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Heat stress is an increasingly concerning topic under global warming. Heat stress can induce organisms to produce excess reactive oxygen species, which will lead to cell damage and destroy the antioxidant defense of aquatic animals. Chinese mitten crab, Eriocheir sinensis, is sensitive to the change in water temperature, and parent crabs are more vulnerable during the breeding stage. In the present study, the multi-omics responses of parent E. sinensis gills to heat stress (24 h) were determined via transcriptome and proteome. The integrative analysis revealed that heat shock protein 70 (HSP70) and glutathione s-transferase (GST) were significantly up-regulated at gene and protein levels after heat stress, indicating that HSP70 and the antioxidant system participated in the regulatory mechanism of heat stress to resist oxidative damage. Moreover, the "Relaxin signaling pathway" was also activated at gene and protein levels under 30 °C stress, which implied that relaxin may be essential and responsible for reducing the oxidative damage of gills caused by extreme heat stress. These findings provided an understanding of the regulation mechanism in E. sinensis under heat stress at gene and protein levels. The mining of key functional genes, proteins, and pathways can also provide a basis for the cultivation of new varieties resistant to oxidative stress. [ABSTRACT FROM AUTHOR]

Chromium (Cr) is a toxic heavy element that interferes with plant metabolite biosynthesis and modifies the plant rhizosphere microenvironment, affecting plant growth. However, the interactions and response mechanisms between plants and rhizosphere bacteria under Cr stress still need to be fully understood. In this study, we used Iris tectorum as a research target and combined physiology, metabolomics, and microbiology to reveal the stress response mechanism of I. tectorum under heavy metal chromium stress. The results showed that Cr stress-induced oxidative stress inhibited plant growth and development and increased malondialdehyde and oxygen free radicals content. Also, it increased ascorbate peroxidase, peroxidase activity, and superoxide dismutase activity, as well as glutathione and soluble sugar content. Microbiome analysis showed that Cr stress changed the rhizosphere bacterial community diversity index by 33.56%. Proteobacteria, Actinobacteriota, and Chloroflexi together accounting for 71.21% of the total sequences. Meanwhile, the abundance of rhizosphere dominant and plant-promoting bacteria increased significantly with increasing time of Cr stress. The improvement of the soil microenvironment and the recruitment of bacteria by I. tectorum root secretions were significantly enhanced. By metabolomic analysis, five vital metabolic pathways were identified, involving 89 differentially expressed metabolites, divided into 15 major categories. In summary, a multi-omics approach was used in this study to reveal the interaction and stress response mechanisms between I. tectorum and rhizosphere bacterial communities under Cr stress, which provided theoretical basis for plant-microbial bioremediation of Cr-contaminated soils in constructed wetlands. This may provide more valuable information for wetland remediation of heavy metal pollution.

Recent pharmacological studies have shown that dragon's blood has an anti-cerebral ischemia effect. Loureirin C (LC), a kind of dihydrochalcone compound in dragon's blood, is believed to be play an important role in the treatment of ischemia stroke, but fewer studies for LC have been done. In this paper, we report the first experimental and theoretical studies on the antioxidation mechanism of LC by radical scavenging. The experimental studies show that LC has almost no effect on cell viability under 15 μM for the SH-SY5Y cells without any treatments. For the SH-SY5Y cells with oxygen and glucose deprivation-reperfusion (OGD/R) treatment, LC increased the viability of SH-SY5Y cells. The results of 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) and MitoSox Red experiments indicate that LC is very efficient in inhibiting the generation of the intracellular/mitochondrial reactive oxygen species (ROS) or removing these two kinds of generated ROS. The density functional theory (DFT) calculations allowed us to elucidate the antioxidation mechanisms of LC. Fukui function analysis reveals the radical scavenging of LC by hydrogen abstraction mechanism, the complex formation by e-transfer, and radical adduct formation (RAF) mechanism. Among the H-abstraction, the complex formation by e-transfer, and radical adduct formation (RAF) reactions on LC, the H-abstraction at O-H35 position by OH• is favorable with the smallest energy difference between the product and two reactants of the attack of OH• to LC of −0.0748 Ha. The bond dissociation enthalpies (BDE), proton affinities (PA), ionization potential (IP), proton dissociation enthalpy (PDE), and electron transfer enthalpy (ETE) were calculated to determine thermodynamically preferred reaction pathway for hydrogen abstraction mechanism. In water, IP and the lowest PDE value at O3-H35 position are lower than the lowest BDE value at O3-H35 position; 41.8986 and 34.221 kcal/mol, respectively, indicating that SEPT mechanism is a preferred one in water in comparison with the HAT mechanism. The PA value of O3-H35 of LC in water is −17.8594 kcal/mol, thus the first step of SPLET would occur spontaneously. The minimum value of ETE is higher than the minimum value of PDE at O3-H35 position and IP value, 14.7332 and 22.4108 kcal/mol, respectively, which suggests that the SEPT mechanism is a preferred one in water in comparison with the SPLET mechanism. Thus, we can draw a conclusion that the SEPT mechanism of is the most favorite hydrogen abstraction mechanism in water, and O-H35 hydroxyl group has the greatest ability to donate H-atoms. [ABSTRACT FROM AUTHOR]

