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Abstract In epithelia, apicobasal cell polarization is closely linked to cell-cell contact formation, both controlled by the conserved Crumbs (CRB) complex, which includes the transmembrane protein Crumbs (CRB3a) and adapter proteins PALS1, PATJ, and LIN7c. In MDCK II cells, a model for cell polarization, depletion of PALS1 - which binds to all CRB components - leads to defective cell polarization and improper distribution of tight junction proteins, resulting in severe epithelial barrier defects in 3D cyst models. This study investigated whether this phenotype is associated with transcriptional changes by analyzing wildtype (WT) and PALS1 knockout (KO) MDCK II cell lines grown under non-confluent conditions and in 3D cyst cultures. Our results indicate that the transition from non-confluent cells to 3D cysts involves numerous differentially expressed genes (DEGs) in both WT and KO cells. Importantly, the analyses revealed significant overlaps between WT and KO cells in their maturation processes, suggesting that most identified DEGs are linked to differentiation from non-confluent to polarized MDCK cells and likely not a result of PALS1 deficiency. Gene Ontology (GO) enrichment and over-representation analyses using REACTOME and KEGG databases confirmed these similarities. In contrast, the direct comparison of WT and KO cells at the two stages showed fewer DEGs and overlaps in associated biological processes and signaling pathways. DEGs associated with the 3D stage, in which the phenotype manifests, contain DEGs and pathways that were predominantly linked to cell cycle linked processes, centromere assembly, or DNA replication. Furthermore, the transcription of genes encoding key junction proteins, additional polarity proteins, and cell-substrate interaction proteins is less affected by the loss of PALS1, indicating that PALS1 influences the transcriptional profiles in epithelial cells as a modulating factor.

In epithelia, apicobasal cell polarization is closely linked to cell-cell contact formation, both controlled by the conserved Crumbs (CRB) complex, which includes the transmembrane protein Crumbs (CRB3a) and adapter proteins PALS1, PATJ, and LIN7c. In MDCK II cells, a model for cell polarization, depletion of PALS1 - which binds to all CRB components - leads to defective cell polarization and improper distribution of tight junction proteins, resulting in severe epithelial barrier defects in 3D cyst models. This study investigated whether this phenotype is associated with transcriptional changes by analyzing wildtype (WT) and PALS1 knockout (KO) MDCK II cell lines grown under non-confluent conditions and in 3D cyst cultures. Our results indicate that the transition from non-confluent cells to 3D cysts involves numerous differentially expressed genes (DEGs) in both WT and KO cells. Importantly, the analyses revealed significant overlaps between WT and KO cells in their maturation processes, suggesting that most identified DEGs are linked to differentiation from non-confluent to polarized MDCK cells and likely not a result of PALS1 deficiency. Gene Ontology (GO) enrichment and over-representation analyses using REACTOME and KEGG databases confirmed these similarities. In contrast, the direct comparison of WT and KO cells at the two stages showed fewer DEGs and overlaps in associated biological processes and signaling pathways. DEGs associated with the 3D stage, in which the phenotype manifests, contain DEGs and pathways that were predominantly linked to cell cycle linked processes, centromere assembly, or DNA replication. Furthermore, the transcription of genes encoding key junction proteins, additional polarity proteins, and cell-substrate interaction proteins is less affected by the loss of PALS1, indicating that PALS1 influences the transcriptional profiles in epithelial cells as a modulating factor. [ABSTRACT FROM AUTHOR]

Studies utilizing electron microscopy and live fluorescence microscopy have significantly enhanced our understanding of the molecular mechanisms that regulate junctional dynamics during homeostasis, development and disease. To fully grasp the enormous complexity of cell--cell adhesions, it is crucial to study the nanoscale architectures of tight junctions, adherens junctions and desmosomes. It is important to integrate these junctional architectures with the membrane morphology and cellular topography in which the junctions are embedded. In this Review, we explore new insights from studies using super-resolution and volume electron microscopy into the nanoscale organization of these junctional complexes as well as the roles of the junction-associated cytoskeleton, neighboring organelles and the plasma membrane. Furthermore, we provide an overview of junction- and cytoskeletal-related biosensors and optogenetic probes that have contributed to these advances and discuss how these microscopy tools enhance our understanding of junctional dynamics across cellular environments. [ABSTRACT FROM AUTHOR]

Cell adhesion proteins localize to epithelial and endothelial cell membranes to form junctional complexes between neighboring cells or between cells and the underlying basement membrane. The structural and functional integrities of these junctions are critical to establish cell polarity and maintain tissue barrier function, while also facilitating leukocyte migration and adhesion to sites of inflammation. In addition to their adhesive properties, however, junctional proteins can also serve important noncanonical functions in inflammatory signaling and transcriptional regulation. Intriguingly, recent work has unveiled novel roles for cell adhesion proteins as both signaling initiators and downstream targets during inflammation. In this review, we discuss both the traditional functions of junction proteins in cell adhesion and tissue barrier function as well as their noncanonical signaling roles that have been implicated in facilitating diverse inflammatory pathologies. [ABSTRACT FROM AUTHOR]

The gap between software design and implementation often results in a lack of clarity and precision. Formal languages, based on mathematical rules, logic, and symbols, are invaluable for specifying and verifying system designs. Various semi-formal and formal languages, such as JSON, XML, predicate logic, and regular expressions, along with formal models like Turing machines, serve specific domains. This paper introduces a new specification formal language, ObTFL (Object Transaction Formal Language), developed for general-purpose distributed systems, such as specifying the interactions between servers and IoT devices and their security protocols. The paper details the syntax and semantics of ObTFL and presents three real case studies—federated learning, blockchain for crypto and bitcoin networks, and the industrial PCB board with machine synchronization—to demonstrate its versatility and effectiveness in formally specifying the interactions and behaviors of distributed systems. [ABSTRACT FROM AUTHOR]

