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Background: Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbiosis. However, it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets. Results: In this study, we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score, and elevated levels of lipopolysaccharide (LPS) and D-lactate (D-LA) compared to healthy piglets. Subsequently, we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets. Diarrheal piglets demonstrated intestinal microbiota dysbiosis, characterized primarily by a higher Firmicutes to Bacteroidota ratio, a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri, and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron. Functional profiling of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data was performed, and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diarrhea. Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets. Furthermore, we explored the effects of dietary indole-3-aldehyde (IAld), a key tryptophan metabolite, on intestinal development and gut barrier function in weaned piglets. Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell (ISC) expansion in piglets. The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids. Conclusions: These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intestinal development by promoting ISC expansion in weaned piglets. [ABSTRACT FROM AUTHOR]

Simple Summary: Glycerol monolaurate, known for its strong antimicrobial properties, is a chemical compound formed by the combination of lauric acid and glycerol. This research focused on how glycerol monolaurate affects the growth, digestive enzyme activity, immune system, blood antioxidant level, and intestinal structure of black sea bream. This research could contribute to raising healthier aquatic animals, potentially reducing both the costs and environmental impact associated with aquaculture. These findings suggest that glycerol monolaurate may be the most suitable dietary supplement for fish; however, further research on its effect on the gut microbiota and gene expression is still required. An eight-week feeding trial was conducted to examine the impact of dietary supplementation with glycerol monolaurate (GML) on juvenile black sea bream. A basal diet was formulated containing 24% fish meal, while five additional diets were prepared, each supplemented with varying levels of GML: GML1 (0.01%), GML2 (0.02%), GML3 (0.04%), GML4 (0.08%), and GML5 (0.16%). Triplicate tanks were randomly allocated to each diet, each containing 20 fish with an initial weight of 1.55 ± 0.05 g. By the trial's end, the GML3 group displayed a notably higher final body weight (FBW), weight gain (WG), specific growth rate (SGR), and protein efficiency ratio (PER) compared to the other groups (p < 0.05), but the FCR was significantly higher in the control group. However, no significant differences were observed in the MFI, PPV, CF, HSI, IPF, VSI, or SR among the groups (p > 0.05). Regarding the proximate compositions of the dorsal muscle and whole body, no substantial differences were observed across the groups (p > 0.05). Additionally, there were no significant variations in digestive enzyme activity (p > 0.05), serum immune, or biochemical parameters in the midgut and hindgut among the treatment groups. But in the serum immune response IgM, C3 and C4 were significantly higher in the GML3 group as compared to the other groups (p < 0.05). However, the GML3 group exhibited significantly greater fore-intestinal villus height, crypt depth, villus height per crypt depth, and the number of goblet cells per villus compared to the other groups (p < 0.05). Overall, GML supplementation, particularly GML3, significantly improved growth indicators like the final body weight and intestinal morphology. While certain parameters remained unaffected, these findings suggest GML's potential as a beneficial dietary supplement in fish diets. [ABSTRACT FROM AUTHOR]

Meat goose can represent one of the most significant economic waterfowl in China. An excessively high stocking density can reduce the production performance and welfare of geese. Moreover, the light-emitting diode (LED) green light can play a pivotal role in regulating the growth and development of embryos and offspring in chickens. This study aims to determine the optimal regimen of LED green light in the high-density rearing mode of goslings. According to the actual production, a two-factor experiment was designed with the incubation light (dark and LED green) and growth light (LED white and LED green). A systematic analysis was made to explore the effects of LED green light on growth performance, intestinal development, blood hormones, feather quality, and pecking behaviors of geese in high-stocking density. The results show that the light intensities were 30–40 and 30–35 lx during the incubation and brooding period, respectively, with a light cycle of L:D = 24:0. The growth performance of geese was significantly improved by LED green light during the gosling brooding period. The LED green light during the growing period was found to significantly increase the body weight of geese at 14 days, as well as the daily gain and feed intake of geese for 1 to 14 days (P < 0.05). Furthermore, there was a significant decrease in the ratio of feed to gain of geese for 1 to 14 days (P < 0.05). A significant reduction was found in the concentration of serotonin and corticosterone in the blood, as well as the duodenal crypt depth of the geese at 14 days in the green light group during the growth period (P < 0.05), compared with the white light group. Furthermore, the LED green light irradiation during the growing period was observed to significantly increase the length of the chest and abdomen feathers of geese at 14 days post-hatching (P < 0.05), whereas, the back bare score of geese was reduced at 14 days post-hatching (P < 0.01), and the average pecking time and frequency of geese was reduced for 1 to 14 days post-hatching (P < 0.001). Thus, the green light environment during the growing period was significantly beneficial to the quality of the feathers and the development of geese plumage. The LED green light during the embryonic period was found to significantly reduce the average pecking time and frequency (P < 0.05) for better feather pecking behavior in geese. Moreover, the hatching procedure of green light outstandingly reduced the duodenal crypt depth (P < 0.01), where there was an increase in the jejunal villus height of geese at 14 days (P < 0.05), compared with the control group. The adverse effects of intensive farming were reduced on the intestinal development of geese. Moreover, there was no significant interaction to identify between the incubation and brood lights, in terms of the regulation of growth performance, intestinal development, blood hormones, feather quality (feather length, cleanliness, and damage degree), and feather pecking behavior of geese. Therefore, the LED green light shared the positive regulations on the negative effects of high-density rearing of goslings. Moreover, the LED green light during the growing period of goslings yielded superior outcomes. It is thus recommended that green light irradiation can be employed at the brood stage of goslings in actual production, in order to mitigate the adverse effects of modern intensive farming on goslings. The findings can provide a theoretical basis for the efficient production of geese. [ABSTRACT FROM AUTHOR]

The promotion of gut health, a pervasive problem in modern animal husbandry, positively affects organismal health, productivity, and economics. Porcine intestinal epithelial cells (IPEC-J2) continuously proliferate to maintain intestinal homeostasis, including barrier, immune, and absorptive functions. Gut homeostasis is fundamental to organismal health. ADP-ribosylation factor 1 (Arf1), a small GTPase, plays a crucial role in coordinating mTORC1 in response to nutrients, especially amino acid availability in the gut. mTORC1 is the central hub of proliferation. Thus, it seems likely that Arf1 promotes IPEC-J2 cell proliferation. However, the exact role of Arf1 in the porcine gut remains unclear. Therefore, we evaluated the functional role and possible mechanisms of Arf1 in the porcine intestine through Arf1 overexpression and knockdown in IPEC-J2 cells. Arf1 overexpression and knockdown significantly enhanced and inhibited, respectively, IPEC-J2 cell viability, and PCNA expression varied with Arf1 expression. Moreover, the proportion of Ki67-positive cells was significantly greater in the Arf1-overexpressing group than in the control group. These results suggest that Arf1 improves IPEC-J2 cell proliferation. The underlying mechanism was explored by Western blotting. Arf1 overexpression and knockdown significantly enhanced and suppressed, respectively, the levels of p-S6K1 and p-RPS6, which are key downstream targets of the mTORC1 signaling pathway. Collectively, our findings reveal the role of the Arf1-mTORC1 axis in IPEC-J2 cell proliferation and its potential function in regulating intestinal homeostasis and health. [ABSTRACT FROM AUTHOR]

The purpose of this experiment was to study the effects of alginic acid oligosaccharides (AOS) added to drinking water on the growth performance, carcass weight, immune organ index, intestinal morphology and small intestinal digestive enzyme activity of broilers. A total of 360 one-day-old Cobb 500 broilers were randomly divided into two groups, with 18 replicates in each group and ten broilers in each replicate. The control group was feed a basal diet, and AOS group was feed a basal diet and added 200 mg/L alginic acid oligosaccharides to drinking water from 1 to 21 days of age. The experimental period was 42 days. The results showed that compared with the control group, the average daily gain of broilers in AOS group was significantly increased (P<0.05), and the feed to gain ratio was significantly decreased from 1 to 21 days (P<0.05). During the whole experiment period, the average daily gain of broilers in the AOS group was significantly increased (P<0.05). Compared to the control group, the carcass weight of broilers in the AOS group was significantly increased at 21 and 42 days (P<0.05), as well as the spleen index and the bursa of Fabricius index at 21 days (P<0.05). The villus height of the small intestine in the AOS group broilers was significantly higher at 21 and 42 days, along with a significant increase in the villus-to-crypt ratio and intestinal lipase activity at 21 days (P<0.05). Studies show that adding the 200 mg/L alginic acid oligosaccharides group to drinking water can improve the growth performance, immunity, intestinal development and digestion of broilers, thereby improving the economic benefits of breeding. [ABSTRACT FROM AUTHOR]

Abstract Background Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbiosis. However, it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets. Results In this study, we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score, and elevated levels of lipopolysaccharide (LPS) and D-lactate (D-LA) compared to healthy piglets. Subsequently, we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets. Diarrheal piglets demonstrated intestinal microbiota dysbiosis, characterized primarily by a higher Firmicutes to Bacteroidota ratio, a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri, and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron. Functional profiling of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data was performed, and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diarrhea. Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets. Furthermore, we explored the effects of dietary indole-3-aldehyde (IAld), a key tryptophan metabolite, on intestinal development and gut barrier function in weaned piglets. Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell (ISC) expansion in piglets. The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids. Conclusions These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intestinal development by promoting ISC expansion in weaned piglets.