Heat stress is an increasingly concerning topic under global warming. Heat stress can induce organisms to produce excess reactive oxygen species, which will lead to cell damage and destroy the antioxidant defense of aquatic animals. Chinese mitten crab, Eriocheir sinensis, is sensitive to the change in water temperature, and parent crabs are more vulnerable during the breeding stage. In the present study, the multi-omics responses of parent E. sinensis gills to heat stress (24 h) were determined via transcriptome and proteome. The integrative analysis revealed that heat shock protein 70 (HSP70) and glutathione s-transferase (GST) were significantly up-regulated at gene and protein levels after heat stress, indicating that HSP70 and the antioxidant system participated in the regulatory mechanism of heat stress to resist oxidative damage. Moreover, the “Relaxin signaling pathway” was also activated at gene and protein levels under 30 °C stress, which implied that relaxin may be essential and responsible for reducing the oxidative damage of gills caused by extreme heat stress. These findings provided an understanding of the regulation mechanism in E. sinensis under heat stress at gene and protein levels. The mining of key functional genes, proteins, and pathways can also provide a basis for the cultivation of new varieties resistant to oxidative stress.

Generally, MoS2 is easily oxidized when exposed to oxygen, and the antioxidation mechanism of MoS2 is still a challenge. Thus, more efforts were made to greatly improve its antioxidation performance. It was reported that the Ti atom doped with MoS2 was treated as the effective method to enhance its antioxidation performance; however, the detailed antioxidation mechanism was not well understood. Superior to experimental methods, the first-principles method could provide deep insight into the atomic information and serve as a useful tool to gain an understanding of the antioxidation mechanisms of the doped MoS2; thus, the antioxidation behavior of the Ti-doped MoS2 was investigated in detail using first-principles calculations. However, an opposing conclusion was obtained from the calculated results compared to the previous experimental results; that is, the incorporation of the Ti atom was not helpful for improving the antioxidation performance of MoS2. The strange phenomenon was well probed and discussed in detail, and understanding the oxidation mechanism of the Ti-doped MoS2 would be helpful for expanding its applications in the ambient atmosphere. [ABSTRACT FROM AUTHOR]