We successfully synthesized three-layered photocatalysts by modifying Bi nanoparticles on TiO2/BiVO4 bilayer composite films through a sol-gel process and sputtering. When exposed to ambient air, the surface of the prepared Bi nanoparticles oxidizes to form an amorphous ultra-thin Bi2O3 out layer. Under light exposure, this layer is reduced to metallic Bi, thanks to the band alignment between the Bi nanoparticles and TiO2/BiVO4 Z-scheme composite. The addition of Bi nanoparticles in the composite films improves visible-light absorption by the surface plasmon resonance (SPR), which contributes to the hot electron and enhances the photocatalytic characteristics. By constructing effective TiO2/BiVO4 Z-scheme heterostructures to facilitate photoinduced electron-hole pair separation and prevent recombination, Bi nanoparticles can efficiently capture photons and enhance the photocatalytic efficiency of semiconductors through the SPR effect. Optimizing the content of Bi nanoparticles decorated on the TiO2/BiVO4 Z-scheme composite film is a promising approach for designing a highly efficient photocatalyst, as evidenced by the performance of photoelectrochemical properties and RhB photodegradation ability.

Febrile status epilepticus (FSE) is an important risk factor for temporal lobe epilepsy and early identification of those at high risk for epilepsy is vital. In a rat model of FSE, we identified an acute (2 hrs) novel MRI signal where reduced T2 relaxation values in the basolateral amygdala (BLA) predicted epilepsy in adulthood; this T2 signal remains incompletely understood and we hypothesized that it may be influenced by vascular topology. Experimental FSE induced in rat pups reduced blood vessel density of the cortical vasculature in a lateralized manner at 2 hrs post FSE. Middle cerebral artery (MCA) exhibited abnormal topology in FSE pups but not in controls. In the BLA, significant vessel junction reductions and decreased vessel diameter were observed, together with a strong trend for reduced vessel length. Perfusion weighted MRI (PWI) was acutely increased cerebral blood flow (CBF) in cortex, amygdala and hippocampus of FSE pups that correlated to decreased T2 relaxation values compared to controls. This is consistent with increased levels of deoxyhemoglobin associated with increased metabolic demand. In summary, FSE acutely modifies vascular topological and CBF in cortex and BLA that may underlie acute MRI signal changes that predict progression to future epilepsy.

In logical terms, a 2D map is a polymorphic form of linear objects made from practical features like roads, cartos (geometrical representation of a collection of buildings or specific features), roundabouts, highways, and many more. As autonomous vehicle disrupts transportation and navigation, there is a need to detect and identify map features at a granular level with no human intervention. This paper focuses on one of the practical problems, i.e. determining whether a junction is part of roundabouts using the Map domain, which is a novel approach to the best of the knowledge of an author. In a roundabout, the junction is the node having entry or exit of roads in or away from it. Closed loops in roads are extracted by traversing each link and checking for the common junctions using a deterministic algorithm. Loops can be formed not only by roundabouts it can be present at any intersection of roads, from all the universal sets of junctions; identifying the pattern and finding the right junctions of a roundabout is a very challenging and exciting problem. The authors have solved this problem in two parts; data extraction is done by collecting all the roads of America and Europe and then features are extracted, which depend on logical and domain knowledge. Finally, for the fuzzy part of features, a machine model that provides an efficient accuracy of 81% is created as it reduces lots of manual effort for verifying and correcting junctions. This applied methodology is easy to implement. [ABSTRACT FROM AUTHOR]

With the understanding of microRNA (miRNA or miR) functions in tumor initiation, progression, and metastasis, efforts are underway to develop new miRNA-based therapies. Very recently, we demonstrated effectiveness of a novel humanized bioengineered miR-124-3p prodrug in controlling spontaneous lung metastasis in mouse models. This study was to investigate the molecular and cellular mechanisms by which miR-124-3p controls tumor metastasis. Proteomics study identified a set of proteins selectively and significantly downregulated by bioengineered miR-124-3p in A549 cells, which were assembled into multiple cellular components critical for metastatic potential. Among them, plectin (PLEC) was verified as a new direct target for miR-124-3p that links cytoskeleton components and junctions. In miR-124-3p-treated lung cancer and osteosarcoma cells, protein levels of vimentin, talin 1 (TLN1), integrin beta-1 (ITGB1), IQ motif containing GTPase activating protein 1 (IQGAP1), cadherin 2 or N-cadherin (CDH2), and junctional adhesion molecule A (F11R or JAMA or JAM1) decreased, causing remodeling of cytoskeletons and disruption of cell-cell junctions. Furthermore, miR-124-3p sharply suppressed the formation of focal adhesion plaques, leading to reduced cell adhesion capacity. Additionally, efficacy and safety of biologic miR-124-3p therapy was established in an aggressive experimental metastasis mouse model in vivo. These results connect miR-124-3p-PLEC signaling to other elements in the control of cytoskeleton, cell junctions, and adhesion essential for cancer cell invasion and extravasation towards metastasis, and support the promise of miR-124 therapy.