The effects of Lactobacillus plantarum in microencapsulation (LPM) on intestinal development in layer chicks were investigated in this study, as well as the colonization of L. plantarum in the gut. A total of 480 healthy Hy-Line Brown layer chicks at 0 d old were randomly divided into 4 groups (8 replicates each treatment), and the diets of these birds were supplemented with nothing (control), L. plantarum (0.02 g/kg feed; 109 CFU/kg feed), LPM (1.0 g/kg feed; 109 CFU/kg feed) and wall material of LPM (WM; 0.98 g/kg feed), respectively. Compared to control, LPM improved growth performance and intestinal development of layer chicks, evidenced by significantly increased body weight, average daily gain, average daily feed intake, villus height, villus height/crypt depth, as well as weight and length of the duodenum, jejunum and ileum (P < 0.05). These results could be attributed to the increased colonization of L. plantarum in the gut, which was verified by significant increases in lactic acid content, viable counts in chyme and mucosa (P < 0.05), as well as a visible rise in number of strains labeled with fluorescein isothiocyanate. Meanwhile, the relative abundances of Lactobacillus and Bifidobacterium significantly increased in response to microencapsulated L. plantarum supplementation (P < 0.05), accompanied by the significant up-regulation of colonization related genes (P < 0.05), encoding solute carrier family, monocarboxylate transporter, activin A receptor, succinate receptor and secretogranin II. To sum up, microencapsulated L. plantarum supplementation promoted intestinal development, which could be attributed to the enhancement of L. plantarum colonization in the intestine through the mutual assistance of Bifidobacterium and interactions with colonization related transmembrane proteins.

Necrotizing enterocolitis (NEC) is a complex, multifactorial gastrointestinal disorder predominantly affecting preterm infants. The pathogenesis of this condition involves a complex interplay between intestinal barrier dysfunction, microbial dysbiosis, and an altered immune response. This study investigates the potential role of endogenous hyaluronan (HA) in both the early phases of intestinal development and in the context of NEC-like intestinal injury. We treated neonatal CD-1 mouse pups with PEP1, a peptide inhibiting HA receptor interactions, from postnatal days 8 to 12. We evaluated postnatal intestinal developmental indicators, such as villi length, crypt depth, epithelial cell proliferation, crypt fission, and differentiation of goblet and Paneth cells, in PEP1-treated animals compared with those treated with scrambled peptide. PEP1 treatment significantly impaired intestinal development, as evidenced by reductions in villi length, crypt depth, and epithelial cell proliferation, along with a decrease in crypt fission activity. These deficits in PEP1-treated animals correlated with increased susceptibility to NEC-like injuries, including higher mortality rates, and worsened histological intestinal injury. These findings highlight the role of endogenous HA in supporting intestinal development and protecting against NEC. [ABSTRACT FROM AUTHOR]

BackgroundPrematurity or low birth weight (LBW), poses a significant challenge in global health. Exploring appropriate and effective nutritional interventions is crucial for the growth and development of LBW infants. Hydrolyzed protein formula has been suggested as a potential solution to prevent intestinal dysfunction and improve digestion and absorption in infants.ObjectivesThis study aimed to investigate the benefits of hydrolyzed protein formula on feeding intolerance, intestinal morphological development, and microbiota in a LBW piglet model.MethodsA total of 24 male piglets (3 d of age, 0.95–1.25 kg average BW) were assigned (8 pens/diet; 1 pigs/pen) into three dietary treatments and fed with a basic formula (BF), standard premature infant formula (SF) and hydrolyzed protein formula (HF) respectively, for 7 d. After the piglets sacrifice, growth performance, amino acid metabolism and intestinal morphology were assessed. 16S rRNA sequencing and microbial metabolic phenotypes analyzed the effects of different formula treatments on intestinal flora structure of LBW piglets.ResultsThe HF diet reduced the rates of diarrhea and milk vomiting were reduced by 20.44% (p > 0.05) and 58.44% (p > 0.05), and decreased the crypt depth in the ileum while increasing the ratio of villus height/crypt depth and the mRNA expressions of y+LAT1 and b0,+AT in the ileum (p

IntroductionThe objective of this study was to examine the impact of Codonopsis pilosula and Astragalus membranaceus extract (CA) on the growth performance, diarrhea rate, immune function, antioxidant capacity, gut microbiota, and short-chain fatty acids (SCFAs) in weaned piglets.MethodsA total of forty-eight 31-day-old weaned piglets, were divided into four groups randomly based on the treatment type: control group (CON), low dose group (LCA, 0.5% CA), medium dose group (MCA, 1.0% CA), and high dose group (HCA, 1.5% CA), and were fed for a duration of 28 days. On the morning of the 1st and 29th day, the piglets were assessed by weighing them on an empty stomach, recording their daily feed intake and diarrhea rate.ResultsCA increased the average daily weight gain and reduced F/G without significant differences, and the diarrhea rate was reduced in the LCA and MCA groups. Furthermore, the levels of T-AOC, SOD, GSH-Px, and MDA were increased. The levels of T-AOC in the LCA group and the MCA group, SOD in the MCA group, and GSH-Px in the HCA group were significantly higher compared with the CON group (p < 0.05). Additionally, CA significantly increased IgM, IgG, and IgA levels (p < 0.05). The results of gut microbiota analysis showed that the bacterial population and diversity of faeces were changed with the addition of CA to basal diets. CA increased the abundance of the beneficial bacterial Firmicutes and Lactobacillus. Additionally, Compared with the CON group, CA significantly increased the SCFAs content of weaned piglets (p < 0.05).DiscussionCA can alleviate oxidative stress, improve immunity and antioxidant capacity, increase the abundance of beneficial bacteria, and the content of SCFAs for improving the intestinal barrier of piglets, thus promoting growth and reducing diarrhea rate in weaned piglets.

Early weaning-induced stress precipitates diarrhoea, significantly curtailing the growth performance of piglets. A pivotal contributor to this postweaning affliction is the emergence of gut bacterial dysbiosis. Enterococcus hirae, a promising probiotic, has indicated unclear effects and mechanisms on intestinal health. In this study, we investigated the effects and underlying mechanisms of oral supplementation with Ningxiang pig-derived Enterococcus hirae HNAU0516 orally supplementation on the gut bacterial community, immune response and gut barrier function in piglets. 21 d age Duroc × (Landrace × Yorkshire) piglets with a similar BW were randomly allocated to two groups. The Enterococcus hirae HNAU0516 administration group was inoculated orally with Ningxiang pig-derived Enterococcus hirae HNAU0516 throughout the trial period. Conversely, the control group received the same volume of physiological saline. Our findings revealed that Enterococcus hirae HNAU0516 supplementation effectively reduced diarrhoea rates of piglets (P = 0.010). Notably, this probiotic promoted intestinal development and enhanced intestinal barrier function. It also showed potential anti-inflammatory properties. Furthermore, Enterococcus hirae HNAU0516 supplementation significantly remodelled the colonic microbiota and increased the production of acetate (P = 0.007). In conclusion, our study highlights that Ningxiang pig-derived Enterococcus hirae HNAU0516 improves postweaning diarrhoea by promoting intestinal development, enhancing intestinal barrier function, decreasing intestinal permeability, modulating intestinal microbiota, and increasing short-chain fatty acids production.

