214 results on '"dos Santos JN"'
Search Results
2. Intraoral lipomas: a study of 26 cases in a Brazilian population.
- Author
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de Freitas MA, Freitas VS, de Lima AAS, Pereira FB Jr., and dos Santos JN
- Abstract
Objective: To analyze the clinical and histopathologic features of all cases diagnosed as lipomas in the oral cavity in a Brazilian population. Method and Materials: From 2002 to 2006, 26 cases of intraoral lipoma were studied. Clinical features obtained from biopsy records and microscopic slides were reviewed according to the World Health Organization and other cases previously described. Results: Mean age was 54.6 years (range, 29 to 91 years) with predilection for women. The buccal mucosa was the most common location (n = 9), followed by the tongue (n = 7), lower lip (n = 4), floor of the mouth (n = 3), retromolar area (n = 2), and buccal sulcus (n = 1). Histologically, the lesions were defined as classic lipomas (n = 15), fibrolipomas (n = 7), intramuscular lipoma (n = 2), spindle-cell lipoma (n = 1), and sialolipoma (n = 1). Follow-up information was available in 20 cases. No case recurred during a 6- to 12-month period. Conclusion: The benign nature of intraoral lipomas is supported by its bland histopathologic appearance and the absence of recurrences following complete local excision. [ABSTRACT FROM AUTHOR]
- Published
- 2009
3. Adenomatoid odontogenic tumor: an unusual case exhibiting cribriform aspect.
- Author
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dos Santos JN, de Lima FO, Romério P, and Souza VF
- Abstract
The occurrence of areas exhibiting cribriform morphology in odontogenic tumors is not a very frequent finding. This report describes the case of a 22-year-old man who presented with swelling in the left anterior maxilla. An excisional biopsy was performed under local anesthesia. Histopathologically, the lesion consisted of a cystic wall exhibiting an odontogenic epithelium lining composed of oval, angular, and elongated cells forming a cribriform pattern. No recurrence has been detected after 2 years. [ABSTRACT FROM AUTHOR]
- Published
- 2008
4. Human intraocular filariasis caused by Dirofilaria sp. nematode, Brazil
- Author
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Otranto, D, Diniz, D, Dantas Torres, F, Casiraghi, M, De Almeida, I, De Almeida, L, Dos Santos, J, Furtado, A, De Almeida Sobrinho, E, Bain, O, Diniz, DG, De Almeida, IN, De Almeida, LN, Dos Santos, JN, Furtado, AP, De Almeida Sobrinho, EF, Bain, O., CASIRAGHI, MAURIZIO, Otranto, D, Diniz, D, Dantas Torres, F, Casiraghi, M, De Almeida, I, De Almeida, L, Dos Santos, J, Furtado, A, De Almeida Sobrinho, E, Bain, O, Diniz, DG, De Almeida, IN, De Almeida, LN, Dos Santos, JN, Furtado, AP, De Almeida Sobrinho, EF, Bain, O., and CASIRAGHI, MAURIZIO
- Abstract
A case of human intraocular dirofilariasis is reported from northern Brazil. The nematode was morphologically and phylogenetically related to Dirofilaria immitis but distinct from reference sequences, including those of D. immitis infesting dogs in the same area. A zoonotic Dirofilaria species infesting wild mammals in Brazil and its implications are discussed
- Published
- 2011
5. Beta-catenin and surviving expression in keratocystic odontogenic tumor (KCOT). A comparative immunohistochemical study in primary, recurrent and nevoid basal cell carcinoma syndrome (NBCCS)-associated lesions
- Author
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Leonardi, R., Matthews, J. B., Loreto, C., Musumeci, G., giuseppina campisi, Lo Muzio, L., Dos Santos, J. N., Pastorino, L., Bufo, P., Pannone, G., Leonardi, R, Matthews, JB, Loreto, C, Musumeci, G, Campisi, G, Lo Muzio, L, dos Santos, JN, Pastorino, L, Bufo, P, and Pannone, G
- Subjects
Adult ,Male ,Histology ,Adolescent ,Survivin ,6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología [CDU] ,Settore MED/50 - Scienze Tecniche Mediche Applicate ,Apoptosis ,Odontogenic Tumors ,ß-catenin ,NBCCS ,Inhibitor of Apoptosis Proteins ,Settore MED/29 - Chirurgia Maxillofacciale ,Young Adult ,Recurrence ,Settore MED/28 - Malattie Odontostomatologiche ,Humans ,beta Catenin ,Cell Cycle ,NBCCS, ß-catenin, Survivin, KCOT ,Basal Cell Nevus Syndrome ,Middle Aged ,Immunohistochemistry ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,KCOT ,2734 ,Keratins ,Female - Abstract
Aim: To determine the epithelial expression of ß-catenin and survivin in sporadic (primary, and recurrent) and nevoid basal cell carcinoma syndrome (NBCCS) keratocystic odontogenic tumour (KCOT) in order to assess activation of the ß-catenin pathway and evidence of apoptotic inhibition, processes that may contribute to the known differences in their biological behaviour. Materials and Methods: Sections from 40 cases of KCOT (19 sporadic/primary; 9 sporadic/recurrent and 12 NBCCS-associated) were immunohistochemically stained for ß-catenin and survivin. The extent and intensity of immunoreactivity within the lining epithelium was assessed, using semi-quantitative scales, independently by two pathologists who were blinded to the clinical-pathological data. Data were analysed using Kruskal-Wallis test and, for pair-wise comparisons, Mann-Whitney test with Bonferroni correction. Results: All cystic epithelial linings stained for ß- catenin and survivin but there were differences in the pattern and intensity of staining among KCOT types. Sporadic primary KCOT showed weaker staining for ß- catenin (P=0.0003) and survivin (P
- Published
- 2013
6. The Impact of Heavy Metal Contamination in Humans and Periodontitis: A Scoping Review.
- Author
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Coelho T, Cardoso CN, Carvalho MJF, Dos Santos JN, Ribeiro DA, and Cury PR
- Abstract
Periodontal disease, one of the most prevalent diseases worldwide, is a chronic inflammatory disease caused by dysbiotic dental biofilms that trigger the host's immune response. Periodontitis is a type of periodontal disease characterized by the destruction of tissues that support the teeth. The disease is influenced by various systemic and environmental risk factors. As heavy metals have been associated with the development of chronic inflammatory diseases, the present scoping review aimed to determine the coverage of the literature on whether human contamination by heavy metals affects periodontitis, as well as their mechanisms of action. Eight studies were selected, and two reviewers evaluated them. Most studies were cross-sectional studies involving humans and one study was performed on rats. Our review revealed a significant correlation between periodontitis and bioaccumulation of lead and cadmium. Oxidative stress generated by trace metals, characterized by elevated levels of reactive oxygen species, causes tissue damage through lipid peroxidation, enzymatic oxidation, and stimulation of proinflammatory cytokines. In conclusion, heavy metals contamination may be a risk factor for the development of periodontitis. Oxidative stress factors seem to increase the extent of the inflammatory response., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2024
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7. Susceptibility evaluation of novel beta-lactam/beta-lactamase inhibitor combinations against carbapenem-resistant Klebsiella pneumoniae from bloodstream infections in hospitalized patients in Brazil.
- Author
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Wilhelm CM, Antochevis LC, Magagnin CM, Arns B, Vieceli T, Pereira DC, Lutz L, de Souza ÂC, Dos Santos JN, Guerra RR, Medeiros GS, Santoro L, Falci DR, Rigatto MH, Barth AL, Martins AF, and Zavascki AP
- Subjects
- Humans, Brazil, Bacterial Proteins genetics, Meropenem pharmacology, Imipenem pharmacology, Bacteremia microbiology, Boronic Acids pharmacology, Heterocyclic Compounds, 1-Ring, Microbial Sensitivity Tests, Klebsiella pneumoniae drug effects, Klebsiella pneumoniae genetics, Klebsiella pneumoniae isolation & purification, beta-Lactamase Inhibitors pharmacology, Klebsiella Infections microbiology, Azabicyclo Compounds pharmacology, Ceftazidime pharmacology, Drug Combinations, beta-Lactamases genetics, Anti-Bacterial Agents pharmacology, Carbapenem-Resistant Enterobacteriaceae drug effects, Carbapenem-Resistant Enterobacteriaceae genetics, Carbapenem-Resistant Enterobacteriaceae isolation & purification
- Abstract
Introduction: Novel beta-lactam/beta-lactamase inhibitor (BIBLI) combinations are commercially available and have been used for treating carbapenem-resistant Klebsiella pneumoniae (CRKP) infections. Continuous surveillance of susceptibility profiles and resistance mechanism identification are necessary to monitor the evolution of resistance within these agents., Objective: The purpose of this study was to evaluate the susceptibility rates of ceftazidime/avibactam, imipenem/relebactam and meropenem/vaborbactam in CRKP isolated from patients with bloodstream infections who underwent screening for a randomized clinical trial in Brazil., Methods: Minimum inhibitory concentrations (MICs) were determined for meropenem, ceftazidime/avibactam, imipenem/relebactam and meropenem/vaborbactam using the gradient diffusion strip method. Carbapenemase genes were detected by multiplex real-time polymerase chain reaction. Klebsiella pneumoniae carbapenemase (KPC)-producing isolates showing resistance to any BLBLI and New Delhi Metallo-beta-lactamase (NDM)-producing isolates with susceptibility to any BLBLI isolates were further submitted for whole-genome sequencing., Results: From a total of 69 CRKP isolates, 39 were positive for bla
KPC , 19 for blaNDM and 11 for blaKPC and blaNDM . KPC-producing isolates demonstrated susceptibility rates above 94 % for all BLBLIs. Two isolates with resistance to meropenem/vaborbactam demonstrated a Gly and Asp duplication at the porin OmpK36 as well as a truncated OmpK35. All NDM-producing isolates, including KPC and NDM coproducers, demonstrated susceptibility rates to ceftazidime/avibactam, imipenem/relebactam and meropenem/vaborbactam of 0 %, 9.1-21.1 % and 9.1-26.3 %, respectively. Five NDM-producing isolates that presented susceptibility to BLBLIs also had porin alterations CONCLUSIONS: This study showed that, although high susceptibility rates to BLBLIs were found, KPC-2 isolates were able to demonstrate resistance probably as a result of porin mutations. Additionally, NDM-1 isolates showed susceptibility to BLBLIs in vitro., Competing Interests: Competing interests A.P.Z. is a research fellow of the National Council for Scientific and Technological Development (CNPq/Brazil). The authors have no relevant financial or non-financial interests to disclose., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2024
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8. Pleomorphic adenoma and carcinoma ex-pleomorphic adenoma tumorigenesis: A proteomic analysis.