Pure water splitting by charged semiconductors and solar photons to concurrently generate H2 and O2 molecules has gained notable attention due to worldwide clean energy generation and storage. Despite significant advancements, challenges facing severe carrier recombination and sluggish electron transport continue to hinder the intrinsic efficiency of water splitting. Here, we fabricate two-dimensional graphitic carbon nitride-hybridized sulfur thionic polymorphs (CN/S100/S010 junctions) by incorporating photoactive semiconductors within lateral nanosheets. As a result, the charge rectification effect within the coplanar graphitic carbon nitride/S100/S010 junctions is induced by well-designed driving forces: favorable band offsets and cascade polarized surface work functions. During the water splitting process, the photogenerated electrons are sequentially transferred from graphitic carbon nitride to element semiconductor sulfur {100} and subsequently oriented to sulfur {010} facets. This unique behavior of charge migration within CN/S100/S010 photocatalysts contributes to impressive rates of H2 and O2 production, reaching 740 and 363 µmol g−1·h−1, respectively, nearly 13-fold higher than that of the parent carbon nitride. Comprehensive spectroscopic and theoretical analyses confirm the formation of CN/S100/S010 hierarchies with long-lived charge carriers during hydrogen energy production. This work introduces novel avenues for automatically orienting photogenerated carriers and holds promising prospects for clean energy production.

When modeling complex fluid networks using one‐dimensional (1D) approaches, boundary conditions can be imposed using zero‐dimensional (0D) models. An application case is the modeling of the entire human circulation using closed‐loop models. These models can be considered as a tool to investigate short‐term transient and stationary hemodynamic responses to postural changes. The first shortcoming of existing 1D modeling methods in simulating these sudden maneuvers is their inability to deal with rapid variations in flow conditions, as they are limited to the subsonic case. On the other hand, numerical modeling of 0D models representing microvascular beds, venous valves or heart chambers is also currently modeled assuming subsonic flow conditions in 1D connecting vessels, failing when transonic and supersonic flow conditions appear. Therefore, if numerical simulation of sudden maneuvers is a goal in closed‐loop models, it is necessary to reformulate the current methodologies used when coupling 0D and 1D models, allowing the correct handling of flow evolution for both subsonic and transonic conditions. This work focuses on the extension of the general methodology for the Junction Riemann Problem (JRP) when coupling 0D and 1D models. As an example of application, the short‐term transient response to head‐up tilt (HUT) from supine to upright position of a closed‐loop model is shown, demonstrating the potential, capability and necessity of the presented numerical models when dealing with sudden maneuvers. [ABSTRACT FROM AUTHOR]

Recently there have been reports that identify two transient receptor potential channels in cell–matrix junctions known as focal adhesions. These are the calcium channel TRP canonical 7 and the calcium-activated monovalent ion channel, TRP melastatin (TRPM) 4. Here, we report on the occurrence of TRPM4 in focal adhesions of fibroblasts. Of three commercial antibodies recognizing this channel, only one yielded focal adhesion staining, while the other two did not. The epitope recognized by the focal adhesion-localizing antibody was mapped to the extreme C-terminus of the TRPM4 protein. The other two antibodies bind to N-terminal regions of the TRPM4 proteins. Deletion of the TRPM4 gene by CRISPR/cas9 techniques confirmed that this channel is a bona fide focal adhesion component, while expression of full-length TRPM4 proteins suggested that processing may occur to yield a form that localizes to focal adhesions. Given the reports that this channel may influence migratory behavior of cells and is linked to cardiovascular disease, TRPM4 functions in adhesion should be explored in greater depth. (J Histochem Cytochem 71: 495–508, 2023) [ABSTRACT FROM AUTHOR]

E-beam voltage contrast inspection is a very common method for in-line detection of many key defect types for rapid yield learning during technology development. Generally, the wafer surface is charged positive, but sometimes charging the wafer surface negative makes more sense. This paper reviews four advantages that negative charging may provide. Switching from positive to negative charging is typically achieved using landing energy and/or extraction voltage. A third control knob is introduced and demonstrated using three common inspection layers, contact chemical mechanical polish (CMP), 3D NAND wordline shorts and 3D NAND wordline opens. [ABSTRACT FROM AUTHOR]

Accurate segmentation of power lines in various aerial images is very important for UAV flight safety. The complex background and very thin structures of power lines, however, make it an inherently difficult task in computer vision. This paper presents PLGAN, a simple yet effective method based on generative adversarial networks, to segment power lines from aerial images with different backgrounds. Instead of directly using the adversarial networks to generate the segmentation, we take their certain decoding features and embed them into another semantic segmentation network by considering more context, geometry, and appearance information of power lines. We further exploit the appropriate form of the generated images for high-quality feature embedding and define a new loss function in the Hough-transform parameter space to enhance the segmentation of very thin power lines. Extensive experiments and comprehensive analysis demonstrate that our proposed PLGAN outperforms the prior state-of-the-art methods for semantic segmentation and line detection. [ABSTRACT FROM AUTHOR]

Infrared (IR) small dim target detection near the sea–sky line (SSL) is crucial for enhancing the early warning capability of maritime vehicles. However, the interferences caused by the strong contrast have not been properly addressed. Consequently, a specially designed algorithm regional-division local contrast measure (RDLCM) that focuses on the detection of IR small dim targets appearing near the SSL is proposed. First, an SSL detection module based on a lightweight convolutional neural network (CNN) is devised to achieve fast pixel-level SSL detection. Then, a set of regional-division windows (RDWs) are designed according to the strong grayscale contrast distribution around the SSL, and through the division of the effective regions, the RDWs could realize the potential extraction and refinement of the IR small dim targets that appear near the SSL. Experiments on three IR image sequences demonstrate that the proposed algorithm achieves the best detection accuracy among the classical and state-of-the-art algorithms in comparison and runs at 44 frames per second (FPS), which could meet real-time requirements. The code and dataset are available at RDLCM. [ABSTRACT FROM AUTHOR]