ABSTRACT: Metabolic disorders in maternal generation during the late egg-laying period have adverse effects on neonatal development. The study was conducted to clarify the effects of maternal feeding of hawthorn-leaf flavonoid (HF) on the microbial community and intestinal development of chicks. Breeder hens were fed a basic corn-soybean diet, while the treatment groups were supplemented with 30 or 60 mg/kg HF. The offspring chicks were divided into CON, LHF, and HHF groups according to the maternal treatments. Maternal HF supplementation at 60 mg/kg increased the average daily gain and decreased the feed conversion rate of chicks (P < 0.05), but did not affect the average daily feed intake. HF treatments increased the villus height to crypt depth ratio and up-regulated the protein expressions of PCNA, IGF-1R, PI3K and p-mTOR in the jejunum (P < 0.05) of 1-day-old and 14-day-old chicks. Additionally, maternal HF treatment up-regulated the mRNA expression of tight junction transmembrane proteins (occludin) and scaffolding proteins (ZO-1 and ZO-2) in the jejunum of 1-day-old chicks (P < 0.05). Moreover, the maternal effects of HF on ZO-1 expression could last for 14 d (P < 0.05). Interestingly, dietary HF supplementation altered the vertically transmitted microbial community from breeder hens to chicks, especially increased the relative abundance of probiotics (i.e., Clostridium_sensu_stricto_1) in the meconium of chicks (P < 0.05), which may help with early gut microbiota colonization and intestinal development. In summary, dietary HF supplementation for breeder hens altered the bacterial community of neonates and might promote intestinal development of chicks through the IGF-1R/AKT/mTOR signaling pathway.

Methionine (Met) is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and growth. The present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and health. Fertilized eggs of the two genotypes, TETRA-SL layer hybrid (TSL) — commercial layer hybrid and Hungarian Partridge colored hen breed (HPC) — a native genotype, were randomly distributed into four treatments for each genotype. The treatment groups include the following: 1) control non-injected eggs (NoIn); 2) saline-injected (SaIn); 3) DL-Met injected (DLM); and 4) L-Met injected (LM). The in ovo injection was carried out on 17.5 d of embryonic development; after hatching, eight chicks per group were sacrificed, and the jejunum was extracted for analysis. The results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width, villus height, and villus area (P

The purpose of this experiment was to study the effects of dietary arginine level on the growth performance, immune function, and small intestine development of broilers. A total of 600 one-day-old male Arbor Acres broilers were randomly divided into five groups with six replicates in each group and 20 chickens in each replicate. Each group was supplemented with 0, 0.65%, 1.15%, 1.65%, and 2.15% arginine in the basal diet. The trial period was 42 days. The results showed that compared with the control group, the body weight and average daily gain (ADG) of broilers in the 0.65% supplementation group were significantly higher (P<0.05), and the villus height/recess depth of duodenum and jejunum were significantly higher after 42 days of experiment (P<0.05). Compared with the control group, the content of serum immune protein in the 2.15% supplementation group increased significantly on the 21st day (P<0.05), and the index of bursa of fabricius increased significantly on the 42st day (P<0.05). The study indicates that proper addition of 0.65% arginine in basal diet can increase the body weight, average daily gain, and promote the development of duodenum and jejunum in the later growth stage of broilers. High level of arginine (2.15%) can improve the level of serum immune protein and promote the development of small intestine. [ABSTRACT FROM AUTHOR]

The experiment was to study the effects of dietary isochlorogenic acid (ICGA) on early growth performance, intestinal structure, immune performance, and microbial diversity of broilers. 400 one-day-old Kebao broilers, half in male and half female, were randomly divided into five groups with eight replicates in each group and 10 chickens in each replicate. Each group was fed with basal diet (control group), basal diet + 500 mg/kg ICGA (T1 group), basal diet + 1 000 mg/kg ICGA (T2 group), basal diet + 2 000 mg/kg ICGA (T3 group), and basal diet + 3 000 mg/kg ICGA (T4 group). The trial period was 21 days. The results showed that the average daily feed intake (ADFI), average daily gain (ADG), and feed-to-weight ratio (F/G) were not significantly different among the groups (P>0.05). The apparent metabolic rate of crude protein (CP) in T3 group and T4 group was significantly higher than that in control group and T2 group (P<0.05), and the apparent metabolic rate of calcium (Ca) in all experimental groups was extremely higher than that in the control group (P<0.01). The ileal pH value of T3 group was significantly lower than that of other groups (P<0.05). Serum complement C3 content of broilers in each experimental group was significantly higher than that in the control group (P<0.05), and serum immunoglobulin G (IgG) and immunoglobulin M (IgM) content of broilers in T3 group were significantly higher than that in other groups (P<0.05). The villus height of T3 group was significantly higher than that of the control group and T1 group (P<0.05), and the villus-saphenous ratio of T3 group was significantly higher than that of the control group, T1 group, and T2 group (P<0.05). Subdoligranulum&lowbar;variabile in T2 group and T3 group was significantly higher than that in control group and T1 group (P<0.05). Subdoligranulum&lowbar;variabile in T2 group was extremely higher than that in the control group (P<0.01). The study indicates that the nutrient apparent metabolic rate, immune performance, intestinal structure, and intestinal flora were improved by adding ICGA in the diet. The appropriate level of ICGA in the diet of broilers is 2 000 mg/kg. [ABSTRACT FROM AUTHOR]

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Simple Summary: This study aims to investigate the effects of maternal dietary supplementation with ornithine on productive performance and placental angiogenesis of sows. We found that maternal dietary supplementation with 0.15% ornithine increased the average birth weight of piglets and intestinal development of suckling piglets. In addition, Orn facilitated placental angiogenesis by regulating vascular endothelial growth factor-A (VEGF-A). These results indicated that dietary supplementation with 0.15% ornithine is an effective strategy to improve the productive performance of sows. The blood vessels of the placenta are crucial for fetal growth. Here, lower vessel density and ornithine (Orn) content were observed in placentae for low-birth-weight fetuses versus normal-birth-weight fetuses at day 75 of gestation. Furthermore, the Orn content in placentae decreased from day 75 to 110 of gestation. To investigate the role of Orn in placental angiogenesis, 48 gilts (Bama pig) were allocated into four groups. The gilts in the control group were fed a basal diet (CON group), while those in the experimental groups were fed a basal diet supplemented with 0.05% Orn (0.05% Orn group), 0.10% Orn (0.10% Orn group), and 0.15% Orn (0.15% Orn group), respectively. The results showed that 0.15% Orn and 0.10% Orn groups exhibited increased birth weight of piglets compared with the CON group. Moreover, the 0.15% Orn group was higher than the CON group in the blood vessel densities of placenta. Mechanistically, Orn facilitated placental angiogenesis by regulating vascular endothelial growth factor-A (VEGF-A). Furthermore, maternal supplementation with 0.15% Orn during gestation increased the jejunal and ileal villi height and the concentrations of colonic propionate and butyrate in suckling piglets. Collectively, these results showed that maternal supplementation with Orn promotes placental angiogenesis and improves intestinal development of suckling piglets. [ABSTRACT FROM AUTHOR]

Abstract Backgrounds The intestinal development in early life is profoundly influenced by multiple biological components of breast milk, in which milk-derived extracellular vesicles (mEVs) contain a large amount of vertically transmitted signal from the mother. However, little is known about how maternal fiber-rich diet regulates offspring intestinal development by influencing the mEVs. Results In this study, we found that maternal resistant starch (RS) consumption during late gestation and lactation improved the growth and intestinal health of offspring. The mEVs in breast milk are the primary factor driving these beneficial effects, especially enhancing intestinal cell proliferation and migration. To be specific, administration of mEVs after maternal RS intake enhanced intestinal cell proliferation and migration in vivo (performed in mice model and indicated by intestinal histological observation, EdU assay, and the quantification of cyclin proteins) and in vitro (indicated by CCK8, MTT, EdU, and wound healing experiments). Noteworthily, miR-146a-5p was found to be highly expressed in the mEVs from maternal RS group, which also promotes intestinal cell proliferation in cells and mice models. Mechanically, miR-146a-5p target to silence the expression of ubiquitin ligase 3 gene NEDD4L, thereby inhibiting DVL2 ubiquitination, activating the Wnt pathway, and promoting intestinal development. Conclusion These findings demonstrated the beneficial role of mEVs in the connection between maternal fiber rich diet and offspring intestinal growth. In addition, we identified a novel miRNA-146a-5p-NEDD4L-β-catenin/Wnt signaling axis in regulating early intestinal development. This work provided a new perspective for studying the influence of maternal diet on offspring development. Graphical Abstract

