1. Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis.
- Author
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Dos Santos Jaques JA, Becker LV, Souza Vdo C, Leal CA, Bertoldo TM, de Vargas Pinheiro K, Morsch VM, Schetinger MR, and Leal DB
- Subjects
- Adenosine Diphosphate metabolism, Adenosine Triphosphate metabolism, Adult, Arthritis, Rheumatoid pathology, Case-Control Studies, Cells, Cultured, Enzyme Assays, Female, Humans, Lymphocytes pathology, Male, Middle Aged, Adenosine Deaminase metabolism, Antigens, CD metabolism, Apyrase metabolism, Arthritis, Rheumatoid enzymology, Lymphocytes enzymology
- Abstract
The purpose of this study was to investigate the activities of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and adenosine deaminase (E-ADA; EC 3.5.4.4) in lymphocytes from patients with rheumatoid arthritis (RA). Thirty patients diagnosed with RA through American College of Rheumatology criteria as well as 30 healthy patients were selected. Peripheral blood lymphocytes were isolated, and E-NTPDase and E-ADA activities were assayed. The results demonstrated an increased E-NTPDase activity (both ATP and ADP as substrates) and a decreased E-ADA activity in RA patients. These data suggest an organic effort to preserve the adenosine level, which is known to have anti-inflammatory and analgesic properties, working as a potent suppressor of immune response., (Copyright © 2012 John Wiley & Sons, Ltd.)
- Published
- 2013
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