The electrostatics of two-dimensional (2D) lateral pn homojunctions considering the impact of electrostatic doping by means of two split bottom-gates are studied here. Analytical expressions are obtained from the solution of the 2D Poisson equation considering a depletion approximation. Straightforward analytical models for the electrostatic potential and the depletion width within both the dielectric and the 2D semiconductor are obtained for both the symmetrical and asymmetrical cases. In contrast to the case of devices with chemical doping, the obtained depletion width model of devices with electrostatic doping do not depend on the dielectric constant but only on the electrostatic potential and oxide thickness. The models describe the electrostatics of gate-tunable 2D pn junctions at arbitrary bias. A benchmark against numerical device simulations of MoS $_{2}$ -based pn junctions validate the analytical models. [ABSTRACT FROM AUTHOR]

The lymphatic vasculature provides an essential route to drain fluid, macromolecules, and immune cells from the interstitium as lymph, returning it to the bloodstream where the thoracic duct meets the subclavian vein. To ensure functional lymphatic drainage, the lymphatic system contains a complex network of vessels which has differential regulation of unique cell-cell junctions. The lymphatic endothelial cells lining initial lymphatic vessels form permeable “button-like” junctions which allow substances to enter the vessel. Collecting lymphatic vessels form less permeable “zipper-like” junctions which retain lymph within the vessel and prevent leakage. Therefore, sections of the lymphatic bed are differentially permeable, regulated in part by its junctional morphology. In this review, we will discuss our current understanding of regulating lymphatic junctional morphology, highlighting how it relates to lymphatic permeability during development and disease. We will also discuss the effect of alterations in lymphatic permeability on efficient lymphatic flux in health and how it may affect cardiovascular diseases, with a focus on atherosclerosis. [ABSTRACT FROM AUTHOR]

Flexible electrodes using nanowires (NWs) suffer from challenges of long-term stability and high junction resistance which limit their fields of applications. Welding via thermal annealing is a common strategy to enhance the conductivity of percolated NW networks, however, it affects the structural and mechanical integrity of the NWs. In this study we show that the decoration of NWs with an ultrathin metal oxide is a potential alternative procedure which not only enhances the thermal and chemical stability but, moreover, provides a totally different mechanism to reduce the junction resistance upon heat treatment. Here, we analyze the effect of SnO x decoration on the conductance of silver NWs and NW junctions by using a four-probe measurement setup inside a scanning electron microscope. Dedicated transmission electron microscopy analysis in plan-view and cross-section geometry are carried out to characterize the nanowires and the microstructure of the junctions. Upon heat treatment the junction resistance of both plain silver NWs and SnO x -decorated NWs is reduced by around 80%. While plain silver NWs show characteristic junction welding during annealing, the SnO x -decoration reduces junction resistance by a solder-like process which does not affect the mechanical integrity of the NW junction and is therefore expected to be superior for applications. [ABSTRACT FROM AUTHOR]

During morphogenesis, large-scale changes of tissue primordia are coordinated across an embryo. In Drosophila, several tissue primordia and embryonic regions are bordered or encircled by supracellular actomyosin cables, junctional actomyosin enrichments networked between many neighbouring cells. We show that the single Drosophila Alp/Enigma-family protein Zasp52, which is most prominently found in Z-discs of muscles, is a component of many supracellular actomyosin structures during embryogenesis, including the ventral midline and the boundary of the salivary gland placode. We reveal that Zasp52 contains within its central coiled-coil region a type of actin-binding motif usually found in CapZbeta proteins, and this domain displays actin-binding activity. Using endogenouslytagged lines, we identify that Zasp52 interacts with junctional components, including APC2, Polychaetoid and Sidekick, and actomyosin regulators. Analysis of zasp52 mutant embryos reveals that the severity of the embryonic defects observed scales inversely with the amount of functional protein left. Large tissue deformations occur where actomyosin cables are found during embryogenesis, and in vivo and in silico analyses suggest a model whereby supracellular Zasp52-containing cables aid to insulate morphogenetic changes from one another. [ABSTRACT FROM AUTHOR]

The VPS13 protein family constitutes a novel class of bridge‐like lipid transferases. Autosomal recessive inheritance of mutations in VPS13 genes is associated with the development of neurodegenerative diseases in humans. Bioinformatic approaches previously recognized the domain architecture of these proteins. In this study, we model the first ever full‐length structures of the four human homologs VPS13A, VPS13B, VPS13C, and VPS13D in association with model membranes, to investigate their lipid transfer ability and potential structural association with membrane leaflets. We analyze the evolutionary conservation and physicochemical properties of these proteins, focusing on conserved C‐terminal amphipathic helices that disturb organelle surfaces and that, adjoined, resemble a traditional Venetian gondola. The gondola domains share significant structural homology with lipid droplet surface‐binding proteins. We introduce in silico protein‐membrane models displaying the mode of association of VPS13A, VPS13B, VPS13C, and VPS13D to donor and target membranes, and present potential models of action for protein‐mediated lipid transfer. [ABSTRACT FROM AUTHOR]

