35 results on '"de Oliveira CH"'
Search Results
2. Terbinafine quantification in human plasma by high-performance liquid chromatography coupled to electrospray tandem mass spectrometry: application to a bioequivalence study
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de Moraes Mo, de Nucci G, Barrientos-Astigarraga Re, Bezerra Fa, de Moraes Me, and de Oliveira Ch
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Pharmacology ,Detection limit ,Spectrometry, Mass, Electrospray Ionization ,Chromatography ,Antifungal Agents ,Chemistry ,Electrospray ionization ,Bioequivalence ,Naphthalenes ,Tandem mass spectrometry ,High-performance liquid chromatography ,Crossover study ,Pharmacokinetics ,Therapeutic Equivalency ,medicine ,Terbinafine ,Humans ,Pharmacology (medical) ,Chromatography, High Pressure Liquid ,medicine.drug - Abstract
A method based on liquid chromatography with positive ion electrospray ionization and tandem mass spectrometry is described for the determination of terbinafine in human plasma using naftifine as internal standard. The method has a chromatographic run time of 5 minutes and was linear in the range 1.0 to 2000 ng/mL. The limit of quantification was 1.0 ng/mL; the intraday precision was 3.6%, 3.8%, 3.5%, and 4.1%; and the intraday accuracy was -2.7%, 7.7%, 4.8%, and -2.7% for 5.0, 80.0, 250.0, and 1500.0 ng/mL, respectively. The interday precision was 4.9%, 1.7%, 2.4%, and 4.6% and the interday accuracy was 0.3%, 5.8%, 6.5%, and -1.4% for the same concentrations. This method was used in a bioequivalence study of two tablet formulations of terbinafine. Twenty-four healthy volunteers (both sexes) received a single oral dose of terbinafine (250 mg) in an open, randomized, two-period crossover study. The 90% CI of geometric mean ratios between Terbinafina (Medley S/A Industria Farmaceutica, Campinas, Brazil) and Lamisil (Novartis Biociencias S/A, Sao Paulo, Brazil) were 90.5% to 110.0% for C max, 92.2% to 108.1% for AUC last, and 91.3% to 107.5% for AUC 0-inf. Because the 90% CI for the above-mentioned parameters were included in the 80% to 125% interval proposed by the US FDA, the two formulations were considered bioequivalent in terms of rate and extent of absorption.
- Published
- 2002
3. Challenges to develop risk assessment schemes for Brazilian bees: multiple exposure routes
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Nocelli, Roberta C. F., de Oliveira Cham, Karina, and Malaspina, Osmar
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Agriculture ,Botany ,QK1-989 - Published
- 2018
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4. Characterization of Aeromonas Isolates from Ornamental Fish: Species, Virulence Genes, and Antimicrobial Susceptibility.
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de Oliveira CH, Moreno LZ, Cardoso PHM, Silva APS, Gomes VTM, Barbosa MRF, Balian SC, and Moreno AM
- Abstract
This study aimed to characterize 300 Aeromonas spp. strains isolated from 123 ornamental fish of 32 different species presenting with septicemia, skin lesions, and/or eye lesions. Within the 300 strains, 53.0% were identified as A. veronii , 41.3% as A. hydrophila , and 5.7% as A. caviae . Among the six virulence genes investigated, the most frequent were act (90.3%) and aer (79.3%). More than 50% of A. hydrophila strains were positive for all the studied genes. A total of 30 virulence profiles were identified, with the five main profiles identified comprising 75% of strains. Only five strains were negative for all genes and were identified as A. caviae and A. veronii . The antimicrobial susceptibility profile was performed for 234 strains, with sulfonamides presenting more than 50% of the resistance rates. Susceptibility was observed mainly for cephalosporins, aminoglycosides, chloramphenicol and piperacillin-tazobactam. Multidrug resistance was detected in 82.5% of the studied strains, including A. caviae with 100% multidrug resistance, and A. hydrophila with 90.9% multidrug resistance. The SE-AFLP analysis resulted in 66 genotypes of A. hydrophila , 118 genotypes of A. veronii , and 14 genotypes of A. caviae , demonstrating the greater heterogeneity of A. veronii and A. caviae . However, no direct correlation was observed between the genotypes and the strains' origins or virulence and resistance profiles.
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- 2024
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5. Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021.
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de Oliveira CH, Andrade MS, Campos FS, da C Cardoso J, Gonçalves-Dos-Santos ME, Oliveira RS, Aquino-Teixeira SM, Campos AA, Almeida MA, Simonini-Teixeira D, da P Sevá A, Temponi AOD, Magalhães FM, da Silva Menezes AS, Lopes BT, Almeida HP, Pedroso AL, Gonçalves GP, Chaves DCC, de Menezes GG, Bernal-Valle S, Müller NF, Janssen L, Dos Santos E, Mares-Guia MA, Albuquerque GR, Romano AP, Franco AC, Ribeiro BM, Roehe PM, Lourenço-de-Oliveira R, and de Abreu FVS
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- Humans, Animals, Seasons, Brazil epidemiology, Mosquito Vectors, Yellow fever virus genetics, Culicidae
- Abstract
In recent decades, waves of yellow fever virus (YFV) from the Amazon Rainforest have spread and caused outbreaks in other regions of Brazil, including the Cerrado, a savannah-like biome through which YFV usually moves before arriving at the Atlantic Forest. To identify the vectors involved in the maintenance of the virus in semiarid environments, an entomological survey was conducted after confirmation of yellow fever (YF) epizootics at the peak of the dry season in the Cerrado areas of the state of Minas Gerais. In total, 917 mosquitoes from 13 taxa were collected and tested for the presence of YFV. Interestingly, mosquitoes of the Sabethes genus represented 95% of the diurnal captured specimens, displaying a peak of biting activity never previously recorded, between 4:30 and 5:30 p.m. Molecular analysis identified three YFV-positive pools, two from Sabethes chloropterus -from which near-complete genomes were generated-and one from Sa. albiprivus , whose low viral load prevented sequencing. Sa. chloropterus was considered the primary vector due to the high number of copies of YFV RNA and the high relative abundance detected. Its bionomic characteristics allow its survival in dry places and dry time periods. For the first time in Brazil, Sa. albiprivus was found to be naturally infected with YFV and may have played a role as a secondary vector. Despite its high relative abundance, fewer copies of viral RNA were found, as well as a lower Minimum Infection Rate (MIR). Genomic and phylogeographic analysis showed that the virus clustered in the sub-lineage YFV
PA-MG , which circulated in Pará in 2017 and then spread into other regions of the country. The results reported here contribute to the understanding of the epidemiology and mechanisms of YFV dispersion and maintenance, especially in adverse weather conditions. The intense viral circulation, even outside the seasonal period, increases the importance of surveillance and YFV vaccination to protect human populations in affected areas.- Published
- 2023
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6. MGAT2 inhibitor decreases liver fibrosis and inflammation in murine NASH models and reduces body weight in human adults with obesity.
