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Copyright of Florence (1801-464X) is the property of Care Comm s.r.o. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Biogenic amines (BAs) are Nitrogen-containing organic compounds that are important in aquaculture and food. BAs are formed by decarboxylation through various chemical reactions with bacterial activities in protein-rich fish and other foods. Biogenic amines are more common in sea fish such as tuna, sardines and mackerel. The presence of biogenic amines has also been found in foods such as spoiled fruits and vegetables. Bacteria-induced histamine poisoning in fish causes allergies as well as various disorders such as headache, flushing, abdominal cramps and hypertension. Many cases of fish poisoning occur due to histamines formed in fish such as Mackerel (Scrombridae) consumed in daily life. Biogenic amines, which are objectionable in terms of food safety, are also formed in cheese, dairy products, fruits and vegetables and other foods rich in protein along with fish. In general, the level of 1000 mg/kg and above in foods is considered a critical and dangerous level for public health. [ABSTRACT FROM AUTHOR]

Copyright of Indonesian Fisheries Processing Journal / Jurnal Pengolahan Hasil Perikanan Indonesia is the property of IPB University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Istanbul Gelisim University Journal of Health Sciences / İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi is the property of Istanbul Gelisim Universitesi Saglik Bilimleri Yuksekokulu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Medicina Pro Praxi is the property of SOLEN sro and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of MESO is the property of Zadruzna Stampa D.D. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Ikan tuna termasuk komoditi yang mudah rusak sehingga perlu diolah untuk memperpanjang umur simpan, salah satu caranya dengan pengalengan. Penelitian tentang pengalengan tuna dari perairan Aceh belum pernah dilakukan. Oleh karena itu, penelitian ini bertujuan untuk mempelajari kualitas tuna kaleng yang disterilisasi menggunakan alat pressure canner berkapasitas 24L dengan memvariasikan suhu dan lama sterilisasi (suhu 121°C selama 20 menit dan suhu 115°C selama 50 menit) serta jenis medium (larutan garam dan minyak kelapa sawit). Ikan tuna yang dikalengkan diperoleh dari perairan Aceh. Parameter kualitas bahan baku yang diuji pada tuna segar adalah kadar histamin, angka lempeng total (ALT) dan pH. Sementara itu, parameter kualitas yang diuji pada tuna kaleng adalah ALT, pH, kandungan logam berat (timbal dan merkuri) serta tingkat penerimaan konsumen melalui uji organoleptik (hedonik). Hasil penelitian menunjukkan bahwa ALT tuna kaleng pada semua perlakuan

In this study, biogenic amine contents of 17 different fish products (frozen Alaska Pollock, anchovy fillets, sardine fillets, hake fillets, pangasius fillets, Atlantic bonito, and Atlantic salmon; smoked mackerel, trout, Atlantic salmon; marinated mackerel, anchovy, anchovy with red pepper sauce, anchovy with hot pepper sauce; smoked--marinated mackerel and anchovy) available on Turkish retail market were investigated using rapid HPLC method. Putrescine, spermidine, spermine, and dopamine were the most abundant biogenic amines found in fish products. Among fish products tested, histamine accumulation was only found for frozen Atlantic salmon, marinated dried mackerel, and marinated anchovy with hot pepper sauce, and remained below the legal limit suggested as 10 mg/100 g. Tyramine accumulation in fish products was higher than histamine. The highest tyramine content was observed from frozen Atlantic salmon, marinated anchovy with hot pepper sauce, and smoked-marinated mackerel, with a corresponding value of 8.5, 5.27, and 3.5 mg/100 g. Putrescine and cadaverine level of fish products were found as <33.53 and <19.87 mg/100g, respectively. Trimethylamine (TMA) contents of fish products were between 6.6 and 22 mg/100 g. The lowest total biogenic amine content was detected in frozen Alaska pollock (28.8 mg/100 g), whilst frozen Atlantic salmon had the highest level of total biogenic amine (232.3 mg/100 g). The study results showed that most of the fish products tested were of good quality. However, these results revealed that careful monitoring studies should be made to ensure the safety of fishery products in human consumption. [ABSTRACT FROM AUTHOR]