• • Complex 3D numerical modelling study of sprayed concrete lined tunnel junctions. • • Linear elastic models of the lining can lead to over conservative designs. • • Nonlinear elastic or strain hardening plastic models predicted lower lining loads. • • Using more sophisticated modelling of the lining could have saved time, cost and embodied carbon. This study presents a comprehensive analysis of numerical modelling techniques applied to the design and assessment of sprayed concrete-lined (SCL) tunnel junctions, focusing on the lining performance. The approach integrates advanced constitutive models to simulate both ground and lining responses, aiming to provide a robust understanding of tunnel behaviour under a realistically simulated construction sequence. Notable features of the modelling approach include nonlinear elastoplastic models for ground strata, depth-varying properties, variable K0 and pore pressure profiles, and step-by-step construction of cross-passages. The constitutive models for the ground have been validated against real site data, employing moderately conservative input parameters to ensure realistic comparisons with monitoring data. Results from numerical modelling offer insights into the lining movements, strains and loads at tunnel junctions. Notably, the choice of constitutive model for the lining significantly influences predicted behaviour, with nonlinear elastic models generally exhibiting larger but more localised movements and strains compared to linear elastic models. Furthermore, the study suggests potential optimisations in tunnel design, such as the reduction or elimination of reinforcement in thickening layers, based on the enhanced understanding provided by 3D numerical modelling. This underscores the value of this approach when considering environmental factors, such as carbon footprint reduction, in the design process. [ABSTRACT FROM AUTHOR]

The lymphatic vasculature provides an essential route to drain fluid, macromolecules, and immune cells from the interstitium as lymph, returning it to the bloodstream where the thoracic duct meets the subclavian vein. To ensure functional lymphatic drainage, the lymphatic system contains a complex network of vessels which has differential regulation of unique cell-cell junctions. The lymphatic endothelial cells lining initial lymphatic vessels form permeable “button-like” junctions which allow substances to enter the vessel. Collecting lymphatic vessels form less permeable “zipper-like” junctions which retain lymph within the vessel and prevent leakage. Therefore, sections of the lymphatic bed are differentially permeable, regulated in part by its junctional morphology. In this review, we will discuss our current understanding of regulating lymphatic junctional morphology, highlighting how it relates to lymphatic permeability during development and disease. We will also discuss the effect of alterations in lymphatic permeability on efficient lymphatic flux in health and how it may affect cardiovascular diseases, with a focus on atherosclerosis.

In this work, an acoustic emission (AE) technique was applied to online condition monitoring (CM) of solder fatigue in a clip-bonding SiC mosfet power assembly during short and long power cycling tests (PCTs). Solder fatigue caused by PCTs was identified in the clip-bonding assembly via scanning acoustic tomography and scanning electron microscopy and successfully diagnosed via the AE-based online CM. By analyzing AE signals, junction temperature, and junction-to-case thermal resistance, it can be confirmed that the AE signal shows a superior sensitivity in initial fatigue diagnosis to the thermal or electrical signals. Meanwhile, two solder fatigue processes, cracking initiation and cracking acceleration, were identified based on the cumulative AE counts and AE energy during the long PCT. An abrupt increase in AE energy happened at the end stage of the long PCT due to a severe cracking process, which can be regarded as a prewarning of a failure point of the power assembly. In addition, the relationship between transitional thermal resistance and accumulative AE energy was investigated and shows a linear relationship. These results suggest that the AE technique can be a promising online CM approach to diagnose and predict solder fatigue in power assemblies during operation. [ABSTRACT FROM AUTHOR]

Despite structural differences between the right-handed conformations of A-RNA and B-DNA, both nucleic acids adopt very similar, left-handed Z-conformations. In contrast to their structural similarities and sequence preferences, RNA and DNA exhibit differences in their ability to adopt the Z-conformation regarding their hydration shells, the chemical modifications that promote the Z-conformation, and the structure of junctions connecting them to right-handed segments. In this review, we highlight the structural and chemical properties of both Z-DNA and Z-RNA and delve into the potential factors that contribute to both their similarities and differences. While Z-DNA has been extensively studied, there is a gap of knowledge when it comes to Z-RNA. Where such information is lacking, we try and extend the principles of Z-DNA stability and formation to Z-RNA, considering the inherent differences of the nucleic acids. [ABSTRACT FROM AUTHOR]

Junction temperature is the most important factor to induce the insulated gate bipolar transistor module failure and conduct operational management. In-situ calibrated method and junction temperature calculation model are proposed to timely indicate the junction temperature for the electric vehicle drive system. It aims to solve that the conventional calibration methods need to be calibrated in the temperature chamber and the model cannot meet variable current conditions, which ignores the effects of the stray parameters of electrode connection on the on-state voltage. A mathematical junction temperature estimation model based on the relationship between saturation voltage drop and different currents is established, whose accuracy depends on the prior narrow range junction temperature calibration. A control strategy for the in-situ calibration is proposed to generate an instantaneous large current by creating a zero-torque condition and using integrated negative temperature coefficient thermistors, current sensor, and on-state voltage measured circuit when the new-energy vehicle is in an idle state. Experimental results prove that the proposed in-situ calibration method can be realized totally inside the electric vehicle, which can serve the full-service lifetime of the electric vehicle with high precision. [ABSTRACT FROM AUTHOR]

Owing to the superior performances, silicon carbide (SiC) metal oxide semiconductor field effect transistors (mosfets) attract a lot of attention. To increase the power density, it is desired to use the third quadrant (3rd-quad) characteristics of the mosfet rather than the externally paralleled Schottky diode for freewheeling during the deadtime. It has been known that the 3rd-quad is far more than a body diode, and the MOS channel is also an important part of it. The channel may be not fully closed and, therefore, play a significant role in the reverse conduction even when the gate is zero or negatively biased. However, a comprehensive study of the 3rd-quad characteristics is still to be conducted. In this article, experiments and simulations are conducted and a physical model is developed to explain the 3rd-quad characteristics of the SiC mosfet. It reveals how and why the 3rd-quad characteristics are affected by the gate voltage and the junction temperature. This article is helpful for not only the application of SiC mosfet but also the device design. [ABSTRACT FROM AUTHOR]