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Cheng D, Zinker BA, Luo Y, Shipkova P, De Oliveira CH, Krishna G, Brown EA, Boehm SL, Tirucherai GS, Gu H, Ma Z, Chu CH, Onorato JM, Kopcho LM, Ammar R, Smith J, Devasthale P, Lawrence RM, Stryker SA, Dierks EA, Azzara AV, Carayannopoulos L, Charles ED, Lentz KA, and Gordon DA
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- Animals, Humans, Mice, Body Weight, Inflammation drug therapy, Liver Cirrhosis drug therapy, Adult, Clinical Trials, Phase I as Topic, Non-alcoholic Fatty Liver Disease drug therapy, Obesity drug therapy
- Abstract
Monoacylglycerol acyltransferase 2 (MGAT2) is an important enzyme highly expressed in the human small intestine and liver for the regulation of triglyceride absorption and homeostasis. We report that treatment with BMS-963272, a potent and selective MGAT2 inhibitor, decreased inflammation and fibrosis in CDAHFD and STAM, two murine nonalcoholic steatohepatitis (NASH) models. In high-fat-diet-treated cynomolgus monkeys, in contrast to a selective diacylglycerol acyltransferase 1 (DGAT1) inhibitor, BMS-963272 did not cause diarrhea. In a Phase 1 multiple-dose trial of healthy human adults with obesity (NCT04116632), BMS-963272 was safe and well tolerated with no treatment discontinuations due to adverse events. Consistent with the findings in rodent models, BMS-963272 elevated plasma long-chain dicarboxylic acid, indicating robust pharmacodynamic biomarker modulation; increased gut hormones GLP-1 and PYY; and decreased body weight in human subjects. These data suggest MGAT2 inhibition is a promising therapeutic opportunity for NASH, a disease with high unmet medical needs., Competing Interests: Declaration of interests A.V.A., R.A., and S.A.S. were employees of Bristol Myers Squibb when the studies were conducted. All other authors are current employees of Bristol Myers Squibb and have Bristol Myers Squibb stock., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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7. Effects of incubation temperature on development, morphology, and thermal physiology of the emerging Neotropical lizard model organism Tropidurus torquatus.
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De-Lima AKS, de Oliveira CH, Pic-Taylor A, and Klaczko J
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- Animals, Climate Change, Ecosystem, Female, Global Warming, Temperature, Lizards physiology
- Abstract
Incubation temperature is among the main phenotypic trait variation drivers studied since the developmental trajectory of oviparous animals is directly affected by environmental conditions. In the last decades, global warming predictions have aroused interest in understanding its impacts on biodiversity. It is predicted that the effects of direct warming will be exacerbated by other anthropogenic factors, such as microclimatic edge effects. Although the Brazilian Cerrado biome is one of the most affected by these issues, little is known about the aforementioned effects on its biodiversity. Therefore, the aim of our study is to investigate the influence of incubation temperature on developmental parameters, morphology and thermal physiology traits of the collared lizard (Tropidurus torquatus). Furthermore, we discuss our findings regarding lizard developmental biology and the climate change paradigm. Therefore, we incubated T. torquatus eggs under five temperature regimes ranging from artificial nest temperature (28.7 °C) to 35.0 °C. We found that elevated incubation temperatures affect several investigated traits: egg mass gain is positively affected, without any influence in newborn mass; incubation period is broadly reduced with temperature increase; survival rate is negatively affected by temperature, constant 35.0 °C regime is confirmed as a lethal incubation temperature, and the sex ratio is affected at 30.0 °C, with a prevailing outbreak of females. Increased incubation temperature also affects body and head size but has no effect on limb size. Newborn thermoregulation and the critical thermal maximum (CT
max ) are not affected by incubation temperature. On the other hand, basal body temperature (Tbb ) and the critical thermal minimum (CTmin ) were positively affected. Thermal physiology was also affected by age, with newborns differing from adults for all analyzed thermal traits. Our findings indicate that future modifications in incubation temperature regimes at nesting sites caused by warming may affect several features of the development, morphology, and thermal physiology of newborns of this species. Laboratory experiments have pointed to possible drastic effects of warming on lizard survival rates, also affecting aspects of its natural history and population distribution. Moreover, in addition to being more vulnerable than adults in aspects such as predation and feeding, T. torquatus newborns are also more vulnerable regarding thermal physiological traits., (© 2022. The Author(s).)- Published
- 2022
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8. Serological Evidence of Orthopoxvirus Infection in Neotropical Primates in Brazil.
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Abreu FVS, Lorene Soares Rocha K, Silva-Oliveira R, Macedo MV, Silva TGM, Gonçalves-Dos-Santos ME, de Oliveira CH, Aquino-Teixeira SM, Ottone VO, da Silva AJJ, Dos Santos RM, Tátila-Ferreira A, Almeida MAB, Dos Santos E, da Cruz Cardoso J, Campos AAS, Albuquerque GR, da Paixão Sevá A, Ribeiro BM, Simonini Teixeira D, Campos FS, Franco AC, Roehe PM, de Souza Trindade G, and Bretas de Oliveira D
- Abstract
The genus Orthopoxvirus (OPXV) of the family Poxviridae comprises several viruses that are capable of infecting a wide range of hosts. One of the most widespread OPXVs is the Vaccinia virus (VACV), which circulates in zoonotic cycles in South America, especially in Brazil, infecting domestic and wild animals and humans and causing economic losses as well as impacting public health. Despite this, little is known about the presence and/or exposure of neotropical primates to orthopoxviruses in the country. In this study, we report the results of a search for evidence of OPVX infections in neotropical free-living primates in the state of Minas Gerais, southeast Brazil. The sera or liver tissues of 63 neotropical primates were examined through plaque reduction neutralization tests (PRNT) and real-time PCR. OPXV-specific neutralizing antibodies were detected in two sera (4.5%) from Callithrix penicillata , showing 55% and 85% reduction in plaque counts, evidencing their previous exposure to the virus. Both individuals were collected in urban areas. All real-time PCR assays were negative. This is the first time that evidence of OPXV exposure has been detected in C. penicillata , a species that usually lives at the interface between cities and forests, increasing risks of zoonotic transmissions through spillover/spillback events. In this way, studies on the circulation of OPXV in neotropical free-living primates are necessary, especially now, with the monkeypox virus being detected in new regions of the planet.
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- 2022
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9. No Evidence of SARS-CoV-2 Infection in Neotropical Primates Sampled During COVID-19 Pandemic in Minas Gerais and Rio Grande do Sul, Brazil.
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de Abreu FVS, Macedo MV, da Silva AJJ, de Oliveira CH, de Ottone VO, de Almeida MAB, Dos Santos E, da Cardoso JC, Campos AS, da Silva CMD, da Silva AG, de Andrade MS, Bernis VMO, Bernis Filho WO, de Trindade GS, Albuquerque GR, da Sevá AP, Ribeiro BM, Teixeira DS, Campos FS, Franco AC, Roehe PM, and de Oliveira DB
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- Animals, Brazil, Humans, Primates, SARS-CoV-2, COVID-19, Pandemics
- Abstract
In 2019, a new coronavirus disease (COVID-19) was detected in China. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was capable to infect domestic and captive mammals like cats, tigers and minks. Due to genetic similarities, concern about the infection of non-human primates (NHPs) and the establishment of a sylvatic cycle has grown in the Americas. In this study, neotropical primates (NP) were sampled in different areas from Brazil to investigate whether they were infected by SARS-CoV-2. A total of 89 samples from 51 NP of four species were examined. No positive samples were detected via RT-qPCR, regardless of the NHP species, tissue or habitat tested. This work provides the first report on the lack of evidence of the circulation of SARS-CoV-2 in NP. The expansion of wild animals sampling is necessary to understand their role in the epidemiology of SARS-CoV-2 and other potentially zoonotic pathogens in natural environments shared by humans., (© 2021. EcoHealth Alliance.)
