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This study aimed to examine how soccer referees make decisions about issuing yellow cards for fouls. The research involved 60 male participants, divided into expert (n=30) and novice (n=30) groups based on their experience and qualifications as referees. They took part in a 2×2×2 mixed-design experiment. The study looked at Decision-Making Style (DMS: Analytical Decision-Making [ADM] vs. Intuitive Decision-Making [IDM]), Video Type (yellow card foul vs. non-yellow card foul), and Referee Level (expert vs. novice) as independent variables. The dependent variables were accuracy rate (ACC), discrimination index (D), self-confidence index (C), and overconfidence index (OC). The findings showed that Analytical Decision-Making (ADM) led to higher accuracy compared to Intuitive Decision-Making (IDM). Expert referees demonstrated better accuracy than novice referees. There was also an interaction between Decision-Making Style and Referee Level, showing differences in the effectiveness of ADM and IDM between expert and novice referees. Additionally, the study revealed that both expert and novice referees showed overconfidence, with experts demonstrating significantly higher overconfidence, particularly during IDM. In conclusion, the research highlighted the complexity of referees’ decision-making in high-pressure situations and emphasized the potential benefits of employing Analytical Decision-Making strategies. The study contributed to understanding cognitive biases in sports officiating and suggested the need for targeted training programs to help referees improve their performance and reduce overconfidence in challenging situations.

IntroductionThe notion of physical literacy is gaining interest from several countries as a potential mechanism for understanding the development of the physical self. This research endeavor represents an inaugural attempt to translate the Australian Physical Literacy Questionnaire for Children (PL-C Quest) into Chinese to evaluate the reliability and validity of the Chinese version of the PL-C Quest to assess physical literacy among children in mainland China.MethodsThe Beaton translation paradigm was used to carry out language translation, back-translation, cultural adaptation, and presurveys. Data were collected from 642 children aged 6–12 years, with a mean age of 9.71 years (SD 1.816), to test the reliability of the Chinese version of the PL-C Quest.ResultsThe PL-C Quest items translated well (6.187 ~ 15.499) and correlated well (0.441 ~ 0.622). The Chinese version of the PL-C Quest had good reliability, with retest reliability values ranging from 0.91 to 0.74, Cronbach’s alpha from 0.65 to 0.894, and McDonald’s ω from the Spearman-Brown Coefficient was 0.84. The validity results are acceptable because the CFI, IFI, and TLI values are above 0.8 and close to 0.9, but the model fit’s chi-square degrees-of-freedom ratio of 2.299, the RMSEA of 0.05, which was less than 0.08.DiscussionAfter translation and cultural adaptation, the Chinese version of the PL-C Quest is a reliable measurement tool and can be used in the Chinese region.

ObjectiveTo identify the research hotspots on cognitive function in developmental coordination disorder (DCD) in recent years, predict the research frontier and development trend, and provide more perspectives for the study of the DCD population.MethodsUsing CiteSpace and VOSviewer software to draw charts, 1,082 pieces of literature about DCD and cognitive function in the Web of Science core collection database from 2010 to 2022 were visually analyzed.Results and conclusionInterest in the cognitive function of DCD has been on the rise in the past 10 years. Over 40 countries and regions, 117 institutions and 200 researchers have participated in the corresponding research, mainly in the United States, and their institutions have published more highly influential results. The hot keywords are DCD, children, attention, working memory, performance, and attention-deficit/hyperactivity disorder (ADHD), and the main research hot topics include functional performance, population, cognitive psychology. The research directions include “DCD,” “Asperger syndrome,” “memory,” “infant,” “clumsiness,” “neurodevelopmental disorder,” “occupational therapy,” “preschool children,” “motor competence,” “model,” and “online control.” Future research should focus on motor imagery and intrinsic models and use more neurophysiological techniques to reveal the cognitive characteristics of children with DCD and develop intervention programs.

