Search

This study presents the direct measurement of proton transport along filamentous Desulfobulbaceae, or cable bacteria. Cable bacteria are filamentous multicellular microorganisms that have garnered much interest due to their ability to serve as electrical conduits, transferring electrons over several millimeters. Our results indicate that cable bacteria can also function as protonic conduits because they contain proton wires that transport protons at distances >100 μm. We find that protonic conductivity (σP) along cable bacteria varies between samples and is measured as high as 114 ± 28 μS cm-1 at 25 °C and 70% relative humidity (RH). For cable bacteria, the protonic conductance (GP) and σP are dependent upon the RH, increasing by as much as 26-fold between 60% and 80% RH. This observation implies that proton transport occurs via the Grotthuss mechanism along water associated with cable bacteria, forming proton wires. In order to determine σP and GP along cable bacteria, we implemented a protocol using a modified transfer-printing technique to deposit either palladium interdigitated protodes (IDP), palladium transfer length method (TLM) protodes, or gold interdigitated electrodes (IDE) on top of cable bacteria. Due to the relatively mild nature of the transfer-printing technique, this method should be applicable to a broad array of biological samples and curved materials. The observation of protonic conductivity in cable bacteria presents possibilities for investigating the importance of long-distance proton transport in microbial ecosystems and to potentially build biotic or biomimetic scaffolds to interface with materials via proton-mediated gateways or channels. [ABSTRACT FROM AUTHOR]

Background: Nocardia infections are rare infections in immunocompetent patients and occur mostly in immunocompromised individuals. Usually, nocardia affects skin, brain, and lungs, but in disseminated forms, which occurred mostly in immunocompromised patients, it can involve every organ. Nocardia sinusitis is extremely rare as our searches returned only a very few related studies. Case presentation: In this case report, we present, for the first time, a 55-year-old Iranian male patient diagnosed with chronic lymphocytic leukemia who was receiving chemotherapeutic drugs and developed a fever. Further laboratory tests and imaging revealed pansinusitis. Following rhinoendoscopy, the sinus mucosal biopsy pathology report showed sever inflammation accompanied by aggregation of filamentous thin-walled bacteria. The patient was treated with co-trimoxazole and meropenem. Following good clinical improvement the patient was discharged and advised to continue oral co-trimoxazole for 3 months. Conclusion: This case highlights that patients with febrile neutropenia should be assessed for rare infectious disease etiologies, especially those with chronic lymphocytic leukemia, as they have humeral immunodeficiency, and in the later stages of the disease, cellular immunodeficiency may also be involved. Therefore, a multisystem evaluation of patients with febrile neutropenia is necessary, particulary when no obvious source is identified in initial surveys, to uncover rare etiologies. [ABSTRACT FROM AUTHOR]

Salinity was considered to have effects on the characteristics, performance microbial communities of aerobic granular sludge. This study investigated granulation process with gradual increase of salt under different gradients. Two identical sequencing batch reactors were operated, while the influent of Ra and Rb was subjected to stepwise increments of NaCl concentrations (0–4 g/L and 0–10 g/L). The presence of filamentous bacteria may contribute to granules formed under lower salinity conditions, potentially leading to granules fragmentation. Excellent removal efficiency achieved in both reactors although there was a small accumulation of nitrite in Rb at later stages. The removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) in Ra were 95.31%, 93.70% and 88.66%, while the corresponding removal efficiencies in Rb were 94.19%, 89.79% and 80.74%. Salinity stimulated extracellular polymeric substances (EPS) secretion and enriched EPS producing bacteria to help maintain the integrity and stability of the aerobic granules. Heterotrophic nitrifying bacteria were responsible for NH 4 +-N and NO 2 −-N oxidation of salinity systems and large number of denitrifying bacteria were detected, which ensure the high removal efficiency of TN in the systems. [ABSTRACT FROM AUTHOR]

A 2-y-old, intact female, mixed-breed dog was presented to the veterinary hospital with abdominal distension, anemia, and lethargy following a chronic history of nonspecific gastrointestinal signs. CBC and serum biochemistry revealed moderate nonregenerative anemia with neutrophilia, hypoalbuminemia, hyperglobulinemia, hypoglycemia, decreased urea and creatinine, and hypercholesterolemia. Abdominal radiographs and ultrasound revealed a large heterogeneous mesenteric mass and ascites. Abdominocentesis confirmed septic peritonitis with filamentous bacteria. Fine-needle aspiration of the mass yielded pyogranulomatous inflammation and hyphae. An exploratory laparotomy revealed a large cranial abdominal mass with granulomas present throughout the abdominal cavity. Due to the poor prognosis and disseminated disease, the owner elected euthanasia. Postmortem and histologic examinations detected intralesional mycetomas and bacterial colonies within the mesenteric masses. 16S ribosomal RNA gene PCR and sequencing using formalin-fixed, paraffin-embedded sections identified Nocardia yamanashiensis, Nocardioides cavernae, and Nocardioides zeicaulis. Fungal culture, PCR, and sequencing confirmed Scedosporium apiospermum. Our report highlights the importance of molecular methods in conjunction with culture and histologic findings for diagnosing coinfections caused by infrequent etiologic agents. Additionally, we provide a comprehensive literature review of Scedosporium apiospermum infections in dogs. [ABSTRACT FROM AUTHOR]

The gut microbiota influences the reactivity of the immune system, and Parabacteroides distasonis has emerged as an anti-inflammatory commensal. Here, we investigated whether its lysate could prevent severe forms of neuroinflammation in experimental autoimmune encephalomyelitis (EAE) in mice and how this preventive strategy affects the gut microbiota and immune response. Lysate of anaerobically cultured P. distasonis (Pd lysate) was orally administered to C57BL/6 mice in four weekly doses. One week later, EAE was induced and disease severity was assessed three weeks after induction. Fecal microbiota changes in both vehicle- and Pd lysate-treated animals was analyzed by 16S V3–V4 amplicon sequencing and qPCR, antimicrobial peptide expression in the intestinal mucosa was measured by qPCR, and immune cell composition in the mesenteric and inguinal lymph nodes was measured by multicolor flow cytometry. Pd lysate significantly delayed the development of EAE and reduced its severity when administered prior to disease induction. EAE induction was the main factor in altering the gut microbiota, decreasing the abundance of lactobacilli and segmented filamentous bacteria. Pd lysate significantly increased the intestinal abundance of the genera Anaerostipes , Parabacteroides and Prevotella , and altered the expression of antimicrobial peptides in the intestinal mucosa. It significantly increased the frequency of regulatory T cells, induced an anti-inflammatory milieu in mesenteric lymph nodes, and reduced the activation of T cells at the priming site. Pd lysate prevents severe forms of EAE by triggering a T regulatory response and modulating T cell priming to autoantigens. Pd lysate could thus be a future modulator of neuroinflammation that increases the resistance to multiple sclerosis. [ABSTRACT FROM AUTHOR]

Depression is a serious cognitive disorder that results in significant and pervasive deficits in social behavior. These deficits can be traced back to the intricate interplay between social, psychological, and biological factors. Inflammatory depression, a treatment-resistant or non-responsive subtype of depression, may be related to the interaction between the gut microbiota and interleukin-17-producing CD4+ T cells (Th17 cells). The heterogeneity, plasticity, and effector role of Th17 cells in depression may be influenced by microbiota factors. Commensals-elicited homeostatic Th17 cells preserve the morphological and functional integrity of the intestinal barrier. In addition to pathogen-elicited inflammatory Th17 cells, commensal-elicited homeostatic Th17 cells can become conditionally pathogenic and contribute to the development of inflammatory depression. This review delves into the possible involvement of Th17 cells in inflammatory depression and examines the interplay between gut microbiota and either homeostatic or inflammatory Th17 cells. [ABSTRACT FROM AUTHOR]