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- 2021
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10. The impact of fake news on social media and its influence on health during the COVID-19 pandemic: a systematic review.
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Rocha YM, de Moura GA, Desidério GA, de Oliveira CH, Lourenço FD, and de Figueiredo Nicolete LD
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Purpose: As the new coronavirus disease propagated around the world, the rapid spread of news caused uncertainty in the population. False news has taken over social media, becoming part of life for many people. Thus, this study aimed to evaluate, through a systematic review, the impact of social media on the dissemination of infodemic knowing and its impacts on health., Methods: A systematic search was performed in the MedLine, Virtual Health Library (VHL), and Scielo databases from January 1, 2020, to May 11, 2021. Studies that addressed the impact of fake news on patients and healthcare professionals around the world were included. It was possible to methodologically assess the quality of the selected studies using the Loney and Newcastle-Ottawa Scales., Results: Fourteen studies were eligible for inclusion, consisting of six cross-sectional and eight descriptive observational studies. Through questionnaires, five studies included measures of anxiety or psychological distress caused by misinformation; another seven assessed feeling fear, uncertainty, and panic, in addition to attacks on health professionals and people of Asian origin., Conclusion: By analyzing the phenomenon of fake news in health, it was possible to observe that infodemic knowledge can cause psychological disorders and panic, fear, depression, and fatigue., Competing Interests: Conflict of interestThe authors declare no conflict of interest., (© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.)
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- 2021
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11. Main bacterial species causing clinical disease in ornamental freshwater fish in Brazil.
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Cardoso PHM, Moreno LZ, de Oliveira CH, Gomes VTM, Silva APS, Barbosa MRF, Sato MIZ, Balian SC, and Moreno AM
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- Animals, Brazil, Fishes, Fresh Water, Humans, Aeromonas, Fish Diseases
- Abstract
Bacterial diseases are common in ornamental fish, more frequently associated with ubiquitous bacteria from the aquarium environment. The disease can lead to fish mortality and cause high economic losses if not rapidly controlled. The aim of this study was to identify the main causative bacterial agents of infection in ornamental fish with different clinical signs. A total of 126 freshwater fish, from 12 families and 38 species, with clinical signs were collected in a wholesaler in São Paulo, SP, Brazil. Samples were taken from the eye, skin ulcers, kidneys, and gills, plated on MacConkey, CHROMagar Orientation, and blood agar and incubated under aerobic and anaerobic conditions. Bacterial identification was performed by MALDI-TOF mass spectrometry. From the 126 studied animals, 112 were positive for bacterial isolation. Among the positive animals, 32.1% presented infection caused by a single bacterial species, while in the remaining 67.9%, two to six different bacterial species were identified. A total of 259 bacterial strains were obtained and classified among 46 bacterial species. The species of higher frequency were Aeromonas veronii (26.3%), Aeromonas hydrophilla (16.2%), Shewanella putrefaciens (7.3%), Citrobacter freundii (8.1%), Vibrio albensis (5.8%), and Klebsiella pneumoniae (4.2%). MALDI-TOF MS showed to be a rapid method for diagnosis of bacterial disease outbreaks in ornamental fish establishments.
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- 2021
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12. Studies on the interaction between HSA and new halogenated metformin derivatives: influence of lipophilic groups in the binding ability.
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Chaves OA, Mathew B, Parambi DGT, C S de Oliveira CH, Cesarin-Sobrinho D, Lakshminarayanan B, Najeeb S, Nafna EK, Marathakam A, Uddin MS, Joy M, and Carlos Netto-Ferreira J
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- Binding Sites, Circular Dichroism, Humans, Molecular Docking Simulation, Protein Binding, Serum Albumin, Human metabolism, Spectrometry, Fluorescence, Thermodynamics, Diabetes Mellitus, Type 2, Metformin
- Abstract
In the type II diabetes mellitus, Metformin hydrochloride is recommended as a common FAD approved drug. Synthesis of novel metformin series has been widely explored, mainly due to its biological importance and to improve their pharmacokinetic profile. Generally, human serum albumin (HSA) is the main protein used to study drug viability in vitro analysis. Thus, the present study reports the synthesis of three new halogenated metformin derivatives (MFCl, MFBr and MFCF
3 ) and its interaction toward HSA by multiple spectroscopic techniques (UV-Vis, circular dichroism, steady-state, time-resolved and synchronous fluorescence), combined to computational methods (molecular docking and quantum chemical calculation). The interaction between each halogenated metformin derivative and HSA is spontaneous ( ΔG °<0), entropically driven ( ΔS °>0), moderate ( Ka and Kb ≈ 104 M-1 ) and occurs preferentially in the subdomain IIA (close to Trp-214 residue). Molecular docking results suggested hydrogen bonding, van der Waals and hydrophobic interactions as the main binding forces. Quantum chemical calculations suggested imino groups as the most intense electrostatic negative potentials, while the positive electrostatic potential is located at the hydrogen atoms on N , N -dimethyl and the phenyl systems which can help the hydrophobic interactions. [Formula: see text]Communicated by Ramaswamy H. Sarma.- Published
- 2020
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13. Slow slip source characterized by lithological and geometric heterogeneity.
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Barnes PM, Wallace LM, Saffer DM, Bell RE, Underwood MB, Fagereng A, Meneghini F, Savage HM, Rabinowitz HS, Morgan JK, Kitajima H, Kutterolf S, Hashimoto Y, Engelmann de Oliveira CH, Noda A, Crundwell MP, Shepherd CL, Woodhouse AD, Harris RN, Wang M, Henrys S, Barker DHN, Petronotis KE, Bourlange SM, Clennell MB, Cook AE, Dugan BE, Elger J, Fulton PM, Gamboa D, Greve A, Han S, Hüpers A, Ikari MJ, Ito Y, Kim GY, Koge H, Lee H, Li X, Luo M, Malie PR, Moore GF, Mountjoy JJ, McNamara DD, Paganoni M, Screaton EJ, Shankar U, Shreedharan S, Solomon EA, Wang X, Wu HY, Pecher IA, and LeVay LJ
- Abstract
Slow slip events (SSEs) accommodate a significant proportion of tectonic plate motion at subduction zones, yet little is known about the faults that actually host them. The shallow depth (<2 km) of well-documented SSEs at the Hikurangi subduction zone offshore New Zealand offers a unique opportunity to link geophysical imaging of the subduction zone with direct access to incoming material that represents the megathrust fault rocks hosting slow slip. Two recent International Ocean Discovery Program Expeditions sampled this incoming material before it is entrained immediately down-dip along the shallow plate interface. Drilling results, tied to regional seismic reflection images, reveal heterogeneous lithologies with highly variable physical properties entering the SSE source region. These observations suggest that SSEs and associated slow earthquake phenomena are promoted by lithological, mechanical, and frictional heterogeneity within the fault zone, enhanced by geometric complexity associated with subduction of rough crust., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
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- 2020
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14. First report of mcr-1-harboring Salmonella enterica serovar Schwarzengrund isolated from poultry meat in Brazil.
