in glucose (Glu) content in serum. After 90 days of farming, an immune response accompanied by the increase of complement 3 (C3), C4, and immunoglobulin M (IgM) was observed in the HD group. Meanwhile, HD treatment induced oxidative stress and altered antioxidative status evidenced by the levels of catalase (CAT), glutathione peroxidase (Gpx), glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) in serum or liver. Additionally, the lipid metabolism-related genes including lipoprotein lipase (lpl), peroxisome proliferators-activated receptor (pparα), carnitine palmitoyltransferase-1 (cpt-1), and sterol regulatory element binding protein-1 (srebp-1) were markedly downregulated in the HD and/or MD group after 90 days of farming. In conclusion, this study contributes to a better understanding of P. fulvidraco’s response to different stocking densities in integrated rice–fish farming systems. We suggest that the appropriate stocking density for P. fulvidraco in these farming systems should be below 250 g/m2, considering both fish growth and physiological responses.

Distilled grain waste (DGW) can be converted to organic fertilizer via aerobic composting process without inoculating exogenous microorganisms. To illustrate the material conversion mechanism, this study investigated the dynamic changes of bacterial community structure and metabolic function involved in DGW composting. Results showed that a significant increase in microbial community alpha diversity was observed during DGW composting. Moreover, unique community structures occurred at each composting stage. The dominant phyla were Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota, Myxococcota, and Chloroflexi, whose abundance varied according to different composting stages. Keystone microbes can be selected as biomarkers for each stage, and Microbispora, Chryseolinea, Steroidobacter, Truepera, and Luteimonas indicating compost maturity. Co-occurrence network analysis revealed a significant relationship between keystone microbes and environmental factors. The carbohydrate and amino acid metabolism were confirmed as the primary metabolic pathways by metabolic function profiles. Furthermore, nitrogen metabolism pathway analysis indicated that denitrification and NH3 volatilization induced higher nitrogen loss during DGW composting. This study can provide new understanding of the microbiota for organic matter and nitrogen conversion in the composting process of DGW. [ABSTRACT FROM AUTHOR]

Broussonetia papyrifera has a high lignocellulose content leading to poor palatability and low digestion rate of ruminants. Thus, dynamic profiles of fermentation lignocellulose characteristics, microbial community structure, potential function, and interspecific relationships of B. papyrifera mixing with wheat bran in different ratios: 100:0 (BP100), 90:10 (BP90), 80:20 (BP80), and 65:35 (BP65) were investigated on ensiling days 5, 15, 30, and 50. The results showed that adding bran increased the degradation rate of hemicellulose, neutral detergent fiber, and the activities of filter paper cellulase, endoglucanase, acid protease, and neutral protease, especially in the ratio of 65:35. Lactobacillus, Pediococcus, and Weissella genus bacteria were the dominant genera in silage fermentation, and Pediococcus and Weissella genus bacteria regulated the process of silage fermentation. Compared with monospecific B. papyrifera silage, adding bran significantly increased the abundance of Weissella sp., and improved bacterial fermentation potential in BP65 (p < 0.05). Distance-based redundancy analysis showed that lactic acid bacteria (LAB) were significantly positive correlated with most lignocellulose content and degrading enzymes activities, while Monascus sp. and Syncephalastrum sp. were opposite (p < 0.05). Co-occurrence network analysis indicated that there were significant differences in microbial networks among different mixing ratios of B. papyrifera silage prepared with bran. There was a more complex, highly diverse and less competitive co-occurrence network in BP65, which was helpful to silage fermentation. In conclusion, B. papyrifera ensiled with bran improved the microbial community structure and the interspecific relationship and reduced the content of lignocellulose.