A 10-month-old male guinea pig was presented with an area of alopecia and pruritus on the back. This progressed over 6 months to an ulcerated, painful skin mass. The aim of this report was to illustrate the unique cytological appearance of the Splendore-Hoeppli (SH) reaction, which has rarely been described in veterinary medicine. The mass was sampled using a fine-needle aspiration technique for cytological examination and bacteriological culture. Following cytological diagnosis and bacteriological results, the mass was surgically excised and histopathological examination was performed. Cytological examination of the mass revealed pyogranulomatous inflammation with filamentous bacteria identified by culture to be Actinomyces spp. The bacteria were often coated with a moderate amount of red to blue staining material, interpreted to represent the SH reaction. Histopathological examination confirmed the presence of the Splendore-Hoeppli phenomenon (SHP). The appearance of SH in histological specimens is well described in veterinary medicine, but the cytological appearance is not well recognized. This report illustrates well the cytological description of the material which should be recognized as a part of the immune response to insult, and not be mistaken as foreign material. [ABSTRACT FROM AUTHOR]

In this study, an anaerobic membrane bioreactor coupled with a complete nitrification and partial denitrification–anammox process (AnMBR–NF–PDA) was developed to efficiently remove both chemical oxygen demand (COD) and nitrogen. Precise control of raw water ratios was utilized to adjust the ratio of COD/NO3−–N, resulting in maximum nitrogen removal efficiency of 90.14% at a ratio of 3.44. Initially, specific anammox activity (SAA) increased with the proportion of raw water, peaking at 17.83 mg-N/(g-VSS·d) in stage II before decreasing. This variation was attributed to the significant presence of filamentous bacteria, especially "Acinetobacter" (13.58%–31.59%), which facilitated nitrite generation, supporting the nitrous oxide hypothesis in partial denitrification processes and enabling cross-feeding with AnAOB. Additionally, the average particle size of granular sludge increased from 300 to 528 µm under the influence of filamentous bacteria. Metagenomic analysis revealed an upsurge in genes associated with partial denitrification (NarG and NapA) as the COD/NO3–N ratio rose. The abundance of genes closely correlated with anammox (Hzs and Hdh) peaked during stage II, indicating the beneficial role of filamentous bacteria in the stable conversion of nitrite in PDA system. This study offers valuable insights into optimizing the synergistic metabolism and granulation processes involving filamentous bacteria and AnAOB, thereby laying the groundwork for the practical application of AnMBR coupled with anammox processes in wastewater treatment. [ABSTRACT FROM AUTHOR]

Actinomycetoma, a neglected tropical disease affecting the skin and soft tissues, is primarily caused by filamentous bacteria including Nocardia species. Here, we report a healthy 56-year-old man who has a one-year history of nodular lesions with seropurulent discharge on his right knee. Despite negative initial tissue culture, the sulfur granules that were partially acid-fast and Gram-positive branching filamentous rods were revealed in the tissue section. Repeated investigation identified the rare pathogen Nocardia wallacei, using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal ribonucleic acid (rRNA) gene sequencing. The patient was successfully treated with a six-month course of trimethoprim-sulfamethoxazole. This report describes a rare case of actinomycetoma due to N. wallacei, highlighting the challenges in diagnosis and the importance of accurate pathogen identification for the successful management of infection. The current literature regarding the causative agent will also be discussed. [ABSTRACT FROM AUTHOR]

The organic compound composition of wastewater, serves as a crucial indicator for the operational performance of activated sludge processes and has a major influence on the development of filamentous bulking in activated sludge. This study focused on the impact of typical soluble and slowly-biodegradable organic compounds, investigating the pathways through which these substrates affect the occurrence of filamentous bulking in systems operated under both high- and low-oxygen conditions. Results showed that slowly-biodegradable organic compounds lead to a concentrated distribution of microorganisms within flocs, with inward growth of filamentous bacteria. Both Tween-80 and granular starch treated systems exhibited a significant increase in protein content. The glucose system, utilizing soluble substrates, exhibited a markedly higher total polysaccharide content. Microbial communities in the Tween-80 and granular starch treated systems were characterized by a higher abundance of bacteria known to enhance sludge flocculation and settling, such as Competibacter, Xanthomonadaceae and Zoogloea. These findings are of high significance for controlling the operational performance and stability of activated sludge systems, deepening our understanding and providing a novel perspective for the improvement of wastewater treatment processes. [Display omitted] [ABSTRACT FROM AUTHOR]

The morphology is the consequence of evolution and adaptation. Escherichia coli is rod-shaped bacillus with regular dimension of about 1.5 μm long and 0.5 μm wide. Many shape-related genes have been identified and used in morphology engineering of this bacteria. However, little is known about if specific metabolism and metal irons could modulate bacteria morphology. Here in this study, we discovered filamentous shape change of E. coli cells overexpressing pigeon MagR, a putative magnetoreceptor and extremely conserved iron-sulfur protein. Comparative transcriptomic analysis strongly suggested that the iron metabolism change and iron accumulation due to the overproduction of MagR was the key to the morphological change. This model was further validated, and filamentous morphological change was also achieved by supplement E. coli cells with iron in culture medium or by increase the iron uptake genes such as entB and fepA. Our study extended our understanding of morphology regulation of bacteria, and may also serves as a prototype of morphology engineering by modulating the iron metabolism.

ABSTRACT Analyzing the relationship between cell morphology, rheological characteristics, and production dynamics of cultivations with filamentous microorganisms is a challenging task. The complex interdependencies and the commonly low reproducibility of heterogeneous cultivations hinder the bioprocess development of commercially relevant production systems. The present study aims to characterize process parameters in Actinomadura namibiensis shake flask cultures to gain insights into relationships between culture behavior and rheological characteristics during salt‐enhanced labyrinthopeptin A1 production. Plate–plate (PP) and vane–cup rheometer measurements of viscous model fluids and culture broths are compared, revealing a more uniform distribution of broth when measured with the PP system. Additionally, rheological characteristics and culture performance of A. namibiensis cultures are evaluated using online data of the specific power input and the oxygen transfer rate. It is demonstrated that salt‐enhancement labyrinthopeptin A1 production by the addition of 50 mM (NH4)2SO4 increases the apparent viscosity of the A. namibiensis culture by four‐fold and significantly reduces the reproducibility of the culture resulting in a 46 h difference in lag‐phase duration. This approach demonstrates that the culture behavior of complex filamentous cell morphologies is challenging to decipher, but online monitoring of rheology and oxygen transfer can provide valuable insights into the cultivation dynamics of filamentous microbial cultures. [ABSTRACT FROM AUTHOR]

Airport de-icing has been linked with the growth of undesirable river biofilms (URBs, formerly "sewage fungus"), a manifestation of organic pollution causing long-term ecological damage to watercourses. URBs are a polymicrobial community, with one key taxon standing out in literature: Sphaerotilus natans , a filamentous bacterium also found in sewage treatment and activated sludges. An industry often implicated in causing URBs is airport de-icing, with large biofilms often developing downstream of airport discharges in winter months. However, it is not yet clear which de-icers may cause URBs and how they influence growth. Therefore, specific objectives were to (i) determine which freeze-point depressants (FPDs) can be utilized by S. natans ; (ii) examine differences in the growth kinetics between FPDs; and (iii) compare pure-FPDs to commercial airport de-icers (CADs) as carbon sources, to determine impacts of additives. This study employed a turbidimetric micro-batch culture design to conduct microbial growth experiments, using S. natans and a minimal medium supplemented with airport de-icer as the carbon source. Equimolar carbon concentrations were used to compare the effects of common FPDs and CADs – each containing a specific FPD. Growth was assessed via optical density (OD600) measurements, from which time-to-detection, maximum rate of change, and maximum optical density were derived and kinetics inferred. S. natans was found to grow effectively on all FPDs tested, although the microbial yield was heavily dependent on the carbon concentration for all FPDs and CADs. Sodium acetate generated the quickest growth, with the lowest TTD (lag-time) for all but the lowest concentrations tested. Propylene glycol produced the greatest maxOD (total growth), whereas ethylene glycol had a higher limiting concentration for maxROC (growth rate). The mixture of compounds and additives in commercial products did not significantly impact the growth of S. natans. This research provides evidence from controlled laboratory experiments that airport de-icers support the growth of S. natans. The differences in growth kinetics observed for the FPDs and CADs could inform improved mitigation or treatment to reduce the incidence and ecological impacts of URBs. [ABSTRACT FROM AUTHOR]

