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Gut microbiota dysbiosis induced by acute pancreatitis (AP) exacerbates pancreatic injury and systemic inflammatory responses. The alleviation of gut microbiota dysbiosis through faecal microbiota transplantation (FMT) is considered a potential strategy to reduce tissue damage and inflammation in many clinical disorders. Here, we aim to investigate the effect of gut microbiota and microbiota-derived metabolites on AP and further clarify the mechanisms associated with pancreatic damage and inflammation.AP rat and mouse models were established by administration of caerulein or sodium taurocholate in vivo. Pancreatic acinar cells were exposed to caerulein and lipopolysaccharide in vitro to simulate AP.Normobiotic FMT alleviated AP-induced gut microbiota dysbiosis and ameliorated the severity of AP, including mitochondrial dysfunction, oxidative damage and inflammation. Normobiotic FMT induced higher levels of NADGut microbiota-derived NMN alleviates the severity of AP by activating the SIRT3-PRDX5 pathway. Normobiotic FMT could be served as a potential strategy for AP treatment.

Many State Grid projects of China are facing a series of natural disaster threats, such as landslide, collapse, mud-rock slide, etc. This article takes the Huangshi Daye Steel Substation as research object. Based on the orthogonal test which confirmed factors influencing the stability, it adopts the Numerical Simulation Method and the Roof Thickness-to-span Ratio Method to evaluate the stability of the Huangshi Daye Steel Substations karst foundation. By doing this, karst foundation stability maps of the substation are respectively obtained. Analyzing the stability results of the two methods, it has been found that the stability evaluation results measured by the two methods are the same. And orthogonal test has advantages such as comprehensiveness, speediness, reliability and accuracy. Meanwhile, according to the stability evaluation results, specific site operation suggestions are proposed.