[Display omitted] • Community assembly processes and its governing factors during composting are analyzed. • Deterministic assembly processes largely govern community composition for composting. • Assembly processes influence metabolic functions via shifts in community composition. • OM and temperature govern assembly processes throughout the stages. Although many studies have shown that microbial communities play important roles in organic waste composting due to the involvement of specific microbial taxa with metabolic functions, the underlying ecological processes of community assembly and governing factors remain elusive. Thus, a chicken manure composting experiment as a model system of microbially mediated organic waste composting was conducted. Ecological null modeling and metabolic functional prediction combined with electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) were used to quantify assembly processes governing bacterial community composition and functions during composting. The results showed the predominant role of deterministic assembly processes in shifting community compositions both across and within composting stages. Stochastic assembly processes also concomitantly influenced microbial community compositions. Changes in the organic matter (OM) content and its chemical properties and temperature governed bacterial community assembly processes throughout the stages by selecting specific bacterial taxa such as Cardiobacteriales, Bacteroidales, and Lachnospiraceae on day 1, Firmicutes on days 6, 25 and 37, and Sphingobacteriales, Thermoactinomycetaceae, Actinobacteria, and Novibacillus on day 45. These taxa ultimately influenced community functions such as environmental information processing, carbohydrate and amino acid metabolism, cellular processes, and genetic information processes involved in composting. Taken together, this study indicates that deterministic assembly processes governed by OM content and quality as well as temperature influenced microbial community turnover and determined community functions during composting. These results are important for better understanding and predicting microbial-driven composting and for ultimately manipulating microorganisms for environmentally-friendly composting outcomes. [ABSTRACT FROM AUTHOR]

We investigated the intestinal microbiota in shrimp fed diets with five replacement levels (0, 25%, 50%, 75% and 100%) of fish meal by fish silage. An 8‐week feeding trail was conducted in 15 fibreglass tanks (400 L volume, three replications for each diet) with 50 shrimps (initial weight 0.26 ± 0.03 g) per tank. After 8‐week trial, shrimp fed diets with low replacement levels (0% and 25%) of fish meal by fish silage had significantly enhanced growth performance (p < 0.05). Based on the Illumina sequencing of 15 samples, a total of 5,778 OTUs were obtained and the number of OTUs in each sample ranged from 2,346 to 3,491. OTUs were identified into 23 phyla, and Proteobacteria was the most abundant. At family and genus levels, family Vibrionaceae and genus Photobacterium were more abundant in high replacement level groups (50%, 75% and 100%) than those in low replacement level groups (0% and 25%), which may be related to the worse growth performance of high replacement level groups. However, dietary different levels of fish silage did not make significant difference on intestinal bacterial richness, diversity and metabolism functions (p > 0.05), which indicated the limited impact of dietary fish silage on intestinal microbiota. [ABSTRACT FROM AUTHOR]

Highlights • Earthworm casts and zeolite improved the thermophilic temperature of compost. • Earthworm casts and zeolite influenced the succession of bacterial community. • Bacterial community and function were related to temperature and NH 4 + content. Abstract Succession and metabolism functions of bacterial communities were determined in maize straw composting with earthworm casts and zeolite addition by using high-throughput sequencing, Biolog and PICRUSt. Results showed that earthworm casts and zeolite addition increased the temperature, decreased NH 4 + contents and affected bacterial community structure. The relative abundances of Firmicutes and Betaproteobacteria increased with earthworm casts and zeolite addition in the late stage. The abundances of genes related to carbohydrate metabolism, amino acid metabolism, and energy metabolism were increased by these two additives in the early stage, but decreased in the late stage. Network analysis demonstrated that members of Bacillaceae were identified as the keystone taxa. Temperature showed negative relationship with Georgenia , while NH 4 + exhibited positive associations with Georgenia , Devosia , Ruania and Mycobacterium. These results indicated that earthworm casts and zeolite addition benefitted the keystone species and enhanced the metabolism capacity of bacterial community, thereby improved the quality of compost. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • SND via the nitrite pathway was achieved when the DO was 0.7 ± 0.1 mg/L. • A novel short nitrification strategy was achieved by alternating the DO content. • The bacterial communities and metabolic functions of sludge were evaluated. • The relative content of denitrifying bacteria increased under low DO conditions. • Enzymes involved in nitrification and denitrification increased when DO decreased. Abstract In this study, the performance of simultaneous nitrification and denitrification via nitrite was investigated by alternating the dissolved oxygen (DO) concentration in a sequencing batch reactor with the DO-control area and the non-control area. In addition, bacterial communities and their metabolic functions were analyzed by high-throughput sequencing technology and phylogenetic investigation of the communities by reconstruction of unobserved states (PICRUSt). The removal efficiencies of NH 4 +-N and total nitrogen via the nitrite pathway were 97.91 ± 2.04% and 72.28 ± 2.23%, respectively, by maintaining low DO levels (0.7 ± 0.1 mg/L) in the DO-control area. PICRUSt analysis showed that the metabolic potential of the bacterial community for amino acids, nucleotides, coenzymes and inorganic ions decreased, while the relative abundance of key enzymes involved in nitrification and denitrification, and the relative population of denitrifying bacteria increased when the DO decreased from 1.2 ± 0.2 mg/L to 0.7 ± 0.1 mg/L. [ABSTRACT FROM AUTHOR]