Aims Glucosaminoglucan (β-1,4-linked glucose and glucosamine) produced by a mixotrophic sulfur-oxidizing bacterium, Thiothrix nivea , is a useful cellulose-aminating agent. Lithotrophic and mixotrophic glucosaminoglucan production were examined using fed-batch techniques. Methods and results A jar fermenter was used for the fed-batch cultivation. Glucosaminoglucan was extracted from T. nivea using diluted HCl. Lithotrophic growth was detected by feeding with Na2S as the energy source, and 12 mg l−1 of glucosaminoglucan was obtained. In contrast, no growth was observed with Na2S2O3. Similarly, mixotrophic growth in the presence of acetic acid was promoted by Na2S, whereas Na2S2O3 had no effect. When acetic acid and Na2S were added, 470 mg l−1 of glucosaminoglucan was obtained. Conclusions Thiothrix nivea was cultured, and glucosaminoglucan was produced lithotrophically using Na2S for feeding. Na2S is also indispensable for mixotrophic growth and glucosaminoglucan production, indicating that sulfide oxidation pathways control the TCA cycle. The involvement of the SOX pathway (for thiosulfate oxidation) in the activation of energy metabolism is doubtful because neither lithotrophic nor mixotrophic growth was promoted by Na2S2O3. Based on these results, we assumed that T. nivea is facultatively mixotrophic [lithotrophic growth is possible in addition to organotrophic growth in the presence of sulfide (Na2S)], rather than obligately mixotrophic. [ABSTRACT FROM AUTHOR]

Osteoarthritis is a common cause of morbidity and mortality in geriatric gazelles. Propionibacterium australiense has been reported as a cause of systemic granulomas in cattle, but there are no descriptions of this bacteria infecting other species nor causing osteoarthritis, to our knowledge. An 8-y-old, castrated male, sand gazelle (Gazella leptoceros leptoceros) was managed for chronic, intermittent, progressive osteoarthritis of the right tarsus. Serial biopsies revealed pyogranulomatous dermatitis with intralesional bacteria. Serial diagnostic imaging identified osseous and soft tissue proliferation with draining tracts. Treatments over 1 y included broad-spectrum antibiotics, anti-inflammatories, joint debridement, and infusion with platelet-rich plasma and stem cells. Despite therapy, lameness persisted, azotemia developed, and subsequently, the animal was euthanized. On postmortem examination, the periarticular tissue of the right tarsus was markedly expanded by pyogranulomas and fibrosis. Histologically, the synovium, joint capsule, and overlying soft tissues were markedly expanded by pyogranulomas and numerous gram-positive and acid-fast–negative filamentous bacteria surrounded by Splendore–Hoeppli material. Within the joint, there was regionally extensive cartilage ulceration, osteonecrosis, osteolysis, and pannus formation. PCR assay of affected formalin-fixed, paraffin-embedded tissue amplified segments of 16S rRNA and β subunit of bacterial RNA polymerase (rpoB) genes with 99.7% and 95.6% identity to P. australiense. This bacterium should be considered a differential for chronic pyogranulomatous osteoarthritis in gazelles. [ABSTRACT FROM AUTHOR]

Salmonella can cause infection in poultry and lead to intestinal lesions, and food-borne infections in humans through food transmission. Salmonella enters the intestines of poultry through oral ingestion, adheres to and invades intestinal epithelial cells, uses M cells and macrophages to achieve intracellular survival and reproduction, and causes intestinal lesions and systemic invasion. Virulence genes and plasmids are regulated during this process, including SPI-1 and SPI-2 encoded type IE secretion systems and induced filamentous formation that help bacteria evade host defenses. Birds enhance their ability to clear Salmonella by regulating the synergistic effect of innate immune system and adaptive immune system. In this paper, the etiology, transmission route, pathogenesis and immune response mechanism of Salmonella were described, in order to provide reference for further study of Salmonella infection in poultry. [ABSTRACT FROM AUTHOR]

Strain C29T, a Gram-staining-negative, straight rod occurring singly, in pairs or short chains, was isolated from floating filamentous biomass of the Uruguay River. The strain was catalase and oxidase positive, chemoorganotrophic, strictly aerobic, non-motile, and grew at pH 6.0–9.0, 15–45 °C, and 0–0.5% (w/v) NaCl. Polyhydroxybutyrate was accumulated in nutrient-limited conditions. Phylogenetic analysis based on the 16S rRNA gene revealed that strain C29T had the highest sequence similarity with Leptothrix discophora SS-1T (97.82%), Ideonella livida TBM-1T (97.82%), Vitreoscilla filiformis L1401-2T (97.52%), Sphaerotilus sulfidivorans D-501T (97.50%) and Sphaerotilus natans DSM 6575T (97.46%). Other type strains with validly published names had similarities below 97.46%. Further phylogenomic analysis showed that strain C29T was affiliated to the family Sphaerotilaceae. Average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values with its phylogenetic relatives were lower than 91 and 41%, respectively, revealing that strain C29T represented a new species. The DNA G + C content of strain C29T was 70.9%. The annotation of the genome of the novel strain shows it possessed genes for the degradation of aromatic compounds. It also contained genes that encode sigma factors involved in response regulation of stress resistance, which is an important function for adaptation and survival in natural niches. Based on the results of the phylogenetic and phenotypic analyses, we propose that strain C29T represents a novel species, for which the name Sphaerotilus uruguayifluvii sp. nov. is proposed. The type strain is C29T (= CCM 9043T = DSM 113250T). [ABSTRACT FROM AUTHOR]

Actinomycosis is a rare infection that involves soft and bone tissues caused by gram-positive, anaerobic filamentous bacteria, usually Actinomyces israelii. About 3% of all cases affect children. Not uncommonly, actinomycosis is initially diagnosed as neoplasm or other granulomatous diseases. Paediatric actinomycosis affecting the jaws is rare. To date, 20 and 1 case affecting the mandible and maxilla, respectively, have been reported. A 4-year-old girl was referred presenting an extensive symptomatic ulcerative lesion with bone exposure in the posterior region of the right maxilla two months ago. One month before, she has been diagnosed with scarlet fever. The lesion progressed, damaging the supporting periodontal tissue. After surgical removal of necrotic bone and deciduous molar, the microscopy revealed actinomycosis, which showed excellent healing after local treatment with hydrogen peroxide solution and adequate oral hygiene. Although actinomycosis of the jaws affecting pediatric patients is rare, paediatric dentists must be aware of their clinicopathological characteristics, with therapeutic and prognostic impact. [ABSTRACT FROM AUTHOR]