Background and Aims: Necrotizing enterocolitis is a severe gastrointestinal complication of prematurity. Using small intestinal organoids derived from fetal tissue of a gestational age similar to an extremely preterm infant, this study aims to assess the effect of diet on intestinal epithelial growth and differentiation to elucidate the role nutrition type plays in intestinal development and modifies the risk for necrotizing enterocolitis. Methods: Organoids were cultured for 5 days in growth media and 5 days in differentiation media supplemented 1:40 with 4 different diets: parental milk, donor human milk, standard formula, or extensively hydrolyzed formula. Images were captured daily and organoids were quantified. Organoids were preserved for RNA sequencing and immunofluorescence staining with Ki67, cleaved caspase 3, and chromogranin-A. Media was saved for cytokine/chemokine and growth factor analysis. Results: Human milk supplementation improved growth and differentiation of intestinal organoids generating larger organoids during the growth phase and organoids with longer and wider buds during differentiation compared to formula. Ki67 staining confirmed the proliferative nature of milk-supplemented organoids and chromogranin A staining proved that MM-supplemented organoids induced highest enteroendocrine differentiation. Human milk supplementation also upregulated genes involved in Wnt signaling and fatty acid metabolism pathways and promoted a homeostatic immune landscape, including via increased secretion of tumor necrosis factor-related apoptosis-inducing ligand among other cytokines. Conversely, organoids supplemented with formula had a downregulation of cell-cycle-promoting genes and a more inflammatory immune signature, including a reduced level of leukemia inhibitory factor. Conclusion: Our results demonstrate that parental milk, and to a lesser extent donor human milk, support robust intestinal epithelial proliferation, differentiation, and homeostatic cytokine production, suggesting a critical role for factors enriched in human milk in intestinal epithelial health.

An eight-week feeding trial was conducted to examine the impact of dietary supplementation with glycerol monolaurate (GML) on juvenile black sea bream. A basal diet was formulated containing 24% fish meal, while five additional diets were prepared, each supplemented with varying levels of GML: GML1 (0.01%), GML2 (0.02%), GML3 (0.04%), GML4 (0.08%), and GML5 (0.16%). Triplicate tanks were randomly allocated to each diet, each containing 20 fish with an initial weight of 1.55 ± 0.05 g. By the trial’s end, the GML3 group displayed a notably higher final body weight (FBW), weight gain (WG), specific growth rate (SGR), and protein efficiency ratio (PER) compared to the other groups (p < 0.05), but the FCR was significantly higher in the control group. However, no significant differences were observed in the MFI, PPV, CF, HSI, IPF, VSI, or SR among the groups (p > 0.05). Regarding the proximate compositions of the dorsal muscle and whole body, no substantial differences were observed across the groups (p > 0.05). Additionally, there were no significant variations in digestive enzyme activity (p > 0.05), serum immune, or biochemical parameters in the midgut and hindgut among the treatment groups. But in the serum immune response IgM, C3 and C4 were significantly higher in the GML3 group as compared to the other groups (p < 0.05). However, the GML3 group exhibited significantly greater fore-intestinal villus height, crypt depth, villus height per crypt depth, and the number of goblet cells per villus compared to the other groups (p < 0.05). Overall, GML supplementation, particularly GML3, significantly improved growth indicators like the final body weight and intestinal morphology. While certain parameters remained unaffected, these findings suggest GML’s potential as a beneficial dietary supplement in fish diets.

Backgrounds: The intestinal development in early life is profoundly influenced by multiple biological components of breast milk, in which milk-derived extracellular vesicles (mEVs) contain a large amount of vertically transmitted signal from the mother. However, little is known about how maternal fiber-rich diet regulates offspring intestinal development by influencing the mEVs. Results: In this study, we found that maternal resistant starch (RS) consumption during late gestation and lactation improved the growth and intestinal health of offspring. The mEVs in breast milk are the primary factor driving these beneficial effects, especially enhancing intestinal cell proliferation and migration. To be specific, administration of mEVs after maternal RS intake enhanced intestinal cell proliferation and migration in vivo (performed in mice model and indicated by intestinal histological observation, EdU assay, and the quantification of cyclin proteins) and in vitro (indicated by CCK8, MTT, EdU, and wound healing experiments). Noteworthily, miR-146a-5p was found to be highly expressed in the mEVs from maternal RS group, which also promotes intestinal cell proliferation in cells and mice models. Mechanically, miR-146a-5p target to silence the expression of ubiquitin ligase 3 gene NEDD4L, thereby inhibiting DVL2 ubiquitination, activating the Wnt pathway, and promoting intestinal development. Conclusion: These findings demonstrated the beneficial role of mEVs in the connection between maternal fiber rich diet and offspring intestinal growth. In addition, we identified a novel miRNA-146a-5p-NEDD4L-β-catenin/Wnt signaling axis in regulating early intestinal development. This work provided a new perspective for studying the influence of maternal diet on offspring development. [ABSTRACT FROM AUTHOR]

[Objectives]The experiment of this study was conducted to investigate the characteristics of fiber tolerance(assessed by apparent fiber digestibility), intestinal development and intestinal microorganism of Suhuai finishing pigs of different months of age, and to analyze the correlation between intestinal development, fiber apparent digestibility and intestinal microorganism. [Methods]Eighty-two Suhuai finishing pigs with similar birth conditions were selected and raised in the same feeding environment. The pigs were slaughtered at 7 or 8 months of age, and the contents of posterior colon samples were collected to determine the apparent digestibility of various nutrients in posterior colon segment at different months of age. The intestinal length and circumference were measured to analyze the intestinal development differences of Suhuai finishing pigs at different months of age. The correlations between apparent digestibility and intestinal length and circumference were also analyzed. And according to the apparent digestibility of fiber in the posterior segment of colon, 5 pigs in the high fiber digestibility group and 5 pigs in the low fiber digestibility group were selected at 7 and 8 months of age to analyze the abundance of common fibrolytic bacteria in the cecum and colon contents. [Results]The apparent digestibility of crude fiber(CF), neutral detergent fiber(NDF), acid detergent fiber(ADF), crude protein(CP)and ether extract(EE)of 8 months Suhuai pigs were significantly higher than those of 7 months Suhuai pigs(P<0.05). There was no significant difference in the length of large intestine, small intestine and whole intestine, cecum and colon circumference between Suhuai pigs at 7 and 8 months of age. There was a significant positive correlation between cecum circumference and apparent digestibility of CF, ADF and EE(P<0.05). There was no significant difference in the abundance of Ruminococcus albus and Clostridium leptum in cecum and colon of Suhuai pigs at 7 and 8 months of age between high and low fiber digestibility groups. The abundance of Clostridium leptum in colon of Suhuai pigs aged 7 months was significantly higher than that in cecum in both high and low fiber digestibility groups(P<0.05). The abundance of Clostridium leptum in colon of 8-month-old Suhuai pigs in low fiber digestibility group was significantly lower than that of 7-month-old Suhuai pigs(P<0.05). [Conclusions]Different months age of Suhuai pigs had different characteristics of fiber tolerance. The digestive performance of Suhuai pigs improved with age, the nutrients apparent digestibility of Suhuai pigs aged 8 months was significantly higher than that of 7 months of age, and the fiber digestibility of Suhuai pigs was significantly positively correlated with cecum circumference. But the Ruminococcus albus and Clostridium leptum in intestinal contents might not be the key microorganisms affecting the fiber digestion of Suhuai pig. [ABSTRACT FROM AUTHOR]

The alleviating effects of Lactobacillus plantarum in microencapsulation (LPM) on lipopolysaccharide (LPS)-induced intestinal inflammatory injury were investigated in layer chicks. A total of 252 healthy Hy-Line Brown layer chicks were randomly divided into six groups. Birds were injected with saline or LPS except for the control, and the diets of birds subjected to LPS were supplemented with nothing, L. plantarum, LPM, and wall material of LPM, respectively. The viable counts of LPM reached 109 CFU/g, and the supplemental levels of L. plantarum, LPM, and WM were 0.02 g (109 CFU), 1.0 g, and 0.98 g, per kilogram feed, respectively. LPS administration caused intestinal damage in layer chicks, evidenced by increased proinflammatory factors accompanied by poor intestinal development and morphology (p < 0.05). LPM/LPS significantly increased body weight, small intestine weight and length, villus height, villus height/crypt depth, and mRNA relative expression of tight junction protein genes (p < 0.05) and performed better than free L. plantarum. These findings could be attributed to the significant increase in viable counts of L. plantarum in the small intestine (p < 0.05), as well as the enhanced levels of Actinobacteriota, Lactobacillaceae, and Lactobacillus in intestinal microbiota (p < 0.05). Such results could further significantly increase goblet and PCNA+ cell percentage (p < 0.05); the mRNA relative expressions of epithelial cell, fast-cycling stem cell, quiescent stem cell, endocrine cell, and Paneth cell; and goblet and proliferative cell marker genes, including E-cadherin, Lgr-5, Bmi-1, ChA, Lysozome, Mucin-2, and PCNA (p < 0.05). Furthermore, the mRNA relative expressions of key genes involved in epithelial cell proliferation, namely, c-Myc, Cyclin-1, Wnt-3, Lrp-5, and Olfm-4, exhibited significant upregulation compared with the LPS treatment, as well as the differentiating genes Notch-1 and Hes-1 (p < 0.05). To sum up, microencapsulated L. plantarum supplementation could alleviate intestinal injury in layer chicks induced by LPS by promoting the proliferation and differentiation of intestinal epithelial cells, which could be attributed to the increase in viable count of L. plantarum in the gut and optimization in intestinal microbial flora. [ABSTRACT FROM AUTHOR]