Highlights • Wheat and maize straw biochar addition improved the maturity and fertility of compost product. • Biochar addition influenced the structure and functions of microbial community during composting process. • Network analysis revealed the close associations between bacterial taxa and the properties of compost. Abstract The present study determined the dynamic changes of bacterial community structures and its metabolism functions in rice straw composting process with different types of biochar addition. Results showed that both wheat and maize straw biochar addition greatly increased the temperatures and germination index. Biochar addition influenced microbial community structure and metabolic characteristics of the compost. Firmicutes , Actinobacteria and Proteobacteria were the dominant phyla throughout the process, and both biochar addition significantly increased the relative abundance of Actinobacteria phylum, especially genus Saccharomonospora. The abundance of bacterial genes related to amino acid metabolism, carbohydrate metabolism and energy metabolism were also increased with biochar addition. Moreover, total nitrogen was strongly affected by biochar addition and had the greatest influence on the bacterial community structure. These results indicated that biochar addition improved the maturity and fertility of the compost product as well as significantly regulated the microbial community structure and functions during composting process. [ABSTRACT FROM AUTHOR]

Abstract Biodrying was first used for post-treatment of storage sludge mixed with beer lees. In this study, dynamic changes in dissolved organic matter (DOM), bacterial community structure, bacterial associations as well as metabolic functions were investigated using Excitation-Emission Matrix (EEM) spectra, high-throughput sequencing, network and correlation matrix analyses, and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Furthermore, a hypothetical model was proposed to better understand the biodrying process. The results showed that desired performance was obtained and DOM variations revealed that biodrying can increase biostability of the matrix. The bacterial communities differed among different stages of the biodrying. At the phylum level, the dominant phyla were Proteobacteria and Bacteroidetes in the mesophilic and cooling phases, whereas Firmicutes became the most dominant phylum in the thermophilic phase. At the genus level, the dominant bacteria in the mesophilic and cooling phases were not obvious, while Ureibacillus and Bacillus were the dominant genera in the thermophilic phase. Network and correlation matrix analyses were useful tools for insights into the bacterial interactions. PICRUSt metagenome inference indicated that metabolism, genetic information processing, and environmental information processing were the primary metabolic pathways. These results allowed us to advance a hypothetical model explaining how succession in bacterial associations regulates the dynamics of a biodrying system. Graphical abstract Unlabelled Image Highlights • Biodrying was first used for treatment of storage sludge mixed with beer lees. • Bacterial associations were revealed by network and correlation matrix analyses. • Bacterial metabolic function was predicted by PICRUSt. • A hypothetical model to explain how changes in bacterial associations was proposed. [ABSTRACT FROM AUTHOR]