Filamentous bacteria belonging to the Streptomyces genus are well known for their exceptional capacity to generate a diverse range of bioavailable secondary metabolites. In this work, an inoculum for fermentation was created using Streptomyces sp., which was isolated from Yanbu City, Saudi Arabia. It was 99% identical to Streptomyces spororaveus AD 4, according to 16S ribosomal RNA sequence homology studies. After using ethyl acetate to extract the fermented broth, the crude extract's purity was checked using high-performance liquid chromatography (HPLC). Following the purification stages, three pure bioactive fractions were isolated and given the names A, B, and C, with retention times of 2.251 ± 0.3 min, 4.342 ± 0.3 min, and 5.988 ± 0.3 min, respectively. Additionally, the spectroscopic examinations of these three compounds, including their GC-MS data, were used to determine their chemical structures by comparing them to published reference data. Additionally, the three fractions exhibit potent antimicrobial activity against Salmonella typhi ATCC 14028, Candida albicanc, Aspergillus niger, Klebsiella pneumoniae, Pseudomonas aeruginosa ATCC 9027, Staphylococcus epidermidis, Micrococcus luteus, E. coli ATCC 8739, and B. subtilis ATCC 6633. Also, the cytotoxicity of the bioactive fractions was assessed using the Hct-116 line. Of the three fractions, fraction C had the lowest cytotoxicity, with an IC50 value of 224.57 ± 5.82, followed by fraction B at 238.82 ± 2.57 and fraction A at 341.28 ± 1.69. [ABSTRACT FROM AUTHOR]

Cable bacteria exhibit a unique metabolism involving long‐distance electron transport, significantly impacting elemental cycling in various sediments. These long filamentous bacteria are distributed circumglobally, suggesting an effective mode of dispersal. However, oxygen strongly inhibits their activity, posing a challenge to their dispersal through the water column. We investigated the effective dispersal of marine cable bacteria in a compartmentalised microcosm experiment. Cable bacteria were grown in natural 'source' sediment, and their metabolic activity was recorded in autoclaved 'destination' cores, which were only accessible through oxygenated seawater. Colonisation occurred over weeks, and destination cores contained only one cable bacterium strain. Filament 'snippets' (fragments with a median size of ~15 cells) accumulated in the microcosm water, with about 30% of snippets attached to sediment particles. Snippet release was also observed in situ in a salt marsh creek. This provides a model for the dispersal of cable bacteria through oxygenated water: snippets are formed by filament breakage in the sediment, released into the overlying water and transported with sediment particles that likely offer protection. These insights are informative for broader theories on microbial community assembly and prokaryotic biogeography in marine sediments. [ABSTRACT FROM AUTHOR]

Sludge disintegration is an environmental and industrial challenge that requires intensive research and technological development. Sludge has a complex structure with a high yield of various chemical and biological compounds. Anaerobic digestion is the most commonly used process for sludge disintegration to produce biogas, detoxify sludge, and generate biosolids that can be used in agriculture. Biological cell lysis is the rate‐limiting cell lysis. This review discusses the application of sonolysis as a sludge pretreatment for enhanced anaerobic digestion via three combined processes: thermal destruction, hydrochemical shear forces, and radical oxidation. The mechanistic pathways of sono‐pretreatment to enhance biogas, sludge‐enhanced dewatering, activation of filamentous bacteria, oxidation of organic pollutants, release of heavy metals, reduction of bulking and foaming sludge, and boosting ammonia‐oxidizing bacterial activity are discussed in this review. This article also discusses the use of ultrasound in sludge disintegration, highlighting its potential in conjunction with Fenton and cation‐binding agents, and reviews common large‐scale sonoreactors available on the market.. [ABSTRACT FROM AUTHOR]

Filamentous bacteria are one of the main components of activated sludge microorganisms, which not only support flocculent bacteria in forming sludge flocs but also easily lead to sludge bulking. This article summarizes the high-throughput sequencing technology and database construction that integrates the FISH probe and 16s rRNA gene of filamentous bacteria. The detection frequency and abundance were statistically analyzed to determine the core filamentous bacteria. The filamentous bacteria searchable in the NCBI database belong to six phyla and 42 genera, with a total of 94 species. The core filamentous bacteria in the expanded activated sludge include Microthrix parvicella (M. parvicella), type 0092, Mycobacterium fortuitum, etc. The physiological ecology of the core filamentous bacteria is discussed in detail, aiming to establish targeted sludge expansion control strategies, reduce the incidence and harm of sludge expansion, and propose that future research needs to promote further development in the field of environmental microbiology. [ABSTRACT FROM AUTHOR]

Background: Nocardiosis, a rare but serious infection caused by Grampositive, aerobic actinomycetes, primarily affects immunocompromised individuals. This report highlights an uncommon case of pulmonary nocardiosis in an immunocompetent patient leading to acute hypoxic respiratory failure. Case Presentation: A 45-year-old male gardener with no significant past medical history presented with acute dyspnea, pleuritic chest pain, fever, night sweats, and weight loss. Physical examination revealed respiratory distress and hypoxia. Imaging showed bilateral alveolar infiltrates and cavitary lesions. Sputum analysis identified Gram-positive branching filamentous bacteria, and Nocardia species were cultured. HIV test was negative. Management: The patient was admitted to the ICU and treated with high-flow oxygen therapy and empirical antibiotics (trimethoprim-sulfamethoxazole [TMP-SMX] and meropenem). Upon culture results, TMP-SMX was continued, and meropenem was discontinued. The patient showed significant improvement and was discharged on oral TMP-SMX for a six-month course. Discussion: This case underscores the importance of considering pulmonary nocardiosis in acute respiratory failure, even in immunocompetent patients. Early diagnosis and appropriate treatment are crucial for favorable outcomes. Conclusion: Pulmonary nocardiosis can cause severe respiratory symptoms in otherwise healthy individuals. Prompt recognition and treatment are essential to manage this potentially life-threatening condition effectively. [ABSTRACT FROM AUTHOR]

Ozone is a molecule that has gained increasing interest in recent years by food industries for sanitization of food-grade surfaces. Compared to chemical sanitizers such as chlorine, hydrogen peroxide, or peracetic acid, ozone shows undeniable advantages, such as the absence of by-products that should affect human health or the possibility of generating it when needed. Therefore, the aim of this paper was the assessment of the resistance to ozonized water of two pathogenic bacteria (Listeria monocytogenes, Salmonella) and of three airborne food-spoiling fungi (Aspergillus brasiliensis, Hyphopichia burtonii, and Penicillium nordicum) inoculated on stainless steel tiles and treated in static conditions with 1 to 6 mg L−1 (pathogens) or 8.5 mg L−1 (filamentous fungi). Ozonized water gave different results based on the tested microorganisms: pathogenic bacteria proved markedly more sensible to ozone than filamentous fungi, even if great differences were observed at inter- and intra-specific levels for both categories of microorganisms. Nevertheless, the non-linear inactivation kinetics of the studied strains made the calculation of a punctual F-value difficult, so in industrial practice, adequate tailoring of the treatments to be applied, based on the registered extrinsic factors and the industrial bio-burden, would be appropriate. [ABSTRACT FROM AUTHOR]

The glycoside hydrolase family 3 (GH3) β-glucosidases from filamentous fungi are crucial industrial enzymes facilitating the complete degradation of lignocellulose, by converting cello-oligosaccharides and cellobiose into glucose. Understanding the diverse domain organization is essential for elucidating their biological roles and potential biotechnological applications. This research delves into the variability of domain organization within GH3 β-glucosidases. Two distinct configurations were identified in fungal GH3 β-glucosidases, one comprising solely the GH3 catalytic domain, and another incorporating the GH3 domain with a C-terminal fibronectin type III (Fn3) domain. Notably, Streptomyces filamentous bacteria showcased a separate clade of GH3 proteins linking the GH3 domain to a carbohydrate binding module from family 2 (CBM2). As a first step to be able to explore the role of accessory domains in β-glucosidase activity, a screening system utilizing the well-characterised Aspergillus niger β-glucosidase gene (bglA) in bglA deletion mutant host was developed. Based on this screening system, reintroducing the native GH3-Fn3 gene successfully expressed the gene allowing detection of the protein using different enzymatic assays. Further investigation into the role of the accessory domains in GH3 family proteins, including those from Streptomyces, will be required to design improved chimeric β-glucosidases enzymes for industrial application. [ABSTRACT FROM AUTHOR]