ABSTRACT: The health benefits of nutritional interventions targeting the gut microbiota in early life are transient, such as probiotics, prebiotics, and synbiotics. This study sought to determine whether supplementation with Bifidobacterium infantis 79 (B79), 2′-fucosyllactose (2′-FL), or both (B79+2′FL) would lead to persistent health benefits in neonatal BALB/c mice. We found that at postnatal day (PND) 21, Ki67 and MUC2 expression increased, while total serum IgE content decreased in the B79, 2′-FL, and B79+2′-FL groups. The gut microbiota structure and composition altered as well. The levels of propionic acid, sIgA, and IL-10 increased in the 2′-FL group. Moreover, butyric acid content increased, while IL-6, IL-12p40, and tumor necrosis factor-α decreased in the B79+2′-FL group. At PND 56, Ki67 and MUC2 expression increased, whereas the gut microbiota remained altered in all 3 groups. The serum total IgG level increased only in the B79+2′-FL group. In conclusion, our study suggests that early-life supplementation with B79, 2′-FL, or their combination persistently alters the gut microbiome and promotes intestinal development; the immunomodulatory capacity of B79 and 2'-FL occurs during weaning, and their combination may persist into adulthood.

Increasing evidence suggests that prolonged antibiotic therapy in preterm infants is associated with increased mortality and morbidities, such as necrotizing enterocolitis (NEC), a devastating gastrointestinal pathology characterized by intestinal inflammation and necrosis. While a clinical correlation exists between antibiotic use and the development of NEC, the potential causality of antibiotics in NEC development has not yet been demonstrated. Here, we tested the effects of systemic standard-of-care antibiotic therapy for ten days on intestinal development in neonatal mice. Systemic antibiotic treatment impaired the intestinal development by reducing intestinal cell proliferation, villi height, crypt depth, and goblet and Paneth cell numbers. Oral bacterial challenge in pups who received antibiotics resulted in NEC-like intestinal injury in more than half the pups, likely due to a reduction in mucous-producing cells affecting microbial-epithelial interactions. These data support a novel mechanism that could explain why preterm infants exposed to prolonged antibiotics after birth have a higher incidence of NEC and other gastrointestinal disorders.

Necrotizing enterocolitis (NEC) is a complex, multifactorial gastrointestinal disorder predominantly affecting preterm infants. The pathogenesis of this condition involves a complex interplay between intestinal barrier dysfunction, microbial dysbiosis, and an altered immune response. This study investigates the potential role of endogenous hyaluronan (HA) in both the early phases of intestinal development and in the context of NEC-like intestinal injury. We treated neonatal CD-1 mouse pups with PEP1, a peptide inhibiting HA receptor interactions, from postnatal days 8 to 12. We evaluated postnatal intestinal developmental indicators, such as villi length, crypt depth, epithelial cell proliferation, crypt fission, and differentiation of goblet and Paneth cells, in PEP1-treated animals compared with those treated with scrambled peptide. PEP1 treatment significantly impaired intestinal development, as evidenced by reductions in villi length, crypt depth, and epithelial cell proliferation, along with a decrease in crypt fission activity. These deficits in PEP1-treated animals correlated with increased susceptibility to NEC-like injuries, including higher mortality rates, and worsened histological intestinal injury. These findings highlight the role of endogenous HA in supporting intestinal development and protecting against NEC.

ABSTRACT: Early embryonic exogenous feeding of bioactive substances is a topic of interest in poultry production, potentially improving gastrointestinal tract (GIT) development, stimulating immunization, and maximizing the protection capability of newly hatched chicks. However, the biophysiological actions and effects of in ovo administered bioactive substances are inconsistent or not fully understood. Thus, this paper summarizes the functional effects of bioactive substances and their interaction merits to augment GIT development, the immune system, and microbial homeostasis in newly hatched chicks. Prebiotics, probiotics, and synbiotics are potential bioactive substances that have been administered in embryonic eggs. Their biological effects are enhanced by a variety of mechanisms, including the production of antimicrobial peptides and antibiotic responses, regulation of T lymphocyte numbers and immune-related genes in either up- or downregulation fashion, and enhancement of macrophage phagocytic capacity. These actions occur directly through the interaction with immune cell receptors, stimulation of endocytosis, and phagocytosis. The underlying mechanisms of bioactive substance activity are multifaceted, enhancing GIT development, and improving both the innate and adaptive immune systems. Thus summarizing these modes of action of prebiotics, probiotics and synbiotics can result in more informed decisions and also provides baseline for further research.

The health benefits of nutritional interventions targeting the gut microbiota in early life are transient, such as probiotics, prebiotics, and synbiotics. This study sought to determine whether supplementation with Bifidobacterium infantis 79 (B79), 2′-fucosyllactose (2′-FL), or both (B79+2′FL) would lead to persistent health benefits in neonatal BALB/c mice. We found that at postnatal day (PND) 21, Ki67 and MUC2 expression increased, while total serum IgE content decreased in the B79, 2′-FL, and B79+2′-FL groups. The gut microbiota structure and composition altered as well. The levels of propionic acid, sIgA, and IL-10 increased in the 2′-FL group. Moreover, butyric acid content increased, while IL-6, IL-12p40, and tumor necrosis factor-α decreased in the B79+2′-FL group. At PND 56, Ki67 and MUC2 expression increased, whereas the gut microbiota remained altered in all 3 groups. The serum total IgG level increased only in the B79+2′-FL group. In conclusion, our study suggests that early-life supplementation with B79, 2′-FL, or their combination persistently alters the gut microbiome and promotes intestinal development; the immunomodulatory capacity of B79 and 2'-FL occurs during weaning, and their combination may persist into adulthood. [Display omitted] [ABSTRACT FROM AUTHOR]

Simple Summary: Bile acids have various physiological functions in the body. On the one hand, they can promote the digestion and absorption of fats as emulsifiers, and on the other hand, they participate in many metabolic pathways as signaling molecules. In addition, bile acids can shape the gut flora and improve gut health. This experiment aimed to investigate the effects of non-12α-hydroxylated bile acids on the growth and development, and intestinal flora of weaned piglets. This study showed that the addition of non-12α-hydroxylated bile acids could improve growth performance and nutrient digestibility in weaned piglets without negatively affecting the liver. In addition, the addition of non-12α-hydroxylated bile acids also improved the intestinal flora, increased the abundance of Lactobacillus_johnsonii, and increased the content of short-chain fatty acids. As an emulsifier and bioactive substance, bile acids (BAs) participate in the absorption of nutrients and in various physiological processes. The objective of this experiment was to investigate the effects of non-12α-hydroxylated BAs (including hyocholic acid, hyodeoxycholic acid and chenodeoxycholic acid, from now on referred to as NBAs) on growth performance, BAs metabolism and the intestinal flora of piglets. The experiment included four groups, with eight piglets per group. The four groups of pigs were fed 0, 60, 120 and 180 mg/kg of NBAs, respectively. The results show that adding NBAs significantly increased the final weight (FW), average daily feed intake (ADFI), average daily gain (ADG), and digestibility of crude fat (EE) and organic matter (OM) in piglets (p < 0.05). Adding NBAs significantly increased the villus height (VH) of the jejunum and ileum (p < 0.05). In addition, NBAs supplementation increased the content of urea nitrogen (BUN) and creatinine (CREA) as well as the ratio of urea nitrogen to creatinine (BUN/CREA) in serum (p < 0.05). Adding NBAs can affect the genes related to BAs enterohepatic circulation. Specifically, adding NBAs significantly decreased the relative mRNA abundance of FXR in the liver (p < 0.05), significantly increased the relative mRNA abundance of CYP27A1 (p < 0.05), and significantly increased the relative mRNA abundance of NTCP (p < 0.05). Adding NBAs also significantly decreased the relative mRNA abundance of FXR in the ileum (p < 0.05). In the full-length 16S rDNA sequencing analysis, ten biomarkers were found from the gate to the species level. NBAs mainly enriched Lactobacillus_Johnsonii and decreased the abundance of Streptococcus_alactolyticus. Short-chain fatty acids (SCFAs) content in the colon was significantly increased (p < 0.05). These results indicate that NBAs supplementation can improve the growth performance of piglets, promote the development of the bile acid replacement pathway and improve intestinal flora. [ABSTRACT FROM AUTHOR]

