Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis.

Authors :: Dos Santos Jaques JA
Becker LV
Souza Vdo C
Leal CA
Bertoldo TM
de Vargas Pinheiro K
Morsch VM
Schetinger MR
Leal DB
Source :: Cell biochemistry and function [Cell Biochem Funct] 2013 Jul; Vol. 31 (5), pp. 395-9. Date of Electronic Publication: 2012 Oct 16.
Publication Year :: 2013
Abstract: The purpose of this study was to investigate the activities of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and adenosine deaminase (E-ADA; EC 3.5.4.4) in lymphocytes from patients with rheumatoid arthritis (RA). Thirty patients diagnosed with RA through American College of Rheumatology criteria as well as 30 healthy patients were selected. Peripheral blood lymphocytes were isolated, and E-NTPDase and E-ADA activities were assayed. The results demonstrated an increased E-NTPDase activity (both ATP and ADP as substrates) and a decreased E-ADA activity in RA patients. These data suggest an organic effort to preserve the adenosine level, which is known to have anti-inflammatory and analgesic properties, working as a potent suppressor of immune response.<br /> (Copyright © 2012 John Wiley & Sons, Ltd.)

Subjects :: Adenosine Diphosphate metabolism
Adenosine Triphosphate metabolism
Adult
Arthritis, Rheumatoid pathology
Case-Control Studies
Cells, Cultured
Enzyme Assays
Female
Humans
Lymphocytes pathology
Male
Middle Aged
Adenosine Deaminase metabolism
Antigens, CD metabolism
Apyrase metabolism
Arthritis, Rheumatoid enzymology
Lymphocytes enzymology

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