Organisms display an immense variety of shapes, sizes, and reproductive strategies. At microscopic scales, bacterial cell morphology and growth dynamics are adaptive traits that influence the spatial organization of microbial communities. In one such community--the human dental plaque biofilm--a network of filamentous Corynebacterium matruchotii cells forms the core of bacterial consortia known as hedgehogs, but the processes that generate these structures are unclear. Here, using live-cell time-lapse microscopy and fluorescent D-amino acids to track peptidoglycan biosynthesis, we report an extraordinary example of simultaneous multiple division within the domain Bacteria. We show that C. matruchotii cells elongate at one pole through tip extension, similar to the growth strategy of soil-dwelling Streptomyces bacteria. Filaments elongate rapidly, at rates more than five times greater than other closely related bacterial species. Following elongation, many septa form simultaneously, and each cell divides into 3 to 14 daughter cells, depending on the length of the mother filament. The daughter cells then nucleate outgrowth of new thinner vegetative filaments, generating the classic "whip handle" morphology of this taxon. Our results expand the known diversity of bacterial cell cycles and help explain how this filamentous bacterium can compete for space, access nutrients, and form important interspecies interactions within dental plaque. [ABSTRACT FROM AUTHOR]

Compost is a natural and sustainable way to improve soil fertility and enhance plant growth. Moringa leaves have high mineral, cytokinin, and vitamin content which are useful for growth so that they can be used as organic fertilizer. Azotobacter and Streptomyces are from soil and have many biological activities. This study aimed to detect the importance of bioagents formula with Moringa Compost (MC) to enhance plant growth in poor sterile soil and plants were irrigated with half strength of Hoagland nutrient solution. Moringa leaves were collected and cleaned, and organic compost was prepared and analyzed for microbial and chemical composition. The prepared MC was rich in nitrogen and minerals and had high content of bacteria and fungi. The two bioagents used were isolate MB5 and MB11 which were characterized and molecular identified as Azotobacter chroococcum MB5 and Streptomyces griseus MB11. The free-living A. chroococcum can fix atmospheric nitrogen while Streptomyces is a filamentous bacterium with a high ability to produce secondary metabolites. The addition of 20% MC to soil increased soil EC and microbial counts compared to MC-free soil. Moreover, inoculation of soil with either AZ or ST increased the microbial counts and soil EC and the clearest increase was in the case of inoculation of soil with MC+AZ+ST. It also found that MC extract alone with the bacterial filtrates increases seed germination of Phaseolus vulgaris L. (common bean), which is a herbaceous annual worldwide plant, grown for its edible dry seeds or green unripe pods. In this regard, inoculation of soil with inoculum of both A. chroococcum MB5, and S. griseus MB11, in the presence of MC has the most pronounced effect and enhances both the growth, fresh and dry weights, leaf number, plant height, and root length of P. vulgaris grown under greenhouse conditions for one month and chemical content of the plant protein carbohydrates, P, N, Ca++ and K+. In conclusion, the combined application A. chroococcum MB5 and S. griseus MB11, as a biofertilizers with Moringa compost is recommended to enhance P. vulgaris growth. The use of these biofertilizers can reduce the use of chemical fertilizers, which can have detrimental effects on soil and the environment. Therefore, further research on the inoculation and application of these microorganisms with MC is essential for sustainable agriculture. [ABSTRACT FROM AUTHOR]

hermodynamics has predicted many different kinds of microbial metabolism by determining which pairs of electron acceptors and donors will react to produce an exergonic reaction (a negative net change in Gibbs free energy). In energy-limited environments, such as the deep subsurface, such an approach can reveal the potential for unexpected or counter-intuitive energy sources for microbial metabolism. Up until recently, these thermodynamic calculations have been carried out with the assumption that chemical species appearing on the reactant and product side of a reaction formula have a constant concentration, and thus do not count towards net concentration changes and the overall direction of the reaction. This assumption is reasonable considering microorganisms are too small (~1 μm) for any significant differences in concentration to overcome diffusion. However, recent discoveries have demonstrated that the reductive and oxidative halves of reactions can be separated by much larger distances, from millimetres to centimetres via conductive filamentous bacteria, mineral conductivity, and biofilm conductivity. This means that the concentrations of reactants and products can indeed be different, and that concentration differences can contribute to the net negative change in Gibbs free energy. It even means that the same redox reaction, simultaneously running in forward and reverse, can drive energy conservation, in an ElectroMicrobiological Concentration Cell (EMCC). This paper presents a model to investigate this phenomenon and predict under which circumstances such concentration-driven metabolism might take place. The specific cases of oxygen concentration cells, sulfide concentration cells, and hydrogen concentration cells are examined in more detail. [ABSTRACT FROM AUTHOR]

We investigated suitable culture conditions for the production of the blue pigment phycocyanin (PC) from the unique filamentous cyanobacteria Pseudanabaena sp. ABRG5-3 and Limnothrix sp. SK1-2-1. White, green, or red LED irradiation at 30 μmol photons/m2/s was effective for phycocyanin production when compared with Arthrospira platensis (Spirulina) sp. NIES-39, which is generally grown under high light irradiation. To investigate the safety of the cyanobacteria, ABRG5-3 cells were subjected to Ames (reverse mutation) tests and single oral-dose rat studies, which revealed non-mutagenic and non-toxic properties. When three purified phycocyanins (abPC, skPC, and spPC) were subjected to agarose gel electrophoresis, they showed different mobility, indicating that each phycocyanin has unique properties. abPC exhibited strong antiglycation activities as novel function. • We assessed cultivation conditions and phycocyanin production of 3 cyanobacteria. • We tested various light irradiation conditions in terms of quality and intensity. • We found that ABRG5-3 cells are non-mutagenetic and non-toxic. • Each phycocyanin has unique properties depending on bacterial species. • The phycocyanins showed strong antiglycation activity. [ABSTRACT FROM AUTHOR]

Filamentous bacteria and fungi are attracting increasing attention due to their medicinal value. Among these microorganisms, Actinomadura namibiensis has attracted great interest due to its ability to produce Labyrinthopeptin A1 with antiviral activity. In order to increase productivity and shorten the development cycle, recent studies have shown that the production of Labyrinthopeptin A1 can be enhanced by optimising the cultivation processes of filamentous pellets with the help of morphology engineering techniques including the appropriate preparation of the culture medium and the flow conditions for agitation, as well as the contact mechanisms during the flow‐induced movements of the pellets. From a mechanical point of view, contact energy is positively correlated with productivity and is determined by the mechanical and morphological properties of the pellets, which have a string influence on the stresses, contact frequency and structure for mass transfer of nutrients. To gain a deeper understanding of the stress‐induced pellet growth mechanisms during cultivation, experimental characterisation of Actinomadura namibiensis pellets was performed using a micromechanical setup. Considering that filamentous pellets exhibit an irregular geometry with tightly intertwined and branched hyphal networks, the measured force responses of cyclic compression experiments were analysed in correlation with the morphological properties. In addition, the process‐dependent mechanical behaviour was investigated by comparing the mechanical behaviour at different cultivation times. The results obtained provide sufficient information to propose a contact model of the pellets and to derive the process‐dependent material parameters for further numerical simulations of the cultivation process. [ABSTRACT FROM AUTHOR]