Methionine is important for intestinal development and homeostasis in various organisms. However, the underlying mechanisms are poorly understood. Here, we demonstrate that the methionine adenosyltransferase gene Mat2a is essential for intestinal development and that the metabolite S-adenosyl-L-methionine (SAM) plays an important role in intestinal homeostasis. Intestinal epithelial cell (IEC)-specific knockout of Mat2a exhibits impaired intestinal development and neonatal lethality. Mat2a deletion in the adult intestine reduces cell proliferation and triggers IEC apoptosis, leading to severe intestinal epithelial atrophy and intestinal inflammation. Mechanistically, we reveal that SAM maintains the integrity of differentiated epithelium and protects IECs from apoptosis by suppressing the expression of caspases 3 and 8 and their activation. SAM supplementation improves the defective intestinal epithelium and reduces inflammatory infiltration sequentially. In conclusion, our study demonstrates that methionine metabolism and its intermediate metabolite SAM play essential roles in intestinal development and homeostasis in mice. [ABSTRACT FROM AUTHOR]

Single-cell transcriptomic analyses have revealed an unexpectedly high heterogeneity in the identity and function of intestinal fibroblasts. Research from the past few years has provided compelling evidence that intestinal fibroblasts act as a niche not only for intestinal epithelial stem cells, but also for lymphatic endothelial, blood endothelial, and immune cells. Intestinal fibroblasts are a promising, yet unexplored, target for novel therapeutic interventions to combat intestinal disease. Only in recent years have we begun to appreciate the involvement of fibroblasts in intestinal development, tissue homeostasis, and disease. These insights followed the advent of single-cell transcriptomics that allowed researchers to explore the heterogeneity of intestinal fibroblasts in unprecedented detail. Since researchers often defined cell types and their associated function based on the biological process they studied, there are a plethora of partially overlapping markers for different intestinal fibroblast populations. This ambiguity complicates putting different research findings into context. Here, we provide a census on the function and identity of intestinal fibroblasts in mouse and human. We propose a simplified framework consisting of three colonic and four small intestinal fibroblast populations to aid navigating the diversity of intestinal fibroblasts. [ABSTRACT FROM AUTHOR]

To investigate the effects of Gamma-aminobutyric (GABA) acid and IAB on the growth performance of broilers, two broiler experiments were conducted separately. The first batch of broiler experiments was experiment 1, in which 280 broiler chickens were randomly selected and divided into two groups, including the control group and the GABA+IAB experimental group, respectively, with four replicates in each group and 35 chickens in each replicates. The second batch of broiler experiment was experiment 2, in which 192 broiler chickens were randomly selected and divided into four groups, including the control group, GABA experimental group, IAB experimental group, and GABA+IAB experimental group, with four replicates in each group, each replicates had 12 chickens, during the experiment, the daily weight gain, daily feed consumption, feed to meat ratio, immune organ index, and villus length of the broiler chickens in the experimental group were statistically analyzed. The results were as follows: ① the feed meat of GABA+IAB test group was 5.42% lower than that of the control group, and the EPI was 6.28% higher than that of the control group. ② The jejunal villus height and the ratio of villus to crypt of IAB test group and GABA+IAB test group were higher than that of the control group and GABA test group. ③ The spleen index of GABA test group, IAB test group and GABA+IAB test group were significantly higher than that of the control group. The results indicate that adding GABA and IAB to feed can significantly improve the production performance of broilers, while also improving the immune organ index. Among them, IAB has the best promoting effect on intestinal development in broilers, and the combined application of GABA and IAB has a higher effect than single use. [ABSTRACT FROM AUTHOR]

Congenital tufting enteropathy (CTE) is a life-threatening intestinal disorder resulting from loss-of-function mutations in EPCAM and SPINT2. Mice deficient in Spint2, encoding the protease inhibitor HAI-2, develop CTE-like intestinal failure associated with a progressive loss of the EpCAM protein, which is caused by unchecked activity of the serine protease matriptase (ST14). Here, we show that loss of HAI-2 leads to increased proteolytic processing of EpCAM. Elimination of the reported matriptase cleavage site strongly suppressed proteolytic processing of EpCAM in vitro and in vivo. Unexpectedly, expression of cleavage-resistant EpCAM failed to prevent intestinal failure and postnatal lethality in Spint2-deficient mice. In addition, genetic inactivation of intestinal matriptase (St14) counteracted the effect of Spint2 deficiency in mice expressing cleavage-resistant EpCAM, indicating that matriptase does not drive intestinal dysfunction by excessive proteolysis of EpCAM. Interestingly, mice expressing cleavageresistant EpCAM developed late-onset intestinal defects and exhibited a shortened lifespan even in the presence of HAI-2, suggesting that EpCAM cleavage is indispensable for EpCAM function. Our findings provide new insights into the role of EpCAM and the etiology of the enteropathies driven by Spint2 deficiency. [ABSTRACT FROM AUTHOR]

[Objectives] The paper aimed to investigate the effects of early incubation temperature on yolk sac absorption and intestinal development of chicks [Methods] A total of 189 one-day-old healthy Huainan Ephedra chicks were randomly divided into 3 groups with 3 replicates in each group and 21 chicks in each replicate: 33°C group, 30 °C group and 27 °C group, respectively (After 2.5 d, the brood temperature of the three groups was uniform). At 0.5,1,1.5,2,2.5,7, 14 days old, 3 rats were collected from each replicate. After weighing, blood was collected from the heart for serum separation for antibody detection, and yolk sacs were collected for fatty acid composition analysis (within 2.5 d) at slaughter. Sections of small intestine were taken for fluorescence quantitative analysis of metabolity-related gene expression levels. [Results] The body weight of chicks at 14 days old in 30 °C group was significantly higher than those in the other two groups (P<0.05), and there was no significant difference in yolk sac weight between different temperature groups except for incubation for 1.5 d(36 h). Yolk sac fatty acids were mainly composed of C16:0,C18: In9t, C18:0 and C18:2n6t, and there was no significant difference in fatty acid content among all groups (P>0.05). The spleen weight of 7 days old chicks in 30 °C group was significantly higher than those in the other two groups (P<0.05), and serum IgM and IgY were significantly higher than those in 33 °C group(P<0.05). At 14 days old, the ratio of villi to crypt to duodenum and jejunum in 30 °C group was significantly higher than those in 33 °C group, but the ratio of villi to crypt to ileum in 30 °C group was significantly lower than those in 27 °C and 33 C groups (P<0.05). The expression levels of GLUUT2 and Pep71 in ileum of chicks in 30 °C group were significantly higher than those in 27 °C group(P<0.05). [Conclusions] The temperature at the early stage of incubation had significant effects on the growth and development of chicks. Incubation at 33 °C was beneficial to the absorption of yolk sac in the first 1.5 d, and incubation at 30 °C was beneficial to the increase of body weight, small intestinal morphological development, immune level establishment and the expression of genes related to intestinal nutrient absorption. [ABSTRACT FROM AUTHOR]

Abstract Background Nutrition drives immunity and health in animals, and maternal immunity benefits offspring. In our previous study, a nutritional intervention strategy was found to promote the immunity of hens, which subsequently improved immunity and growth in offspring chicks. Maternal effects clearly exist, but how are mothers’ immune advantages transferred to their offspring, and how do they benefit them? Results Here, we traced the beneficial effects back to the process of egg formation in the reproductive system, and we focused on the embryonic intestinal transcriptome and development, as well as on maternal microbial transfer in offspring. We found that maternal nutritional intervention benefits maternal immunity, egg hatching, and offspring growth. The results of protein and gene quantitative assays showed that the transfer of immune factors into egg whites and yolks depends on maternal levels. Histological observations indicated that the promotion of offspring intestinal development begins in the embryonic period. Microbiota analyses suggested that maternal microbes transfer to the embryonic gut from the magnum to the egg white. Transcriptome analyses revealed that offspring embryonic intestinal transcriptome shifts are related to development and immunity. Moreover, correlation analyses showed that the embryonic gut microbiota is correlated with the intestinal transcriptome and development. Conclusions This study suggests that maternal immunity positively influences offspring intestinal immunity establishment and intestinal development beginning in the embryonic period. Adaptive maternal effects might be accomplished via the transfer of relatively large amounts of maternal immune factors and by shaping of the reproductive system microbiota by strong maternal immunity. Moreover, reproductive system microbes may be useful resources for the promotion of animal health. Video Abstract Graphical Abstract

