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In this study, the flavonoids in peony seed meal were separated and purified by microfiltration-nanofiltration secondary membrane separation method. The effects of material-liquid ratio, extraction temperature, ethanol volume fraction and extraction time on the extraction amount of total flavonoids were investigated by single-factor experiments, and the extraction process was optimized and validated by response surface methodology on the basis of single factors. Eight types of microfiltration membranes, including polyvinylidene fluoride (PVDF), polyethersulfone (PES), polytetrafluoroethylene (PTFE), polypropylene (PP), mixed cellulose (MCE), polyacrylonitrile (PAN), aqueous acetate (CA) and polyamide (PA), were selected for the primary separation of PSMF extracts. The nanofiltration membranes were homemade organosilicon/PA composite membranes in the laboratory. The results showed that the optimum extraction conditions for PSMF were 1:15 g/mL, 50 ℃, 70% ethanol volume fraction, 30 min, (240.28±2.25) μg/mL of PSMF extraction amount. The PA membrane had a better separation effect, and the composite membrane had a better purification effect on PSMF, and the water solubility of flavonoids was increased to more than 90% after separation. After calcination at 800 ℃, the residual mass of the raw material solution was 1.43% and that of the PA membrane separation was 0.76%, and the BTESE/PA composite membrane was reduced to 0.26%, with fewer impurities and higher purity. Comparing its antioxidant activity before and after separation, the ·OH, DPPH· scavenging rate and reducing power were improved after separation, and the O2−· scavenging rate decreased from 79.94% to 64.82%. In this study, the active components in peony seed meal were analyzed. PSMF would be a novel phytoflavonoid resource with rich composition and certain research space.

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The research goal was to qualify concentration of meloxicam (M), applied oral and by electrophoresis (EF) in meniscus and synovial liquid of the damaged knee ankle, because of sport injuries within the parachutists. Research sample included 8 sport male parachutists, age 28-48, divided into 2 groups with 4 examinees each. For the first group 0,015 g M, was per oral (1 tablet per day) applied during 5 days, and the second group of 4 parachutists for same drug dosage equally by electrophoresis (EF). Meniscus and synovial liquid samples were taken afterwards by menisectomy. By methods of liquid-mass spectrometry (HPCL) the drug concentration was quantified in micrograms of medicament per g of tissue, applied within the both ways. The research results were tested by Student t-test for small pairs of samples, and high significant concentration of drug was noted p<0,001, applied in one term EF in meniscus and synovial liquid in comparison to five day per oral drug application. By Eliminating, the local applied systems effects, and maximal concentration of drug, by longer maintaining in the targeted tissue, EF can be recommended as a method of choice in clinical praxis for sport injuries. Electrophoresis application of Meloxicam in targeting the knee joint provides significant saturation and better effectiveness of the drug compared to the per oral application [ABSTRACT FROM AUTHOR]