Thermal management is a cost-effective means to improve the lifetime of power module, but it also brings negative influence to the converter. To alleviate the negative effect, this article proposes a strategy to design the reference junction temperature swing based on the distribution characteristics of consumed lifetime and the thermal control efficiency. We show that the selected reference temperature swing, including its type and amplitude, allows each application of thermal control to reduce as much consumed lifetime as possible and, at the same time, to guarantee the performance of the converter. Moreover, this approach does not need to obtain the junction temperature swing in real time, which makes the thermal management simple and efficient. The experimental results are presented to verify the merits of the proposed strategy. This article also evaluates the impact of uncertainties due to the semiconductor parameter variations and performs the error analysis of junction temperature estimation. [ABSTRACT FROM AUTHOR]

Tracking the myotendinous junction (MTJ) motion in consecutive ultrasound images is essential to assess muscle and tendon interaction and understand the mechanics’ muscle-tendon unit and its pathological conditions during motion. However, the inherent speckle noises and ambiguous boundaries deter the reliable identification of MTJ, thus restricting their usage in human motion analysis. This study advances a fully automatic displacement measurement method for MTJ using prior shape knowledge on the Y-shape MTJ, precluding the influence of irregular and complicated hyperechoic structures in muscular ultrasound images. Our proposed method first adopts the junction candidate points using a combined measure of Hessian matrix and phase congruency, followed by a hierarchical clustering technique to refine the candidates approximating the position of the MTJ. Then, based on the prior knowledge of Y-shape MTJ, we finally identify the best matching junction points according to intensity distributions and directions of their branches using multiscale Gaussian templates and a Kalman filter. We evaluated our proposed method using the ultrasound scans of the gastrocnemius from 8 young, healthy volunteers. Our results present more consistent with the manual method in the MTJ tracking method than existing optical flow tracking methods, suggesting its potential in facilitating muscle and tendon function examinations with in vivo ultrasound imaging. [ABSTRACT FROM AUTHOR]

Intensive use of photovoltaic (PV) inverter in the volt/var control (VVC) methods in active distribution networks can impair inverter reliability. This article proposes a PV inverter reliability-constrained (PiReCon-) VVC method with a power smoothing scheme under uncertainties. First, considering impacts on inverter reliability, new reliability constraints are developed with a power smoothing factor to constrict inverter apparent power variation. Second, a new VVC optimization model is proposed with the reliability constraints, which minimizes power losses by optimizing both inverter var output and PV curtailment. Uncertainties of PV generation and loads are fully considered and addressed via a stochastic optimization method. Third, this article develops a penalty convex-concave programming method to effectively tackle nonconvexity of the proposed optimization model. The proposed PiReCon-VVC method is tested on a 33-bus distribution network, and simulation results verify its high efficiency in both minimizing the power losses and enhancing the PV inverter reliability. [ABSTRACT FROM AUTHOR]

Organic p–n junctions attract widespread interest in the field of molecular electronics because of their unique optoelectronic singularities. Importantly, the molecular donor/acceptor character is strongly correlated to the degree of substitution, e.g., the introduction of electron‐withdrawing groups. Herein, by gradually increasing the degree of peripheral fluorination on planar, D4h−symmetric iron(II) phthalocyanato (FePc) complexes, the energy level alignment and molecular order is defined in a metal‐supported bilayered Pc‐based junction using photoemission orbital tomography. This non‐destructive method selectively allows identifying molecular levels of the hetero‐architectures. It demonstrates that, while the symmetric fluorination of FePc does not disrupt the long‐range order and degree of metal‐to‐molecule charge transfer in the first molecular layer, it strongly impacts the energy alignment in both the interface and topmost layer in the bilayered structures. The p–n junction formed in the bilayer of perhydrogenated FePc and perfluorinated FeF16Pc may serve as an ideal model for understanding the basic charge‐transport phenomena at the metal‐supported organic–organic interfaces, with possible application in photovoltaic devices. [ABSTRACT FROM AUTHOR]

Temperature sensitive electrical parameters (TSEP) provide an indirect and noninvasive method for on-line junction temperature estimation of power semiconductor devices. It is known that the fundamental of TSEP-based methods is to calibrate the relationship between TSEP and junction temperature in advance. However, the calibration methods in the literature need to open the module, require pretesting, or record the operating data of a converter in the entire power rating range, which are inconvenient in field applications. This article proposes an on-line and noninvasive calibration method by measuring the data at three operating states that are already existed in the regular converter operation. It is achieved by measuring the accessible heatsink/case temperature and TSEP during converter regular operation. The concept, implementation, and error analysis of the proposed method are presented in this article. Experimental verification is given to prove the effectiveness, accuracy, and convenience of the proposed method. [ABSTRACT FROM AUTHOR]

This article investigates the effects of the average power variation and the ambient temperature on the behavioral modeling and predistortion of high-power amplifiers concurrently. The measurement results showed that in addition to the average power variation, the behavior of a high-power PA is a function of ambient temperature as well. This article proposes a novel smart digital predistortion (DPD) model that considers both average power variation and temperature (PTI-DPD). The PTI-DPD is implemented using artificial intelligence-based model and it benefits from sets of data formats containing the signal, its delayed versions, average power variation, and ambient temperature. The developed architecture provides an uninterrupted linearization across the ambient temperature and average power variation span without need for a continues feedback path or multiple models. A series of measurements have been conducted to study the PTI-DPD performance across the temperature and average power range. According to the results, the PTI-DPD achieves an average of −48.5 dBc adjacent channel power ratio across the average power and temperature span. [ABSTRACT FROM AUTHOR]