Beta-alanine is an important amino acid involved in several metabolic reactions in the body. The study aimed to investigate the effect of β-alanine supplementation on intestinal development and the immune performance of weaned piglets. Thirty-two 21-day-old healthy weaned piglets (half female and half male; Duroc × Landrace × Yorkshire) with an initial body weight of 8.11 ± 0.21 kg were randomly divided into 4 groups with 8 replicates of 1 pig each. The control group was fed a basal diet and the three experimental treatment groups were fed diets supplemented with 300, 600 and 1,200 mg/kg β-alanine, respectively. The trial lasted 28 days and the diets fed were divided into 2 phases: the late lactation period (day 1 to 14) and the nursery period (day 15 to 28), during which the weaned piglets had free access to food and water. The regulatory effects of β-alanine were further investigated in vitro using organoids obtained from the jejunum of piglets. In vivo, the addition of β-alanine to the diet had no significant effect on the growth performance of weaned piglets (P > 0.05), but significantly reduced serum levels of immunoglobulin G (IgG) (P

Context. Our previous study revealed that micronised bamboo powder (MBP) can promote the growth performance of broilers aged 1-22 days by improving oxidation resistance, balancing intestinal microflora and regulating metabolic pathways. Aims. This study evaluates the effects of MBP on growth performance, intestinal development, caecal chyme microflora and metabolic pathways of broilers during the grower period, age 24-45 days. Methods. Slow-growing spotted-brown broilers (n = 880, age 24 days) were randomly divided into two groups according to weight and sex and fed either a maize-based diet (control) or a diet with MBP at 1% replacing equivalent maize. Key results. No significant difference was observed in growth performance between MBP and control groups. Broilers receiving MBP exhibited significantly higher (P < 0.05) caecal organ index, jejunum villus height and villi:crypt ratio. The relative abundance of Firmicutes was higher, and relative abundance of Bacteroidetes was lower in the MBP group. Addition of MBP also significantly (P < 0.05) upregulated abundance of p_Firmicutes, f_Alicyclobacillaceae, g_Acutalibacter, f_Peptococcaceae, f_Clostridiaceae, f_Bacillaceae, g_Enterococcus and f_Enterococcaceae, while downregulating abundance of p_Bacteroidetes, f_Bacteroidaceae, g_Bacteroides, o_Bacteroidales and c_Bacteroidia. For the metabolic pathways, 66 were observed to differ between dietary groups, including alanine, aspartic acid and glutamic acid metabolism, butyric acid metabolism, arginine synthesis, linoleic acid metabolism and ^-alanine metabolism. Correlation analysis revealed that Firmicutes in caecal chyme were significantly positively correlated (P < 0.05) with some fatty acids including syringic, 3-methyl-2-oxovaleric, 3-(2-hydroxyphenyl) propanoic and butyric acids. Bacteroidetes were positively correlated (P < 0.05) with some amino acids including L-alanine, L-threonine, 3-methylthiopropionic acid and L-glutamic acid. Conclusions. Replacing maize with MBP at 1% had no negative effect on growth performance of broilers. Feeding MBP improved intestinal development and increased the relative abundance of bacteria that promotes fatty acid metabolism and fibre degradation. Implications. MBP provides an alternative to maize and is a beneficial source of fibre; further research is warranted to determine the optimum feeding level. [ABSTRACT FROM AUTHOR]

Background: Probiotics comprise effective feed additives that can replace antibiotics in animal livestock production. However, mono‐strain probiotics appear less effective because of their instability. Therefore, the present study aimed to investigate dietary supplementation with compound probiotics (CPP) on growth performance, diarrhea rate and intestinal mucosal barrier, as well as the possible molecular mechanism, in chicks. In total, 360 1‐day‐old chicks of the Hy‐Line Brown Chicks were randomly divided into the control group (CON, basal diet), chlortetracycline group (500 mg kg−1 CTC) and compound probiotics group (1000 mg kg−1 CPP, consisting of Bacillus subtilis, Bacillus licheniformis, Enterococcus faecium and yeast). The experiment period was 56 days. Results: The results showed that, in comparison with the CON group, CPP significantly increased the average daily feed intake and average daily gain of chicks and reduced diarrhea (P < 0.05). The probiotic group exhibited increased immune organ (i.e. spleen and thymus) mass and increased levels of serum immunoglobulin (Ig)A, IgM and IgG (P < 0.05) compared to the CTC group. In addition, the jejunal mass and morphology were improved in the probiotic group (P < 0.05). Moreover, CPP reinforced jejunal barrier function, as indicated by increased transepithelial electrical resistance, protein expression of occludin and claudin‐1, and diamine oxidase levels in the jejunum (P < 0.05). Likewise, enhanced fluorescence signals of proliferating cell nuclear antigen‐labeled mitotic cells and villin‐labeled absorptive cells in the jejunum (P < 0.05) suggested that CPP promoted intestinal stem cells activity. Mechanistically, the Wnt/β‐catenin signaling pathway, including β‐catenin, TCF4, c‐Myc, cyclin D1 and Lgr5, was amplified in the jejunum by CPP addition (P < 0.05). Conclusion: The present study demonstrated that dietary supplementation with CPP reinforced the jejunal epithelial integrity by activating Wnt/β‐catenin signaling and enhanced immune function in chicks. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

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Osteopontin (OPN) is a multifunctional protein found in all vertebrates. OPN is expressed in many different cell types, and is consequently found in most tissues and physiological secretions. OPN is involved in a multitude of biological processes, such as activation and regulation of the immune system; biomineralization; tissue-transformative processes, including growth and development of the gut and brain; interaction with bacteria; and many more. OPN is found in the highest concentrations in milk, where it is believed to initiate and regulate developmental, immunological and physiological processes in infants who consume milk. Processes for the isolation of bovine OPN for use in infant formula have been developed, and in recent years, many studies have investigated the effects of the intake of milk OPN. The purpose of this article is to review and compare existing knowledge about the structure and function of milk OPN, with a particular focus on the effects of milk OPN on human health and disease. [ABSTRACT FROM AUTHOR]

The development of the mammalian intestine, from its earliest origins as a morphologically uniform sheet of endoderm cells during gastrulation into the complex organ system that is essential for the life of the organism, is a truly fascinating process. During midgestation development, reciprocal interactions between endoderm-derived epithelium and mesoderm-derived mesenchyme enable villification, or the conversion of a radially symmetric pseudostratified epithelium into the functional subdivision of crypts and villi. Once a mature crypt–villus axis is established, proliferation and differentiation of new epithelial cells continue throughout life. Spatially localized signals including the wingless and Int-1, fibroblast growth factor, and Hippo systems, among others, ensure that new cells are being born continuously in the crypt. As cells exit the crypt compartment, a gradient of bone morphogenetic protein signaling limits proliferation to allow for the specification of multiple mature cell types. The first major differentiation decision is dependent on Notch signaling, which specifies epithelial cells into absorptive and secretory lineages. The secretory lineage is subdivided further into Paneth, goblet, tuft, and enteroendocrine cells via a complex network of transcription factors. Although some of the signaling molecules are produced by epithelial cells, critical components are derived from specialized crypt-adjacent mesenchymal cells termed telocytes, which are marked by Forkhead box l1, GLI Family Zinc Finger 1, and platelet-derived growth factor receptor α. The crucial nature of these processes is evidenced by the multitude of intestinal disorders such as colorectal cancer, short-bowel syndrome, and inflammatory bowel disease, which all reflect perturbations of the development and/or differentiation of the intestine.