In this study, the antibacterial activity of 10 honeys at three concentrations 50%, 25%, and 12.5% was tested against two G- and two G+ strains, also the microbiological quality of the honeys in terms of the representation of total count of bacteria, coliforms, yeasts, and filamentous fungi was evaluated, and micromycetes to the species level were identified. Antibacterial activity of the honeys was assayed using well diffusion method, determination of microbial groups by the pour plate method and diversity of mycobiota in honey according to macro- and micromorphological characteristics. Results showed the antibacterial effects of Slovak honey collected from the Spiš region, against bacterial strains Escherichia coli, Pseudomonas fluorescens, Enterococcus faecalis, and Staphylococcus aureus, which are among the most common bacteria responsible for nosocomial infections. We found that honeydew honey was very effective against E. coli and S. aureus, rapeseed honey against P. fluorescens, and mixed honey (no. 10) against E. faecalis. Staphylococcus aureus was the most susceptible bacteria tested for all honeys. The presence of both yeasts and molds was detected in 3 honey samples at concentrations ranging from <4x101 to 3.6x101 CFU/g, while the total count of bacteria was detected in 9 samples at concentrations ranging from 2.3x101 to 3.6x102 CFU/g (in 1 sample, the occurrence of microorganisms was not recorded even at the lowest dilution of 10-1). Coliforms were not isolated. A total of 3 strains belonging to the Aspergillus section Nigri were identified. The microbiological analyses of the samples indicates that the honeys were produced, processed and stored in accordance with the rules of good hygiene practice. [ABSTRACT FROM AUTHOR]

Sulfidic caves support diverse and abundant subterranean communities, including numerous endemic species and complex food webs, though the full extent of species diversity and resource utilization in these ecosystems remains largely unexplored. This paper presents the results of biological surveys conducted from 2023 to 2024 in Sulfur Cave, located in the Vromoner Canyon on the Greek–Albanian border, focusing on microbial, vertebrate, and invertebrate communities and investigating the structure of the subterranean food web. The microbial communities from the different biofilms are dominated by chemosynthetic sulfur-oxidizing microorganisms, specifically filamentous bacteria such as Thiotrix and Beggiatoa. Two species of fish, an eel (Anguilla sp.) and a Cyprinid (Alburnoides sp.), and six bat species from three families (Rhinolophidae, Miniopteridae, and Vespertilionidae) were documented. The invertebrate fauna includes five aquatic species, 25 terrestrial species, and four amphibiotic species. Among these, eight species are endemic, and seven species exhibit troglomorphic traits. Stable isotope analysis showed light carbon and nitrogen values for the terrestrial and aquatic invertebrates, suggesting that subterranean communities rely on food produced in situ by chemoautotrophic microorganisms. Our results identified cave areas of significant biological relevance and provided reference data to inform conservation actions aimed at preserving the biodiversity of this sulfidic cave. [ABSTRACT FROM AUTHOR]

The vertical stratification of oxygen concentration in deep reservoirs impacts nutrient cycling and ecosystem biodiversity. The Serra da Mesa reservoir, the largest in Brazil, was studied to evaluate the structure and production of the prokaryote community at five depths. Using 3H leucine incorporation and fluorescent in situ hybridization (FISH), the study focused on different depths near the dam, particularly within the euphotic zone. The water column was characterized into oxic, transitional, and hypoxic layers based on dissolved oxygen concentration. The highest densities and biomasses of prokaryotes were found at the euphotic zone's depth limit, where bacterial production was low, suggesting inactive or slow-growing bacteria. Cell size differences and filamentous bacteria presence near the surface were observed, likely due to varying predation pressures. Prokaryote community composition differed across depths. At the subsurface level, with high dissolved organic carbon, alphaproteobacteria, betaproteobacteria, and Cytophaga–Flavobacter had similar densities, but the lowest bacterial biomass was recorded. The highest dissolved oxygen concentration depth had the lowest bacterial density, dominated by alphaproteobacteria and gammaproteobacteria. The study revealed that prokaryotic community structure and production vary with depth, indicating that microbial participation in layer dynamics is differentiated, with variations in abundance and distribution linked to oxygen concentrations. [ABSTRACT FROM AUTHOR]

Cable bacteria are filamentous bacteria that couple the oxidation of sulfide in sediments to the reduction of oxygen via long-distance electron transport over centimeter distances through periplasmic wires. However, the capability of cable bacteria to perform extracellular electron transfer to acceptors, such as electrodes, has remained elusive. In this study, we demonstrate that living cable bacteria actively move toward electrodes in different bioelectrochemical systems. Carbon felt and carbon fiber electrodes poised at +200 mV attracted live cable bacteria from the sediment. When the applied potential was switched off, cable bacteria retracted from the electrode. qPCR and scanning electron microscopy corroborated this finding and revealed cable bacteria in higher abundance present on the electrode surface compared with unpoised controls. These experiments raise new possibilities to study metabolism of cable bacteria and cultivate them in bioelectrochemical devices for bioelectronic applications, such as biosensing and bioremediation. [ABSTRACT FROM AUTHOR]

Segmented filamentous bacteria (SFB) are members of the commensal intestinal microbiome. They are known to contribute to the postnatal maturation of the gut immune system, but also to augment inflammatory conditions in chronic diseases such as Crohn's disease. Living primary tissue slices are ultrathin multicellular sections of the intestine and provide a unique opportunity to analyze tissue‐specific immune responses ex vivo. This study aimed to investigate whether supplementation of the gut flora with SFB promotes T helper 17 (Th17) cell responses in primary intestinal tissue slices ex vivo. Primary tissue slices were prepared from the small intestine of healthy Taconic mice with SFB‐positive and SFB‐negative microbiomes and stimulated with anti‐CD3/CD28 or Concanavalin A. SFB‐positive and ‐negative mice exhibited distinct microbiome compositions and Th17 cell frequencies in the intestine and complex microbiota including SFB induced up to 15‐fold increase in Th17 cell‐associated mediators, serum amyloid A (SAA), and immunoglobulin A (IgA) responses ex vivo. This phenotype could be transmitted by co‐housing of mice. Our findings highlight that changes in the gut microbiome can be observed in primary intestinal tissue slices ex vivo. This makes the system very attractive for disease modeling and assessment of new therapies. [ABSTRACT FROM AUTHOR]

The discovery of cable bacteria has revolutionized our understanding of long-distance biological electron transfer and has provoked the study of their distribution. Cable bacteria are widely distributed in aquatic environments and exhibit superior adaptations at the oxic–anoxic interface. The physiology and available genomes of cable bacteria have provided insights into the factors that control cable bacteria distribution, facilitating the anticipation of their occurrence and management of their ecological benefits. Cable bacteria create redox gradients, alter sediment geochemistry and nutrient cycling, influence microbial activity and networks, and alleviate multiple anthropogenic problems. The impact of cable bacteria can extend to the atmosphere, and their ecological significance may be more far-reaching than was previously believed. Cable bacteria have been identified and detected worldwide since their discovery in marine sediments in Aarhus Bay, Denmark. Their activity can account for the majority of oxygen consumption and sulfide depletion in sediments, and they induce sulfate accumulation, pH excursions, and the generation of electric fields. In addition, they can affect the fluxes of other elements such as calcium, iron, manganese, nitrogen, and phosphorous. Recent developments in our understanding of the impact of cable bacteria on element cycling have revealed their positive contributions to mitigating environmental problems, such as recovering self-purification capacity, enhancing petroleum hydrocarbon degradation, alleviating phosphorus eutrophication, delaying euxinia, and reducing methane emission. We highlight recent research outcomes on their distribution, state-of-the-art findings on their physiological characteristics, and ecological contributions. [ABSTRACT FROM AUTHOR]