Copyright of Medicina Pro Praxi is the property of SOLEN sro and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Since decades, histamin applications are routinely performed in skin prick tests using a lancet. However, this technique is associated with various drawbacks. Materials and Methods: In healthy human subjects, we investigated the effects of microneedle‐enhanced histamin delivery (wheal size, erythema size) in the skin microvasculature using polarized light spectroscopy imaging (Tissue Viability imaging, TiVi). Histamin was applied on microneedle‐pretreated skin or on ‐untreated skin, to assess the microvascular response in the local skin. Results: In our results, histamin was delivered more rapidly into the skin after microneedle pretreatment compared to passive diffusion, visible as wheal and erythema. Conclusion: The here presented technique might be useful for a personalized drug‐testing system in the future. [ABSTRACT FROM AUTHOR]

İki mediatör, histamin ve bradikinin, anjioödem vakalarının çoğundan sorumludur. Anjioödem ürtikerin bir belirtisi olarak kabarıklıkla beraber olabilir ve bu şekli çoğunlukla histaminerjik (allerjik, IgE-aracılı) tiple ilişkilidir. Histaminerjik anjioödem en sık anjioödem nedenidir. Anjioödem, akut (semptomlar 6 haftadan kısa sürerse) veya kronik (semptomlar 6 haftadan uzun sürerse) olarak da ayrılabilir. Anjioödem, ürtikerle (kaşıntılı kabarıklık) beraber olup olmamasına göre de daha ileri sınıflanabilir. Kaşıntılı kabarıkla beraber olan anjioödem, akut ya da kronik, spontan ya da indüklenebilir bir ürtikerin göstergesidir. Doğru teşhis ve uygun tedaviyi seçmek için, klinik tablonun bariz heterojenliğinden dolayı, anjioödem fenotipinin ve semptomların altında yatan medyatörün iyi bilinmesi esastır. Bu derlemede, kabarıkla beraber olan/olmayan histaminerjik anjioödeme özgün epidemiyoloji, patofizyoloji ve klinik spektrum ve ayırıcı tanı detaylı olarak tartışılmaktadır.

Biyojen aminler daha çok proteince zengin ve fermente gıdalarda mikroorganizma faaliyetleri sonucu oluşan ve sağlık üzerinde önemli etkileri olan bileşikler olmakla birlikte yüksek konsantrasyonlarda toksik etki gösteren bileşiklerdir. Bu nedenle mikroorganizma gelişimlerini etkileyen faktörler, biyojen amin oluşumunu da etkilemektedir. Biyojen aminlerin oluşumunu etkileyen temel faktörler; şeker konsantrasyonu, pH, sıcaklık, tuz konsantrasyonu, gıdadaki mikroorganizma türleri, starter kültürler, oksijen ve benzeri faktörler olarak gösterilmektedir. Biyojen aminler nitrit ve azot oksitler ile reaksiyona girebilmekte ve kanserojen nitrozaminleri oluşturabildikleri gibi, bir biyojen amin formundan diğer bir biyojen amin formuna da dönüşebilmektedirler. Ayrıca bazı poliaminlerin, poliamin oksidaz enzimiyle katabolizması sonucu toksik bir bileşik olan akrolein de oluşabilmektedir. Bu derlemede; biyojen aminlerin oluşum mekanizmaları, etkili faktörler ve diğer bileşiklere dönüşümleri literatür verileri incelenerek ele alınmıştır.

