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Rapid detection of ceftriaxone-resistant Salmonella by matrix-assisted laser desorption–ionization time-of-flight mass spectrometry combined with the ratio of optical density.
- Source :
- Annals of Clinical Microbiology & Antimicrobials; 8/7/2024, Vol. 23 Issue 1, p1-9, 9p
- Publication Year :
- 2024
-
Abstract
- Background: The increased resistance rate of Salmonella to third-generation cephalosporins represented by ceftriaxone (CRO) may result in the failure of the empirical use of third-generation cephalosporins for the treatment of Salmonella infection in children. The present study was conducted to evaluate a novel method for the rapid detection of CRO-resistant Salmonella (CRS). Methods: We introduced the concept of the ratio of optical density (ROD) with and without CRO and combined it with matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) to establish a new protocol for the rapid detection of CRS. Results: The optimal incubation time and CRO concentration determined by the model strain test were 2 h and 8 µg/ml, respectively. We then conducted confirmatory tests on 120 clinical strains. According to the receiver operating characteristic curve analysis, the ROD cutoff value for distinguishing CRS and non-CRS strains was 0.818 [area under the curve: 1.000; 95% confidence interval: 0.970–1.000; sensitivity: 100.00%; specificity: 100%; P < 10<superscript>− 3</superscript>]. Conclusions: In conclusion, the protocol for the combined ROD and MALDI-TOF MS represents a rapid, accurate, and economical method for the detection of CRS. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14760711
- Volume :
- 23
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Annals of Clinical Microbiology & Antimicrobials
- Publication Type :
- Academic Journal
- Accession number :
- 178877970
- Full Text :
- https://doi.org/10.1186/s12941-024-00729-9