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Moreno LZ, Gomes VTM, Moreira J, de Oliveira CH, Peres BP, Silva APS, Thakur S, La Ragione RM, and Moreno AM
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- Animals, Anti-Bacterial Agents pharmacology, Brazil, Colistin pharmacology, Genotype, Microbial Sensitivity Tests, Multilocus Sequence Typing, Poultry, Salmonella enterica genetics, Serogroup, Whole Genome Sequencing, Drug Resistance, Bacterial, Escherichia coli Proteins genetics, Meat microbiology, Plasmids analysis, Salmonella enterica drug effects, Salmonella enterica isolation & purification
- Abstract
Brazilian poultry meat samples were screened for colistin-resistant Salmonella enterica. Sixty Salmonella isolates were tested for in vitro colistin resistance and mcr-1, mcr-2, mcr-3 and mcr-4 genes. Two isolates harbored the mcr-1 gene and whole-genome analysis determined the serovar to be Schwarzengrund, ST96, harboring the IncX4 plasmid. This is the first report of mcr-1-harboring Salmonella enterica serovar Schwarzengrund in Brazil., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2019
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15. Phenotypic, molecular and genomic characterization of Actinobaculum suis isolated from swine in Brazil.
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Amigo CR, Moreno LZ, Ferreira TSP, Gomes VTM, Poor AP, de Oliveira CH, Parra BM, Silva APS, and Moreno AM
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- Actinomycetaceae classification, Actinomycetaceae physiology, Actinomycetales Infections microbiology, Amplified Fragment Length Polymorphism Analysis, Animals, Anti-Bacterial Agents pharmacology, Brazil, Drug Resistance, Bacterial, Electrophoresis, Gel, Pulsed-Field, Genomics, Microbial Sensitivity Tests, Molecular Typing, Polymerase Chain Reaction, Swine, Whole Genome Sequencing, Actinomycetaceae genetics, Actinomycetaceae isolation & purification, Actinomycetales Infections veterinary, Genotype, Phenotype, Swine Diseases microbiology
- Abstract
Urinary tract infections (UTI) are considered one of the most important diseases of sows due to its close relationship with reproductive problems such as reduced litter size, increase in the rate of return to estrous, vulvar discharge, abortion, mastitis and anestrus. Actinobaculum suis is one of the main agents involved in porcine urinary tract infection and is responsible for the most severe and fatal cases in sows. In the present report, 23 A. suis strains isolated from a sow and boars in Brazil were identified by PCR and further characterized by broth microdilution, molecular typing by pulsed-field gel electrophoresis (PFGE), single-enzyme amplified fragment length polymorphism (SE-AFLP), and whole-genome sequencing. All strains were sensitive to ceftiofur, linezolid, nitrofurantoin, quinupristin-dalfopristin and vancomycin. Ciprofloxacin, daptomycin, lincomycin, erythromycin and tylosin resistance was observed in 100% of tested strains. Tetracycline and tigecycline also presented high resistance rates (87% and 30.4%, respectively). PFGE with eight different restriction enzymes and three programs did not enable strain characterization; however, all strains were typed by SE-AFLP that clustered strains according to their origin, thus proving an effective tool for A. suis genotyping. Whole-genome sequencing and comparative analysis enabled species differentiation from closely related genus. This is the first report of genomic characterization of A. suis., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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16. Genetic diversity, virulence genotype and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from sows.
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Spindola MG, Cunha MPV, Moreno LZ, Amigo CR, Silva APS, Parra BM, Poor AP, de Oliveira CH, Perez BP, Knöbl T, and Moreno AM
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- Animals, Anti-Bacterial Agents therapeutic use, Brazil, Drug Resistance, Multiple, Bacterial, Escherichia coli Infections drug therapy, Escherichia coli Infections microbiology, Escherichia coli Infections urine, Female, Genetic Variation, Genotype, Swine, Swine Diseases drug therapy, Swine Diseases urine, Urinary Tract Infections microbiology, Urinary Tract Infections urine, Virulence, Escherichia coli Infections veterinary, Swine Diseases microbiology, Urinary Tract Infections veterinary, Uropathogenic Escherichia coli drug effects, Uropathogenic Escherichia coli genetics, Uropathogenic Escherichia coli isolation & purification
- Abstract
Background: Urinary tract infections (UTI) cause severe losses to the swine industry worldwide and uropathogenic Escherichia coli (UPEC) are the main agent isolated from UTI in sows., Objective: The aim of this study was to investigate the virulence genes, assess the phylogenetic background, clonal diversity, and the pattern of resistance to antimicrobials in 186 isolates of UPEC isolated from sows in Brazil., Materials and Methods: Urine samples from 300 sows of three herds with clinical signs from São Paulo State (Brazil) were screened for UTI; samples with suggestive results were submitted to bacterial isolation. E. coli strains isolated were characterized using disk diffusion technique, polymerase chain reaction and Single-enzyme amplification fragment length polymorphism (SE-AFLP)., Results: Virulence genes focH and papC were present in 78.5% and 58% of strains, respectively, followed by cnf1 (23.2%), afa (13.4%), sfa (11.3%), iucD (6.9%), and hlyA (1.6%). No clonal relatedness was found by SE-AFLP. A total of 98% of isolates (182/186) were multidrug resistant, and the highest levels of resistance were to sulfonamides, tetracycline, florfenicol, and ampicillin. Isolates were classified in phylogenetic group B1 (34.4%), followed by D (33.9%), E (30.1%) and A (1.6%)., Conclusions: The data obtained suggest that pigs from clinically affected herds may serve as a reservoir of uropathogenic and multidrug-resistant E. coli strains.
- Published
- 2018
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17. Draft Genome Sequence of Brazilian Leptospira interrogans Serovar Pomona Strain GR5, Isolated from Apparently Healthy Gilt.
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Moreno LZ, Miraglia F, de Oliveira CH, Vasconcellos SA, Heinemann MB, and Moreno AM
- Abstract
Leptospira interrogans serovar Pomona is one of the most important serovars associated with worldwide porcine leptospirosis, and its infection is characterized by high antibody titers and the establishment of a renal carrier state. Here, we present the draft genome sequence of Leptospira interrogans serovar Pomona strain GR5 isolated from apparently healthy gilt in Brazil., (Copyright © 2018 Moreno et al.)
- Published
- 2018
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18. Primary undifferentiated sarcoma in the thorax: a rare diagnosis in young patients.
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Waszczynskyi CH, Guimarães MD, Franco LF, Hochhegger B, and Marchiori E
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- 2016
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19. Multicentric lymphoma in buffaloes in the Amazon region, Brazil.