Biyojen aminler daha çok proteince zengin ve fermente gıdalarda mikroorganizma faaliyetleri sonucu oluşan ve sağlık üzerinde önemli etkileri olan bileşikler olmakla birlikte yüksek konsantrasyonlarda toksik etki gösteren bileşiklerdir. Bu nedenle mikroorganizma gelişimlerini etkileyen faktörler, biyojen amin oluşumunu da etkilemektedir. Biyojen aminlerin oluşumunu etkileyen temel faktörler; şeker konsantrasyonu, pH, sıcaklık, tuz konsantrasyonu, gıdadaki mikroorganizma türleri, starter kültürler, oksijen ve benzeri faktörler olarak gösterilmektedir. Biyojen aminler nitrit ve azot oksitler ile reaksiyona girebilmekte ve kanserojen nitrozaminleri oluşturabildikleri gibi, bir biyojen amin formundan diğer bir biyojen amin formuna da dönüşebilmektedirler. Ayrıca bazı poliaminlerin, poliamin oksidaz enzimiyle katabolizması sonucu toksik bir bileşik olan akrolein de oluşabilmektedir. Bu derlemede; biyojen aminlerin oluşum mekanizmaları, etkili faktörler ve diğer bileşiklere dönüşümleri literatür verileri incelenerek ele alınmıştır.

Copyright of Galicia Clínica is the property of Sociedad Gallega de Medicina Interna and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The impacts of water and ethanolic extracts of propolis on growth of Gram- positive and Gram-negative food-borne pathogens and their biogenic amine production were investigated. Ethanolic extracts of propolis had lower minimum inhibitory concentration (MIC) than that of water extract. Undiluted water extract of propolis (100%) was more effective on growth inhibition of Staphylococcus aureus (29.5 mm) and Klebsiella pneumoniae (26.5 mm) than antibiotics. The effect of propolis on biogenic amine production showed a discrepancy depending on bacterial strains, specific amine and extract type. Water or ethanolic extracts of propolis induced about 81-fold lower histamine accumulation by Yersinia enterocolitica. The study results suggested that both propolis extracts tested could be used as antimicrobial as they inhibit biogenic amines which were toxically important, although both propolis extracts exerted variability even among Gram- positive or negative bacteria. Moreover, stimulation of serotonin production by bacteria in the presence of propolis extracts emphasized important aspects of propolis for utilization in foods. [ABSTRACT FROM AUTHOR]

The purpose of the current study was to investigate the biogenic amine production of lactic acid bacteria (LAB) strains (Lactobacillus pentosus, Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, Lactobacillus fermentum, Lactococcus raffinolactis, Pediococcus pentosaceus and Leuconostoc mesenteroides) which in fermented foodstuff. Biogenic amine production of LAB strains was monitored in histidine decarboxylase broth using HPLC method. Significant differences were observed on ammonia (AMN) and biogenic amines accumulation among LAB strains (p<0.05). Although P. acidilactici produced lesser AMN than other LAB, it was observed that all LAB strains produced high amount of AMN. LAB strains produced all amines in amounts between 1.48 and 1187.50 mg/L. Histamine production by LAB was in range from 17.20 mg/L by P. acidilactici to 126.26 mg/L by Lb. fermentum. The lowest tyramine concentration was observed by P. acidilactici with value of 14.66 mg/L whilst the highest tyramine production was found by L. raffinolactis with value of 64.43 mg/L. [ABSTRACT FROM AUTHOR]

Background and Design: Urticaria is an intensely pruritic dermatologic disorder characterized by temporary erythematous and edematous lesions. Immunoglobulin E (IgE), mast cells, and histamine play a major role in its pathogenesis. H1 antihistamines are the basis of the treatment, but they may not be sufficient for some cases. The latest guidelines advise using omalizumab, anti-IgE monoclonal antibody, for patients with refractory urticaria. Our aim was to evaluate the efficacy and safety of omalizumab in patients with chronic spontaneous urticaria and share the real-life experiences in our patients. Materials and Methods: This study, which covers the 50 months between April 2014 and June 2018, includes data of 124 chronic spontaneous urticaria patients treated with monthly subcutaneous injections of omalizumab. The 7-day urticaria activity score (UAS7) was used to compare the disease severity before and after the treatment. Results: A total of 124 patients, consisting of 75 females (60.4%) and 49 males (39.6%), were enrolled. The mean UAS7 scores before and after the treatment were 32.4 and 2.8, respectively. Before the treatment, 107 of 124 patients (86.2%) had severe urticaria, while the remaining 17 patients (13.8%) had a moderate disease. Urticarial lesions of 78 patients (63%) completely disappeared under treatment. Thirtyone patients (25%) had the disease under control. Twelve patients (9.6%) still had mild disease, and the remaining three patients (2.4%) had moderate refractory symptoms. There was no correlation between treatment response rates and the presence of thyroid autoantibodies or high IgE levels. There was no severe side effect observed during the treatment of up to 36 months (mean 11 months). Conclusion: Our results supported the literature showing that omalizumab is a rapid-acting, effective, and safe treatment choice for chronic spontaneous urticaria. [ABSTRACT FROM AUTHOR]

