1. Enhanced production of Varv-A-like and cycloviolacin O23-like peptides in Aspergillus niger elicited-Oldenlandia umbellata cell culture.
- Author
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Saranya, S., Chellapandi, P., and Velayutham, P.
- Abstract
Cyclotides are small disulfide-rich peptides (28–37 amino acids) with a wide range of biological activities. The Rubiaceae family is a good source for cyclotide production, but the quantity produced is not sufficient. Therefore, we aimed to enhance cyclotide production in Oldenlandia umbellata (Rubiaceae family) cell cultures using a fungal elicitation process. Aspergillus niger was used as an elicitor to enhance the cyclotides in this plant. After elicitation, proteins were extracted and analyzed using 2-D gel electrophoresis, followed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. The results of this study showed a 3.4-fold increase in protein spot intensity in fungal elicitor-treated cultures compared to untreated cultures. Mass spectrometric data showed two intense peaks in the monoisotopic protonated mass. Peptide sequencing of these peaks yielded two sequence hits that matched known cyclotide sequences in the CyBase database. The present study identified Varv-A and cycloviolacin O23-like peptides with antimicrobial properties, low cell permeability and solubility, and a lack of hemolytic, allergenic, toxic, and aggregation properties. Varv-A-like and cycloviolacin O23-like peptides are predicted to exhibit anticancer and antiviral properties, respectively. In conclusion, the cyclotides identified in this study may improve drug design and protein engineering, highlighting their therapeutic potential in the pharmaceutical industry.Key message: The production of antimicrobial varv-A and cycloviolacin O23-like peptides in O. umbellata cell cultures was significantly increased by 3.4 times using A. niger elicitation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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