Succession of bacterial community, metabolism function and substrate utilization capacity in 60 days composting of cow manure were analyzed by 16S rRNA pyrosequencing, PICRUSt and Biolog method, respectively. The results showed that the number of bacterial OTUs increased from 176 in raw cow manure to 203 on Day-10, 220 on Day-30 and 313 on Day-60 of the composting, respectively. The PICRUSt analysis showed that the relative abundances of genes involved in lipid and carbohydrate metabolism increased by 28.5% and 22.4% during the incubation, respectively, but the abundances of the genes involved in nucleotide and amino acid metabolism decreased by 21.6% and 2.1%. Furthermore, the average well color development (AWCD) of carboxylic acids (0.99–0.48) and amino acids (1.61–0.89) in Biolog Eco-microplate displayed a steady downtrend through the composting process. Redundancy analysis showed that ORP, moisture and temperature could explain 68.1%, 17.6% and 14.2% of the variation in bacterial genera, respectively. [ABSTRACT FROM AUTHOR]

Highlights • Bacterial community succession was detected in swine manure composting with pumice. • Succession of metabolism function of bacterial community was analyzed by PICRUSt. • Organics utilization capacity of bacterial community was analyzed by Biolog EcoPlate. • Bacterial community and metabolism function succession was tightly related to ORP. Abstract Organics metabolic function of bacterial communities was evaluated in 60 days composting of swine manure and pumice by using MiSeq sequencing, PICRUSt and Biolog tools. The diversity of bacterial communities significantly decreased during the first 10 days, and gradually increased in the cooling and curing phase. The PICRUSt and Biolog analysis indicated that carbohydrate, lipid and amino acids metabolisms were relatively higher in the thermophilic phases. Xenobiotics biodegradation and metabolism, lipid metabolism, terpenoids and polyketides and biosynthesis of other secondary metabolites were mainly detected in the curing phases. Canonical correspondence analysis (CCA) indicated that the succession of bacterial community and organics utilization characteristics were highly affected by the temperature, moisture and oxidation reduction potential (ORP) in the swine composting system. [ABSTRACT FROM AUTHOR]

The metabolic function of microbial community dominated organics and nutrients transformation in aerobic composting process. In this study, the metabolic characteristics of bacterial and fungal communities were evaluated in 60 days composting of sludge and pumice by using FUNGuild and PICRUSt, respectively. The results showed that microbial community structure and metabolic characteristics were distinctively different at four composting periods. Bacterial genes related to carbohydrate metabolisms decreased during the first 30 days, but bacterial sequences associated with oxidative phosphorylation and fatty acids synthesis were enhanced in curing phase. Most of fungal animal pathogen and plant pathogen disappeared after treatment, and the abundance of saprotroph fungi increased from 44.3% to 97.8%. Oxidation reduction potential (ORP) significantly increased from −28 to 175 mV through incubation. RDA analysis showed that ORP was a crucial factor on the succession of both bacterial and fungal communities in sludge composting system. [ABSTRACT FROM AUTHOR]

[Display omitted] • Aerobic co-composting was performed by adding mature compost to cattle manure. • Addition of mature compost contributes to lignocellulosic degradation. • Mature compost increased carbohydrate-active enzymes activity. • Addition of mature compost increased the synergistic effect between microorganisms. The production of organic fertilizer by aerobic composting of cattle manure is an important way of its resource utilization. This study evaluated the effects of adding mature compost on the decomposition and microbial communities in the aerobic composting of cattle manure. The addition of mature compost shortens the composting cycle and results in a final lignocellulosic degradation rate of 35%. Metagenomic analysis showed that this was due to the proliferation of thermophilic and organic matter-degrading functional microorganisms, which enhanced the activity of carbohydrate-active enzymes. With the addition of mature compost, the microbial community exhibited stronger metabolic functions, especially carbohydrate and amino acid metabolism, which are the driving forces of organic matter degradation. This study deepens the understanding of organic matter conversion and microbial community metabolic functions when mature compost is used for livestock manure composting and provides a promising technology for livestock manure composting. [ABSTRACT FROM AUTHOR]