Pterjium is an ocular surface disorder and is a pathologic result of corneal invasion of the fibro-vascular proliferation of the bulb conjunctiva in the region corresponding to the lid space. Pterjium is a disease with high rates of recurrence after surgery. Nowadays, along with several new techniques that are used to prevent recurrence, antineoplastics are also used to stop the growth. Various growth factors and mediators, like some neoplasms, have been found to be effective in pterygium growth, invasion and relapse. In addition to growth factors such as stem cell factor and VEGF, an increase in histamine, carbonic anhydrase activity, in number of eph B4, and in nestin release was found while a decrease in IGFBP3 release was found. Studies are currently underway to stop the growth of pterygium and prevent recurrence.

Bu çalışmada, marine edildikten sonra farklı şekillerde paketlenip 4±1°C’de muhafaza edilen tirsi (Alosa immaculata, Bennett, 1838) filetolarında biyojenik aminlerin ve mikrobiyolojik değişimlerin belirlenmesi amaçlanmıştır. Marine örneklerde mikrobiyolojik kriter olarak toplam aerob mezofilik bakteri, toplam psikrofil bakteri, maya-küf, koliformve toplam histamin üreten bakteri sayıları ve türleri belirlenmiştir. Ayrıca bakterilerin moleküler ve biyokimyasal identifikasyonu gerçekleştirilmiştir. Depolama sonunda salamura, yağda ve vakum paketlenen marine tirside histamin miktarı sırasıyla 2.19, 2.09 and 2.41 ppm (mg kg-1), TAMB sayıları; 2.49, 2.75 ve 2.68 log kob g-1 olarak tespit edilmiştir. Histamin üreten bakterilerin sayıları 7 aylık depolama boyunca saptanabilir düzeyin altında kalmıştır. Histamin üreten bakterilerden Pantoea agglomerans, Shewanella baltica, Brevundimonas diminuta, Pseudomonas putida, Acinetobacter calcoaceticus tanımlanmıştır, salamura ve yağda paketlenen marine tirsinin biyojenik aminlerden histamin ve mikrobiyolojik kriterler açısından 7 ay, vakum paketlenen marine tirsinin ise 6 ay 4±1°C’de depolanabileceği sonucuna varılmıştır.

Copyright of HNO is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Résumé La mastocytose est une affection caractérisée par une accumulation tissulaire anormale de mastocytes, le plus souvent dans la peau. Les complications osseuses de la mastocytose sont très polymorphes allant de l'ostéoporose à des tableaux d'ostéosclérose. Les complications osseuses sont liées à l'infiltration par les mastocytes et à la libération de médiateurs par les mastocytes stimulés. Compte tenu du risque osseux, il est utile de faire une mesure de la densité minérale osseuse (DMO) et des radiographies rachidiennes à la recherche de fractures vertébrales. L'atteinte osseuse peut également révéler le diagnostic et il faut savoir y penser en cas d'ostéopathie fragilisante fracturaire. Il existe peu de données montrant le bénéfice osseux des traitements de la mastocytose. Il est logique de proposer un traitement anti-ostéoporotique de type antirésorbeur compte tenu de l'augmentation de la résorption osseuse, chez les patients à risque élevé de fracture. Abstract Mastocytosis are characterized by an abnormal tissular proliferation of mast cells in at least one visceral organ. Considering the accumulation of mast cells in the bone marrow, bone complications as osteoporosis with an increased risk of vertebral fractures can be observed. Bone involvement may also be the sole presentation of abnormal proliferation and the diagnosis of mastocytosis should be discussed in case of bone fragility in young patients. Bone complications are related to mast cell's infiltration and to mediators' release by stimulated mast cells. Considering bone involvement, it is useful to assess bone mineral density (BMD) and to perform bone radiographs, for the assessment of vertebral fractures. There are very few data on the bone efficacy of mastocytosis treatments. Antiresorptive treatments as bisphosphonates are logical because of the key role of osteoclast and are indicated in patients with history of low trauma fracture and/or low BMD. [ABSTRACT FROM AUTHOR]