The current limiting function enables the solid-state circuit breaker (SSCB) to have proper protection coordination. It allows sustained overcurrent for a certain period while preventing the fast fault current increase in dc systems. For the conventional method of using switches alone to limit the current, the high loss results in a short withstand time and low current limiting capability of the SSCBs. In this letter, a control strategy is proposed to utilize the energy absorption component to handle the major part of the energy during the current limiting stage to increase the current limiting capability for series-connected SSCB switching cells. The proposed method is experimentally verified to have more than 3X current limiting withstand time compared to the conventional control strategy in a case study. [ABSTRACT FROM AUTHOR]

Junction temperature monitoring is the basis of high reliability operation for silicon carbide (SiC) devices, since thermal stress is the dominating aging factor. Conventional thermal sensitive electrical parameter methods have a poor monitoring performance for SiC Mosfets with low sensitivity. Thus, a high-sensitivity online junction temperature monitoring method is proposed based on the turn-on drain–source current overshoot. First, theoretical analysis is provided to clarify that the temperature difference between the body diode region and the on-state resistance region can be ignored in SiC Mosfets. It is an important basis for the proposed method, which is proven by an isothermal experiment. It is the first time to discover that the turn-on current overshoot (ΔiCm) of SiC Mosfets consists of both the reverse recovery current (iRR) and the intrinsic current overshoot (Δi0m). Next, a novel Tj monitoring strategy for SiC Mosfets is proposed based on comprehensive utilization of iRRm, Δi0m and ΔiCm. Finally, the effectiveness of the proposed method is verified by online experiments based on a dc–dc converter. The proposed method is more accurate than conventional TSEP methods with a high sensitivity of 20 mA/°C. Moreover, it still has a high monitoring performance when boundary conditions change. [ABSTRACT FROM AUTHOR]

Pulsed readout of Direct Current (DC) Superconducting Quantum Interference Device (SQUID) is crucial for experiments which need to be performed at millikelvin temperatures, such as the readout of superconducting and electron spin based qubits. Pulsed readout algorithms used in these experiments are usually specific to the experimental setup and require some optimization. We present a circuit that emulates the behavior of a DC-SQUID in order to allow the development and evaluation of pulsed readout algorithms at room temperature without the need of a running dilution refrigerator. This novel circuit also constitutes a low cost device which can be used to teach the principles of a DC-SQUID in courses aimed at training the next generation of quantum engineers. [ABSTRACT FROM AUTHOR]

With the understanding of microRNA (miRNA or miR) functions in tumor initiation, progression, and metastasis, efforts are underway to develop new miRNA-based therapies. Very recently, we demonstrated effectiveness of a novel humanized bioengineered miR-124-3p prodrug in controlling spontaneous lung metastasis in mouse models. This study was to investigate the molecular and cellular mechanisms by which miR-124-3p controls tumor metastasis. Proteomics study identified a set of proteins selectively and significantly downregulated by bioengineered miR-124-3p in A549 cells, which were assembled into multiple cellular components critical for metastatic potential. Among them, plectin (PLEC) was verified as a new direct target for miR-124-3p that links cytoskeleton components and junctions. In miR-124-3p-treated lung cancer and osteosarcoma cells, protein levels of vimentin, talin 1 (TLN1), integrin beta-1 (ITGB1), IQ motif containing GTPase activating protein 1 (IQGAP1), cadherin 2 or N-cadherin (CDH2), and junctional adhesion molecule A (F11R or JAMA or JAM1) decreased, causing remodeling of cytoskeletons and disruption of cell–cell junctions. Furthermore, miR-124-3p sharply suppressed the formation of focal adhesion plaques, leading to reduced cell adhesion capacity. Additionally, efficacy and safety of biologic miR-124-3p therapy was established in an aggressive experimental metastasis mouse model in vivo. These results connect miR-124-3p−PLEC signaling to other elements in the control of cytoskeleton, cell junctions, and adhesion essential for cancer cell invasion and extravasation towards metastasis, and support the promise of miR-124 therapy.

This article studies the influence of fiber Bragg grating (FBG) head length on insulated gate bipolar transistor (IGBT) direct on-chip thermal sensing performance of FBG sensors. To this end, the surface of a commercial IGBT chip is thermally simulated and experimentally characterized. Uniform FBG sensors with three head sizes are then tested in two promising thermal sensing locations. The study has found that the large thermal gradients in this application create an additional constraint when using longer head lengths. A distortion in the reflected spectrum of the 5-mm FBG sensor is used to illustrate the underlying physical effect, which causes this limitation for IGBT junction temperature measurement. This additionally affects the length of head sizes providing accurate temperature readings of the IGBT surface hotspots, and significantly, this limit is location-dependent in a given IGBT geometry. [ABSTRACT FROM AUTHOR]

Excellent high-temperature performance (298–523 K) of the 4H-SiC n-p-n bipolar phototransistor detector (PTD) was explored. It was fabricated using the 4H-SiC epitaxial wafer grown by high-temperature chemical vapor deposition (HTCVD). The static and dynamic properties of the device were tested and analyzed. The results show that the device still has an optical gain of up to 103, a wide linear response range, and good repeatability at the high temperatures up to 523 K under a 3-V bias with a wavelength of 360 nm. The temperature coefficient of the optical gain is extracted, the value of which is 1.02/K. Although the response time of the device increases with the temperature, the increased percentage in responsivity is significantly greater than that of the response time. The results provide an important experimental reference for the application of high-temperature detection and suggest that the PTD has the potential to be used in ultraviolet (UV) detecting systems at high temperatures. [ABSTRACT FROM AUTHOR]