Recent pharmacological studies have shown that dragon’s blood has an anti-cerebral ischemia effect. Loureirin C (LC), a kind of dihydrochalcone compound in dragon’s blood, is believed to be play an important role in the treatment of ischemia stroke, but fewer studies for LC have been done. In this paper, we report the first experimental and theoretical studies on the antioxidation mechanism of LC by radical scavenging. The experimental studies show that LC has almost no effect on cell viability under 15 μM for the SH-SY5Y cells without any treatments. For the SH-SY5Y cells with oxygen and glucose deprivation-reperfusion (OGD/R) treatment, LC increased the viability of SH-SY5Y cells. The results of 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) and MitoSox Red experiments indicate that LC is very efficient in inhibiting the generation of the intracellular/mitochondrial reactive oxygen species (ROS) or removing these two kinds of generated ROS. The density functional theory (DFT) calculations allowed us to elucidate the antioxidation mechanisms of LC. Fukui function analysis reveals the radical scavenging of LC by hydrogen abstraction mechanism, the complex formation by e-transfer, and radical adduct formation (RAF) mechanism. Among the H-abstraction, the complex formation by e-transfer, and radical adduct formation (RAF) reactions on LC, the H-abstraction at O-H35 position by OH• is favorable with the smallest energy difference between the product and two reactants of the attack of OH• to LC of −0.0748 Ha. The bond dissociation enthalpies (BDE), proton affinities (PA), ionization potential (IP), proton dissociation enthalpy (PDE), and electron transfer enthalpy (ETE) were calculated to determine thermodynamically preferred reaction pathway for hydrogen abstraction mechanism. In water, IP and the lowest PDE value at O3-H35 position are lower than the lowest BDE value at O3-H35 position; 41.8986 and 34.221 kcal/mol, respectively, indicating that SEPT mechanism is a preferred one in water in comparison with the HAT mechanism. The PA value of O3-H35 of LC in water is −17.8594 kcal/mol, thus the first step of SPLET would occur spontaneously. The minimum value of ETE is higher than the minimum value of PDE at O3-H35 position and IP value, 14.7332 and 22.4108 kcal/mol, respectively, which suggests that the SEPT mechanism is a preferred one in water in comparison with the SPLET mechanism. Thus, we can draw a conclusion that the SEPT mechanism of is the most favorite hydrogen abstraction mechanism in water, and O-H35 hydroxyl group has the greatest ability to donate H-atoms.

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In this study, poly(p‐phenylenediamine) (PPDA) was synthesized by an enzymatic polymerization method using p‐phenylenediamine as the monomer in the water phase. The PPDA structure was characterized by infrared (IR), gel permeation chromatography, and elemental analysis. The antioxidation behavior of the as‐synthesized PPDA in biodegradable castor oil was investigated using a rotary oxygen bomb test and pressurized differential scanning calorimetry. A theoretical calculation was conducted to investigate its antioxidation mechanism. PPDA was found to exhibit excellent oxidation resistance in castor oil at 150 °C, which effectively prolonged the oxidation induction time of the oil. The results obtained herein suggest that as with phenolic antioxidants, PPDA with a hindered structure effectively improves the antioxidation performance of the oil by enhancing the stability of nitrogen free radicals. [ABSTRACT FROM AUTHOR]

Cd and Pb are a toxic environmental pollutant, and their elevated concentrations in the waters and soils could exert detriment effects on human health by food chain. In order to evaluate the capacity to heavy metal accumulation and the physiochemical responses of two Salix genotypes, a 35-day hydroponic seedling experiment was implemented with Salix matsudana Koidz. 'Shidi1' (A42) and Salix psammophila C. 'Huangpi1' (A94) under different concentrations of Cd (15 and 30 μM) or Pb (250 and 300 μM). The results showed that the biomass of A94 severely reduced more than that of A42. The accumulation ability of Cd in different plant organs followed the sequence of leaves > roots > stems. Pb primarily accumulated in the roots for both Salix genotypes (54.27 mg g−1 for A42 and 54.52 mg g−1 for A94). Translocation factors based on accumulation (TF′) for Cd were more than 8.0, while TF′s for Pb were less than 1.0 in both A42 and A94, implying they could be applied in the phytoremediation of Cd-contaminated sites due to their stronger ability to Cd phytoextraction. The stress of Cd or Pb significantly increased malondialdehyde (MDA) contents and increased photosynthetic rates in leaves of two Salix genotypes. Transpiration rates of willow were positively correlated with its Cd translocation. Both catalase (CAT) and peroxidase (POD) activities were suppressed, while the superoxide dismutase (SOD) was boosted with increasing Cd and Pb levels in the leaves and roots of the two willow genotypes, suggesting SOD plays an important role in the removal of ROS. The inconsistency of the changes in enzyme activity suggests that the integrated antioxidative mechanisms regulate the tolerance to Cd and Pb stress. [ABSTRACT FROM AUTHOR]