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De Oliveira CH, Barbosa JD, Damasceno KA, Cassali GD, Oliveira CM, Leite RC, and Reis JK
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- Animals, B-Lymphocytes cytology, B-Lymphocytes pathology, Brazil, Cell Proliferation, Female, Immunodeficiency Virus, Bovine genetics, Immunodeficiency Virus, Bovine isolation & purification, Lymphoma diagnosis, Lymphoma pathology, Lymphoma virology, Male, Buffaloes, Lymphoma veterinary
- Abstract
Background: The presence of lymphoma in buffaloes was first reported in India in the 1960s. The disease is similar to Enzootic Bovine Leucosis (EBL) caused by Bovine leukemia virus (BLV) in cattle; however, according to our results and those of other studies, the etiology of these lymphomas in buffalo do not appear to be associated with BLV. The objectives of this study are to describe four cases of the disease in buffaloes belonging to the same herd in the Amazon region of Brazil and to perform a clinical-anatomopathological, immunohistochemical, and etiological study of the lymphomas., Results: Over a period of ten years, four buffaloes were observed presenting progressive weight loss, swelling of peripheral lymph nodes, and nodules in the subcutaneous tissue. Upon necropsy, whitish-colored tumor masses were observed in the form of nodules in the subcutaneous tissue, along with miliary nodules on the serosal surfaces of abdominal and thoracic organs and tumors in lymph nodes and other organs. Neoplastic lymphocyte proliferation was observed through histopathology. An immunohistochemical study revealed that the neoplasias were formed by proliferation of predominantly B lymphocytes. The presence of BLV genome was not detected in the lymphomas when using the real-time PCR technique, nor was it detected through immunohistochemical staining using monoclonal antibodies against two viral proteins. Bovine herpesvirus 6 was not detected in the tumors. However, Bovine immunodeficiency virus (BIV) was detected in samples of lymphoma and in the lymph nodes and kidneys of one of the animals., Conclusions: The occurrence of lymphoma in buffaloes is reported for the first time in Brazil and is characterized by B-cell multicentric lymphoma. The etiology of the disease does not appear to be associated with BLV; however, the detection of BIV in samples of lymphoma from one sick animal deserves further study, considering the oncogenic potential of this virus.
- Published
- 2016
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20. Absence of Bovine leukemia virus (BLV) infection in buffaloes from Amazon and southeast region in Brazil.
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De Oliveira CH, Resende CF, Oliveira CM, Barbosa JD, Fonseca AA Junior, Leite RC, and Reis JK
- Subjects
- Animals, Antibodies, Viral blood, Brazil, Cattle, DNA, Viral blood, Enzootic Bovine Leukosis blood, Enzyme-Linked Immunosorbent Assay veterinary, False Negative Reactions, Genes, env, Genes, pX, Immunodiffusion methods, Lymphoma etiology, Lymphoma veterinary, Polymerase Chain Reaction veterinary, Buffaloes, Enzootic Bovine Leukosis diagnosis, Leukemia Virus, Bovine immunology, Leukemia Virus, Bovine isolation & purification
- Abstract
Enzootic bovine leucosis is an infectious disease caused by Bovine leukemia virus (BLV) and is well described in bovines. The majority of infected animals are asymptomatic, one to five percent develop lymphoma and from 30 to 50% present a persistent lymphocytosis. The virus occurs naturally in cattle and experimentally in buffaloes, capybaras and rabbits. The occurrence of lymphoma in buffaloes has been attributed to BLV infection by some authors in India and Venezuela, but not confirmed by other studies and little information on natural BLV infection in buffaloes is available. The aim of this study was to evaluate the occurrence of BLV in a sub-sample of buffalo from Amazon and southeast regions in Brazil. Three hundred and fifteen serum samples were negative using commercial AGID and ELISA (ELISA-gp51) which detect anti-BLV glycoprotein gp51 antibodies. The same samples were also evaluated for antibodies to whole virus through a commercial ELISA (ELISA-BLV) in which 77 (24.44%) were found seropositive and two (0.63%) inconclusive. On the other hand, all animals were negative by PCR to BLV targeted to the env and tax genes. These results suggest that ELISA-BLV produces false positive results in buffalo serum (p<0.001). In addition, one buffalo lymphoma sample was negative in both PCR assays used in this study. BLV was not detected in buffaloes from the Amazon basin and the southeast region of Brazil. Serological tests, like ELISA-BLV, usually used for cattle may produce false-positive results for BLV in buffaloes and direct detection tests such as PCR should be chosen in these surveys. The occurrence of lymphoma in buffalo was not associated with BLV infection in the one case analyzed in this work and the etiology and pathogenesis of this disease should be clarified., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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21. The detection of Vaccinia virus confirms the high circulation of Orthopoxvirus in buffaloes living in geographical isolation, Marajó Island, Brazilian Amazon.
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Franco-Luiz AP, Fagundes Pereira A, de Oliveira CH, Barbosa JD, Oliveira DB, Bonjardim CA, Ferreira PC, de Souza Trindade G, Abrahão JS, and Kroon EG
- Subjects
- Animals, Brazil epidemiology, Disease Outbreaks veterinary, Geography, Orthopoxvirus genetics, Orthopoxvirus isolation & purification, Phylogeny, Poxviridae Infections virology, Sequence Alignment, Seroepidemiologic Studies, Vaccinia epidemiology, Vaccinia virology, Zoonoses, Antibodies, Viral blood, Buffaloes virology, Orthopoxvirus immunology, Poxviridae Infections veterinary, Vaccinia veterinary, Vaccinia virus immunology, Vaccinia virus isolation & purification
- Abstract
In Brazil, serologic evidence of Orthopoxvirus (OPV) circulation showed positivity around 20% in cattle, humans, monkeys and rodents. Although OPV seropositivity has been described in buffalo herds in southeastern Brazil, no Vaccinia virus (VACV) (member of genus OPV) outbreaks in buffalo herds have been described in this country. This study aimed to investigate the detection of anti-OPV antibodies and to study the OPV genome in Brazilian buffalo herds. Our results demonstrated a high OPV seropositivity in buffalo herds on Marajó Island and molecular data confirmed the circulation of VACV. The geographical isolation conditionmight be a sine qua non condition to explain our results., (Copyright © 2016. Published by Elsevier Ltd.)
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- 2016
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22. Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil.
- Author
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Albernaz TT, Leite RC, Reis JK, de Sousa Rodrigues AP, da Cunha Kassar T, Resende CF, de Oliveira CH, Silva Rd, Salvarani FM, and Barbosa JD
- Subjects
- Animals, Brazil epidemiology, Cambodia, Cattle, Cattle Diseases epidemiology, Cloning, Molecular, DNA, Viral isolation & purification, Genome, Viral, Immunodeficiency Virus, Bovine genetics, Lentivirus Infections epidemiology, Leukocytes cytology, Pakistan epidemiology, Polymerase Chain Reaction veterinary, Risk Factors, Buffaloes virology, Cattle Diseases diagnosis, Immunodeficiency Virus, Bovine isolation & purification, Lentivirus Infections veterinary
- Abstract
Bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. Although the infection has been described in cattle in some countries, including in Brazil, there are only two reports of infection in buffaloes: one in Pakistan and one in Cambodia. The aim of the present study was to survey the occurrence of bovine immunodeficiency virus (BIV) in water buffaloes from the Amazon region, Pará state, Brazil. BIV proviral DNA was surveyed in 607 whole blood samples of water buffaloes from 10 farms located in the state of Pará using semi-nested polymerase chain reaction (PCR) (PCR-SN) to amplify the pol region of the viral genome. Of the 607 samples tested, 27 (4.4 %) were positive for BIV proviral DNA. The amplified fragments were confirmed by sequence analysis after cloning and nucleotide sequencing. The sequence obtained had 99 % similarity to the reference strain (R-29). The present study provides important epidemiological data because BIV was detected for the first time in water buffaloes in Brazil. Further, the results suggest the possibility of the virus being a risk factor for herd health because it may be a potential causal agent of chronic disease and, also may be associated to other infectious diseases.