- Author
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Zanella VG, Costa SFDS, Schuch LF, Pilar EFS, Paes Leme AF, Dos Santos JN, Khurram SA, Elalawy F, Bingle L, Nunes FD, Fonseca FP, Vargas PA, Martins MD, and Wagner VP
- Abstract
Objectives: To conduct a comprehensive proteomic analysis of normal salivary gland tissue, pleomorphic adenoma (PA), and carcinoma ex-pleomorphic adenoma (CXPA), and validate the proteomic findings using immunohistochemistry., Methods: Six normal salivary gland tissues, seven PA and seven CXPA samples underwent laser microdissection followed by liquid chromatography coupled to mass spectrometry. Protein identification and quantification were performed using MaxQuant software. Statistical analysis and functional enrichment were conducted using the Perseus platform and STRING tool, respectively. Immunohistochemistry was used for validation., Results: Comparative proteomic analysis revealed 2680 proteins across the three tissue types, with 799 significantly altered between groups. Translocation protein SEC63 homolog, Annexin A6 and Biglycan were up-regulated in CXPA compared to PA. Decorin was markedly up-regulated in both PA and CXPA compared to normal salivary gland (log
2 fold changes of 7.58 and 7.38, respectively). Validation confirmed elevated levels of Biglycan and Decorin in the extracellular matrix of CXPA compared to PA., Conclusions: Proteomic analysis identified differential protein expression patterns associated with malignant transformation of PA into CXPA. Findings indicate a crucial role for extracellular matrix proteins, specifically Biglycan and Decorin, in the tumorigenic progression of PA and CXPA., (© 2024 The Author(s). Oral Diseases published by John Wiley & Sons Ltd.)- Published
- 2024
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9. Acanthoatractis xinguensis n. gen., n. sp. (Nematoda: Cosmocercoidea: Atractidae) parasite of yellow-spotted Amazon river turtle, Podocnemis unifilis Troschel (Testudines: Podocnemididae) in Brazilian Amazon.
- Author
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Jesus RF, Nandyara B, Dos Santos JN, and Melo FTV
- Abstract
Nematodes collected from the stomach of the yellow-spotted turtle Podocnemis unifilis Troschel, 1848 (Testudinidae) in the Brazilian state of Pará are assigned to a new genus, allocated to the family Atractidae (Cosmocerdoidea). Acanthoatractis n. gen. differs from all other genera of Atractidae based on the combination of the following morphological characters: cephalic extremity surrounded by eight bifurcated, wrench-shaped sclerotized structures arranged in a circle; oral opening encircled by two sclerotized pieces with pointed ends and a median portion with a pair of hooks; in males the larger (left) spicule is narrower in the middle third and the gubernaculum is absent. The type species, Acanthoatractis xinguensis n. gen., n. sp., has nine pairs of caudal papillae and a single papilla anterior to the cloacal lip. The new species is the seventh record of an atractid genus parasitizing P. unifilis ., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)
- Published
- 2024
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10. Globe-shaped central incisors in a patient with otodental syndrome.
- Author
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Souza DAS, Duplat CB, Oliveira RB, Neves FS, Machado AW, and Dos Santos JN
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- Humans, Male, Child, Tooth, Supernumerary complications, Tooth, Supernumerary diagnostic imaging, Tooth, Supernumerary surgery, Tooth Abnormalities diagnosis, Diagnosis, Differential, Abnormalities, Multiple, Bone Diseases, Developmental, Intellectual Disability, Facies, Incisor abnormalities
- Abstract
Hearing impairments and dental anomalies are found in many genetic syndromes. Otodental syndrome is a rare combination of hearing loss and the presence of a pathognomonic dental phenotype known as globodontia, in which the tooth exhibits an abnormal globe shape. There is no histologic evidence of structural anomalies in the enamel, dentin, or pulp. This report describes the case of a 12-year-old boy who had hearing loss and 2 supernumerary globe-shaped teeth in the sites of the permanent maxillary central incisors. The diagnosis of otodental syndrome was established based on the clinical, radiographic, and histologic features, but other conditions, including dens evaginatus, talon cusp, dens invaginatus, and compound odontoma, should be included in the differential diagnosis. Dental treatment consisted of the extraction of both anomalous teeth, allowing spontaneous eruption of the impacted permanent central incisors. Early diagnosis of otodental syndrome permits a multidisciplinary approach to prevent other pathologic conditions, reduce functional damage, and avoid social problems., Competing Interests: No conflicts of interest reported.
- Published
- 2024
11. Chronic Sclerosing Sialadenitis of the Submandibular Gland and its Histopathological Spectrum in the IgG4-Related Disease: a Series of 17 Cases.
- Author
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Muniz VRVM, Altemani A, Freitas VS, Pires BC, de Santana DA, Couto LA, Cangussu MCT, Gomez RS, de Souza SCOM, Vargas PA, Cury PR, de Araújo IB, Chaves RRM, Fonseca FP, and Dos Santos JN
- Subjects
- Humans, Male, Female, Middle Aged, Adult, Aged, Sclerosis pathology, Chronic Disease, Submandibular Gland pathology, Immunohistochemistry, Sialadenitis pathology, Immunoglobulin G4-Related Disease pathology
- Abstract
Purpose: This study aimed to characterize the histopathological immunohistochemical features of chronic sclerosing sialadenitis, emphasizing the IgG4-related disease., Methods: Seventeen cases of chronic sclerosing sialoadenitis were examined for histopathological aspects, (inflammation, fibrosis, glandular parenchyma, and lymphoid follicles) and immunohistochemistry (BCL2, CD3, CD20, CD34, CD163, p63, cyclin D1, mast cell, SMA, S100A4, IgG, and IgG4) which were scored. IgG4-related disease features were investigated. Demographic and clinical data were also collected., Results: Males predominated (10:7), with an average lesion size of 3.9 cm. Common histopathological findings included reduced acinar parenchyma, lymphoid follicle formation, and ductular proliferation. CD3-positive T lymphocytes and CD34- and SMA-positive stromal fibroblasts were abundant. Nine cases (53%) showed sialoliths and three cases met the criteria for IgG4-related disease., Conclusion: CSS of the submandibular gland represents a reactive pattern rather than IgG4-RD as only 3 cases seemed to be related to IgG4-RD. The immunohistochemical profile revealed an abundant population of CD3-positive T lymphocytes, as opposed to regulatory proteins such as cyclin D1, demonstrating that populations of CD34- and SMA-positive stromal fibroblasts contribute to the fibrosis characteristic of CSS. In addition, our results provide a comprehensive insight into the study of CSS and its relationship with IgG4-RD., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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12. Cholesterol depletion induces mesenchymal properties in oral squamous cell carcinoma cell line.
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do Nascimento RB, Cerqueira PSG, Silva JC, Fontes EK, Dos Santos EA, Dos Santos JN, Nunes FD, Rodrigues MFSD, Paiva KBS, and Xavier FCA
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- Humans, Squamous Cell Carcinoma of Head and Neck, Cell Line, Tumor, Cell Proliferation, Cholesterol, Epithelial-Mesenchymal Transition genetics, Cell Movement, Carcinoma, Squamous Cell pathology, Mouth Neoplasms pathology, Head and Neck Neoplasms
- Abstract
Background: Cholesterol in cell membranes is crucial for cell signaling, adhesion, and migration. Membranes feature cholesterol-rich caveolae with caveolin proteins, playing roles in epithelial-mesenchymal transition and cancer progression. Despite elevated cholesterol levels in tumors, its precise function and the effects of its depletion in oral squamous cell carcinoma remain unclear. The aim of this study was to evaluate the influence of cholesterol depletion in oral squamous cell carcinoma cell line and epithelial-mesenchymal transition process., Methods: Cholesterol depletion was induced on SCC-9 cells by methyl-β-cyclodextrin and cell viability, proliferation, apoptosis, and colony formation capacities were evaluated. Gene and protein expressions were evaluated by reverse transcription polymerase chain reaction (RT-qPCR) and Western Blot, respectively, and cell sublocalization was assessed by immunofluorescence., Results: Cholesterol depletion resulted in alteration of oral squamous cell carcinoma cell morphology at different concentrations of methyl-β-cyclodextrin, as well as decreased cell proliferation and viability rates. Analysis of CAV1 transcript expression revealed increased gene expression in the treated SCC-9 during the 24 h period, at different concentrations of methyl-β-cyclodextrin: 5 , 7.5, 10, and 15 mM, in relation to parental SCC-9. CAV1 protein expression was increased, with subsequent dose-dependent decrease. A statistically significant difference was observed in samples treated with 5 mM of methyl-β-cyclodextrin (p = 0.02, Kruskal-Wallis test). The immunofluorescence assay showed lower cytoplasmic and membrane labeling intensity in the treated samples for CAV1., Conclusion: These findings indicate the modulation of cholesterol as a possible mechanism underlying the regulation of these molecules and activation of epithelial-mesenchymal transition in oral squamous cell carcinoma., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2024
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13. E-CADERIN, N-CADERIN, SLUG, SNAIL, and TWIST contribute to epithelial-mesenchymal transition in salivary gland tumors.
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de Santana DA, Braga PR, Camillo-Coutinho CM, Freitas VS, Cury PR, Ribeiro DA, de Araújo IB, de Aquino Xavier FC, and Dos Santos JN
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- Humans, Adult, Snail Family Transcription Factors, Nuclear Proteins genetics, Cadherins genetics, Epithelial-Mesenchymal Transition genetics, Biomarkers, Tumor, Twist-Related Protein 1 genetics, Salivary Gland Neoplasms pathology, Carcinoma, Adenoid Cystic pathology, Carcinoma, Mucoepidermoid pathology, Adenoma, Pleomorphic pathology
- Abstract
Background: Transcription factors are important in the epithelial-mesenchymal transition process and are possibly related to the development of a more invasive tumor phenotype. Thus, the objective of this study was to analyze the expression and identify the localization of cellular markers related to the epithelial-mesenchymal transition process in salivary gland tumors., Study Design: The expression and localization of E-CADERIN, N-CADERIN, SLUG, SNAIL, and TWIST were evaluated, using immunohistochemistry, in 48 salivary gland tumors, being 17 pleomorphic adenomas (PA), 14 adenoid cystic carcinomas (ACC), and 17 mucoepidermoid carcinomas (MEC). these proteins were compared to clinical and histopathologic parameters. normal gland tissues were included for immunohistochemical comparisons., Results: ACC and MEC cases showed higher expression of SNAIL compared to PA. MEC showed high expression of SLUG and TWIST. Low expression of N-CADHERIN, SNAIL, and TWIST in ACC was frequent in T3 and T4. High expression of TWIST in MEC was more frequent at age ≥ 40 years A positive correlation was only observed between N-cadherin/SNAIL in ACC, between SNAIL/TWIST in MEC, and between SLUG/TWIST in PA., Conclusion: This study provided insight into EMT-related proteins (E-cadherin, N-cadherin, SNAIL, SLUG, and TWIST) and their contribution to the maintenance of morphogenesis and the development of the salivary gland tumors and showed a positive correlation among N-CADHERIN/SNAIL in ACC and SNAIL/TWIST in MEC., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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14. Could fluoride be considered a genotoxic chemical agent in vivo? A systematic review with meta-analysis.
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Drummond GWB, Takeshita WM, de Castro GM, Dos Santos JN, Cury PR, Renno ACM, and Ribeiro DA
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- Humans, DNA Damage drug effects, Animals, Mutagenicity Tests, Fluorides toxicity, Mutagens toxicity
- Abstract
The goal of this study was to perform systematic review (SR) to investigate the scientific literature regarding the genotoxicity effects of fluoride exposure (FE). The search of databases used for this study was PubMed/Medline, SCOPUS and Web of Science. The quality of included studies was assessed using the EPHPP (Effective Public Health Practice Project). A total of 20 potentially relevant studies were selected for evaluating the genotoxicity induced by fluoride. Few studies have revealed that FE induces genotoxicity. A total of 14 studies demonstrated negative results whereas 6 studies did not. After reviewing the twenty studies, 1 was classified as weak, 10 were considered moderate and 9 were considered strong, according to the EPHPP. Taken together, it has been established that genotoxicity of fluoride is limited.