Soft drinks are industrial intermediate chemicals found in wastewater and are among the most significant environmental pollutants. Up-flow anaerobic sludge blanket (UASB) reactors are used to treat soft drink wastewater, offering high-volume loading capacity, optimal grain deposition, and the ability to bear impact loads. The use of support materials can enhance biological productivity and expedite the UASB start-up period. In this study, bone powder was utilized as a support material in a UASB reactor to remove contaminants from wastewater. During the 70-day sludge adaptation period, the treatment reactor achieved a 93% reduction in COD, while the control reactor achieved a 65% reduction. Biogas production was higher in the bone powder UASB reactor (1750 mL/d) compared to the control UASB reactor (1100 mL/d), and the bone powder UASB reactor demonstrated greater resistance to shock loading. The improvement in sludge settling, shear strength, and higher biological activity in the bone powder UASB reactor was attributed to the formation of large granular sludge. The size of the granular sludge increased further with the colonization of filamentous bacteria at the irregular levels of bone powder. [ABSTRACT FROM AUTHOR]

Nocardiosis is a rare systemic infection caused by aerobic, Gram-positive filamentous bacteria that belong to the genus Nocardia , typically affecting immunocompromised individuals but can also manifest in immunocompetent people. We report a case of pulmonary nocardiosis caused by Nocardia arthritidis in a 56-year-old female with a history of recurrent respiratory illness. The patient presented with a persistent cough, on-and-off fever, and a history of pulmonary tuberculosis. Sputum analysis revealed Gram-positive beaded branching filamentous bacilli, confirmed as N. arthritidis through molecular methods. Treatment with cotrimoxazole and ceftriaxone led to symptomatic improvement. [ABSTRACT FROM AUTHOR]

SUMMARY: Cyanobacteria are ancient organisms that contribute significantly to primary production in aquatic ecosystems. Although they are a group of microbes present in a wide range of environments, their diversity on anthropogenic substrates, namely microplastics, is still largely unknown. In the present study, we describe a new genus and species of cyanobacteria from the Nodosilineaceae family. Strains were isolated from foam microplastic particles obtained from beach sediments facing the Singapore Strait. This species is morphologically similar to the polyphyletic, cosmopolitan Leptolyngbya spp.; however, it forms an independent clade with 16S rRNA phylogeny and has a unique 16S–23S internal transcribed spacer secondary structure. To date, no cyanobacteria have been isolated and cultured from the surfaces of microplastic particles. Using a polyphasic approach combining morphological, phylogenetic and ecological traits, we describe a new genus and species Sphaerothrix gracilis. The presence of certain cyanobacteria rafting on microplastic surfaces can potentially indicate a long‐distance transport into new ecosystems with implications on toxicity and biodiversity. [ABSTRACT FROM AUTHOR]

While the immunodeficient status of NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) and NSG-related mice provides utility for numerous research models, it also results in increased susceptibility to opportunistic pathogens. Over a 9-week period, a high rate of mortality was reported in a housing room of NSG and NSG-related mice. Diagnostics were performed to determine the underlying etiopathogenesis. Mice submitted for evaluation included those found deceased (n = 2), cage mates of deceased mice with or without diarrhea (n = 17), and moribund mice (n = 8). Grossly, mice exhibited small intestinal and cecal dilation with abundant gas and/or digesta (n = 18), serosal hemorrhage and congestion (n = 6), or were grossly normal (n = 3). Histologically, there was erosive to ulcerative enterocolitis (n = 7) of the distal small and large intestine or widespread individual epithelial cell death with luminal sloughing (n = 13) and varying degrees of submucosal edema and mucosal hyperplasia. Cecal dysbiosis, a reduction in typical filamentous bacteria coupled with overgrowth of bacterial rods, was identified in 18 of 24 (75%) mice. Clostridium spp. and Paeniclostridium sordellii were identified in 13 of 23 (57%) and 7 of 23 (30%) mice, respectively. Clostridium perfringens (7 of 23, 30%) was isolated most frequently. Toxinotyping of C. perfringens positive mice (n = 2) identified C. perfringens type A. Luminal immunoreactivity to several clostridial species was identified within lesioned small intestine by immunohistochemistry. Clinicopathologic findings were thus associated with overgrowth of various clostridial species, though direct causality could not be ascribed. A diet shift preceding the mortality event may have contributed to loss of intestinal homeostasis. [ABSTRACT FROM AUTHOR]

Type IV pili are filamentous appendages found in most bacteria and archaea, where they can support functions such as surface adhesion, DNA uptake, aggregation, and motility. In most bacteria, PilT-family ATPases disassemble adhesion pili, causing them to rapidly retract and produce twitching motility, important for surface colonization. As archaea do not possess PilT homologs, it was thought that archaeal pili cannot retract and that archaea do not exhibit twitching motility. Here, we use live-cell imaging, automated cell tracking, fluorescence imaging, and genetic manipulation to show that the hyperthermophilic archaeon Sulfolobus acidocaldarius exhibits twitching motility, driven by retractable adhesion (Aap) pili, under physiologically relevant conditions (75 °C, pH 2). Aap pili are thus capable of retraction in the absence of a PilT homolog, suggesting that the ancestral type IV pili in the last universal common ancestor (LUCA) were capable of retraction. Bacteria use filamentous appendages known as type IV pili for various functions, including twitching motility on surfaces. It was thought that archaeal pili cannot retract and that archaea do not exhibit twitching motility. However, Charles-Orszag et al. demonstrate that the model archaeon Sulfolobus acidocaldarius exhibits twitching motility, driven by retractable adhesion pili. [ABSTRACT FROM AUTHOR]

Filamentous cyanobacteria are one of the oldest and today still most abundant lifeforms on earth, with manifold implications in ecology and economics. Their flexible filaments, often several hundred cells long, exhibit gliding motility in contact with solid surfaces. The underlying force generating mechanism is not yet understood. Here, we demonstrate that propulsion forces and friction coefficients are strongly coupled in the gliding motility of filamentous cyanobacteria. We directly measure their bending moduli using micropipette force sensors, and quantify propulsion and friction forces by analyzing their self-buckling behavior, complemented with analytical theory and simulations. The results indicate that slime extrusion unlikely generates the gliding forces, but support adhesion-based hypotheses, similar to the better-studied single-celled myxobacteria. The critical self-buckling lengths align well with the peaks of natural length distributions, indicating the importance of self-buckling for the organization of their collective in natural and artificial settings. [ABSTRACT FROM AUTHOR]

This is the first report on abundance and diversity of cultured filamentous fungi and on abundance of yeasts in the sediments of the Laptev and East Siberian seas. A total of 63 isolates of filamentous fungi belonging to 32 morphotypes were obtained from 35 sediment samples. Most morphotypes were represented by single colonies in individual samples; the most frequently occurring species were Pseudeurotiumzonatum, Pseudogymnoascuspannorum, and Cordycepsfarinosа. In both seas, the abundance of filamentous fungi (up to 12 colonies per 1 g wet sediment) was lower than that of yeasts (up to 68 colonies per 1 g wet sediment). In the Laptev Sea, yeast abundance was low at all stations, while in the East Siberian Sea higher values were found at near-estuarine stations. [ABSTRACT FROM AUTHOR]