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- 2015
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23. Bovine herpesvirus 6 in buffaloes (Bubalus bulalis) from the Amazon region, Brazil.
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de Oliveira CH, de Oliveira FG, Gasparini MR, Galinari GC, Lima GK, Fonseca AA Jr, Barbosa JD, Barbosa-Stancioli EF, Leite RC, and Dos Reis JK
- Subjects
- Animals, Brazil epidemiology, Buffaloes, DNA, Viral genetics, Herpesviridae Infections epidemiology, Herpesviridae Infections virology, Phylogeny, Polymerase Chain Reaction veterinary, Varicellovirus genetics, Herpesviridae Infections veterinary, Varicellovirus isolation & purification
- Abstract
This study presents the first description of Bovine herpesvirus 6 (BoHV-6) that was isolated from buffaloes of Amazon region in Brazil. Phylogenetic analysis showed that the BoHV-6 Brazilian strains clustered with the sequence of BoHV-6 from elsewhere available at the GenBank. It was observed in some buffaloes with lymphoproliferative disease in one herd, thus the animals were also tested for Bovine leukemia virus (BLV), which has been associated to lymphoma in bovines. All animals were negative to BLV. These results indicate that BoHV-6 is present in buffaloes in Brazil, but the importance and impact of this infection and its association with any illness is still undefined.
- Published
- 2015
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24. Reemergence of vaccinia virus during Zoonotic outbreak, Pará State, Brazil.
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de Assis FL, Vinhote WM, Barbosa JD, de Oliveira CH, de Oliveira CM, Campos KF, Silva NS, and Trindade Gde S
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- Animals, Brazil epidemiology, Cattle, Cattle Diseases epidemiology, Cattle Diseases virology, Genes, Viral, Geography, Medical, Humans, Phylogeny, Vaccinia pathology, Disease Outbreaks, Vaccinia epidemiology, Vaccinia veterinary, Vaccinia virus classification, Vaccinia virus genetics, Zoonoses epidemiology
- Abstract
In 2010, vaccinia virus caused an outbreak of bovine vaccinia that affected dairy cattle and rural workers in Pará State, Brazil. Genetic analyses identified the virus as distinct from BeAn58058 vaccinia virus (identified in 1960s) and from smallpox vaccine virus strains. These findings suggest spread of autochthonous group 1 vaccinia virus in this region.
- Published
- 2013
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25. Hepatic photosensitization in buffaloes intoxicated by Brachiaria decumbens in Minas Gerais state, Brazil.
- Author
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De Oliveira CH, Barbosa JD, Oliveira CM, Bastianetto E, Melo MM, Haraguchi M, Freitas LG, Silva MX, and Leite RC
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- Animals, Brazil epidemiology, Liver metabolism, Liver pathology, Rain, Saponins analysis, Seasons, Skin pathology, gamma-Glutamyltransferase blood, Brachiaria chemistry, Buffaloes, Disease Outbreaks veterinary, Photosensitivity Disorders chemically induced, Photosensitivity Disorders epidemiology, Photosensitivity Disorders veterinary, Saponins toxicity
- Abstract
The aim of this paper is to report the study of hepatogenous photosensitization in buffaloes during two outbreaks provoked by ingestion of Brachiaria decumbens in Minas Gerais state, Brazil. Ten young buffaloes in outbreak 1 and seven buffaloes in outbreak 2 were intoxicated by B. decumbens. Nine clinically healthy buffaloes raised under the same conditions as the sick animals served as the control group. All animals were subjected to clinical examination, and serum was collected to measure gamma-glutamyl transferase (GGT), aspartate aminotransferase (AST), direct bilirubin (DB), indirect bilirubin (IB) and total bilirubin (TB) as indicators of liver function and urea and creatinine as indicators of renal function. Histopathology of liver fragments from five different animals was carried out. During the outbreaks and every two months for one year, samples of grass from paddocks where the animals got sick were collected for quantitative evaluation of the saponin protodioscin, combined with observations of pasture characteristics and daily rainfall. Clinical signs included apathy, weight loss, restlessness, scar retraction of the ears and intense itching at the skin lesions, mainly on the rump, the tail head, neck and hindlimbs, similar to the signs observed in other ruminants. Only the GGT enzyme presented significantly different (P < 0.01) serum levels between intoxicated animals (n = 17) and healthy animals (n = 9), indicating liver damage in buffaloes bred in B. decumbens pastures. Microscopy of the liver showed foamy macrophages and lesions of liver disease associated with the presence of crystals in the bile ducts, which have also been found in sheep and cattle poisoned by grasses of the genus Brachiaria. During the outbreaks, protodioscin levels were higher than 3%, and shortly after, these levels were reduced to less than 0.80%, suggesting a hepatic injury etiology. The outbreaks took place at the beginning of the rainy season, and there was a positive correlation between saponin and the amount of rainfall, as well as between saponin and the amount of green leaves in the pasture. These findings indicate that the grass was more toxic in this period. This is the first report of photosensitization by B. decumbens in buffalo., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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26. Effects of perioperative hypothermia on healing of anastomosis of the colon in rats.
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de Oliveira JC, de Oliveira CH, de Oliveira HE, Colombeli GL, De Bona Heck N, Pereira A, and D'Acâmpora AJ
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- Anastomosis, Surgical, Animals, Colon metabolism, Cytokines genetics, Cytokines metabolism, Gene Expression Regulation, Hydroxyproline metabolism, Intercellular Signaling Peptides and Proteins genetics, Intercellular Signaling Peptides and Proteins metabolism, Male, Pressure, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Rats, Wistar, Colon pathology, Colon surgery, Hypothermia, Induced, Perioperative Care, Wound Healing
- Abstract
Objective: We evaluated the hypothesis that induced perioperative hypothermia in rats causes adverse effects on the healing of colonic anastomosis., Materials and Methods: Forty-eight Wistar rats were divided into eight groups of six animals that underwent laparotomy, sigmoid section, and anastomosis. Four groups were operated under normothermic conditions (36 ± 1 °C) and four under hypothermic conditions (32 ± 1 °C). The reoperations were performed on days 3, 7, and 14 post-surgery, and two groups where SHAM reoperated on day 3. We evaluated anastomotic bursting pressure and tissue hydroxyproline content; performed a histological analysis of inflammatory parameters and healing (inflammatory cell infiltrate, edema, fibrin, collagen deposition and apoptotic cells) with categorization scores = 0, 1, 2, 3; and examined the relative quantification gene expression (cDNA) of inflammatory cytokines [interleukin 1 (IL-1), interleukin 6 (IL-6), and interleukin 10 (IL-10)] and growth factors [vascular endothelial growth factor and insulin-like growth factor 1 (IGF-1)] by reverse transcription polymerase chain reaction., Results: Both of the hypothermic groups showed lower anastomotic burst pressure on days 7 and 14 post-surgery, reduced hydroxyproline content on day 14, reduction of inflammatory infiltrates and edema at day 3, and less collagen deposition on day 14. In animals that were hypothermic, the cytokine gene expression showed reduced IL-1 on day 3, reduced IL-6 on days 7 and 14, and reduced IL-10 on days 7 and 14 and a reduction in the growth factor IGF-1 on day 7., Conclusion: Perioperative hypothermia had detrimental effects on the healing of colonic anastomosis in rats.
