1. Melatonin inhibits cell proliferation in a rat model of breast hyperplasia by mediating the PTEN/AKT pathway
- Author
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Yibo Huang, Yingqun Niu, and Xiangjian Zhang
- Subjects
0301 basic medicine ,Cancer Research ,Breast Hyperplasia ,melatonin ,Melatonin ,Random Allocation ,03 medical and health sciences ,Mammary Glands, Animal ,0302 clinical medicine ,hyperplastic disease of the breast ,Proliferating Cell Nuclear Antigen ,medicine ,Animals ,Tensin ,PTEN ,Rats, Wistar ,skin and connective tissue diseases ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,breast hyperplasia ,Hyperplasia ,biology ,Chemistry ,PTEN/AKT pathway ,PTEN Phosphohydrolase ,General Medicine ,Articles ,mammary gland hyperplasia ,Immunohistochemistry ,Rats ,Proliferating cell nuclear antigen ,Disease Models, Animal ,030104 developmental biology ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Female ,Proto-Oncogene Proteins c-akt ,Signal Transduction ,medicine.drug - Abstract
In the present study, a rat model of breast hyperplasia was established via the administration of estradiol benzoate and progesterone. Subsequent changes associated with breast hyperplasia were then investigated by measuring the diameter and height of the nipples and by staining breast tissue with hematoxylin and eosin. The proliferation and apoptosis of hyperplastic cells in the breast tissue were then determined by analyzing the expression of proliferating cell nuclear antigen (PCNA) and cleaved-caspase-3 by immunohistochemistry and TUNEL staining. We also determined the expression of proteins associated with the phosphatase and tensin homolog (PTEN)/protein kinase B (AKT) signaling pathway by western blotting. Melatonin treatment led to a significant reduction in the degree of breast hyperplasia (P
- Published
- 2021