This cross-sectional study compared the physical fitness and mental health status of 140 school-age children who participated in sport-specific training with 180 age-matched peers. All the participants were grouped by sport-specific training frequencies in extracurricular time into the following: (i) high sports training frequency group (HFG): training three to five times per week (n = 77, mean [SD] age: 9.60 [0.12] years); (ii) low sports training frequency group (LFG): training once per week (n = 63, mean [SD] age: 9.88 [0.14] years); and (iii) control group (CG): maintaining routine life (n = 180, mean (SD) age: 9.77(0.09) years). Physical fitness status, including body composition (body mass index), endurance (vital capacity; 50 × 8 round trip), speed and agility (50 m sprint), flexibility (sit-and-reach), coordination (1-min rope skipping), and core strength (1-min sit-ups) as well as mental health status was measured. Overall, the results showed that Grade 3 to 4 HFG students showed better total physical fitness scores than the LFG and CG students. Grade 2 and 5 participants in the three groups showed no significant difference in the total physical fitness score. Children in HFG performed better in several PF indicators (i.e., cardiopulmonary function, flexibility, core strength, and coordination) than those in LFG and CG, and children in LFG got a higher score than those in CG on a testing item of 1-min rope skipping. The mental health test results showed that HFG performed better than LFG and CG. The results indicated that participating in sport-specific training 3–5 times per week was beneficial for children’s physical and mental health. Additionally, there was a weak and negative correlation between physical fitness and mental health in LFG and CG, while no correlation was found between physical fitness and mental health in HFG.

The purpose of this study is to investigate the effects of different types of acute exercise on cognitive function and cerebral oxygenation. A within-subject design was adopted. In total, 20 healthy older adults were enrolled in the study. They came to the laboratory individually on four separate days and completed four conditions of activity. Four conditions were sedentary reading control (RC), cognitive exercise (CE), physical exercise (PE) and cognitive + physical exercise (CE + PE). During these visits, participants completed the Stroop task before and immediately after the experimental condition, which consisted of 15 min of aerobic exercise, verbal fluency task (VFT), and dual task. The Stroop task included the following two conditions: a naming condition and an executive condition. The fNIRS is an optical method using near-infrared light to measure relative changes of oxygenated (O2Hb) and deoxygenated (HHb) hemoglobin in the cortex. The results indicate that acute exercise facilitates performance for executive tasks, not only combined cognition, but also the different results between combined exercise and single exercise. The fNIRS findings showed that acute single exercise influences oxygenation for executive tasks but not for naming tasks. Greater improvement was observed in the post-exercise session of combined exercise during the modified Stroop. These findings demonstrate that acute single exercise, single cognition exercise, and combined exercise enhanced the performance of the inhibition control task. Only acute combined exercise has a general facilitative effect on inhibition control. Combined exercise was shown to be superior to single exercise for task-efficient cerebral oxygenation and improved oxygen utilization during cortical activation in older individuals. Also, to maximize the performance of cognition it may be important for older adults to take part in more cognitive demand exercise or take more kinds of exercise.

Background Response inhibition is associated with successful sporting performance. However, research on response inhibition in athletes from open-skill sports has mainly focused on a consciously triggered variety; little is known about open-skill athletes’ response inhibition elicited by unconscious stimuli. Methods Here, we explored unconscious response inhibition differences between table tennis athletes (n = 20) and non-athletes (n = 19) using the masked go/no-go task and event-related potentials technique (ERPs). Results At the behavioral level, table tennis athletes displayed shorter go-response times (RTs) than non-athletes in the conscious condition. Furthermore, table tennis athletes exhibited longer response time–slowing (RT-slowing) than non-athletes in the unconscious condition. At the neural level, table tennis athletes displayed shorter event-related potential N2 component latencies than non-athletes for all conditions. More importantly, athletes displayed larger no-go event-related potential P3 component amplitudes than non-athletes at both the conscious and unconscious levels. Discussion The present study results suggested that table tennis athletes have superior conscious and unconscious response inhibition compared to non-athletes.

The purpose of this study was to investigate the benefits of exercises with different cognitive demands for cognitive functions (Executive and non-Executive) in healthy older adults. A cross-sectional design was adopted. In total, 84 healthy older adults were enrolled in the study. They were categorized into the Tai Chi group (TG), the brisk walking group (BG) or the control group (CG). Each participant performed the Stroop task and a digit comparison task. The Stroop task included the following three conditions: a naming condition, an inhibition condition and an executive condition. There were two experimental conditions in the digit comparison task: the non-delay condition and the delay condition. The results indicated that participants of the TG and BG revealed significant better performance than the CG in the executive condition of cognitive tasks and fitness. There was no significant difference of reaction time (RT) and accuracy rate in the inhibition and delay conditions of cognitive tasks and fitness between the TG and BG. The TG showed shorter reaction time in the naming and the executive conditions, and more accurate in the inhibition conditions than the BG. These findings demonstrated that regular participation in brisk walking and Tai Chi have significant beneficial effects on executive function and fitness. However, due to the high cognitive demands of the exercise, Tai Chi benefit cognitive functions (Executive and non-Executive) in older adults more than brisk walking does. Further studies should research the underlying mechanisms at the behavioural and neuroelectric levels, providing more evidence to explain the effect of high-cognitive demands exercise on different processing levels of cognition.