- Published
- 2024
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15. Influence of keratinized mucosa on peri-implant diseases: a retrospective cohort study in humans.
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Oliveira CAB, Pereira VL, Dos Santos JN, Araujo NS, and Cury PR
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- Male, Female, Humans, Retrospective Studies, Mouth Mucosa, Peri-Implantitis etiology, Dental Implants, Mucositis
- Abstract
Objective: The present study aimed to assess the relationship between keratinized mucosa width and peri-implant diseases, namely peri-implant mucositis and peri-implantitis., Materials and Methods: Ninety-one dental implants in function for ≥ 6 months from 40 partially or completely edentulous non-smoker subjects (24 females and 16 males) were evaluated clinically and radiographically. The width of keratinized mucosa, probing depth, plaque index, bleeding on probing, and marginal bone levels were assessed. Keratinized mucosa width was categorized as ≥ 2 mm or < 2 mm., Results: There was no statistically significant association between keratinized buccal mucosa width and peri-implant mucositis or peri-implantitis (p ≥ 0.37). In the regression analysis, peri-implantitis was associated with longer implant function time (RR: 2.55, 95% CI: 1.25-11.81, p = 0.02) and implants in the maxilla (RR: 3.15, 95% CI: 1.61-14.93, p = 0.003). Mucositis was not associated with any of the factors analyzed., Conclusion: In conclusion, in the present sample, keratinized buccal mucosa width was not associated with peri-implant diseases, suggesting that a band of keratinized mucosa may not be necessary to maintain peri-implant health. Prospective studies are required to better understand its role in the maintenance of peri-implant health., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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- 2024
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16. Oral carcinoma in situ in a middle-aged woman with systemic sclerosis: Report of a rare case and literature review.
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de Sena ACVP, de Arruda JAA, Felix FA, Tavares TS, Abreu LG, Dos Santos JN, and Silva TA
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- Middle Aged, Humans, Female, Mouth Mucosa pathology, Scleroderma, Systemic complications, Scleroderma, Systemic pathology, Mouth Neoplasms complications, Mouth Neoplasms diagnosis, Mouth Neoplasms pathology, Oropharyngeal Neoplasms pathology, Carcinoma in Situ complications, Carcinoma in Situ surgery, Carcinoma in Situ pathology
- Abstract
Systemic sclerosis (SSc) is a rare multisystem rheumatic autoimmune disease involving the skin, connective tissue, and internal organs. Individuals with SSc are at increased risk of cancer. We herein contribute by reporting a case of carcinoma in situ affecting the lower lip and labial mucosa of a 56-year-old Brazilian female patient, which apparently represents the first case reported in Latin America. Surgical resection of the lesion was performed. After a 2-year follow-up, the patient has shown no evidence of recurrence. According to a literature review in PubMed, Web of Science, Scopus, and Embase databases on SSc-related oral and oropharyngeal cancer, 11 cases have been documented hitherto. Reports of oral carcinoma in individuals with SSc are rare. Clinicians should conduct regular examinations of the oral mucosa of these individuals to permit an early diagnosis, as done in the present case., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2024
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17. DNA content and clinicopathological features aid in distinguishing ameloblastic carcinoma from ameloblastoma.
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Penafort PVM, Rocha AC, Mariano FV, Dos Santos JN, Oliveira MC, Vargas PA, and Sperandio M
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- Humans, Ki-67 Antigen genetics, Ploidies, DNA, Ameloblastoma diagnosis, Ameloblastoma genetics, Ameloblastoma pathology, Odontogenic Tumors genetics, Carcinoma pathology
- Abstract
Background: Ameloblastoma and ameloblastic carcinoma are epithelial odontogenic tumors that can be morphologically similar. In the present study, we evaluated the DNA content and Ki-67 index in the two tumors., Methods: The paraffin blocks of the tumors were selected to obtain sections for the immunohistochemical reactions and preparation of the cell suspension for acquisition in a flow cytometer. The Random Forest package of the R software was used to verify the contribution of each variable to classify lesions into ameloblastoma or ameloblastic carcinoma., Results: Thirty-two ameloblastoma and five ameloblastic carcinoma were included in the study. In our sample, we did not find statistically significant differences in Ki-67 labeling rates. A higher fraction of cells in 2c (G1) was correlated with the diagnosis of ameloblastoma, whereas higher rates of 5c-exceeding rate (5cER) were correlated with ameloblastic carcinoma. The Random Forest model highlighted histopathological findings and parameters of DNA ploidy study as important features for distinguishing ameloblastoma from ameloblastic carcinoma., Conclusion: Our findings suggest that the parameters of the DNA ploidy study can be ancillary tools in the classification of ameloblastoma and ameloblastic carcinoma., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2024
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18. Contribution of public oral pathology services to the diagnosis of oral and oropharyngeal cancer in Brazil.
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Louredo BVR, Curado MP, Penafort PVM, de Arruda JAA, Abreu LG, Mesquita RA, Pinto-Júnior DDS, Abrahão AC, Andrade BAB, Agostini M, Moraes RM, Anbinder AL, Dourado PHS, Santos TCRBD, Pires FR, Bordignon NCT, Gondak RO, DE Oliveira MG, Carrard VC, Martins MD, Sousa-Neto SS, Arantes DAC, Mendonça EF, Cieslak-Sanches SR, Antunes DM, Amaral-Silva GKD, Manieri PR, Ramalho LMP, Dos Santos JN, Leonel ACLDS, Perez DEDC, Verheul HCCRS, Barroso KMA, Rodrigues FLS, Gonzaga AKG, Fernandes RR, DE Souza LB, Souza LL, Pontes FSC, Pontes HAR, Silva CA, Câmara J, Libório-Kimura TN, Santos-Silva AR, Lopes MA, Almeida OP, Romañach MJ, and Vargas PA
- Subjects
- Male, Humans, Middle Aged, Brazil epidemiology, Pathology, Oral, Cross-Sectional Studies, Squamous Cell Carcinoma of Head and Neck, Oropharyngeal Neoplasms diagnosis, Oropharyngeal Neoplasms epidemiology, Head and Neck Neoplasms
- Abstract
This study aimed to evaluate the contribution of oral and maxillofacial pathology laboratories (OMPLs) in Brazilian public universities to the diagnosis of lip, oral cavity, and oropharyngeal squamous cell carcinoma (SCC). A cross-sectional study was performed using biopsy records from a consortium of sixteen public OMPLs from all regions of Brazil (North, Northeast, Central-West, Southeast, and South). Clinical and demographic data of patients diagnosed with lip, oral cavity, and oropharyngeal SCC between 2010 and 2019 were collected from the patients' histopathological records. Of the 120,010 oral and maxillofacial biopsies (2010-2019), 6.9% (8,321 cases) were diagnosed as lip (0.8%, 951 cases), oral cavity (4.9%, 5,971 cases), and oropharyngeal (1.2%, 1,399 cases) SCCs. Most cases were from Brazil's Southeast (64.5%), where six of the OMPLs analyzed are located. The predominant profile of patients with lip and oral cavity SCC was Caucasian men, with a mean age over 60 years, low schooling level, and a previous history of heavy tobacco consumption. In the oropharyngeal group, the majority were non-Caucasian men, with a mean age under 60 years, had a low education level, and were former/current tobacco and alcohol users. According to data from the Brazilian National Cancer Institute, approximately 9.9% of the total lip, oral cavity, and oropharyngeal SCCs reported over the last decade in Brazil may have been diagnosed at the OMPLs included in the current study. Therefore, this data confirms the contribution of public OMPLs with respect to the important diagnostic support they provide to the oral healthcare services extended by the Brazilian Public Health System.
- Published
- 2023
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19. Are Cytomorphogenetic Events Correlated with Oral Mucosal Lesions Induced by Crack Cocaine Use? A Systematic Review.
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Guedes Pinto T, Viana MB, Cury PR, Martins MD, Dos Santos JN, and Ribeiro DA
- Abstract
The aim of this systematic review was to answer the question of whether crack cocaine can induce cellular and molecular alterations and whether such alterations are somehow related to clinical lesions in the oral mucosa. The searches were undertaken in three electronic databases and conducted based on the PRISMA 2020 statement. Eleven studies published between 1994 and 2020 were analyzed. The quality of the included studies was assessed by two independent reviewers (TGP and DAR) through a confounder's categorization methodology, in which final ratings were attributed (strong, moderate or weak) for each study. From 11 studies included, 7 evaluated the cellular/molecular impact of the addiction in a total of 492 individuals and compared to a control (non-exposure) group (n = 472). The main tests used for cellular alteration were MN and AgNORs. Cells from crack cocaine groups exhibited increased proliferation and MN counting. Only four studies evaluated the prevalence of oral lesions. All of them showed that individuals exposed to crack cocaine presented an increased number of oral lesions. Most studies showed good quality. In conclusion, our results demonstrate that crack use may induce changes at the cellular and molecular level and also exhibit an increased number of oral lesions. However, a correlation between such changes and oral mucosa lesions still needs further investigation and elucidation through other clinical studies in humans.
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- 2023
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20. Photodynamic therapy as adjunctive treatment of single-rooted teeth in patients with grade C periodontitis: A randomized controlled clinical trial.
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Rodrigues RD, Araujo NS, Filho JMP, Vieira CLZ, Ribeiro DA, Dos Santos JN, and Cury PR
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- Humans, Photosensitizing Agents therapeutic use, Combined Modality Therapy, Root Planing methods, Dental Scaling, Photochemotherapy methods, Chronic Periodontitis drug therapy, Anti-Infective Agents therapeutic use
- Abstract
Background: The present study aimed to evaluate the adjunctive effect of an antimicrobial photodynamic therapy (aPDT) protocol on single-rooted teeth of patients with grade C periodontitis., Methods: Sixty-four single-rooted teeth (14 patients) were included in each group of this double-blinded split-mouth randomized clinical trial. The teeth were randomly divided into scaling and root planing + aPDT (test group) and scaling and root planing+sham aPDT (control group). The aPDT protocol consisted of incubation with 1% methylene blue for 5 min, rinsing, and application of a diode laser (wavelength of 660 nm, power of 100 mW) for 10 s. aPDT was repeated after 7 days. Bleeding on probing (BoP), probing depth (PD), gingival recession (GR), and clinical attachment loss (CAL) were recorded before and 3 months after treatment. A 5% significance level was adopted for statistical analysis., Results: Final PD was significantly (P = 0.02) lower in the test group (2.87 ± 1.40 mm) compared to control (3.12 ± 1.69 mm). The test group showed a significantly higher percentage of sites with PD≤4 mm and concomitant BoP compared to control (91%x86%;P < 0.001). At sites with baseline PD>4 mm, final PD and CAL were significantly (P = 0.01) lower in the test group (4.11 ± 1.66 and 4.89 ± 2.49 mm, respectively) compared to control (4.88 ± 1.99 and 5.89 ± 2.74 mm, respectively)., Conclusions: aPDT combined with scaling and root planning provided slightly better periodontal clinical results than the latter procedure alone, exerting a superior effect at sites with greater baseline PD. aPDT might be used as adjunctive treatment in grade C periodontitis affecting single-rooted teeth since it improves the response to conventional periodontal treatment., Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest related to this study., (Copyright © 2023 Elsevier B.V. All rights reserved.)