In this study, five strains of Lactobacillus rhamnosus (L. rhamnosus) and two strains of Lactobacillus paracasei (L. paracasei) isolated from infants were used as experimental bacteria and Lactobacillus rhamnosus GG as control strain. The gastrointestinal tolerance of these strains were evaluated through acid resistance test, bile salt resistance test, hydrophobicity test, automatic aggregation ability test and cell adhesion test. L. rhamnosus SW-02, the best effective strain in tolerance evaluation, was co-cultured with intestinal organoids. The morphology and growth status of organoids were observed by inverted phase contrast microscope, and the budding rate was calculated. The proliferation status of organoids was evaluated by EdU staining. The mRNA levels of the proliferation marker Ki67, intestinal stem cell marker Lgr5 and tight junction protein Zo-1 were measured by real-time PCR. The level of MUC2 was detected by enzyme-linked immunosorbent assay. The results showed that compared with other strains, L. rhamnosus SW-02 had strong acid resistance. SW-02, SW-03 and SW-X had strong bile salt resistance. LGG, SW-01, SW-02, SW-04 and TX-02 had high hydrophobicity to xylene. For automatic aggregation capability, SW-01 was the strongest, followed by SW-02. The adhesion test of HT-29 cells showed that SW-01, SW-02 and SW-03 had high adhesion ability. When SW-02 was co-cultured with intestinal organoids, SW-02 significantly (P

Abstract Background Research on nutrition in early-life commonly focuses on the maturation of the intestine because the intestinal system is crucial for ensuring continued growth. To explore the importance of early nutrition regulation in animals, soy protein concentrate (SPC) was added to the early diet of broilers to investigate its effects on amino acid digestibility, intestinal development, especially intestinal microorganisms, and broiler metabolites. A total of 192 one-day-old Arbor Acres (AA) male broilers were randomly assigned to two experimental treatments with 8 replicates of 12 birds. The control group was fed a basal diet (control), and the treatment group was fed a basal diet supplemented with 12% SPC (SPC12) during the first 10 d (starter phase). From d 11 to 21 (grower phase) and d 22 to 42 (finisher phase), a basal diet was fed to both treatment groups. Results SPC reduced the pH value and acid-binding capacity of the starter diet (P

Microbes commonly administered to chickens facilitate development of a beneficial microbiome that improves gut function, feed conversion and reduces pathogen colonization. Competitive exclusion products, derived from the cecal contents of hens and shown to reduce Salmonella colonization in chicks, possess important pioneer-colonizing bacteria needed for proper intestinal development and animal growth. We hypothesized that inoculation of these pioneer-colonizing bacteria to day of hatch chicks would enhance the development of their intestinal anatomy and microbiome. A competitive exclusion product was administered to broiler chickens, in their drinking water, at day of hatch, and its impact on intestinal morphometrics, intestinal microbiome, and production parameters, was assessed relative to a control, no treatment group. 16S rRNA gene, terminal restriction fragment length polymorphism (T-RFLP) was used to assess ileal community composition. The competitive exclusion product, administered on day of hatch, increased villus height, villus height/width ratio and goblet cell production ∼1.25-fold and expression of enterocyte sugar transporters 1.25 to 1.5-fold in chickens at 3 days of age, compared to the control group. As a next step, chicks were inoculated with a defined formulation, containing Bacteroidia and Clostridia representing pioneer-colonizing bacteria of the two major bacterial phyla present in the competitive exclusion product. The defined formulation, containing both groups of bacteria, were shown, dependent on age, to improve villus height (jejunum: 1.14 to 1.46-fold; ileum: 1.17-fold), goblet cell numbers (ileum 1.32 to 2.51-fold), and feed efficiency (1.18-fold, day 1) while decreasing Lactobacillus ileal abundance by one-third to half in birds at 16 and 42 days of age, respectively; compared to the phosphate buffered saline treatment group. Therefore, specific probiotic formulations containing pioneer colonizing species can provide benefits in intestinal development, feed efficiency and body weight gain. [ABSTRACT FROM AUTHOR]

Background: Nutrition drives immunity and health in animals, and maternal immunity benefits offspring. In our previous study, a nutritional intervention strategy was found to promote the immunity of hens, which subsequently improved immunity and growth in offspring chicks. Maternal effects clearly exist, but how are mothers' immune advantages transferred to their offspring, and how do they benefit them? Results: Here, we traced the beneficial effects back to the process of egg formation in the reproductive system, and we focused on the embryonic intestinal transcriptome and development, as well as on maternal microbial transfer in offspring. We found that maternal nutritional intervention benefits maternal immunity, egg hatching, and offspring growth. The results of protein and gene quantitative assays showed that the transfer of immune factors into egg whites and yolks depends on maternal levels. Histological observations indicated that the promotion of offspring intestinal development begins in the embryonic period. Microbiota analyses suggested that maternal microbes transfer to the embryonic gut from the magnum to the egg white. Transcriptome analyses revealed that offspring embryonic intestinal transcriptome shifts are related to development and immunity. Moreover, correlation analyses showed that the embryonic gut microbiota is correlated with the intestinal transcriptome and development. Conclusions: This study suggests that maternal immunity positively influences offspring intestinal immunity establishment and intestinal development beginning in the embryonic period. Adaptive maternal effects might be accomplished via the transfer of relatively large amounts of maternal immune factors and by shaping of the reproductive system microbiota by strong maternal immunity. Moreover, reproductive system microbes may be useful resources for the promotion of animal health. 53Hwa7T4Jvj9omDXNYBHSH Video Abstract [ABSTRACT FROM AUTHOR]

Effects of different feeding strategies on meat quality and intestinal development in pigeon squabs were investigated. 120 pairs of pigeons with two squabs each were assigned to five groups (four free-choice feeding systems and one complete feeding system): T1 (corn, pea, wheat, and pelleted feed), T2 (corn, wheat, and pelleted feed), T3 (corn, pea, and pelleted feed), T4 (corn and pelleted feed), and T5 (complete pelleted feed). Compared with T5, the diet in T4 made the breast meat redder and more yellow (p < 0.05). T2 and T4 resulted in an enhanced total superoxide dismutase activity of meat. Breast muscle in T1 and T2 was determined to have higher contents of nonessential amino acids, glycine, alanine, and glutamic acid (p < 0.05). The contents of the essential amino acids, lysine, threonine, valine, histidine, and arginine were also higher in T1 (p < 0.05). Villus height, surface area, and alkaline phosphatase activity of the duodenum and jejunum in T2 were the highest among the treatments (p < 0.05). In conclusion, free-choice feeding system can improve the meat quality and intestinal development of pigeon squabs, but which combination method of whole grains to use in the production depends on the feeding purpose. [ABSTRACT FROM AUTHOR]

Little information is available on age- and creep-feeding-related microbial and immune development in neonatal piglets. Therefore, we explored age- and gut-site-specific alterations in the microbiome, metabolites, histo-morphology, and expression of genes for microbial signaling, as well as immune and barrier function in suckling and newly weaned piglets that were receiving sow milk only or were additionally offered creep feed from day of life (DoL) 10. The experiment was conducted in two replicate batches. Creep feed intake was estimated at the litter level. Piglets were weaned on day 28 of life. Gastric and cecal digesta and jejunal and cecal tissue were collected on DoL 7, 14, 21, 28, 31, and 35 for microbial and metabolite composition, histomorphology, and gene expression. In total, results for 10 piglets (n = 5/sex) per dietary group (sow milk only versus additional creep feed) were obtained for each DoL. The creep feed intake was low at the beginning and only increased in the fourth week of life. Piglets that were fed creep feed had less lactate and acetate in gastric digesta on DoL 28 compared to piglets fed sow milk only (p

ABSTRACT: This research aimed to investigate the effects of drinking water temperatures on growth performance, water consumption, surface temperature, organ indices, blood parameters, and intestinal development of geese, and determine the optimal drinking water temperature for 21 to 49-d geese. A total of 192 twenty-one-day male Yuzhou white geese were allocated randomly to 4 groups with 8 replicate pens per group according to the drinking water temperature (drinking water temperature [7℃–12℃] at ambient temperature [TC], 18℃ [T1], 27℃ [T2], and 36℃ [T3], respectively). The results showed that increasing drinking water temperature did not significantly improve the BW, ADG, and ADFI of geese (P > 0.05), whereas drinking warm water of 36℃ for geese had a trend to increase FCR (0.05 < P < 0.1). Geese in group T2 drank the most water per day on average, whereas geese in group TC drank the least (P < 0.001). Geese in groups T1, T2, and T3 had higher eyes temperatures than group TC (P < 0.001). No significant differences were found on the organ indices and blood biochemical parameters (P > 0.05). Geese from group T1 had higher crypt depth and muscularis thickness of duodenum (P < 0.05), and lower ratio of villus height to crypt depth than other groups (P < 0.001). Geese from group T1 had higher activities of trypsin in duodenum and jejuna and amylase in jejuna at 49 d than other groups (P < 0.01). Overall, these data indicate drinking water at 18℃ can increase water consumption and eyes temperature, and improve the activity of digestive enzymes and promote intestinal development. Under our experimental conditions, we recommend that the optimal drinking water temperature for geese from 21 to 49 d of age is 18°C.