Abstract Background Cucurbita pepo cv Dayangua (CPD) is an edible plant with diverse pharmacological properties. The current research on CPD has primarily focused on initial investigations of its chemical composition and pharmacological effects, and no comprehensive toxicity assessment has been conducted to date. Methods In the present study, the toxicity of CPD was evaluated through both acute and sub-chronic oral toxicity tests in mice. 16S rDNA sequencing was used to analyze the composition of the gut microbiota of mice at different time points to observe the effect of CPD on these microbial communities. Results In the acute toxicity test, CPD exhibited low toxicity, with a median lethal dose (LD50) > 2000 mg/kg. The sub-chronic toxicity test indicated that CPD administration at doses of 200, 400, and 600 mg/kg did not cause mortality or significant organ damage in mice. Furthermore, analysis of the gut microbiota after gavage administration of CPD at 400 and 600 mg/kg revealed an improved abundance of some beneficial gut bacteria. Conclusions In summary, no acute or sub-chronic toxic effects were observed in mice following the oral administration of CPD. CPD did not affect the structure and diversity of the gut microbiota and may contribute to an increase in the number of beneficial gut bacteria.

Ca2+ activated BK channels are important for neural and muscle function. Here authors use a compound, BC5, to show that the interface between the cytosolic and voltage sensing domains is a major allosteric pathway for Ca2+ binding to open the channel.

Machine learning has played transformative roles in numerous chemical and biophysical problems such as protein folding where large amount of data exists. Nonetheless, many important problems remain challenging for data-driven machine learning approaches due to the limitation of data scarcity. One approach to overcome data scarcity is to incorporate physical principles such as through molecular modeling and simulation. Here, we focus on the big potassium (BK) channels that play important roles in cardiovascular and neural systems. Many mutants of BK channel are associated with various neurological and cardiovascular diseases, but the molecular effects are unknown. The voltage gating properties of BK channels have been characterized for 473 site-specific mutations experimentally over the last three decades; yet, these functional data by themselves remain far too sparse to derive a predictive model of BK channel voltage gating. Using physics-based modeling, we quantify the energetic effects of all single mutations on both open and closed states of the channel. Together with dynamic properties derived from atomistic simulations, these physical descriptors allow the training of random forest models that could reproduce unseen experimentally measured shifts in gating voltage, ∆V1/2, with a RMSE ~ 32 mV and correlation coefficient of R ~ 0.7. Importantly, the model appears capable of uncovering nontrivial physical principles underlying the gating of the channel, including a central role of hydrophobic gating. The model was further evaluated using four novel mutations of L235 and V236 on the S5 helix, mutations of which are predicted to have opposing effects on V1/2 and suggest a key role of S5 in mediating voltage sensor-pore coupling. The measured ∆V1/2 agree quantitatively with prediction for all four mutations, with a high correlation of R = 0.92 and RMSE = 18 mV. Therefore, the model can capture nontrivial voltage gating properties in regions where few mutations are known. The success of predictive modeling of BK voltage gating demonstrates the potential of combining physics and statistical learning for overcoming data scarcity in nontrivial protein function prediction.

A selective effect of motor expertise on mental rotation is revealed by a high correlation between the performance of sports experts and ability on a mental rotation task. Evidence is shown by studies involving a spatial factor, such as image interference or a movement constraint. Alternatively, the time constraint, as another performance factor, is considered critical in the effect of sport expertise on mental rotation. Three experiments were conducted to examine the role of time constraints in egocentric mental rotation and the stage performance of athletes (divers) and nonathletes. In Experiment 1, an egocentric mental rotation task in an untimed condition was conducted, and reaction times (RTs), error rates (ERs), RTs at 0 °, and mental rotation speed were assessed. The results indicated that divers outperformed nonathletes in terms of RT, as well as perceptual and decision stages and rotation stages. Experiments 2 and 3 added a relative time constraint (subtracting 1/2 SD of all the subjects' RT from the M of each group's RT) and an absolute time constraint (subtracting 1/2 SD of all the subjects' RT from the M of all the subjects' RT) to the task, respectively. Superior RT and lower ER were observed for the divers in the time constraint condition. Moreover, the results illustrated that divers were faster than nonathletes in both stages when facing time pressure. In general, the present study has, for the first time, confirmed the role of time in the relationship between sports expertise and mental rotation. [ABSTRACT FROM AUTHOR]

Chen and Jia investigate the synergistic regulating role of Ca2 + binding and the signaling lipid PIP2 in TMEM16A channel gating. Their study is significant as it provides new insights into the activated state of TMEM16A and highlights an example of functional importance of lipids in regulating membrane-associated proteins.