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- 2023
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21. Clinicopathological Features of Metastasis to the Oral and Maxillofacial Region-Multicenter Study.
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Kirschnick LB, Schuch LF, Gondak R, Rivero ERC, Gomes APN, Etges A, Tarquinio SBC, Mesquita RA, Caldeira PC, da Costa AAS, Mendonça EF, Dos Santos JN, Smit C, Robinson L, Tager EMJR, Mosqueda-Taylor A, Pontes HAR, de Andrade BAB, Fonseca TC, Abrahão AC, Agostini M, Romañach MJ, Alves FA, Jaguar GC, de Mendonça NF, Pinto MBR, da Silva LC, Lopes MA, Vargas PA, van Heerden W, Abreu LG, Martins MD, and Vasconcelos ACU
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- Male, Humans, Female, Middle Aged, Retrospective Studies, Mouth Neoplasms pathology, Adenocarcinoma secondary, Breast Neoplasms pathology, Oral Ulcer
- Abstract
Background: This study aimed to investigate the clinicopathological features of metastases in the oral and maxillofacial regions., Methods: In this retrospective study, biopsy records were obtained from referral centers for oral and maxillofacial diagnosis in Brazil, Guatemala, Mexico, and South Africa., Results: A total of 120 cases were evaluated. Of these, 53.78% affected female patients, with a mean age of 57.64 years. Intraosseous lesions were more frequent, particularly in the posterior region of the mandible (49.58%). Clinically, most cases presented with symptomatic swelling, with an average evolution time of 25 months. The clinical diagnostic hypothesis in most instances was that of a malignant lesion. Breast cancer was the most common primary tumor location in females, while lung origin was most common in males. In most cases, the primary cancer was an adenocarcinoma (44.73%). The follow-up period was available for 29 cases, and out of these, 20 had died due to the disease., Conclusion: Although this is a rare condition, clinicians should be aware that any oral lesions have the possibility of being metastatic, particularly in individuals with a previous history of cancer. The findings from this study could assist clinicians in prompt diagnosing these lesions and subsequent conducting oncologic assessments and treatment., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2023
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22. Salivary biomarkers to evaluate psychological disorders in oral lichen planus: A systematic review with meta-analysis.
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Pires ALPV, Alves LDB, da Silva AM, Arsati F, Lima-Arsati YBO, Dos Santos JN, Calumby RT, and Freitas VS
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- Humans, Adolescent, Hydrocortisone analysis, Cross-Sectional Studies, Saliva chemistry, Case-Control Studies, Biomarkers analysis, Lichen Planus, Oral complications, Lichen Planus, Oral diagnosis, Lichen Planus, Oral psychology
- Abstract
Objective: To analyze the use of salivary biomarkers to identify psychological disorders (anxiety, depression, and stress) in individuals with oral lichen planus (OLP)., Materials and Methods: The search was made in Medline/Pubmed, Scopus, Web of Science, Virtual Health Library, ProQuest, and by cross-reference. Original case-control, cross-sectional, and cohort studies, in human subjects (age ≥18 years) with clinical and histopathological diagnosis, with or without the use of psychometric scales, published until June 2021, were included. Random-effects meta-analyses, subgroup analyses, and sensitivity were performed. Publication bias was measured using the Egger test and visual inspection of the funnel plot., Results: A total of 67 articles were found, of which 12 were included in the meta-analysis. Among the different biomarkers identified, it was only possible to perform a meta-analysis for cortisol. The mean difference between salivary cortisol levels in patients with OLP compared to controls was 3.43 ng/ml (95% CI: 1.20-5.65) with I
2 = 98.9%. Studies considered outliers were removed and based on sensitivity analyses, results did not change, therefore, being stable., Conclusion: The studies analyzed demonstrated that cortisol was the most quantified biomarker to identify psychological disorders; however, DHEA, IgA, adiponectin, and α-amylase were also evaluated., (© 2022 Wiley Periodicals LLC.)- Published
- 2023
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23. Synchronous odontogenic tumors: A systematic review.
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Neumann BL, Só BB, Santos LG, Silveira FM, Wagner VP, Vargas PA, Dos Santos JN, Mosqueda-Taylor A, Fonseca FP, Schuch LF, and Martins MD
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- Humans, Maxilla pathology, Mandible pathology, Syndrome, Odontoma, Odontogenic Tumors epidemiology, Odontogenic Tumors pathology, Ameloblastoma pathology
- Abstract
This systematic review aimed to incorporate published information about synchronous odontogenic tumors (SOTs) with an analysis of the demographic and clinical characteristics from the cases published in the literature. Case reports and case series of SOT were searched in PubMed, Web of Science, Scopus, and EMBASE. A descriptive statistical analysis was performed. Twenty-eight studies comprising 30 cases of SOTs were included. Considering all cases published, SOTs mostly occurred simultaneously in the maxilla and mandible (n = 19/63.3%). Lesions were bifocal in 13 (43.3% of all the 30 cases) and multifocal in 17 cases (56.7% of all the 30 cases). All SOTs available in the literature presented the same type of lesion, and two of them also involved another different SOT (n = 2/6.7% of all the 30 cases). Out of all published cases, the most frequent SOTs in the literature were odontomas (n = 10/33.3% of all the 30 cases), squamous odontogenic tumors (OTs) (n = 8/26.7% of all the 30 cases), calcifying epithelial OTs (n = 8/26.7% of all the 30 cases), and adenomatoid OTs (n = 2/6.7% of all the 30 cases). Considering all SOTs cases included, the overall recurrence was 13.3%. Inside a subgroup of the lesion, synchronous calcifying epithelial OT presented the highest (25%). Five cases (16.7% of all the 30 cases) had a previously associated syndrome, with two cases of Schimmelpenning syndrome being reported. Among published SOTs, odontomas were the most common. All SOTs available in the scientific literature showed the same type of OT and mainly affected both jaws simultaneously. Only a few of these cases were associated with a syndrome., (© 2022 Wiley Periodicals LLC.)
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- 2023
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24. Can SARS-CoV-2 screening in oral biopsies aid epidemiological surveillance?
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Martins-Chaves RR, Diniz MG, Miguita L, Felix FA, Rocha FF, Fonseca PLC, Geddes VEV, Menezes D, Moreira RG, Liborio-Kimura T, Câmara J, da Costa Mendes TC, Pontes HAR, Pontes FSC, de Freitas Gonçalves TO, Fonsêca TC, Abrahão AC, Romañach MJ, Alves APNN, Pereira KMA, da Cruz Perez DE, de Amorim Carvalho EJ, Dos Santos JN, de Aquino Xavier FC, Giffoni FC, de Souza AH, Gomes CC, de Sousa SF, Fonseca FP, de Souza RP, Aguiar RS, and Gomez RS
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- Humans, Phylogeny, Pandemics, SARS-CoV-2 genetics, COVID-19 diagnosis, COVID-19 epidemiology
- Abstract
Background: Three years after the first confirmed COVID-19 case in Brazil, the outcomes of Federal government omissions in managing the crisis and anti-science stance heading into the pandemic have become even more evident. With over 36 million confirmed cases and nearly 700 000 deaths up to January 2023, the country is one of the hardest-hit places in the world. The lack of mass-testing programs was a critical broken pillar responsible for the quick and uncontrolled SARS-CoV-2 spread throughout the Brazilian population. Faced with this situation, we aimed to perform the routine SARS-CoV-2 screening through RT-qPCR of oral biopsies samples to aid in the asymptomatic epidemiological surveillance during the principal outbreak periods., Methods: We analyzed 649 formalin-fixed paraffin-embedded oral tissue samples from five important oral and maxillofacial pathology laboratories from the north, northeast, and southeast geographic regions of Brazil. We also sequenced the whole viral genome of positive cases to investigate SARS-CoV-2 variants., Results: The virus was detected in 9/649 analyzed samples, of which three harbored the Variant of Concern Alpha (B.1.1.7)., Conclusion: Although our approach did not value aiding asymptomatic epidemiological surveillance, we could successfully identify a using FFPE tissue samples. Therefore, we suggest using FFPE tissue samples from patients who have confirmed diagnosis of SARS-CoV-2 infection for phylogenetic reconstruction and contraindicate the routine laboratory screening of these samples as a tool for asymptomatic epidemiological surveillance., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2023
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25. Photodynamic therapy as an adjunctive treatment for Grade C periodontitis in molar teeth: a preliminary trial.
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Coelho TDRC, Pinto Filho JM, Ribeiro Caponi LSFE, Soares JDM, Dos Santos JN, and Cury PR
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- Humans, Dental Scaling methods, Combined Modality Therapy, Root Planing methods, Molar, Photosensitizing Agents therapeutic use, Periodontitis drug therapy, Photochemotherapy methods, Chronic Periodontitis
- Abstract
Objective: The present study aimed to evaluate the local effect of antimicrobial photodynamic therapy (aPDT) as adjunctive treatment for Grade C periodontitis in molars teeth., Method and Materials: Thirty-eight molar teeth from 11 patients were included in this split-mouth randomized clinical trial. The teeth were randomly divided into two groups (n = 19 each) that were submitted to conventional scaling and root planing in addition to aPDT (test group) and to scaling and root planing in addition to aPDT simulation (control group). The following periodontal parameters were obtained prior to treatment (baseline) and at 3 months after the intervention: bleeding on probing, probing depth, gingival recession, clinical attachment level, and furcation level. A 5% significance level was adopted in the statistical analysis., Results: Both groups exhibited improvement in the clinical parameters (P < .001). However, the reductions in bleeding on probing and probing depth were greater in the test group (P < .001). There were no significant differences in the other clinical parameters between groups (P ≥ .130)., Conclusions: Molar teeth treated with aPDT and scaling and root planing showed superior clinical improvement compared to those submitted only to scaling and root planing. aPDT may be used as adjunctive treatment for Grade C periodontitis affecting molar teeth since it seems to improve the clinical response to conventional debridement.
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- 2023
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26. Effects of Violet and IR LED Light on mast cell degranulation: in vivo study in a murine model.