Introduction: Microplastics (MPs), identified as emerging contaminants, have been detected across diverse environmental media. Their enduring presence and small size facilitate the adsorption of organic pollutants and heavy metals, leading to combined pollution effects. MPs also accumulate in the food chain thus pose risks to animals, plants, and human health, garnering significant scholarly attention in recent years. Aerobic granular sludge (AGS) technology emerges as an innovative approach to wastewater treatment. However, the impacts of MPs on the operational efficiency and microbial characteristics of AGS systems has been insufficiently explored. Methods: This study investigated the effects of varying concentration (10, 50, and 100 mg/L) of biodegradable MPs (Polylactic Acid, PLA) and nonbiodegradable MPs (Polyethylene Terephthalate, PET) on the properties of AGS and explored the underlying mechanisms. Results and discussions: It was discovered that low and medium concentration of MPs (10 and 50 mg/L) showed no significant effects on COD removal by AGS, but high concentration (100 mg/L) of MPs markedly diminished the ability to remove COD of AGS, by blocking most of the nutrient transport channels of AGS. However, both PLA and PE promoted the nitrogen and phosphorus removal ability of AGS, and significantly increased the removal efficiency of total inorganic nitrogen (TIN) and total phosphorus (TP) at stages II and III (P < 0.05). High concentration of MPs inhibited the growth of sludge. PET noticeably deteriorate the sedimentation performance of AGS, while 50 mg/L PLA proved to be beneficial to sludge sedimentation at stage II. The addition of MPs promoted the abundance of Candidatus_Competibacter and Acinetobacter in AGS, thereby promoting the phosphorus removal capacity of AGS. Both 50 mg/L PET and 100 mg/L PLA caused large amount of white Thiothrix filamentous bacteria forming on the surface of AGS, leading to deterioration of the sludge settling performance and affecting the normal operation of the reactor. Comparing with PET, AGS proved to be more resistant to PLA, so more attention should be paid to the effect of non-biodegradable MPs on AGS in the future. [ABSTRACT FROM AUTHOR]

Cellulosimicrobium species (formerly known as Oerskovia) are Gram-positive filamentous bacteria in the family Promicromonosporaceae and are more commonly found in sewage and soil. The present study aimed to identify all the published cases of Cellulosimicrobium species infections in the literature, describe the epidemiological, clinical, and microbiological characteristics, and provide data regarding its antimicrobial resistance, treatment, and outcomes. A narrative review was performed based on a PubMed and Scopus database search. In total, 38 studies provided data on 40 patients with infections by these species. The median age of patients was 52.5 years, and 55% were male. The most common infection types were bacteremia, infective endocarditis (IE), osteoarticular infections, peritoneal dialysis-associated peritonitis, and endophthalmitis. Antimicrobial resistance to vancomycin and the combination of trimethoprim and sulfamethoxazole was minimal, and vancomycin was the most commonly used antimicrobial for treating these infections. Overall mortality was minimal for all infections, except for bacteremia and IE, which carried high mortality rates. [ABSTRACT FROM AUTHOR]

The ongoing global warming is likely to cause changes in the growth and species composition of aquatic epilithic communities. To be able to predict such changes, a field study was conducted in the cooling water discharge area of a nuclear power plant that releases brackish cooling water to the Baltic Sea at Forsmark. The power plant creates a temperature gradient in the field from 0 to ca. 8 °C above normal temperature. The project was concentrated on the growth and distribution of four dominant taxa in the upper littoral zone: the colonial cyanobacterium Rivularia atra and the three filamentous macroalgae Cladophora glomerata (green), Ulva spp. (green) and Ceramium tenuicorne (red). Overall, Cladophora increased strongly, Ulva spp. increased slightly and Ceramium decreased strongly in abundance with higher water temperature. For Rivularia these results were corroborated in a laboratory experiment and studied in more detail. The colonies collected at 18 °C in the field were experimentally exposed to 18 °C, 22 °C and 26 °C in climate chambers for 50 days. Growth rates were highest in 18 °C and lowest in 22 and 26 °C. Addition of extra stress in the form of a heavy metal (copper) decreased growth in all temperatures, but highest growth rates still occurred in 18 °C. Rivularia was less stressed in 18 °C than in 22 °C and 26 °C, which was shown by, higher chlorophyll a (chla) concentration, higher photosynthetic performance (measured as electron transport rate by pulse amplitude modulated fluorescence), lower zeaxanthin concentration and a lower carotenoids:chla ratio. [ABSTRACT FROM AUTHOR]

The VITEK MS PRIME (bioMérieux, Marcy-l'Étoile, France), a newly developed matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system, alongside the VITEK PICKME pen (PICKME), offers easy sample preparation for bacteria and yeasts. The VITEK MS PRIME also offers two software platforms for filamentous fungi: the IVD database and the RUO database. Our study evaluated its identification agreement on 320 clinical isolates of bacteria and yeasts, comparing PICKME and traditional wooden toothpick sampling techniques against MicroIDSys Elite (ASTA) results. Additionally, we assessed the IVD (v3.2) and SARAMIS (v4.16) RUO databases on 289 filamentous fungi against molecular sequencing. The concordance rates for species-level identification of bacteria and yeasts were about 89.4% (286/320) between the PICKME and wooden toothpick, and about 83.4–85.3% between the VITEK MS PRIME and ASTA MicroIDSys Elite. Retesting with PICKME improved concordance to 91.9%. For filamentous fungi, species-level identification reached 71.3% with the IVD database and 85.8% with RUO, which significantly enhanced basidiomycetes' identification from 35.3% to 100%. Some strains in the IVD database, like Aspergillus versicolor, Exophiala xenobiotica, and Nannizzia gypsea, failed to be identified. The VITEK MS PRIME with PICKME offers reliable and efficient microorganism identification. For filamentous fungi, combined use of the RUO database can be beneficial, especially for basidiomycetes. [ABSTRACT FROM AUTHOR]

A novel endophytic actinomycete, strain MEP2-6T, was isolated from scab tissues of potato tubers collected from Mae Fag Mai Sub-district, San Sai District, Chiang Mai Province, Thailand. Strain MEP2-6T is a gram-positive filamentous bacteria characterized by meso-diaminopimelic acid in cell wall peptidoglycan and arabinose, galactose, glucose, and ribose in whole-cell hydrolysates. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and hydroxy-phosphatidylethanolamine were the major phospholipids, of which MK-9(H6) was the predominant menaquinone, whereas iso-C16:0 and iso-C15:0 were the major cellular fatty acids. The genome of the strain was 10,277,369 bp in size with a G + C content of 71.7%. The 16S rRNA gene phylogenetic and core phylogenomic analyses revealed that strain MEP2-6T was closely related to Amycolatopsis lexingtonensis NRRL B-24131T (99.4%), A. pretoriensis DSM 44654T (99.3%), and A. eburnea GLM-1T (98.9%). Notably, strain MEP2-6T displayed 91.7%, 91.8%, and 87% ANIb and 49%, 48.8%, and 35.4% dDDH to A. lexingtonensis DSM 44653T (=NRRL B-24131T), A. eburnea GLM-1T, and A. pretoriensis DSM 44654T, respectively. Based on phenotypic, chemotaxonomic, and genomic data, strain MEP2-6T could be officially assigned to a novel species within the genus Amycolatopsis, for which the name Amycolatopsis solani sp. nov. has been proposed. The type of strain is MEP2-6T (=JCM 36309T = TBRC 17632T = NBRC 116395T). Amycolatopsis solani MEP2-6T was strongly proven to be a non-phytopathogen of potato scab disease because stunting of seedlings and necrotic lesions on potato tuber slices were not observed, and there were no core biosynthetic genes associated with the BGCs of phytotoxin-inducing scab lesions. Furthermore, comparative genomics can provide a better understanding of the genetic mechanisms that enable A. solani MEP2-6T to adapt to the plant endosphere. Importantly, the strain smBGCs accommodated 33 smBGCs encoded for several bioactive compounds, which could be beneficially applied in the fields of agriculture and medicine. Consequently, strain MEP2-6T is a promising candidate as a novel biocontrol agent and antibiotic producer. [ABSTRACT FROM AUTHOR]