Copyright of Der Hautarzt is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

An increase in seafood-related illnesses as a consequence of an increase in seafood consumption has been documented recently. The United States Food and Drug Administration (FDA) has identified histamine as the major chemical hazard of seafood. The aim of this study was to determine histamine levels in 227 production lots (2043 subsamples) of canned tuna, canned sardines, canned mackerel and smoked salmon obtained from Serbian retail stores in 2018. Levels of histamine were determined using high performance liquid chromatography tandem mass-spectrometry. In addition, we compared histamine levels found in analysed products with levels established by the European Commission (EC) and FDA. Mean levels of histamine in compliant lots, according to EC Regulation 2073/2005, were 9.21, 3.16, 3.34 and 5.22 mg/kg in canned tuna, canned sardines, canned mackerel and smoked salmon, respectively. The highest histamine levels were found in canned tuna and canned mackerel, 1112 mg/kg and 412 mg/kg, respectively. Histamine was detected in 468 (22.91%) of all analysed subsamples. Levels above 100 mg/kg, which is the maximum histamine level allowed in canned fi sh in the EU, were found in 38 (1.86%) of analysed subsamples. According to EC Regulation 2073/2005, six (2.64%) of all analysed lots were found non-compliant, while 21 (9.25%) of analysed lots should be rejected according to the FDA limit (50 mg/kg) [ABSTRACT FROM AUTHOR]

Copyright of Der Hautarzt is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Biogenic amines mainly occur by microbial activity in high-protein foods and fermented foods and have important health effects, but may cause toxicological effects at high concentrations. For this reason, the factors influencing microbial growth also affects production of biogenic amines. The factors that affect the formation of biogenic amines are carbohydrate concentration, pH, temperature, sodium chloride concentration, types of microorganisms in the foods, starter cultures, oxygen and so on. While biogenic amines can react with nitrosating reagents such as nitrite and nitrogen oxides to form carcinogenic nitrosamines, they are also able to transform from one biogenic amine form to another. In addition, some polyamines could produce toxic acrolein by their catabolism with polyamine oxidase. The aim of this paper is to give information about the occurrence and formation mechanisms of biogenic amines, factors influencing their formation, and transformation of biogenic amines to other compounds. [ABSTRACT FROM AUTHOR]