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Araújo GLB, Soares LGP, Santos AS, Cangussú MCT, Dos Santos JN, and Pinheiro ALB
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- Animals, Rats, Disease Models, Animal, Phototherapy, Rats, Wistar, Cell Degranulation radiation effects, Mast Cells radiation effects
- Abstract
The aim of this study was to evaluate the influence of IR (λ850 ± 10 nm) and violet (λ405 ± 10 nm) LED phototherapy on total mast cells counts and its ability to influence mast cell degranulation. For this, 27 Wistar rats were used and were randomly distributed into three groups: control, IR LED, and violet LED. When indicated, irradiation done and they were sacrificed, had their tongue removed immediately, 20-min, 45-min, and 2-h after irradiation. Samples were processed to wax, cut, and stained with Toluidine Blue. Intact and degranulated mast cells were counted under light microscopy, and statistical analysis was carried out. In the superficial connective tissue and muscular tissues, violet LED light caused a significant increase in both total number and degranulated mast cells when compared to the control group immediately after irradiation. The degranulation indexes were higher in the groups irradiated with Violet light, both in superficial connective tissue and muscular tissues in relation to the timing. Irradiation with IR LED caused immediate increase in the total number and degranulated of mast cells when compared to the control group only in the superficial connective tissue. In all times observed, the highest total amount of mast cells was seen immediately after irradiation, except in the muscular tissue, which presented the highest amount after 20-min. It was concluded that IR and violet LED light were able to increase the number of mast cells and inducing degranulation in oral mucosa. However, considering that violet LED light can be harmful in periodontal disease, it seems that the use of IR LED light could be the best option in Dentistry., (© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
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- 2023
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27. Gingival neoplasms: a multicenter collaborative study of 888 patients in Brazil.
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Santos-Leite ÉG, Louredo BV, de Souza LL, Pontes HA, Pontes FS, Dos Santos JN, Henriques ÁC, de Castro JF, Carvalho EJ, Leonel AC, Albuquerque RF, Gonçalves TO, Abrahão AC, Agostini M, Romañach MJ, Carrard VC, Martins MD, Dos Santos TC, Pires FR, Santos-Silva AR, Lopes MA, Vargas PA, and Perez DE
- Subjects
- Male, Humans, Aged, Middle Aged, Female, Brazil epidemiology, Ulcer diagnosis, Diagnosis, Differential, Retrospective Studies, Gingival Neoplasms pathology, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell epidemiology, Carcinoma, Squamous Cell pathology, Oral Ulcer
- Abstract
Background: To evaluate the prevalence and clinicopathological features of a large series of gingival neoplasms in Brazil., Material and Methods: All gingival benign and malignant neoplasms were retrieved from the records of six Oral Pathology Services in Brazil, during a 41-year period. Clinical and demographic data, clinical diagnosis, and histopathological data were collected from the patients' clinical charts. For statistical analysis, the chi-square, median test of independent samples and the U Mann-Whitney tests were used, considering a significance of 5%., Results: From 100,026 oral lesions, 888 (0.9%) were gingival neoplasms. There were 496 (55.9%) males, with a mean age of 54.2 years. Most cases (70.3%) were malignant neoplasms. Nodules (46.2%) and ulcers (38.9%) were the most common clinical appearance for benign and malignant neoplasms, respectively. Squamous cell carcinoma (55.6%) was the most common gingival neoplasm, followed by squamous cell papilloma (19.6%). In 69 (11.1%) malignant neoplasms, the lesions were clinically considered to be inflammatory or of infectious origin. Malignant neoplasms were more common in older men, appeared with larger size, and with a time of complaint shorter than benign neoplasms (p<0.001)., Conclusions: Benign and malignant tumors may appear as nodules in gingival tissue. In addition, malignant neoplasms, especially squamous cell carcinoma, should be considered in the differential diagnosis of persistent single gingival ulcers.
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- 2023
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28. Is micronucleus test a suitable method for monitoring oral mucosa exposed to dental bleachings in smokers?
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Takeshita WM, Dos Santos JN, de Andrade CR, and Ribeiro DA
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- Humans, Micronucleus Tests, Micronuclei, Chromosome-Defective, Smoking adverse effects, Mouth Mucosa, Smokers
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- 2023
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29. Impact of serum vitamin D levels on periodontal healing outcomes: A preliminary cohort study.
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Ribeiro LSFE, Araujo NS, Zilli Vieira CL, Dos Santos JN, and Cury PR
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- Humans, Cohort Studies, Periodontal Pocket therapy, Wound Healing, Vitamin D, Gingival Hemorrhage, Periodontal Attachment Loss therapy, Periodontitis therapy
- Abstract
Objective: To evaluated the relationship between serum vitamin D levels and periodontal healing outcomes in patients with mild or moderate periodontitis., Methods: Serum vitamin D levels and periodontal pockets and gingival bleeding were evaluated in 51 patients before and 6 months after non-surgical periodontal treatment. A t-test and chi-square test were used to analyse the data (p ≤ 0.05)., Results: The mean reduction of periodontal pocket depth was statistically significant higher in patients with vitamin D ≥30 ng/ml than those with <30 ng/ml (CI = -0.23-0.42, p = 0.05), but not clinically significant. Vitamin D level was not associated with the percentage of sites of gingival bleeding on probing at the final evaluation (OR = 0.58, 95% CI = 0.17-1.99, p = 0.39)., Conclusion: Lower serum vitamin D level was associated with a slightly reduced periodontal healing after non-surgical periodontal therapy, which should be further investigated in a larger population., (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2023
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30. Epithelial salivary gland tumors in pediatric patients: An international collaborative study.
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Quixabeira Oliveira GA, Pérez-DE-Oliveira ME, Robinson L, Khurram SA, Hunter K, Speight PM, Kowalski LP, Lopes Pinto CA, Sales De Sá R, Mendonça EF, Sousa-Neto SS, de Carlucci Junior D, Mariano FV, Altemani AMAM, Martins MD, Zanella VG, Perez DEDC, Dos Santos JN, Romañach MJ, Abrahão AC, Andrade BAB, Pontes HAR, Jorge Junior J, Santos-Silva AR, Lopes MA, Van Heerden WFP, and Vargas PA
- Subjects
- Female, Humans, Child, Adolescent, Infant, Newborn, Infant, Child, Preschool, Young Adult, Adult, Retrospective Studies, Salivary Glands surgery, Salivary Glands pathology, Salivary Gland Neoplasms epidemiology, Salivary Gland Neoplasms surgery, Adenoma, Pleomorphic epidemiology, Adenoma, Pleomorphic surgery, Adenoma, Pleomorphic pathology, Carcinoma, Mucoepidermoid pathology
- Abstract
Objective: Salivary gland tumors (SGT) are a diverse group of uncommon neoplasms that are rare in pediatric patients. This study aimed to characterize the clinicopathological profile of pediatric patients affected by SGT from a large case series derived from an international group of academic centers., Study Design: A retrospective analysis of pediatric patients with SGT (0-19 years old) diagnosed between 2000 and 2021 from Brazil, South Africa, and the United Kingdom was performed. SPSS Statistics for Windows was used for a quantitative analysis of the data, with a descriptive analysis of the clinicopathological characteristics and the association between clinical variables and diagnoses., Results: A total of 203 cases of epithelial SGT were included. Females were slightly more commonly (56.5%), with a mean age of 14.1 years. The palate was the most common site (43.5%), followed by the parotid gland (29%), lip (10%), and submandibular gland (7.5%). The predominant clinical presentation was a flesh-colored, smooth, and painless nodule. Pleomorphic adenoma (PA) was the most frequently diagnosed SGT (58.6%), followed by mucoepidermoid carcinoma (MEC) (26.6%). Surgery (90.8%) was the favored treatment option., Conclusions: Benign SGT in pediatric patients are more commonly benign than malignant tumors. Clinicians should keep PA and MEC in mind when assessing nodular lesions of possible salivary gland origin in pediatric patients., Competing Interests: Declaration of competing interest The authors declare no conflict of interest or any commercial associations., (Copyright © 2023 Elsevier B.V. All rights reserved.)
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- 2023
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31. Heterogeneity and versatility of the extracellular matrix during the transition from pleomorphic adenoma to carcinoma ex pleomorphic adenoma: cumulative findings from basic research and new insights.
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Scarini JF, de Lima-Souza RA, Lavareze L, Ribeiro de Assis MCF, Damas II, Altemani A, Egal ESA, Dos Santos JN, Bello IO, and Mariano FV
- Abstract
Pleomorphic adenoma (PA) is the most common salivary gland tumor, accounting for 50%-60% of these neoplasms. If untreated, 6.2% of PA may undergo malignant transformation to carcinoma ex-pleomorphic adenoma (CXPA). CXPA is a rare and aggressive malignant tumor, whose prevalence represents approximately 3%-6% of all salivary gland tumors. Although the pathogenesis of the PA-CXPA transition remains unclear, CXPA development requires the participation of cellular components and the tumor microenvironment for its progression. The extracellular matrix (ECM) comprises a heterogeneous and versatile network of macromolecules synthesized and secreted by embryonic cells. In the PA-CXPA sequence, ECM is formed by a variety of components including collagen, elastin, fibronectin, laminins, glycosaminoglycans, proteoglycans, and other glycoproteins, mainly secreted by epithelial cells, myoepithelial cells, cancer-associated fibroblasts, immune cells, and endothelial cells. Like in other tumors including breast cancer, ECM changes play an important role in the PA-CXPA sequence. This review summarizes what is currently known about the role of ECM during CXPA development., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Scarini, de Lima-Souza, Lavareze, Ribeiro de Assis, Damas, Altemani, Egal, dos Santos, Bello and Mariano.)
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- 2023
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32. Interrogation of TERT promoter hotspot mutations in ameloblastoma and ameloblastic carcinoma.
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Magalhães MCSV, Felix FA, Guimarães LM, Dos Santos JN, de Marco LA, Gomez RS, Gomes CC, and de Sousa SF
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- Humans, Mutation, Ameloblastoma genetics, Telomerase genetics, Telomerase metabolism, Odontogenic Tumors genetics, Carcinoma
- Abstract
Background: TERT promoter mutations increase telomerase activity, conferring cell immortality. The coexistence of TERT promoter mutations with BRAFV600E is associated with aggressiveness. Ameloblastoma and ameloblastic carcinoma are infiltrative neoplasms that harbor BRAFV600E; however, it remains unknown if these odontogenic tumors also show TERT promoter mutations., Methods: Genomic DNA of paraffin-embedded ameloblastomas (n = 6) and ameloblastic carcinomas (n = 3) were Sanger-sequenced to assess the hotspot TERT promoter mutations C228T and C250T. BRAFV600E status was screened by TaqMan allele-specific quantitative polymerase chain reaction., Results: None of the samples harbored TERT promoter mutations. The BRAFV600E mutation was positive in 3 of 6 of ameloblastomas and in 1 of 3 of ameloblastic carcinomas., Conclusion: The absence of TERT promoter mutation in the samples indicates that this molecular event is not relevant to the tumors' pathogenesis. Further studies are necessary to explore undefined genetic or epigenetic mechanisms related to TERT-upregulation in ameloblastoma, and the telomerase activity in ameloblastic carcinoma., (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2023
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33. Morphological and molecular characterization of Cosmocercoides amapari n. sp. (Nematoda: Cosmocercidae), parasitic in hylid frogs from the Brazilian Amazon.