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- 2013
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27. Multifocal cutaneous ORF virus infection in goats in the Amazon region, Brazil.
- Author
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de Oliveira CH, Assis FL, Neto JD, Oliveira CM, Lopes CT, Bomjardim Hdos A, Vinhote WM, Silva AG, Abrahão JS, and Kroon EG
- Subjects
- Amino Acid Sequence, Animals, Brazil epidemiology, Ecthyma, Contagious epidemiology, Ecthyma, Contagious pathology, Ecthyma, Contagious virology, Genes, Viral, Goat Diseases pathology, Goat Diseases virology, Lip Diseases epidemiology, Lip Diseases pathology, Lip Diseases veterinary, Lip Diseases virology, Molecular Sequence Data, Orf virus classification, Orf virus genetics, Phylogeny, Sequence Alignment, Skin Diseases, Infectious epidemiology, Skin Diseases, Infectious virology, Goat Diseases epidemiology, Goats virology, Orf virus isolation & purification, Orf virus pathogenicity, Skin Diseases, Infectious veterinary
- Abstract
Orf virus is the etiological agent of contagious ecthyma, a severe exanthematic disease that affects small ruminants. Orf virus is zoonosis that is associated with occupational contact with infected animals in human disease. Clinically, contagious ecthyma is characterized by the appearance of vesicles, pustules, ulcers, and papillomatous proliferative lesions on the skin of the lips and nostrils. Here we describe a case of lethal cutaneous multifocal Orf virus infection in goats in the Amazon region of Brazil. Exanthematic lesions were collected and epidemiological and clinical data were obtained. Orf virus was detected using PCR amplification of the whole B2L, VIR, and VEGF open reading frame. Phylogenetic analysis revealed that this virus clustered together with the Orf virus samples isolated during classical contagious ecthyma. The present work is the first to report a severe proliferative Orf virus case in South America.
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- 2012
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28. Complete assignment of NMR data of 22 phenyl-1H-pyrazoles' derivatives.
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de Oliveira AL, Alves de Oliveira CH, Mairink LM, Pazini F, Menegatti R, and Lião LM
- Subjects
- Carbon Isotopes, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Phenols chemical synthesis, Pyrazoles chemical synthesis, Reference Standards, Phenols chemistry, Protons, Pyrazoles chemistry
- Abstract
Complete assignment of (1)H and (13)C NMR chemical shifts and J((1)H/(1)H and (1)H/(19)F) coupling constants for 22 1-phenyl-1H-pyrazoles' derivates were performed using the concerted application of (1)H 1D and (1)H, (13)C 2D gs-HSQC and gs-HMBC experiments. All 1-phenyl-1H-pyrazoles' derivatives were synthesized as described by Finar and co-workers. The formylated 1-phenyl-1H-pyrazoles' derivatives were performed under Duff's conditions., (Copyright © 2011 John Wiley & Sons, Ltd.)
- Published
- 2011
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29. The effect of temperature on self-etching adhesive penetration.
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Sundfeld RH, da Silva AM, Croll TP, de Oliveira CH, Briso AL, de Alexandre RS, and Sundefeld ML
- Subjects
- Acid Etching, Dental methods, Adolescent, Adult, Analysis of Variance, Humans, Surface Properties, Temperature, Dental Bonding methods, Dental Enamel Permeability, Resin Cements
- Abstract
The purpose of this study was to investigate the penetration of an aggressive self-etching adhesive system at refrigerated and room temperatures into ground and unground enamel surfaces. Thirty extracted human teeth were used to measure adhesive penetration into enamel by light microscopy analysis (x400). The unground enamel surfaces were cleaned with pumice and water using a rotary dental brush. For each specimen, part of the unground enamel was manually ground and part was kept intact. A self-etch adhesive was evaluated for its ability to penetrate ground and unground enamel surfaces at room temperature (25 degrees C), at 30 minutes after removal from the refrigerator, and immediately after removal from the refrigerator (6 degrees C). Data were analyzed using variance and the Tukey test, which revealed significant differences in length of penetration of this material when applied on ground and unground enamel surfaces and between the different temperatures used (P > .05). The self-etching system used in this study had significantly lower penetration into unground enamel and at 6 degrees C (P < .05). No statistical difference was found between the interactions of these factors. It was concluded that the self-etching system produced the best penetration into ground enamel surface at room temperature (25 degrees C) and at 30 minutes after removing the specimens from the refrigerator.
- Published
- 2006
30. Distribution of HOMA-IR in Brazilian subjects with different body mass indexes.
- Author
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Ghiringhello MT, Vieira JG, Tachibana TT, Ferrer C, Maciel RM, Amioka PH, Hauache OM, de Oliveira CH, Khawali C, and Reis AF
- Subjects
- Blood Glucose analysis, Brazil, Fasting blood, Humans, Insulin blood, Reference Values, Retrospective Studies, Body Mass Index, Insulin Resistance
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- 2006
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31. Mites in dust samples from mattress surfaces from single beds or cribs in the south Brazilian city of Londrina.
- Author
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da Silva DR, Binotti RS, da Silva CM, de Oliveira CH, Condino-Neto A, and de Capitani EM
- Subjects
- Adult, Animals, Brazil, Child, Humans, Beds microbiology, Dust analysis, Infant Equipment microbiology, Mites microbiology
- Abstract
The aim of this study was to investigate the mite fauna in mattresses dust samples from cribs or beds in the south Brazilian city of Londrina, State of Parana. A total of 133 dust samples from upper and lower mattress surfaces, and bed frames were aspirated once from 38 dwellings (18 cribs and 21 beds), and one day nursery (six cribs). A total of 758 mite bodies were counted in slides: 233 (30.7%) from cribs and 525 (69.3%) from beds (p<0.001). House dust mites--mainly Dermatophagoides pteronyssinus, represented 72% and 84% of total mite count in crib and bed dust samples, respectively. The mean HDM body concentration in crib or bed slides were, respectively, 289.9+/-136.7 and 875.0+/-183.6 mites/g. Statistical analysis showed a significantly higher mite bodies count on lower mattress surface compared with upper surface in bed samples only (p=0.025). Data herein show that cribs like mattress have sufficient mite bodies to cause sensitization to humans. The use of mattress covers for cribs and beds should be encouraged in order to avoid allergens exposure., (Copyright (c) 2005 Blackwell Munksgaard)
- Published
- 2005
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32. Cyclosporine bioequivalence study: quantification using fluorescence polarization immunoassay (FPIA) and radioimmunoassay (RIA).