Copyright of MESO is the property of Zadruzna Stampa D.D. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Firat Universitesi Saglik Bilimleri Veteriner Dergisi is the property of Firat Universitesiu, Saglik Bilimleri Enstitusu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background/Aim. Bronchial asthma is a disease that is characterized by the variability of the clinical picture, physical and functional status and the existence of bronchial hypersensitivity and hyperreactivity with varying degrees. Bronchial responsiveness and sensitivity are tested in patients with clinically suspected existence of asthma and normal spirometry test. The aim of the study was to analyze the patients with atopic asthma and study test results of skin sensitization to inhaled allergens, nonspecific bronchial hyperreactivity and specific hyperreactivity estimated by bronchial provocation tests with inhalant allergens. Methods. The prospective study at the Pulmonology Clinic of the Military Medical Academy in Belgrade Serbia, during 2014, included 70 male subjects aged 18-30 years, who had perennial asthma symptoms. All subjects were nonsmokers, with normal spirometry findings, with normal radiological chest findings and with no symptoms of respiratory infection over the past two months. All respondents were tested with skin prick tests with inhalant allergens and nonspecific bronchial provocation test with histamine. On the basis of histamine test, subjects were divided into two groups: the group I, in which there was a slight degree of hypersensitivity [provocation concentration of histamine causing a 20% fall in forced expiratory volume -PC20 = 6.09 ± 1.1 mg/mL] and the group II with negative histamine test (PC20 = 14.58 ± 6.34 mg/mL). Specific bronchial provocation test was performed in all patients, and the selection of the allergens was carried out based on the results of testing of skin hypersensitivity. Results. Results of skin sensitization show the highest incidence of mites Dermatophagoides pterronissinus (83.3% group I and 85.0% group II) followed by grass pollen (53.3% group I and 52.0% group II) and house dust (33.3% group I and 50.0% group II). There were no statistically significant differences in allergens between groups (p > 0.05). In both groups, spirometry findings were within normal values [forced vital capacity - FVC and forced expiratory volume 1 - FEV1 > 80% predictive value], but statistically significant difference was found in FEV1 between groups (p < 0.05). Specific bronchial provocation tests with solutions of inhaled allergens in both groups caused a significant decline in FEV1 (⩾ 20%) in all patients individually. No statistically significant differences were found neither between groups, nor between individual allergens (average decline in FEV1: Group I 32.9 ± 2.4% and group II 31.5 ± 2.2%). Conclusion. There is no relationship between the degree of specific and non-specific bronchial hyperreactivity in patients with allergic asthma. [ABSTRACT FROM AUTHOR]

In this study, it was aimed to determine the biogenic amines and microbiological changes of shad (Alosa immaculata, Bennett, 1838) fillets, which were marinated, differently packaged and stored at 4±1 °C. Total aerobic count, psychrophilic flora count, mould-yeast, coliform and histamine forming bacteria count as microbiological criteria of marinated fish were investigated. In addition to that biochemical and molecular identification of isolated bacteria were performed. At the end of storage period, for marinated shad of different packed with brine, oil and vacuum packed were determined histamine values as 2.19, 2.09 and 2.41 mg kg-1, and TAC values as 2.49, 2.75 and 2.68 log kob g-1, respectively. Pantoea agglomerans, Shewanella baltica, Brevundimonas diminuta, Pseudomonas putida and Acinetobacter calcoaceticus were identified as histamine producing bacteria. For histamine and microbiological criteria; It was determined that brine and oil recipe shad marinade can be stored at 4±1 °C for 7 months, while vacuum packed ones can be stored in the same conditions for 6 months. [ABSTRACT FROM AUTHOR]

Copyright of Firat Universitesi Saglik Bilimleri Veteriner Dergisi is the property of Firat Universitesiu, Saglik Bilimleri Enstitusu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this study, the in vitro effect of Rosmarinus officinalis L. (Rosemary) aqueous extract on rat gastric acid secretion was investigated. 24 male Sprague Dawley male rats were used in the study. Rats were euthanized by cervical dislocation in the presence of ketamine hydrochloride (Ketalar: 75 mg / kg) and xylazine (Rompun: 15 mg / kg) anesthesia. The isolated stomachs were left in the isolated organ bath for 1 hour for the incubation period. At the end of this period, the pH of the mucosal fluid obtained from the stomachs was evaluated as the basal pH. Histamine was then applied to the bath environment to induce stomach acid secretion and the pH values of the stomach contents were measured. Four different doses of Rosemary extract (150, 300, 600 and 1200 µg / ml) were applied to the bath separately and the pH of the stomach contents was recorded. In addition, Rosemary Extract's four separate doses were applied to the individual baths with Histamine and the pH values of the stomach contents were determined. The resulting pH values were compared to the basal pH and the PH values were calculated. In conclusion, the effect of 150 µg / ml dose of Rosemary extract on gastric acid secretion was not statistically significant and doses of 300, 600 and 1200 µg / ml increased stomach acid secretion statistically significantly. [ABSTRACT FROM AUTHOR]