Fine identification and division of lithofacies types of continental shale strata is an important basis for the evaluation of shale gas exploration and development potential. At present, however, there is no consensus on the identification standard and division scheme of shale lithofacies. Taking the continental shale strata of the Middle–Lower Jurassic in the Sichuan Basin as an example, this paper established a lithofacies division method by means of core observation, whole-rock mineral X-ray diffraction analysis, thin section analysis, total organic carbon (TOC) measurement and helium porosity measurement after analyzing whole-rock mineral composition and shale characteristics. Then lithofacies types of shale strata were identified and divided, and characteristics of lithofacies assemblages in different scales were investigated. Finally, their significance for shale gas exploration was discussed. The following research results were obtained. First, 20 shale lithofacies types of 6 categories are totally identified in this continental shale strata using the newly established three-step lithofacies division method (whole-rock mineral composition partition–TOC classification–correction and improvement of mineral texture and sedimentary structure). Among them, medium–high TOC clay shale lithofacies, lamina–thin layer clay shale lithofacies and low–medium TOC silty shale lithofacies are dominant, followed by low–medium TOC shell limy clay shale lithofacies, and TOC bearing and low TOC silty clay shale lithofacies. Second, the average TOC and the average porosity of clay shale lithofacies and shell limestone clay shale lithofacies are higher than those of silty and silty clay shale lithofacies. It is indicated that mineral composition and lithofacies types of shale have a certain impact on gas source and reservoir performance. Third, three types of assemblages are identified in the continental shale strata, including mudstone–limestone assemblage, mudstone–sandstone assemblage and mudstone–limestone–sandstone mixed assemblage, which reflect the sedimentary characteristics of distal region, proximal region and transitional region in the lacustrine environment, respectively; and that the characterization of different lithofacies assemblages is conducive to recognizing the differences between different shale sedimentary environments. Fourth, fine identification and statistic of the number and frequency of limy shell laminae and thin layers in the terrestrial organic-rich shale with high clay mineral content can provide a basis for the fracturability evaluation of gas-rich zones and the optimization of optimum exploration and development intervals.

A model of a NUAA-PTRE pre-cooled air turbine engine was established. The design point parameters of the engine were optimized, including the pressure ratio, air flow rate of the compressor, efficiency, throat area, and efficiency of the turbine. The air flow rate at the engine operating point was 142.73 kg/s. High performance of the key components under a wide range of working conditions was realized after optimization. To achieve the indicators of the overall scheme, adaptability studies of key components were conducted. A three-stage variable geometry design was applied to the inlet. The pre-cooler was optimized with a power-to-weight ratio of over 100 kW/kg and a compactness of 278 m2/m3. The built-in rocket gas generator and dual-component injector were developed, and the combustion and heat transfer processes were simulated. The overall optimization design of the NUAA-PTRE and the adaptive design of the components were completed, and high performance of the engine in a wide range of flight conditions at Ma 0~5 and altitude 0~25 km was achieved.

The calcium-activated chloride channel (CaCC) TMEM16A plays crucial roles in regulating neuronal excitability and muscle contraction. Here authors show that phosphatidylinositol (4,5)-bisphosphate (PIP2) regulates TMEM16A channel activation and desensitization via binding to a putative binding site.

TMEM16F is an enigmatic Ca2 + -activated phospholipid scramblase (CaPLSase) that passively transports phospholipids. Here authors identify an inner activation gate and its disruption profoundly alters TMEM16F phospholipid permeation.

Large-conductance potassium (BK) channels are transmembrane (TM) proteins that can be synergistically and independently activated by membrane voltage and intracellular Ca2+. The only covalent connection between the cytosolic Ca2+ sensing domain and the TM pore and voltage sensing domains is a 15-residue ‘C-linker’. To determine the linker’s role in human BK activation, we designed a series of linker sequence scrambling mutants to suppress potential complex interplay of specific interactions with the rest of the protein. The results revealed a surprising sensitivity of BK activation to the linker sequence. Combining atomistic simulations and further mutagenesis experiments, we demonstrated that nonspecific interactions of the linker with membrane alone could directly modulate BK activation. The C-linker thus plays more direct roles in mediating allosteric coupling between BK domains than previously assumed. Our results suggest that covalent linkers could directly modulate TM protein function and should be considered an integral component of the sensing apparatus.

BK channels are regulated by membrane voltage and intracellular Ca2+ but the structural features that block the ion flow in the closed state remain unknown. Here authors use molecular dynamics simulation and show that a physical gate is not required; instead ion flow is regulated by hydrophobic dewetting due to changes in pore shape and surface hydrophobicity.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.