• Microorganisms of the coal flotation system come from the coal seam. • The isolated Bacillus sp. can grow in the coal slurry containing organic reagents. • Environmental factors stimulate succession by changing microbial functions. The water system in a coal preparation plant harbors a variety of microorganisms, which may pose a great effect on coal recovery, however, usually be neglected. In this study, high-throughput sequencing and the growth of indigenous microorganisms in coal slurry containing organic reagents were conducted to trace the sources and succession of the microorganisms in a running plant, and the reason for microbial community succession was deeply explained. The microbiological analysis results found that the microorganisms in the coal slurry are assigned to 34 phyla and 98 genera, all source from the coal seam. Proteobacteria, Acidobacteria, Actinobacteria, and Crenachaeota are the dominant microorganisms at the phylum level, and the abundance of Proteobacteria, Bacteroidetes, and Nitrospirae in the flotation feeding decrease, and the abundance of GAL15 increase compared to the raw coal and mine water samples. Environmental factors are important reasons for microbial community succession. Organic reagents such as collectors and frothers commonly used in coal flotation processes can change the growth environment of microorganisms. The strain QX4, isolated from raw coal and identified as Bacillus sp. can accelerate growth in coal slurry containing organic reagents. Compared with raw coal, the content of chemical oxygen demand (COD) and dissolved oxygen (DO) in the flotation feeding increase by 340 mg/l and 5 mg/l respectively, which inhibit the metabolism of Nitrospirae, Bacteroides, Proteobacteria, and Actinobacteria, while improving the metabolism of GAL15, and change the microbial community and promote the microbial succession in the coal flotation system. Therefore, indigenous microorganisms from coal seam can grow and succeed in the coal flotation system, which has a potential effect on coal flotation efficiency. [ABSTRACT FROM AUTHOR]

The beneficial effects of biochar addition during composting have been proved for many feedstocks, like manures and crop straws. However, the effect of biochar on the quality of composting product with seaweed as the feedstock and the bacterial response has not been investigated. In this study, the wheat straw biochar addition on the quality of the composting product and the bacterial response was explored at the rate of 0-10%. The results showed that biochar addition at the optimal rate (5%, w/w) could increase the germination index and the ratio of the optical density of humic acid at 460 nm to that at 660 nm (E4/E6) of the composting product, which indicated the decreased biotoxicity and enhanced compost maturity. The significant increase of the nitrate nitrogen (NO 3 - -N) content of the composting product proved the improvement of N cycling during composting process with biochar addition. The bacterial community of composting product was shifted and the relative abundance of some beneficial taxa (e.g., Muricauda and Woeseia ) was significantly increased with biochar addition. Furthermore, the relative abundance of some bacterial genes related to amino acid metabolism and carbohydrate metabolism was also increased with biochar addition. The results of our study provided the positive effect of biochar addition on the composting of seaweed and could help to produce high quality seaweed fertilizer by composting with biochar addition., Competing Interests: HJ, CH, JZ, XZ, and ZZ are employed by China Tobacco Guangxi Industrial Co., Ltd. This study received funding from China Tobacco Guangxi Industrial Co., Ltd. The funder had the following involvement in the study: study design, data collection, and the decision to submit for publication. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Jia, Chu, You, Li, Huang, Zhang, Zeng, Yao and Zhou.)

Composting is an efficient way of disposing agricultural solid wastes as well as passivating heavy metals (HMs). Herein, equivalent (3%) biochar (BC) or lime (LM) were applied in rice straw and swine manure composting, with no additives applied as control group (CK). The results indicated that both the additives increased NO 3 - -N content, organic matter degradation, humus formation, and HM immobilization in composting, and the overall improvement of lime was more significant. In addition, the additives optimized the bacterial community of compost, especially for thermophilic and mature phase. Lime stimulated the growth of Bacillus, Peptostreptococcus, Clostridium, Turicibacter, Clostridiaceae and Pseudomonas, which functioned well in HM passivation via biosorption, bioleaching, or promoting HM-humus formation by secreting hydrolases. Lime (3%) as additive is recommended in swine manure composting to promote composting maturity and reduce HM risk. The study present theoretical guidance in improving composting products quality for civil and industrial composting., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