Lichens are unity that commonly used in the alternative medicine. There are numerous studies on the biologic contents and activities of lichens. In this study, it has aimed that to investigate the effect of methanol extract obtained from Usnea longissima Ach lichen species on acid secretion in in vitro rat stomach. Our study were used 24 adult male Sprague- Dawley rats. Rats were euthanized under anesthesia. The stomach tissue was isolated and cleaned in a petri dish containing serosal solution. Emptying stomach contents was done with mucosal solution. The effects of the five different doses (0.012 μg/ml, 0.12 μg/ml, 1.2 μg/ml, 12 μg/ml, 120 μg/ml) of Usnea longissima Ach. methanol extract on gastric acid secretion were investigated in the presence and absence of antagonists of neuronal (acetylcholine) and paracrine (histamine) pathways in the isolated organ bath. In conclusion, it was obtained that different doses of Usnea longissima Ach. extract increased significantly the gastric acid secretion. It was arrive the opinion that of the gastric acid secretion enhancing effect of Usnea longissima Ach.'s is via the histamine mediated paracrine pathway. [ABSTRACT FROM AUTHOR]

Background/Aim. Impulse oscillometry (IOS) is a technique valid for measuring the lung function in obstructive lung diseases and bronchial provocation tests. However, no consensus exists for its use. The aim of the study was to assess impulse oscillometry sensitivity for detection of early airways changes during bronchial provocation testing and to compare with changes obtained with spirometry and bodyplethysmography in male army recruits. Methods. Male military recruits were submitted to bronchial provocation test with histamine by the aerosol provocation system. Out of 52 male military recruits subjected to attempts to make the diagnosis of asthma the study included 31 subjects with fall of forced expiratory volume in one second (FEV1) above 20%. The changes of impulse oscillometry were measured one step before and after provocation dose (PD) of histamine and compared with the changes of bodyplethysmography and spirometry. Results. The average age of male army recruits was 23.3 year. After bronchoprovocation there was an average increase of the total resistance at 5 Hz (R5) by 66.6%, resonant frequency (Fres) by 102.2%, Goldman index (AX) by 912.1%, the arway resistance (Raw) by 121.5%, and a decrease in reactance at 5 Hz (X5) by 132.1% and FEV1 by 25.6%. One step before the last inhaled of PD20 there was an average increase of 26.7% in R5, 24.1% in Fres, 85.3% in AX, 11.9% in Raw and a decrease in X5 by 26.9% and FEV1 by 4.3%. A correlation between impulse oscillometry and bodyplethysmography parameters was obtained. Conclusion. This paper demonstrates a sufficient sensitivity of impulse oscillometry to detect changes in airways, so it may play a complementary role in the diagnosis of asthma in male military recruits. [ABSTRACT FROM AUTHOR]

Background/Aim. Hyperreactivity of the airways caused by inflammation in asthmatics is the most important pathophysiological change. It represents a suitable ground that in the presence of risk factors and the drivers of asthma, asthmatic attack occurs. Atopic constitution is one of the most important risk factors for the development and expression of asthma. The aim of our study was to investigate the relationship between nonspecific airway hyperreactivity and atopic constituton in asthmatics. Methods. This retrospective analysis was conducted considering the results of nonspecific bronchoprovocative test with histamine, skin tests to inhalant allergens and total IgE levels in the serum of asthmatic patients with controlled bronchial asthma. The sample consisted of 162 asthmatics examined during one-year period. Results. The examinees were male asthmatic patients, aged between 18 and 30 years. We found that the examinees with a pronounced non-specific hyperreactivity had more significant skin reaction to inhaled allergens and higher levels of total IgE in serum. Conclusion. The results of our study show that the intensity of airway hyperresponsiveness to histamine in asthmatics is directly related to atopic constitution. [ABSTRACT FROM AUTHOR]

