1. Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage
- Author
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Michael S. Chin, Brian B. Freniere, Luca eLancerotto, Jorge R. Lujan-Hernandez, Jonathan H. Saleeby, Yuan Chyuan Lo, Dennis P. Orgill, Janice F. Lalikos, and Thomas J. Fitzgerald
- Subjects
Perfusion Imaging ,Radiation Dosage ,Radiation Effects ,hyperspectral imaging ,Radiation exposure ,diffuse reflectance imaging ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BACKGROUND: Radiation exposure can lead to detrimental effects in skin microcirculation. The precise relationship between radiation dose received and its effect on cutaneous perfusion still remains controversial. Previously, we have shown that hyperspectral imaging (HSI) is able to demonstrate long-term reductions in cutaneous perfusion secondary to chronic microvascular injury. This study characterizes the changes in skin microcirculation in response to varying doses of ionizing radiation and investigates these microcirculatory changes as a possible early non-invasive biomarker that may correlate with the extent of long-term microvascular damage.METHODS: Immunocompetent hairless mice (n=66) were exposed to single fractions of superficial beta-irradiation in doses of 0, 5, 10, 20, 35, or 50 Gy. A HSI device was utilized to measure deoxygenated hemoglobin levels in irradiated and control areas. HSI measurements were performed at baseline before radiation exposure and for the first three days post-irradiation. Maximum macroscopic skin reactions were graded, and histological assessment of cutaneous microvascular densities at four weeks post-irradiation was performed in harvested tissue by CD31 immunohistochemistry.RESULTS: CD31 immunohistochemistry demonstrated a significant correlation (r=0.90, p
- Published
- 2015
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