- Author
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Rebêlo GL, Santos AN, Tavares-Costa LFS, Dias-Souza MR, Müller MI, Jesus RF, Costa-Campos CE, Dos Santos JN, and Melo FTV
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- Animals, Brazil, Phylogeny, Anura parasitology, Parasites, Nematoda, Ascaridida genetics, Ascaridida anatomy & histology
- Abstract
Cosmocercoides Wilkie, 1930 are gastrointestinal parasites commonly found in amphibians and reptiles, with 4 species reported from the Neotropical region. In the present study, a new species of Cosmocercoides , namely Cosmocercoides amapari n. sp. is described using integrated approaches such as light and scanning microscopy, and DNA sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene. The specimens were collected from the large intestine of 3 species of hylid frogs in Amapá, Brazil. The new species can be distinguished from its congeners by morphological traits, including the pattern of caudal papillae, absence of the gubernaculum, 2 poorly sclerotized spicules, presence of lateral alae and somatic papillae along the body. In addition, our molecular analyses and phylogenetic reconstructions strongly support the status of the new taxon, which clustered poorly with a large clade of Cosmocerca spp. Cosmocercoides amapari n. sp. is the 29th species of the genus, the 5th from the Neotropical region, the third reported in Brazil, the second described from the Amazon region and the first belonging to the Neotropical region with molecular data.
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- 2023
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34. Vogtnema asymmetrica n. gen., n. sp. (Nematoda: Cosmocercoidea: Atractidae) parasite of Rhinoclemmys punctularia (Daudin) (Testudines: Geoemydidae) from eastern Amazon, Brazil.
- Author
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Jesus RF, Santos AN, Nandyara B, Dos Santos JN, and Melo FTV
- Subjects
- Animals, Brazil, Male, Species Specificity, Ascaridida, Nematoda, Parasites, Turtles parasitology
- Abstract
Nematodes collected from the stomach and large intestine of Rhinoclemmys punctularia (Daudin) from the eastern Amazon, Brazil, are assigned to a new genus, Vogtnema n. gen., allocated in the family Atractidae (Cosmocerdoidea). Vogtnema n. gen. differs from all other genera of Atractidae based on the combination of the following morphological characters: four lips V-shaped (two subdorsal and two subventral) without sclerotized structures associated, absence of sclerotized formations and/or pieces surrounding the oral opening, oral aperture surrounded by flange internal membranous structures; in males the larger spicule is alate. The type species Vogtnema asymmetrica n. gen., n. sp. has the five precloacal pairs of papillae situated on the right side of the body that are more prominent than those on the left side. We also provide an identification key to the genera of the family Atractidae., (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
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- 2022
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35. Chlorhexidine mouthwash reduces the salivary viral load of SARS-CoV-2: A randomized clinical trial.
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Costa DD, Brites C, Vaz SN, de Santana DS, Dos Santos JN, and Cury PR
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- Humans, Chlorhexidine therapeutic use, Mouthwashes therapeutic use, Viral Load, SARS-CoV-2, COVID-19
- Abstract
Objectives: This study aimed to evaluate the effect of 0.12% chlorhexidine gluconate on the salivary load of SARS-CoV-2., Materials and Methods: A randomized, double-blind, placebo-controlled trial was performed on 100 participants positive for SARS-CoV-2. In the test group (n = 50), volunteers gargled with a mouthwash containing 15 ml of 0.12% chlorhexidine gluconate for 1 min, while the control group (n = 50) used a placebo. Saliva samples were obtained before (baseline) and 5 and 60 min after using the solutions. Real-time reverse transcription polymerase chain reaction assays (qRT-PCR) were carried out and the cycle threshold (Ct) was computed. The chi-square test and t-test were used for group comparison (p ≤ 0.05)., Results: The differences in Ct values between the 5-min evaluation and baseline (test group: 2.19 ± 4.30; control: -0.40 ± 3.87, p = 0.002) and between 60 min and baseline (test group: 2.45 ± 3.88; control: 0.76 ± 4.41, p = 0.05) were significantly greater in the test group, revealing a reduction of viral load. Furthermore, there was a reduction in the load of SARS-CoV-2 in 72% of the volunteers using chlorhexidine versus 30% in the control group (p = 0.001)., Conclusions: Chlorhexidine gluconate (0.12%) was effective in reducing salivary SARS-CoV-2 load for at least 60 min., (© 2021 Wiley Periodicals LLC.)
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- 2022
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36. Impact of the COVID-19 pandemic on public University laboratories of oral and maxillofacial pathology: A Brazilian multicenter study.
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Caldeira PC, Schuch LF, Tavares TS, Santos-Silva AR, Vargas PA, Pérez-de-Oliveira ME, de Almeida OP, de Souza LB, Gonzaga AKG, Mendonça EF, Sousa-Neto SS, Rivero ERC, Modolo F, Dos Santos JN, Manieri PR, Nonaka CFW, Alves PM, Libório-Kimura TN, Mendes TCDC, Etges A, Tarquinio SBC, Gomes APN, Abreu LG, and Vasconcelos ACU
- Subjects
- Humans, Brazil epidemiology, Cross-Sectional Studies, Laboratories, Pandemics, Universities, COVID-19 epidemiology, Pathology, Oral
- Abstract
Objective: To evaluate the impact of the COVID-19 pandemic on the number of diagnoses of oral and maxillofacial lesions in public laboratories after one year of COVID-19 outbreak in Brazil., Material and Methods: This is a cross-sectional study. Biopsies submitted to histopathologic examination from March 2019 to February 2020 (pre-pandemic period) and from April 2020 to March 2021 (pandemic period) in nine Brazilian public oral pathology laboratories were retrieved and the number of diagnoses, types of lesion, and percentage changes during both periods were analyzed., Results: There were 7389 diagnoses in the pre-pandemic period and 2728 in the pandemic era, indicating a reduction of 63.08%. The reduction was 64.23% for benign lesions and 49.48% for malignant lesions, with a 50.64% reduction in squamous cell carcinoma. The largest decreases were observed in April 2020 and January 2021., Conclusion: An important reduction in the diagnoses of benign and malignant lesions was noted in the Brazilian public oral pathology laboratories during the first year of the COVID-19 pandemic., (© 2022 Wiley Periodicals LLC.)
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- 2022
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37. Morphological Changes in Major Salivary Glands in Mice Treated With a Choline and Methionine Deficient Diet.
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DA Silva Thomaz M, Ribeiro DA, Dos Santos JN, and Nagaoka MR
- Subjects
- Animals, Atrophy pathology, Diet, Male, Mice, Racemethionine, Salivary Glands pathology, Choline, Methionine
- Abstract
Background/aim: It has been shown that the methionine-choline deficient (MCD) diet induces hepatocarcinogenesis, but not in extrahepatic organs, such as the testis, and pancreas, although may increase chemical-induced carcinogenesis in the colon, mammary gland, esophagus, and pancreas. Accumulating evidence suggests that salivary glands are very susceptible to stress conditions, such as radiation, hyperglycemia, and exposure to xenobiotics in vivo. This study aimed to analyze the histological changes on the major salivary glands (parotid, submandibular, and sublingual) after MCD diet administration., Materials and Methods: Male Swiss mice were submitted to ad libitum access to the control (AIN-76) or MCD diet for 28 days. The rebound group received the MCD diet for 24 days and the control diet for 10 days. Using the AxioImager A2 microscope, the hematoxylin-eosin (HE) stained specimens (4 mm) were evaluated for tissue degeneration, nuclear hyperchromatism and atrophy., Results: In the parotid gland from the MCD group, tissue degeneration, pyknosis, apoptosis and atrophy were observed, which remained in the rebound group, associated with hyperchromatism. In the submandibular gland from both MCD and rebound groups, severe tissue disorganization was associated with cell pleomorphism, hyperchromatic cells, apoptosis, increased eosinophilia, and inflammatory infiltrate. Finally, in the sublingual gland, there were no histological alterations in the experimental groups compared to the control., Conclusion: MCD can induce pre-neoplastic changes in the mouse parotid and submandibular glands, which are not reversed by a change in the diet., (Copyright © 2022, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
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- 2022
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38. Phylogenetic relationships of Ochoterenatrema Caballero, 1943 (Digenea: Lecithodendriidae) with descriptions of two new species.
- Author
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Fernandes TF, Dos Santos JN, de Vasconcelos Melo FT, Achatz TJ, McAllister CT, Carrion Bonilla C, and Tkach VV
- Subjects
- Animals, Brazil, Phylogeny, RNA, Ribosomal, 28S genetics, Chiroptera parasitology, Trematoda, Trematode Infections parasitology, Trematode Infections veterinary
- Abstract
Ochoterenatrema is a small genus of lecithodendriid digeneans that includes six species parasitic in Neotropical bats in North and South America. Members of this genus are characterized by the presence of a pseudogonotyl formed by thickened tegument on the left side of the ventral sucker. We examined morphology of specimens belonging to five species of Ochoterenatrema from Brazil, Ecuador and the USA and describe two new Ochoterenatrema spp. (Ochoterenatrema sphaerula n. sp. and Ochoterenatrema gracilis n. sp.). These new species are readily differentiated from their congeners by a combination of morphological characters, including the distribution of vitelline follicles and the body shape. We have provided the first DNA sequences for Ochoterenatrema. Newly generated partial nuclear 28S rDNA and mitochondrial cox1 gene sequences were used to differentiate among species and study the phylogenetic affinities of Ochoterenatrema spp., including one of the new species, O. gracilis n. sp., as well as Ochoterenatrema diminutum, Ochoterenatrema fraternum and Ochoterenatrema cf. labda. The phylogeny of the Microphalloidea based on 28S had well-supported topology, particularly at the family level. The Ochoterenatrema clade was strongly supported; however, the internal topology of the clade was weakly supported. Comparison of sequences revealed 0.4-1.3% interspecific divergence in 28S and 9.1-19.7% interspecific divergence in cox1 among Ochoterenatrema spp. We hypothesize that extremely diverse fauna of bats in South and Central America harbors multiple undescribed species of Ochoterenatrema. Several lecithodendriid genera from bats and other hosts are yet to be included in future molecular phylogenetic analyses to test the monophyly of this extremely diverse digenean family and analyze evolutionary affinities of its constituent taxa., (Copyright © 2022 Elsevier B.V. All rights reserved.)
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- 2022
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39. Expression of Matrix Metalloproteinases 7 and 9, Desmin, Alpha-Smooth Muscle Actin and Caldesmon, in Odontogenic Keratocyst Associated with NBCCS, Recurrent and Sporadic Keratocysts.
- Author
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Loreto C, Polizzi A, Filetti V, Pannone G, Dos Santos JN, Venezia P, Leonardi R, and Isola G
- Subjects
- Actins metabolism, Calmodulin-Binding Proteins, Desmin metabolism, Humans, Matrix Metalloproteinase 7 metabolism, Matrix Metalloproteinase 9 metabolism, Muscle, Smooth metabolism, Basal Cell Nevus Syndrome metabolism, Basal Cell Nevus Syndrome pathology, Odontogenic Cysts metabolism, Odontogenic Cysts pathology
- Abstract
Nevoid basal cell carcinoma syndrome (NBCCS) associated odontogenic keratocysts (OKCs) show more aggressive behavior and it has a higher frequency of relapse than non-syndromic OKCs. Stromal myofibroblasts (MFs), characterized by α-smooth muscle actin (αSMA), desmin and caldesmon expression, and metalloproteinases (MMPs) have an essential role in the remodeling of the extracellular matrix (ECM). The aim of the study is to analyze the immunohistochemical expression of MMP-7, MMP-9, αSMA and other new markers in the study of OKCs MFs such as desmin and caldesmon in NBCCS-associated OKCs compared to recurrent and sporadic keratocysts. Fourty 40 patients (23 M and 17 F) underwent surgery to remove the OKCs. The histological sections in paraffin were incubated with markers antibodies and a semi-quantitative score was used to evaluate the immunoreactivity. Densitometric analysis showed a very significantly increased expression of αSMA, caldesmon, MMP-7 and MMP-9 in NBCCS-OKCs compared to non-syndromic OKCs (p < 0.001). However, desmin showed a not significant increased expression in non-syndromic OKC compared to NBCCS-OKCs specimens in which desmin was slightly or not at all expressed. NBCSS-OKCs showed a greater distribution of MFs compared to the other OKCs subtypes. Further studies will be needed to evaluate whether the different expressions of these markers can be correlated to a different clinical behavior.