Chromium (Cr) is a toxic heavy element that interferes with plant metabolite biosynthesis and modifies the plant rhizosphere microenvironment, affecting plant growth. However, the interactions and response mechanisms between plants and rhizosphere bacteria under Cr stress still need to be fully understood. In this study, we used Iris tectorum as a research target and combined physiology, metabolomics, and microbiology to reveal the stress response mechanism of I. tectorum under heavy metal chromium stress. The results showed that Cr stress-induced oxidative stress inhibited plant growth and development and increased malondialdehyde and oxygen free radicals content. Also, it increased ascorbate peroxidase, peroxidase activity, and superoxide dismutase activity, as well as glutathione and soluble sugar content. Microbiome analysis showed that Cr stress changed the rhizosphere bacterial community diversity index by 33.56%. Proteobacteria , Actinobacteriota , and Chloroflexi together accounting for 71.21% of the total sequences. Meanwhile, the abundance of rhizosphere dominant and plant-promoting bacteria increased significantly with increasing time of Cr stress. The improvement of the soil microenvironment and the recruitment of bacteria by I. tectorum root secretions were significantly enhanced. By metabolomic analysis, five vital metabolic pathways were identified, involving 89 differentially expressed metabolites, divided into 15 major categories. In summary, a multi-omics approach was used in this study to reveal the interaction and stress response mechanisms between I. tectorum and rhizosphere bacterial communities under Cr stress, which provided theoretical basis for plant-microbial bioremediation of Cr-contaminated soils in constructed wetlands. This may provide more valuable information for wetland remediation of heavy metal pollution. [Display omitted] • Cr stress increased the content of antioxidant enzymes and inhibited the synthesis of photosynthetic pigments. • Cr stress changed the composition of the Iris tectorum rhizosphere bacterial community structure. • Cr stress increased the content of fatty acyls, sphingolipids, terpenoids and flavonoids. • Cr stress affected Sphingolipid metabolism, Glycine, serine and threonine metabolism, Glycerophospholipid metabolism and Arachidonic acid metabolism. [ABSTRACT FROM AUTHOR]

Low temperature is a major adverse environmental factor that impairs petunia growth and development. To better understand the molecular mechanisms of cold stress adaptation of petunia plants, a quantitative proteomic analysis using iTRAQ technology was performed to detect the effects of cold stress on protein expression profiles in petunia seedlings which had been subjected to 2°C for 5d. Of the 2,430 proteins whose levels were quantitated, a total of 117 proteins were discovered to be differentially expressed under low temperature stress in comparison to unstressed controls. As an initial study, 44 proteins including well known and novel cold-responsive proteins were successfully annotated. By integrating the results of two independent Gene Ontology (GO) enrichment analyses, seven common GO terms were found of which oxidation-reduction process was the most notable for the cold-responsive proteins. By using the subcellular localization tool Plant-mPLoc predictor, as much as 40.2% of the cold-responsive protein group was found to be located within chloroplasts, suggesting that the chloroplast proteome is particularly affected by cold stress. Gene expression analyses of 11 cold-responsive proteins by real time PCR demonstrated that the mRNA levels were not strongly correlated with the respective protein levels. Further activity assay of anti-oxidative enzymes showed different alterations in cold treated petunia seedlings. Our investigation has highlighted the role of antioxidation mechanisms and also epigenetic factors in the regulation of cold stress responses. Our work has provided novel insights into the plant response to cold stress and should facilitate further studies regarding the molecular mechanisms which determine how plant cells cope with environmental perturbation.

A novel kind of Dy(III) complex with 2-mercaptobenzimidazole was prepared. The antioxidative properties and mechanisms of the Dy(III) complex in the NR vulcanizates were investigated by attenuated total-reflectance Fourier transform infrared (FTIR-ATR) spectroscopy, crosslinking density determination, mechanical properties testing and thermogravimetric analysis. The thermo-oxidative stability of the NR vulcanizates was found to be improved markedly by the addition of Dy(III) complex. It can be attributed to the thioether bonds, which can decompose the hydroperoxide, and the Dy(III) ions, which can capture and inactivate the oxy radicals. The apparent thermo-oxidation activation energy of the NR vulcanizates was calculated by the Kissinger and Flynn-Wall-Ozawa methods, respectively. The results show that the NR vulcanizates with the Dy(III) complex have higher thermo-oxidation activation energy than that of the samples with antioxidant MB and antioxidant 4010NA. [ABSTRACT FROM AUTHOR]