[Display omitted] • CMs inoculation elevated lignocellulose degradation. • CMs inoculation promoted humification of composting. • Abundances of cellulolytic and ligninolytic microbes were enhanced by CMs addition. • CMs inoculation raised xenobiotics degradation and secondary metabolite synthesis. This study investigated effects of composite microbes (CMs) (Phanerochaete chrysosporium and Trichoderma longibrachiatum) on humification during co-composting of biogas residue, spent mushroom substrate and rice straw. Results showed that CMs inoculation elevated degradation ratios of cellulose, hemicellulose and lignin by 7.86%, 8.87% and 6.45%, and contents of humus and humic acid were correspondingly promoted by 15.5% and 23.6%, respectively. Relative abundances of bacteria associated with refractory macromolecules degradation (Flavobacterium , Anseongella and Actinomadura) and cellulolytic fungi (Hypocreales_Incertae_sedis, Hypocreaceae and Psathyrellaceae) were raised by CMs addition. Redundancy analysis demonstrated a positive correlation between microbial communities and temperature, fulvic acid and lignocellulose contents. Moreover, CMs inoculation promoted pathways of xenobiotics biodegradation and metabolism, and biosynthesis of other secondary metabolites, which was closely associated with lignocellulose degradation and humus formation. These results suggested that biological inoculation could enhance composting efficiency and improve compost quality, benefiting biogas residues composting. [ABSTRACT FROM AUTHOR]

Food waste (FW) is important object of resource utilization and source of antibiotic resistance genes (ARGs). This study investigated the effects of biodrying combined with inoculating mature compost (B&M) on the composting efficiency, succession of bacterial communities and their links with metabolism functions as well as the fate of ARGs during FW composting. The results showed that B&M could rapidly raise and maintain high relative abundance of Bacillaceae (66.59–94.44%) as well as composting temperature (45.86–65.86 ℃), so as to achieve the final maturity of FW composting in a short time by regulating microbial carbohydrate (14.02–15.31%) and amino acid metabolism (10.33–12.47%). Network analysis demonstrated that high temperature could effectively inhibit the proliferation and spread of potential bacterial hosts of ARGs and integrons including Lactobacillaceae, Enterobacteriaceae, Leuconostocaceae and Corynebacteriaceae during the first two days of composting. As a result, B&M significantly reduced the absolute (72.09–99.47%) and relative abundances (0.31–2.44 logs) of nearly all ARGs especially ermB , tetM , bla CTX-M and bla OXA. Present study deepened the knowledge of ARGs variation, succession and metabolism functions of bacterial communities when B&M processes were used for FW composting, suggesting a promising technology for reducing the transmission risk of ARGs and reaching maturity of FW composting. [Display omitted] • Biodrying and mature compost inoculation are promising schemes for compost maturity. • Biodrying and mature compost inoculation rapidly reduced ARGs in heating-up stage. • Lactobacillaceae and Enterobacteriaceae were potential bacterial hosts of ARGs. • Main pathways including carbohydrate and amino acid metabolism should be emphasized. [ABSTRACT FROM AUTHOR]

Bacterial communities are highly sensitive to environmental changes, but their metabolic functions may be convergent under similar ecological conditions. In order to test this environmental attribute of the bacterial community and verify the feasibility in using the bacterial metabolic data to divide functional units at the river basin scale, the surface sediments in three continuous spatial units of the main stream (MS), left tributary (LT), and right tributary (RT) of the Duliujian River basin were selected as the research objects. Therefore, 16S rDNA high-throughput sequencing and the PICRUSt platform were used to analyze the bacterial diversity and metabolic function of sediments in different units. The results showed that there were no significant differences for the Shannon and Simpson indices between the different river channels ( P >0.05); however, the bacterial diversity indices of Chao1, ACE, Observed&#95;species, and PD&#95;whole&#95;tree of LT and RT were significantly higher than those of the MS ( P <0.05). Moreover, the dominant bacterial phyla were not significantly different among the different river channels. In the present study, a total of 41 metabolic pathways were predicted based on the KEGG database, among which 34 metabolic pathways such as membrane transport, amino acid metabolism, and carbohydrate metabolism were significantly different. The functional metabolic abundance was higher in MS than that in RT and LT ( P <0.05); however, there was no significant difference between those of RT and LT ( P >0.05). Except for exchangeable phosphorus and nitrate nitrogen, there were no significant differences in other physicochemical properties among the different river channels ( P >0.05). Furthermore, pH and iron/aluminum-phosphorus were found to significantly affect the bacterial structure, and SOM and TN were found to significantly affect the bacterial metabolic function. Overall, the sediments of MS and tributaries (LT and RT) of the river were clearly divided into level-1 and level-2 groups, indicating that the metabolic abundance of the bacterial community can be used as the key indicator in the division of functional units at the river basin scale. This study provided a theoretical basis and technical support for ecological functional division and management in coastal river basins.

