1. Treatment Paradigms for Retinal and Macular Diseases Using 3-D Retina Cultures Derived From Human Reporter Pluripotent Stem Cell Lines
- Author
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Wei Zheng, Jutaro Nakamura, Anand Swaroop, Vijender Chaitankar, Sam Michael, Kohei Homma, Tiziana Cogliati, Koray Dogan Kaya, Gregory J. Tawa, Rossukon Kaewkhaw, Jizhong Zou, Mahendra Rao, Matthew Brooks, and Manju Swaroop
- Subjects
Pluripotent Stem Cells ,0301 basic medicine ,Retinal degeneration ,Cellular differentiation ,Retinal Pigment Epithelium ,Biology ,human retina development ,Retina ,drug discovery ,Cell Line ,Tissue Culture Techniques ,Macular Degeneration ,03 medical and health sciences ,chemistry.chemical_compound ,Genes, Reporter ,disease modeling ,medicine ,Animals ,Humans ,Induced pluripotent stem cell ,next generation sequencing ,Retinal pigment epithelium ,Special Issue ,Gene Expression Profiling ,Retinal Degeneration ,chemical screens ,three-dimensional organoid culture ,Cell Differentiation ,Retinal ,Anatomy ,Macular degeneration ,medicine.disease ,photoreceptor ,3. Good health ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,fluorescent reporter ,Stem cell ,transcriptome ,Neuroscience ,high throughput screening ,Retinal Neurons - Abstract
We discuss the use of pluripotent stem cell lines carrying fluorescent reporters driven by retinal promoters to derive three-dimensional (3-D) retina in culture and how this system can be exploited for elucidating human retinal biology, creating disease models in a dish, and designing targeted drug screens for retinal and macular degeneration. Furthermore, we realize that stem cell investigations are labor-intensive and require extensive resources. To expedite scientific discovery by sharing of resources and to avoid duplication of efforts, we propose the formation of a Retinal Stem Cell Consortium. In the field of vision, such collaborative approaches have been enormously successful in elucidating genetic susceptibility associated with age-related macular degeneration.
- Published
- 2016