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- 2022
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40. Oral and Maxillofacial Neuroendocrine Carcinoma: A Systematic Review.
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Schuch LF, Schmidt TR, de Oliveira Zigmundo G, Kirschnick LB, Silveira FM, Martins MAT, Carlos R, Dos Santos JN, Fonseca FP, Vargas PA, Wagner VP, and Martins MD
- Subjects
- Adult, Aged, Humans, Immunohistochemistry, Male, Middle Aged, Prognosis, Carcinoma, Neuroendocrine pathology
- Abstract
The aim of the present study was to integrate the available data published in the literature on oral and maxillofacial neuroendocrine carcinomas concerning the demographic, clinical and histopathological features of this condition. An electronic search with no publication date restriction was undertaken in April 2021 in four databases. Eligibility criteria included reports published in English having enough data to confirm a definite diagnosis, always showing a neuroendocrine marker. Cases originating in the oropharynx, including base of the tongue and tonsils, were excluded. Outcomes were evaluated by the Kaplan-Meier method along with Cox regression. Twenty-five articles (29 cases) from nine different countries were detected. Mean patient age was 56.3 (± 17.5) years, with a slight male predilection. Symptomatology was present in 72.2% of informed cases. Regarding clinical presentation, a non-ulcerated nodule located in the gingiva with a mean size of 3.4 (± 2.0) cm was most frequently reported. Concomitant metastasis was identified in seven individuals. Histopathologically, most neoplasms were of the small cell type, and immunohistochemistry for both epithelial and neuroendocrine differentiation was used in 65.5% cases. Radical surgery was the treatment of choice in almost all cases, with or without adjuvant therapy. Mean follow-up was 20.5 (± 21.2) months, and only four patients developed recurrences. Eleven (44.0%) individuals died due to the disease. Ulcerated lesions were a prognostic factor. This study provides knowledge that can assist surgeons, oncologists, and oral and maxillofacial pathologists with the diagnosis and management of neuroendocrine carcinomas. Our findings demonstrated that the long-term prognosis of this lesion continues to be poor., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2022
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41. Histological evaluation of skin lesions induced by Leishmania braziliensis treated by PACT using Laser light and 1.9 dimethyl-methylene blue.
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Fagnani SRCA, de Oliveira SCPS, Monteiro JSC, Sampaio FJP, Crugeira PJL, Dos Santos JN, and Pinheiro ALB
- Subjects
- Animals, Inflammation drug therapy, Lasers, Methylene Blue pharmacology, Methylene Blue therapeutic use, Mice, Photosensitizing Agents pharmacology, Photosensitizing Agents therapeutic use, Leishmania braziliensis, Photochemotherapy methods
- Abstract
This study aimed to perform a histological evaluation in skin lesions caused by Leishmania braziliensis after PACT treatment using Laser associated with 1.9. dimethyl methylene blue BALB/c mouse ear infection model was used. A total of 40 animals were assigned into two groups considering time intervals at 5 and 10 weeks and subdivided into four subgroups: Control, Photosensitizer, Laser and PACT. Two therapeutic interventions were performed after the 5th week of infection at 48 h intervals. 1.9 Dimethyl methylene blue was used as a photosensitizer at the concentration of 7 ng/mL, with a non-invasive topical administration method associated with Laser (λ = 660 nm, 40 mW, 12 J/cm
2 ). Sample collection occurred 5 or 10 weeks after therapeutic interventions. The main histological findings were observed in the laser and PACT groups at the 10-week evaluation. The Laser group showed reduced lymphoplasmacytic inflammation and histiocytes (p = 0.0079). The PACT group showed reductions in lymphoplasmacytic inflammation at 5 and 10 weeks, discrete reduction of histiocytes and a higher percentage of tissue remodeling. PACT with non-invasive topical administration of the photosensitizer was able to reduce lymphoplasmacytic inflammation and increase tissue remodeling in leishmaniosis skin lesions. This protocol may be easily used in humans and clinical trial shall be carried out to confirm the animal's findings., (Copyright © 2022 Elsevier B.V. All rights reserved.)- Published
- 2022
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42. Clear cell odontogenic carcinoma involving the periapical region.
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Manieri PR, Melo LA, Maranhão SC, Mosqueda-Taylor A, Cury PR, Martins MD, and Dos Santos JN
- Subjects
- Humans, Carcinoma, Odontogenic Cysts pathology, Odontogenic Tumors diagnosis, Odontogenic Tumors pathology
- Published
- 2022
- Full Text
- View/download PDF
43. Is micronucleus assay in oral exfoliated cells a suitable biomarker for predicting cancer risk in individuals with oral potentially malignant disorders? A systematic review with meta-analysis.
- Author
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de Souza DV, Dos Anjos Rosario B, Takeshita WM, de Barros Viana M, Nagaoka MR, Dos Santos JN, and Ribeiro DA
- Subjects
- Biomarkers, Humans, Micronucleus Tests, Mouth Neoplasms, Precancerous Conditions
- Abstract
This systematic review (SR) with meta-analysis aimed to evaluate the scientific data related to cytogenetic damage in oral exfoliated cells of patients diagnosed with oral potentially malignant disorders (OPMDs). The SR was conducted according to the PRISMA-P guidelines. The PICOS (Participants, Intervention, Comparison, Outcome, and Study Design) strategy was used to answer the question: "Is micronucleus assay in oral exfoliated cells a suitable biomarker for predicting cancer risk in individuals with OPMDs?" The search strategy was performed in the following electronic databases: PubMed, Medline, Scopus and Web of Science. The comparisons were defined as standardized mean difference (SMD), and 95% confidence intervals (CI). The quality of included studies was assessed using the EPHPP (Effective Public Health Practice Project). The GRADE tool was also utilized to assess the quality of evidence of the SR. A total of 110 potentially relevant studies were selected through the search strategy. After screening titles and abstracts, 20 full-text manuscripts were assessed for eligibility and three observational studies were included in the meta-analysis. After reviewing the 20 studies, 13 were considered weak. The meta-analysis data revealed a statistically significant difference in oral micronucleated cells by patients with OPMDs when compared to control (SMD=1.77, 95% CI, 0.36-3.18, p = 0.01), with a Tau
2 = 1.97; Chi2 = 66.64, and p < 0.001. Patients with OPMDs had a positive response related to mutagenicity in oral cells compared to control patients. However, SR was not able to validate the micronucleus assay as a putative biomarker in individuals with oral potentially malignant disorders since the majority of studies were considered weak based on high risk of bias., (Copyright © 2022 Elsevier GmbH. All rights reserved.)- Published
- 2022
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44. Impact of photobiomodulation therapy on the morphological aspects of submandibular gland submitted to excretory duct ligation and hypothyroidism: an animal study.
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Pimentel ACL, Rodriguez TT, Martins MD, Filho LCR, Mota IFS, de Carvalho Monteiro JS, Manieri PR, Pinheiro ALB, Cury PR, and Dos Santos JN
- Subjects
- Animals, Ligation, Male, Rats, Rats, Wistar, Salivary Ducts, Submandibular Gland, Hypothyroidism etiology, Hypothyroidism radiotherapy, Low-Level Light Therapy
- Abstract
The aim of this study was to evaluate the impact of photobiomodulation therapy (PBMT) on histomorphological aspects of submandibular gland (SMG) submitted to salivary gland duct obstruction in hypothyroid rats. Fifty-six male Wistar rats (250 to 300 g) were divided into 4 groups (n = 14): euthyroid (EU), EU + PBMT, hypothyroid (HYPO), and HYPO + PBMT. Duct obstruction of the left submandibular gland (LSMG) was performed in all animals by a ligature procedure. For the induction of hypothyroidism, total thyroidectomy was performed. PBMT groups received irradiation with AlGaInP diode laser (808 nm, 0.04 W, 0.04cm
2 spot size, 60 s, 2.4 J per point, 60 J/cm2 , 1 W/cm2 ). Irradiation was performed immediately, 24 h, and 48 h after the obstruction of the salivary gland duct, in one point, extra oral and perpendicular to the gland. Animals were sacrificed after 24 h and 72 h after duct ligature. Our results indicated that salivary duct obstruction and hypothyroidism caused negative modifications on the salivary glands' histomorphology, especially acinar atrophy, after 24 h and 72 h. HYPO + PBMT showed a significant reduction of the inflammatory infiltrate, congested blood vessels, and acinar atrophy in the SMG submandibular salivary gland in 72 h compared to 24 h (p < 0.05). In conclusion, obstruction of the salivary gland excretory duct and hypothyroidism causes severe sialoadenitis with expressive atrophy of the glandular parenchyma. However, PBMT was able to modulate the inflammatory process and delaying acinar atrophy. This study provided insights to better understand the role of the PBMT on the altered salivary gland by duct ligation and associate hypothyroidism., (© 2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)- Published
- 2022
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45. Perinatal and post-weaning exposure to an obesogenic diet promotes greater expression of nuclear factor-κB and tumor necrosis factor-α in white adipose tissue and hypothalamus of adult rats.
- Author
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Dos Santos LS, de Matos RJB, Cordeiro GDS, Dos Santos JN, Perez GDS, Gonçalves MDS, Ribeiro IO, and Barreto Medeiros JM
- Subjects
- Adipose Tissue metabolism, Adipose Tissue, White metabolism, Animals, Body Weight, Diet, High-Fat adverse effects, Female, Hypothalamus metabolism, Lactation, Pregnancy, Rats, Rats, Wistar, Weaning, NF-kappa B genetics, NF-kappa B metabolism, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism
- Abstract
Aim: To analyze the effects of exposure to a high-fat diet during the perinatal period and after weaning on white adipose tissue accumulation and gene expression of TNF- α and NF- κB. Method: Wistar female rats were fed with high-fat (H) or control (C) diet during pregnancy and lactation. The offspring were allocated into four groups: Control Control (CC), offspring of mothers GC, fed a control diet after weaning; Control High-fat (CH), offspring of mothers GC, fed a hight-fat diet after weaning; High-fat Control (HC), offspring of mothers GH, fed with control diet after weaning; and High-fat High-fat (HH), offspring of mothers GH, fed a H diet after weaning. Results: HH and HC groups showed increased body weight compared to CC group and increases in caloric intake, larger amount of white adipose tissue and adipocyte size compared to CC and CH groups. The HH and CH groups showed higher NF-kB expression in white adipose tissue compared to the CC and HC groups, and the HH group also showed higher TNF- α expression. In the hypothalamus, the HH and HC groups exhibited higher TNF- α expression compared to the CC and CH groups. Conclusion: Perinatal and post-weaning exposure to the high-fat diet increases the amount of white adipose tissue, adipocyte size, and expression of the inflammatory genes TNF-α and NF-kB.