The goal of molecular optimization is to generate molecules similar to a target molecule but with better chemical properties. Deep generative models have shown great success in molecule optimization. However, due to the iterative local generation process of deep generative models, the resulting molecules can significantly deviate from the input in molecular similarity and size, leading to poor chemical properties. The key issue here is that the existing deep generative models restrict their attention on substructure-level generation without considering the entire molecule as a whole. To address this challenge, we propose Molecule-Level Reward functions (MOLER) to encourage (1) the input and the generated molecule to be similar, and to ensure (2) the generated molecule has a similar size to the input. The proposed method can be combined with various deep generative models. Policy gradient technique is introduced to optimize reward-based objectives with small computational overhead. Empirical studies show that MOLER achieves up to 20.2% relative improvement in success rate over the best baseline method on several properties, including QED, DRD2 and LogP. [ABSTRACT FROM AUTHOR]

LED lighting still suffers from its thermal problem linked mainly to the increase in junction temperature. The situation becomes more and more aggravated when it comes to decorative lighting. Indeed, this type of lighting application sometimes requires that the heat sink of the LED lamp be integrated into a narrow space, such as false or sloped ceilings, which limits the transfer of heat to the outside. In this article, the current study reports a numerical analysis of natural convection heat transfer around rectangular heat sink for LEDs lighting applications. The radiator is put in an inclined cavity with an orientation angle varying from 0° to 60°. The effects of the enclosure inclination and its length on thermal behavior, flow structure in the cavity as well as on optical properties of the light source were highlighted. The CFD simulation showed a significant effect on the heat transfer rate when varying the cavity length (presented as an aspect ratio A) and its orientation. A = 4 was determined as an optimal cavity aspect ratio, and it was found that higher orientations are more sensitive to the increase of the length of the cavity. For θ = 60°, the luminous efficiency is ameliorated by about 10% when increasing the cavity length from one to four. Accordingly, we proposed correlations in simple form to predict the heat transfer rate for different configurations studied. [ABSTRACT FROM AUTHOR]

The impact of ring-shaped collector contact (RCC) on total ionizing dose (TID) susceptibility of 40-V vertical n-p-n bipolar transistors is investigated. The 60Co $\gamma $ -ray irradiation experiment shows that the devices with RCC suffer less current gain degradation than their counterparts with conventional collector contact structure, which is independent of the emitter area and bias condition during irradiation. Technology computer-aided design (TCAD) simulation results indicate that the enhancement in radiation tolerance of the RCC devices should be attributed to the mitigation of trap-assisted carrier recombination at the Si/SiO2 interfaces near emitter–base (EB) junction rather than the concentration or distribution variations of radiation-induced traps. Moreover, the mitigation of carrier recombination in RCC devices could result from the creation of new current flow paths that suppress the diffusion of injected electrons toward the trap-enriched Si/SiO2 interfaces. [ABSTRACT FROM AUTHOR]

In this article, the influence of thermal coupling in power modules on lifetime under power cycling test (PCT) is investigated. In a full-bridge power module for electric vehicle application, the thermal coupling is strong due to high power density and causes lateral temperature difference. In PCT, at the same maximum junction temperature Tvjmax and temperature swing ΔTvj, the influence of thermal coupling on lifetime and failure mode is investigated. The root cause of why thermal coupling influences lifetime and the failure mode is discussed by finite-element analysis. Transient thermal impedance is used to calibrate the simulation model. The Influence of thermal coupling on chip surface and solder layer temperature distribution is analyzed under the same test conditions. Furthermore, two parameters are extracted from the temperature distribution of chip surface as flags of failure mode determination. Finally, taking thermal coupling into account, combined with direct copper bonding structure, an improved layout is recommended in terms of lifetime. [ABSTRACT FROM AUTHOR]

In this article, an electrothermal model of a diode–insulated gate bipolar transistor switch for modeling dc–dc converters is proposed. The formulated model has the form of a subcircuit for the simulation program with integrated circuits emphasis (SPICE) program and enables computations of both electrical characteristics of the converter and junction temperatures of the semiconductor devices. The equations used in the formulated model allow modeling the operation of the converter in both continuous conduction mode and discontinuous conduction mode. The correctness of the formulated model is experimentally verified for the boost converter. Good accuracy of modeling the characteristics of the converter is obtained. A.cir file based on the formulated model for an analysis in the SPICE program is attached to the article as the active content. [ABSTRACT FROM AUTHOR]

A quantum phase slip junction (QPSJ) based direct inverter gate is proposed in this article for the first time. The proposed inverter is simpler than the QPSJ-based inverter designed indirectly by using a two-input xor gate with specific input conditions. In the indirect inverter, one of the two inputs of the xor gate must be set to the clock pulse in order to perform the true inverter operation. However, the proposed direct inverter has only one input to which the input voltage can be applied directly, and therefore, it has a simpler structure with fewer elements that occupies less area on the chip. The true operation of the proposed inverter is verified by feeding its input with the standard pseudorandom binary sequence 3 and connecting its output to the next gate to test its ability for driving the next gate. [ABSTRACT FROM AUTHOR]

A GaN on engineered bulk silicon (GaN-on-EBUS) power IC platform featuring an industry-standard 200-V GaN power HEMT epi-structure has been recently demonstrated, showing effective isolation and crosstalk suppression between the high-side (HS) and low-side (LS) GaN transistors in half-bridge configuration. In this work, we scale up the vertical breakdown voltage (BV) of the GaN film on the EBUS substrate to be over 600 V. Meanwhile, the built-in back-to-back PN junctions along the trench created in the Si substrate are employed to provide an overvoltage-protection scheme through their intrinsic avalanche capability for the overlaying GaN half-bridge circuit. [ABSTRACT FROM AUTHOR]