We report on a 59-year-old patient with a rash, hypotension and chest pain after eating tuna fish. A diagnosis of scombroid fish poisoning was made. It is a syndrome resembling an allergic reaction that occurs after eating fish of the Scombridae family contaminated with high levels of histamine. The authorities responsible for food safety should be immediately informed in order to investigate the event from their perspective, i.e. inspect selling premises, sample and test implicated food, as well as to take appropriate measures. Anaphylaxis rarely manifests as a vasospastic acute coronary syndrome, called Kounis syndrome. [ABSTRACT FROM AUTHOR]

In this study, by the parameters of validation a comparison of two high pressure liquid chromatography (PC) methods one of which utilizes coupled the FLED and the o-phthaldialdehyde derivatization method of Antoine et al. (1999) versus DAD coupled, benzoyl chloride derivatization method with a modification of Özdestan and Üren (2009) were made for the selection of sensitive and safe assay for the determination of histamine. Alive horse mackerels caught from the sea of Marmara in Kucukcekmece Istanbul were used for the limit of detection studies. For the other validation parameters, recovery, accuracy, precision and comparison of methods a certified reference material; FAPAS T2775QC were used. Results of the analyses indicated that the FLD coupled; o-phthaldialdehyde derivatization method the limit of quantification (LOQ) and recovery were 1.38 ppm and 100% respectively; and for DAD coupled; benzoyl chloride derivatization modified method these values were 10.66 ppm and 88% respectively. There was a significant difference between for both methods in t-test (p<0.5). According to the calculations of Horrat value; the reproducibility was normal for o-phthaldialdehyde method but found to be higher than the accepted limits in benzoyl chloride method. In conclusion, it is advised to choose FLD coupled o-phthaldialdehyde derivatization method for the safe and sensitive determination of histamine in fish and fish products. [ABSTRACT FROM AUTHOR]

Raoultella ornithinolytica is a species of gram-negative encapsulated and aerobic bacteria belonging to the family Enterobacteriaceae and is mainly found in fish. The most distinctive feature of this bacterium is the ability to convert histidine to histamine; therefore, the consumption of decomposing fish infected by R. ornithinolytica causes rashes, diarrhea, flushing, sweating and vomiting. This food poisoning is also called histamine fish poisoning; however, human infections with R. ornithinolytica are extremely rare and have so far only affected patients with diseases suppressing the immune system. The current case report describes for the first time sepsis with evidence of bloodstream infection by R. ornithinolytica in an immunocompetent male patient and the successful antibiotic treatment. [ABSTRACT FROM AUTHOR]

Introduction: Anaphylactic reaction is a most serious allergy where the symptoms may occur shortly after contact with an allergen and can get worse quickly. Therefore, timely and appropriate treatment is of crucial importance. Methods: In this article, we draw on evidence from publications on the subject of anaphylaxis treatment that we got by searching Google Scholar and PubMed databases. Used articles included: systematic reviews, case reports and randomised controlled trials. We also used World Allergy Organization guidelines for the management and the assessment of anaphylaxis. A literature search with the keywords “anaphylaxis treatment”, “anaphylactic shock”, and “allergy” identified a number of potentially eligible studies, of which 42 satisfied our eligibility criteria and were therefore included in this review. Topic: There was evidence regarding the optimum route, dose and site of adrenaline administration ,with the latest recommendations indicating the intramuscular route (i.m.) in the mid-outer thigh as the optimum treatment. We found studies suggesting the purpose of applying H1 and H2 antihistamines, systemic glucocorticosteroids, calcium and methylxantines to manage anaphylactic shock, recommended doses and mode of administration. With regard to treatment, we focused on acute rather than on long-term management. Further, we have taken into consideration the mechanisms, common triggers and clinical manifestations of anaphylaxis. Conclusion: Adrenaline represents the first choice drug and it is necessary to be applied as soon as possible. However, there is a controversy regarding the application of the other specified medications in this life-threatening condition. [ABSTRACT FROM AUTHOR]