novel OxyR (DR0615) with one conserved cysteine that senses hydrogen peroxide in Deinococcus radiodurans had been identified in our previous work. Comparative genomics revealed that D. radiodurans possesses another OxyR homolog, OxyR (DRA0336). In this study, we constructed the deletion mutant of oxyR and the double mutant of both the OxyR homologs to investigate the role of OxyR in response to oxidative stress in D. Radiodurans. Deletion of oxyR resulted in an obviously increased sensitivity to hydrogen peroxide, and the double mutant for oxyR and oxyR was significantly more sensitive than any of the two single mutants. The total catalase activity of the double mutant was lower than that of any of the single mutants, and reactive oxygen species (ROS) accumulated to a greater extent. DNA microarray analysis further suggested that oxyR was involved in antioxidation mechanisms. Site-direct mutagenesis and complementation analysis revealed that C in OxyR was essential. This is the first report of the presence of two OxyR in one organism. These results suggest that D. radiodurans OxyR and OxyR function together to protect the cell against oxidative stress. [ABSTRACT FROM AUTHOR]

• The antioxidation mechanism of RuAl film is studied by first-principles calculation. • The influence of alloying elements on the oxidation resistance of RuAl (1 1 1) surface is studied. • Cr element improves the oxidation resistance of RuAl(1 1 1) surface. • Oxygen prefers to occupy the Ru-bridge site in comparison to other sites. • The calculated bond strength of Cr-O bond is stronger than that of Al-O bond. We apply the first-principles calculations to investigate the influence of Cr and Y on the oxidation resistance of RuAl(1 1 0) surface. Four possible oxygen adsorption sites: Al-top, Ru-top, Ru-bridge and Al-bridge are considered. It is found that the capacity of oxidation resistance of Ru is stronger than that of Al. Oxygen atom is adsorbed on the Ru-bridge site because of the strong charge interaction between Ru and the O. Importantly, the calculated adsorption energy of Cr-doped RuAl(1 1 0) surface is −0.350 eV, which is smaller than that of Ru-bridge site. The calculated result indicates that Cr-dopant can improve the oxidation resistance of RuAl(1 1 0) surface. The reason is that Cr-dopant improves the localized hybridization between Cr atom and the O atom, and weakens the charge interaction of Ru-O atoms and Al-O atoms. Therefore, we predict that Cr is a beneficial element to improve the oxidation resistance of RuAl thermal barrier coating. [ABSTRACT FROM AUTHOR]

Low temperature is a major adverse environmental factor that impairs petunia growth and development. To better understand the molecular mechanisms of cold stress adaptation of petunia plants, a quantitative proteomic analysis using iTRAQ technology was performed to detect the effects of cold stress on protein expression profiles in petunia seedlings which had been subjected to 2°C for 5 days. Of the 2430 proteins whose levels were quantitated, a total of 117 proteins were discovered to be differentially expressed under low temperature stress in comparison to unstressed controls. As an initial study, 44 proteins including well known and novel cold-responsive proteins were successfully annotated. By integrating the results of two independent Gene Ontology (GO) enrichment analyses, seven common GO terms were found of which "oxidation-reduction process" was the most notable for the cold-responsive proteins. By using the subcellular localization tool Plant-mPLoc predictor, as much as 40.2% of the cold-responsive protein group was found to be located within chloroplasts, suggesting that the chloroplast proteome is particularly affected by cold stress. Gene expression analyses of 11 cold-responsive proteins by real time PCR demonstrated that the mRNA levels were not strongly correlated with the respective protein levels. Further activity assay of anti-oxidative enzymes showed different alterations in cold treated petunia seedlings. Our investigation has highlighted the role of antioxidation mechanisms and also epigenetic factors in the regulation of cold stress responses. Our work has provided novel insights into the plant response to cold stress and should facilitate further studies regarding the molecular mechanisms which determine how plant cells cope with environmental perturbation. The data have been deposited to the ProteomeXchange with identifier PXD002189.