Nitrate and sulfate often coexist in organic wastewater. In this study, an internal circulation anaerobic reactor was conducted to investigate the impact of nitrate on sulfate reduction. The results showed that sulfate reduction rate dropped from 78.4% to 41.4% at NO 3 - /SO 4 2- ratios ranging from 0 to 1.03, largely attributed to the inactivity of acetate-utilizing sulfate-reducing bacteria (SRB) and preferential usage of nitrate of propionate-utilizing SRB. Meanwhile, high nitrate removal efficiency was maintained and COD removal efficiency increased with nitrate addition. Enhancement of propionate and butyrate degradation based on Modified Gompertz model and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) analysis. Moreover, nitrate triggered the shift of microbial community and function. Twelve genera affiliated to Firmicutes, Bacteroidetes and Proteobacteria were identified as keystone genera via network analysis, which kept functional stability of the bacterial community responding to nitrate stress. Increased nitrate inhibited Desulfovibrio , but promoted the growth of Desulforhabdus. Both the predicted functional genes associated with assimilatory sulfate reduction pathway (cysC and cysNC) and dissimilatory sulfate reduction pathway (aprA , aprB , dsrA and dsrB) exhibited negative relationship with nitrate addition. [Display omitted] • Nitrate addition enhanced propanoate and butanoate metabolism pathway. • Mechanisms of substrate competition between DB and SRB were revealed. • LefSe analysis and co-occurrence analysis of microbiome were performed. • Sulfate reduction pathway were inhibited by nitrate from PICRUSt2. [ABSTRACT FROM AUTHOR]

This study investigated the removal of phosphorus in a modified constructed rapid infiltration system (CRIS). The mechanism of phosphorus removal in the CRIS was studied from the perspective of enzymes, the microbial communities, and their metabolic functions. When the chemical oxygen demand (COD) concentration in the influent was increased from 550 to 650 mg/L to about 1100 mg/L, the total phosphorus (TP) removal efficiency increased, with an effluent TP concentration of about 0.16 mg/L in the CRIS. The matrix in the column contained iron and aluminium elements, which reacted with phosphate in the wastewater, resulting in phosphorus precipitation and, consequently, phosphorus removal. The maximum phosphine production was 1.73 and 0.24 mg/m3 in the matrix and the column ventilator. The alkaline phosphatase activity which had a positive effect on the phosphorus removal was increased in the upper layer of the column. High-throughput sequencing analysis showed that the dominant bacterial phylum in the CRIS was Proteobacteria (49.33–51.8%). The abundance of Dechloromonas in the lower and upper layer of the column was 1.51 and 0.67%. The gene numbers of the phosphatidylinositol signaling system, the phosphonate and phosphinate metabolism, and the phosphotransferase system were 24,468, 10,661, and 20,909 in the upper layer and 27,408, 13,476, and 31,192 in the lower layer of the column. Image 1 • 90% of TP can be removed by the CRIS. • PH 3 plays a role in TP removal in the CRIS. • Phosphonate metabolism and Phosphotransferase system were observed in the KEGG database. [ABSTRACT FROM AUTHOR]