- Published
- 2022
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46. Leiomyoma and Leiomyosarcoma (Primary and Metastatic) of the Oral and Maxillofacial Region: A Clinicopathological and Immunohistochemical Study of 27 Cases.
- Author
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de Araújo GR, Costa SFDS, Mesquita RA, Gomez RS, Dos Santos JN, Pontes HAR, de Andrade BAB, Romañach MJ, Agostini M, Vargas PA, de Cáceres CVBL, Santos-Silva AR, Ribeiro ACP, Brandão TB, Tomasi RA, Ferreyra RS, de Almeida OP, and Fonseca FP
- Subjects
- Biomarkers, Tumor, Calmodulin-Binding Proteins, Female, Humans, Male, Middle Aged, Retrospective Studies, Leiomyoma diagnosis, Leiomyoma pathology, Leiomyosarcoma diagnosis, Leiomyosarcoma pathology, Smooth Muscle Tumor pathology, Uterine Neoplasms diagnosis, Uterine Neoplasms pathology
- Abstract
Smooth muscle neoplasms represent an important group of lesions which is rare in the oral cavity. Leiomyoma (LM) is benign smooth muscle/pericytic tumor usually presenting as non-aggressive neoplasm, while leiomyosarcoma (LMS) represents its malignant counterpart. The rarity of these lesions, together with its unspecific clinical presentation and a variable histopathological appearance, lead to a broad list of differential diagnoses, hampering their diagnoses. Therefore, in this study we describe the clinical and microscopic features of a series of oral and maxillofacial LMs and LMSs. A retrospective search from 2000 to 2019 was performed and all cases diagnosed as LM and LMS affecting the oral cavity and gnathic bones were retrieved. Clinical and demographic data were obtained from the patients' pathology records, while microscopic features and immunohistochemistry were reviewed and completed when necessary to confirm the diagnoses. Twenty-two LMs and five LMSs were obtained. In the LM group, males predominated, with a mean age of 45.7 years. The upper lip was the most affected site, and 18 cases were classified as angioleiomyomas and four as solid LM. In the LMS group, females predominated, with a mean age of 47.6 years. The mandible was the most affected site. Diffuse proliferation of spindle cells, with necrosis and mitotic figures, were frequent microscopic findings. LMs and LMSs were positive for α-smooth muscle actin, HHF-35 and h-caldesmon. In conclusion, oral LM/LMS are uncommon neoplasms with the latter usually presenting as metastatic disease. H&E evaluation may be very suggestive of oral LMs, but h-caldesmon staining is strongly recommended to confirm LMS diagnosis., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2022
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47. A new species of Rhabdias (Nematoda: Rhabdiasidae), a lung parasite of Pristimantis chiastonotus (Anura: Strabomantidae) from the Brazilian Amazon: description and phylogenetic analyses.
- Author
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Tavares-Costa LFS, Rebêlo GL, Müller MI, Jesus RF, Nandyara B, Silva LMO, Costa-Campos CE, Dos Santos JN, and Melo FTV
- Subjects
- Animals, Anura, Brazil, Lung, Phylogeny, Nematoda, Parasites
- Abstract
Rhabdias Stiles and Hassal, 1905 comprises approximately 90 species of parasitic lung nematodes of amphibians and reptiles that have a wide distribution, with 21 species occurring in the Neotropics. In the present study, we describe Rhabdias waiapi n. sp. found parasitizing the lungs of the anuran species Pristimantis chiastonotus from the Amazon Biome in the Amapá State, Northern Brazil. The new species is characterized by having an elongated body, expansions of the cuticular inflation in the anterior end that become more discrete along the body, an anterior end with a slight constriction at the level of the esophageal apex with four rounded subapical elevations of the body wall, six lips, four near the edge of the oral opening and two more distant lateral ones, and a gradually tapering elongated tail. In addition, molecular analyses and phylogenetic reconstructions were made, with sequences from the coding region of the mitochondrial cytochrome c oxidase subunit I gene. Those results strongly support the status of the new taxon, which formed a poorly supported clade with Rhabdias sp. 5 from Anolis brasiliensis from Northeast Brazil. Rhabdias waiapi n. sp. is the 19th species of the genus described in the Neotropics for amphibians, the 10th in Brazil, the second described from hosts of the family Strabomantidae from the Neotropical region, and the first amphibian nematode species described in the Amapá State., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2022
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48. Peripheral dentinogenic ghost cell tumor-report of two cases and review of the literature.
- Author
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da Silva Barros CC, de Souto Medeiros MR, de Azevedo RA, da Costa Miguel MC, Dos Santos JN, and da Silveira ÉJD
- Subjects
- Aged, Diagnosis, Differential, Female, Humans, Immunohistochemistry, Male, Middle Aged, Radiography, Panoramic, Ameloblastoma, Odontogenic Tumors diagnostic imaging, Odontogenic Tumors surgery
- Abstract
Peripheral dentinogenic ghost cell tumor (DGCT) is a rare and non-aggressive benign odontogenic tumor. They usually affect the elderly and are predominantly located in the anterior region of the jaws. Their differential diagnosis includes reactive/inflammatory gingival lesions. We report here two cases of peripheral DGCT in a 73-year-old female and a 48-year-old male patient and review the cases published in the literature. Both lesions presented as a nodular lesion in the mandible, and panoramic radiography showed no abnormalities. Microscopically, it was observed to be an ameloblastomatous epithelial proliferation associated with clusters of ghost cells and dysplastic dentin. Immunohistochemistry revealed positivity for cytokeratin 19 and a low Ki-67 proliferative index. Based on histopathological features and the absence of radiographic findings, a diagnosis of peripheral DGCT was rendered. The low number of cases published of peripheral DGCT makes case reports important in providing information that helps in their diagnoses and management., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
- Published
- 2021
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49. Antimicrobial resistance profile of extended-spectrum beta-lactamases, adenosine-monophosphate-cyclic, and carbapenemase-producing Gram-negative bacteria isolated from domestic animals.
- Author
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Dos Santos JP, de Souza VFM, Santos MWDC, Dos Santos JN, Dos Santos NS, de Oliveira AP, Rodrigues VTDS, Carneiro IO, and Vieira LCADS
- Abstract
Background and Aim: The production of beta-lactamase enzymes, such as extended-spectrum beta-lactamase (ESBL), adenosine-monophosphate-cyclic (AmpC), and Klebsiella pneumoniae carbapenemase (KPC), is one of the most important mechanisms of bacterial resistance to antimicrobials. Gram-negative bacteria show significant resistance due to various intrinsic and acquired factors. These intrinsic factors include low permeability of the outer membrane, various efflux systems, and the production of beta-lactamases, while acquired factors include chromosomal mutation and acquisition of resistance genes by horizontal transfer. Mobile elements such as plasmids, integrative conjugative elements, mobilizable islands, or transposable elements are involved in horizontal transfer. At present, the Gram-negative pathogens of most concern are Acinetobacter baumannii , Pseudomonas aeruginosa , and those belonging to the Enterobacteriaceae family (e.g., Escherichia coli , K. pneumoniae , and Proteus mirabilis ). This study aimed to evaluate the profile of antimicrobial resistance and the production of the enzymes ESBL, AmpC, and KPC, in 21 gram-negative bacteria isolated from domestic animals treated at the University Veterinary Hospital (HVU) of the Federal University of Western Bahia (UFOB)., Materials and Methods: The biological samples (21) were inoculated to brain heart infusion broth, blood agar, and MacConkey agar and incubated for 24-72 h at 37°C. Gram staining and identification through biochemical tests and matrix-associated laser desorption/ionization time-of-flight mass spectrometry were conducted. To evaluate the antimicrobial resistance profile, the disk diffusion method was used, and 25 antibiotics were employed. For the detection of ESBL, the disk approximation method was applied using chromogenic agar. The presence of KPC was observed using chromogenic agar and the Hodge test. For AmpC evaluation, the disk approximation method was used., Results: The most isolated agent was E. coli (66.66%, 14/21), followed by K. pneumoniae and P. mirabilis (both 14.29%, 3/21), and then Pasteurella spp. (4.76%, 1/21). The bacterial isolates showed high levels of resistance against clindamycin, penicillin, imipenem, polymyxin, cefoxitin, gentamycin, cefotaxime, ceftazidime, cephalothin, ceftriaxone, ciprofloxacin, trimethoprim/sulfamethoxazole, chloramphenicol, and tetracycline. The best effectiveness rates were observed for cefepime, streptomycin, amoxicillin-clavulanate, aztreonam, nalidixic acid, tobramycin, levofloxacin, amikacin, and meropenem. All biological isolates showed multiple resistance to at least three of the antibiotics tested (3/25), and some showed resistance to 24 of the antibiotics tested (24/25). Among the 21 pathogens analyzed, 8 were ESBL producers (38.09%); of these, 6 were identified as E. coli (28.57%), and 2 were identified as K. pneumoniae (9.52%). Two strains of K. pneumoniae produced both ESBL and KPC. None of the isolates were producers of AmpC., Conclusion: The results found in the present work raise concern about the level of antimicrobial resistance among pathogens isolated from domestic animals in Brazil. The results highlight the need for the development and implementation of anti-resistance strategies to avoid the dissemination of multiresistant pathogens, including the prudent use of antimicrobials and the implementation of bacterial culture, antimicrobial sensitivity, and phenotypic tests for the detection of beta-lactamase enzymes in bacteria isolated from animals., (Copyright: © dos Santos, et al.)
- Published
- 2021
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50. Evaluation of alveolar process resorption after tooth extraction using the socket shield technique without immediate installation of implants: a randomised controlled clinical trial.
- Author
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de Oliveira GB, Rebello IMC, Montanha Andrade K, Araujo NS, Dos Santos JN, and Cury PR
- Subjects
- Alveolar Process, Humans, Tooth Extraction, Tooth Socket, Alveolar Bone Loss, Dental Implants, Immediate Dental Implant Loading
- Abstract
Our aim was to evaluate resorption of the alveolar ridge using the socket shield technique (SST) without immediate placement of dental implants. This randomised controlled clinical trial included 27 patients: 14 maxillary non-molar teeth were partially extracted using the SST (test group) and 13 were extracted using a minimally traumatic extraction approach (control group). Alterations in height and thickness of the alveolar ridge were evaluated by cone beam computed tomograms taken immediately after, and 100 days after, surgery. Minor resorption was observed in the height of the buccal and palatal plates, without intergroup difference (p ≥ 0.10). The test group showed significantly better preservation of the buccal-to-palatal crest dimension (p ≤ 0.05). In the control group, preservation of buccal plate thickness was significantly greater (p ≤ 0.05), but intragroup vertical resorption of the buccal plate and reduction in the buccal-to-palatal crest distance were greater (p ≤ 0.05). The SST without the immediate placement of implants showed greater preservation of the buccal-to-palatal crest dimension and lower preservation of buccal wall thickness compared with minimally traumatic extraction. In addition, it provided superior maintenance of the baseline buccal wall height. The modified SST is a promising approach, but factors that interfere with the results should be investigated., (Copyright © 2021 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
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