- Author
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Mendes GD, de Oliveira CH, Sucupira M, Donato JL, Moreno RA, and De Nucci G
- Subjects
- Administration, Oral, Adolescent, Adult, Area Under Curve, Capsules, Chemistry, Pharmaceutical, Cross-Over Studies, Cyclosporine administration & dosage, Cyclosporine blood, Humans, Immunosuppressive Agents administration & dosage, Immunosuppressive Agents blood, Male, Middle Aged, Therapeutic Equivalency, Cyclosporine pharmacokinetics, Immunosuppressive Agents pharmacokinetics
- Abstract
Objective: The aim of study was to compare the bioavailability of 2 cyclosporine capsule formulations (100 mg; Sigmasporin Microoral from Novaquímica Divisão Nature's Plus Farmacêutica Ltd., Brazil, as test formulation and Sandimmune Neoral from Novartis Biociências S.A., Brazil, as reference formulation) in 24 healthy male volunteers., Methods: The study was open, randomized, with a 2-period crossover, a 1-week washout interval between doses. Blood samples were obtained over a 12-hour interval after each oral administration of cyclosporine (2 capsules of 100 mg of each formulation). Cyclosporine blood concentrations were quantified using a fluorescence polarization immunoassay (FPIA) method provided by Abbott Axsym System and Cyclo-Trac SP. Whole-blood radioimmuoassay (RIA) kit was provided by DiaSorin. These assays provided concentration-time curves for cyclosporine in blood concentration from which the following pharmacokinetic parameters were obtained: AUC(last), AUC(inf), Cmax., Results: Geometric mean and 90% confidence intervals (CI) of Microoral/Neoral as percent ratios were 94.5% (90.8-98.4%) for AUC(last), 93.8% (89.7-98.1%) for AUC(inf), and 98.1% (94.5-101.8%) for Cmax when cyclosporine was determined using FPIA and 96.1% (91.9 to 100.6%) for AUC(last), 95.2% (90.2-100.5%) for AUC(inf), and 99.4% (96.4-102.4%) for Cmax using RIA., Conclusion: Since the 90% CI for Cmax, AUC(last) and AUC(inf) ratio were within the 80-125% interval proposed by US-FDA, it is concluded that Sigmasporin Microoral 100 mg capsule formulation is bioequivalent to Sandimmune Neoral 100 mg capsule formulation with regard to both rate and the extent of absorption.
- Published
- 2004
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33. Comparison of house dust mites found on different mattress surfaces.
- Author
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de Oliveira CH, Binotti RS, Muniz JR, dos Santos JC, do Prado AP, and de Pinho AJ Jr
- Subjects
- Air Pollution, Indoor analysis, Animals, Bedding and Linens, Brazil, Floors and Floorcoverings, Interior Design and Furnishings, Allergens analysis, Beds, Pyroglyphidae classification
- Abstract
Background: House dust mites have been considered the most important source of allergens for humans. These allergens have been encountered at different indoor sites, mainly on mattresses and pillows., Objective: To evaluate the number and different specimens of mites on Brazilian bunk-bed mattresses., Methods: Dust samples were collected once using a standardized method on the upper mattress surface (US) and lower mattress surface (LS) (bed frame also included in the latter) of 58 mattresses., Results: The total number of mite bodies on the LS was 3.5-fold higher than on the US, with a mean concentration of 932 mites per gram of fine dust (mites/g) on the US (range, 0-3,375 mites/g) and 3,254 mites/g on the LS (range, 125-14,500 mites/g) (P < .001). Additionally, the number of house dust mite bodies on the LS was 2.4 higher than on the US (P < .001); the mean concentration was 750 mites/g on the US (range, 0-2,875 mites/g) and 1,816 mites/g on the LS (range, 0-10,875 mites/g). Approximately 91% (n = 52) of the US and all LS dust samples had more than the limit of 100 mites/g. The most frequent family was Pyroglyphidae in both mattress surfaces, with Dermatophagoides pteronyssinus the most important species found. Storage mites, including Glycyphagidae (P < .001), Acaridae (P < .001), and other families (P < .001), also showed significant differences in the number of mites between both sample counts., Conclusions: This study shows a significant difference in US and LS mite counts, with higher counts on the LS. Mite allergen avoidance should include the LS and bed frame as potential targets.
- Published
- 2003
- Full Text
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34. Terbinafine quantification in human plasma by high-performance liquid chromatography coupled to electrospray tandem mass spectrometry: application to a bioequivalence study.
- Author
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de Oliveira CH, Barrientos-Astigarraga RE, de Moraes MO, Bezerra FA, de Moraes ME, and de Nucci G
- Subjects
- Chromatography, High Pressure Liquid, Humans, Spectrometry, Mass, Electrospray Ionization, Terbinafine, Therapeutic Equivalency, Antifungal Agents blood, Naphthalenes blood
- Abstract
A method based on liquid chromatography with positive ion electrospray ionization and tandem mass spectrometry is described for the determination of terbinafine in human plasma using naftifine as internal standard. The method has a chromatographic run time of 5 minutes and was linear in the range 1.0 to 2000 ng/mL. The limit of quantification was 1.0 ng/mL; the intraday precision was 3.6%, 3.8%, 3.5%, and 4.1%; and the intraday accuracy was -2.7%, 7.7%, 4.8%, and -2.7% for 5.0, 80.0, 250.0, and 1500.0 ng/mL, respectively. The interday precision was 4.9%, 1.7%, 2.4%, and 4.6% and the interday accuracy was 0.3%, 5.8%, 6.5%, and -1.4% for the same concentrations. This method was used in a bioequivalence study of two tablet formulations of terbinafine. Twenty-four healthy volunteers (both sexes) received a single oral dose of terbinafine (250 mg) in an open, randomized, two-period crossover study. The 90% CI of geometric mean ratios between Terbinafina (Medley S/A Indústria Farmacêutica, Campinas, Brazil) and Lamisil (Novartis Biociências S/A, São Paulo, Brazil) were 90.5% to 110.0% for C max, 92.2% to 108.1% for AUC last, and 91.3% to 107.5% for AUC 0-inf. Because the 90% CI for the above-mentioned parameters were included in the 80% to 125% interval proposed by the US FDA, the two formulations were considered bioequivalent in terms of rate and extent of absorption.
- Published
- 2001
- Full Text
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35. Placental and fetal candidiasis. Presentation of a case of an abortus.
- Author
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Bittencourt AL, dos Santos WL, and de Oliveira CH
- Subjects
- Abortion, Septic etiology, Adult, Candidiasis, Vulvovaginal complications, Female, Fetal Diseases etiology, Humans, Intrauterine Devices adverse effects, Male, Placenta Diseases etiology, Pregnancy, Candidiasis pathology, Fetal Diseases pathology, Placenta Diseases pathology, Pregnancy Complications, Infectious pathology
- Abstract
The authors present a case of intrauterine fetal infection by candida, in an abortion of four months, associated with an I.U.D. In the placenta and adnexa we observed an acute inflammation consisting of extraplacental membranitis, omphalitis, chorio-amnionitis and choriovasculitis with a marked villitis and intervillitis. In the fetus, involvement of the skin, lungs and pharynx was observed. This case represents, probably the 15th reported instance of congenital fetal candidiasis, and the first case of a candida hematogenic placental infection acquired from the fetal blood. The fetus undoubtedly acquired its infection by an ascending route, through the contamined amniotic fluid.
- Published
- 1984
- Full Text
- View/download PDF
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