• Microbial metabolic function, trophic mode, degradation behavior were evaluated. • Bacterial carbohydrates degradation ability was enhanced in high temperature stage. • Fungal degradation rate on carboxylic and amino acids was highest in curing stage. • Saprotroph was the main trophic mode of fungal communities through composting. • Bacterial degradation rate was affected by redox potential, pH and moisture. The metabolic function and organic degradation behavior of bacterial and fungal communities were detected in 60 days composting of chicken manure and pumice by using Biolog tools, PICRUSt and FUNGuild. Fungal diversity increased from 57 OTUs in fresh chicken manure to 109 OTUs in high temperature stage, while bacterial diversity decreased from 86 OTUs to 44 OTUs after composting treatment. The carbohydrates degradation ability of bacterial community was enhanced in the high temperature stage. Fungal community had relatively higher degradation rates of carboxylic acids and amino acids in the maturation stage. Saprotroph was the main trophic mode of fungal community during the incubation process. The fungal animal pathogen decreased from 12.5% to 1.2% after composting treatment. Bacterial community composition and substrates degradation rate were mainly influenced by redox potential, pH and moisture, while temperature was the main environmental factor influencing on organic degradation of fungal community. [ABSTRACT FROM AUTHOR]

• Degradation of SMX was positively related with activities of enzymes and microbes. • The combined biochar-compost would weaken each other's remediation effects. • HCGs increased the bacterial diversity but reducedthe bacterial richness. • LCGs enhanced the bacterial richness but reduced the bacterial diversity. • HCGs raised functional gene RAs of amino metabolism but lowered those of carbohydrate. Biochar and compost, two common amendments, were rarely conducted to investigate their combined influence on enzymatic activities and microbial communities in organic-polluted wetlands. This article described the effects of biochar/compost on degradation efficiency of sulfamethoxazole (SMX) and ecosystem responses in polluted wetland soil during the whole remediation process. 1% biochar (SB1) increased degradation efficiency of SMX by 0.067% ascribed to the increase of dehydrogenase and urease. 5% biochar (SB5) decreased degradation efficiency by 0.206% due to the decrease of enzymes especially for dehydrogenase. 2% compost (SC2), 1% biochar & 2% compost (SBC3), both 10% compost (SC10) and 5% biochar & 10% compost (SBC15) enhanced degradation efficiency by 0.033%, 0.015% and 0.222%, respectively, due to the increase of enzymes and biomass. The degradation efficiency was positively related to biomass and enzymatic activities. High-throughput sequencing demonstrated that HCGs (SB5, SC10, SBC15) improved the bacterial diversities but reduced richness through introducing more exogenous predominance strains and annihilated several inferior strains, while LCGs (SB1, SC2, SBC3) exhibited lower diversities but higher richness through enhanced the RAs of autochthonal preponderant species and maintained some inferior species. Additionally, HCGs raised the RAs of amino and lipid metabolism gene but lowered those of carbohydrate compared with LCGs. [ABSTRACT FROM AUTHOR]

• Tween-80 greatly influenced the bacterial community structure. • Tween-80 enhanced cellulase activity and bacteria that degrade cellulose and lignin. • Tween-80 enhanced the capacities for carbohydrate and amino acid metabolism. • Order Bacillales closely related to the metabolism of carbon sources. Metabolism by microorganisms is the basis of composting. In this study, the dynamic changes in the enzyme activity levels, bacterial community structure, and metabolism functions were investigated during chicken manure composting with an added bio-surfactant (rhamnolipid) or chemical surfactant (Tween-80). The results showed that rhamnolipid and Tween-80 improved the quality of the finished compost in terms of the C/N ratio, water-soluble carbon content, germination index, E4/E6 ratio, and the cellulase activity, especially with Tween-80. Furthermore, the bacterial communities were determined by high-throughput sequencing, and their metabolism functions were evaluated using the PICRUSt and Biolog methods. Tween-80 greatly influenced the bacterial community structure, where it enhanced the abundances of bacteria that degrade cellulose and lignin (e.g., members of the order Bacillales) and the capacities for carbohydrate and amino acid metabolism. Network analysis also showed that the order Bacillales was closely related to the metabolism of characteristic carbon sources, especially carbohydrates. [ABSTRACT FROM AUTHOR]