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Abstract Background Large spacers offer numerous advantages such as higher fusion rates and lower subsidence rates. However, due to the anatomical constraints of the approach, the use of large spacers in biportal endoscopic transforaminal lumbar interbody fusion(BE-TLIF) necessitates an additional incision and special instruments for spacer implantation leading to less frequent use. Methods This study has refined several techniques within BE-TLIF. We insert the cage and impact the cage transverse with a special design instrument in the same working portal. This allows for the use of large spacers during BE-TLIF procedures without the need for an auxiliary cage-inserting incision. Conclusion The technique is a straightforward, safe, and minimally invasive method for inserting large cages in the treatment of lumbar instability.

Spinal cord injury (SCI) is a traumatic condition that results in impaired motor and sensory function. Ferroptosis is one of the main causes of neural cell death and loss of neurological function in the spinal cord, and ferroptosis inhibitors are effective in reducing inflammation and repairing SCI. Although human umbilical cord mesenchymal stem cells (Huc-MSCs) can ameliorate inflammatory microenvironments and promote neural regeneration in SCI, their efficacy is greatly limited by the local microenvironment after SCI. Therefore, in this study, we constructed a drug-release nanoparticle system with synergistic Huc-MSCs and ferroptosis inhibitor, in which we anchored Huc-MSCs by a Tz-A6 peptide based on the CD44-targeting sequence, and combined with the reactive oxygen species (ROS)-responsive drug nanocarrier mPEG-b-Lys-BECI-TCO at the other end for SCI repair. Meanwhile, we also modified the classic ferroptosis inhibitor Ferrostatin-1 (Fer-1) and synthesized a new prodrug Feborastatin-1 (Feb-1). The results showed that this treatment regimen significantly inhibited the ferroptosis and inflammatory response after SCI, and promoted the recovery of neurological function in rats with SCI. This study developed a combination therapy for the treatment of SCI and also provides a new strategy for the construction of a drug-coordinated cell therapy system.

Abstract Hydrogels capable of swift mechanical energy dissipation hold promise for a range of applications including impact protection, shock absorption, and enhanced damage resistance. Traditional energy absorption in such materials typically relies on viscoelastic mechanisms, involving sacrificial bond breakage, yet often suffers from prolonged recovery times. Here, we introduce a hydrogel designed for friction-based damping. This hydrogel features an internal structure that facilitates the motion of a chain walker within its network, effectively dissipating mechanical stress. The hydrogel network architecture allows for rapid restoration of its damping capacity, often within seconds, ensuring swift material recovery post-deformation. We further demonstrate that this hydrogel can significantly shield encapsulated cells from mechanical trauma under repetitive compression, owing to its proficient energy damping and rapid rebound characteristics. Therefore, this hydrogel has potential for dynamic load applications like artificial muscles and synthetic cartilage, expanding the use of hydrogel dampers in biomechanics and related areas.

ABSTRACT: The prelaying period is critical for laying hens, marked by significant physiological changes such as increased egg production, hormone secretion, and higher nutritional demands. These changes stress the intestine, which is vital for nutrient digestion, absorption, immune defense, and maintaining antioxidant and microbial balance. During this period, maintaining the intestinal health is essential for efficient nutrient absorption. Curcumin, a plant-derived extract, offers antioxidant, anti-inflammatory, antimicrobial, and immunomodulatory benefits, that can improve gut function. This study evaluated the effects of curcumin on the intestinal structure, immune barrier function, and cecal microbiota composition in laying hens during their early laying period. A total of 180 Snowy White chickens (154 days old) were divided into 5 experimental groups, receiving curcumin at 0 mg/kg, 100 mg/kg, 200 mg/kg, 300 mg/kg, and 400 mg/kg for 12 wk. The results showed that curcumin significantly improved the intestinal morphology (P < 0.05), increased mRNA expression of digestive enzymes (such as MGAM, SI, and ANPEP), and enhanced the digestive and absorptive functions. Further, curcumin improved the levels of antioxidant parameters (such as CAT, GSH-PX, T-AOC, and T-SOD) in the ileum, jejunum, and duodenum, and increased the expression of immunoglobulins (IgA, IgM, IgG) in the intestinal segments and serum (P < 0.05). Curcumin also improved the intestinal immune barrier function by increasing ZO-1 and Occludin expression. Furthermore, it altered the gut microbiota composition by increasing the relative abundance of beneficial bacterial phyla such as Bacteroidetes, Actinobacteria, Proteobacteria, and Fusobacteria (P < 0.05). At the genus level, curcumin supplementation enhanced the beneficial genera like Phocaeicola, Alistipes, Prevotella, Barnesiella, and Bifidobacterium (P < 0.05), thereby promoting the gut health and microbial diversity. In conclusion, curcumin supplementation during the early laying period of hens offers significant benefits by improving the intestinal health, immune function, and gut microbiota composition. Hence, curcumin serves as a promising dietary additive to support nutrient absorption and immune defense in laying hens during the early laying period of hens.

The Middle Permian Maokou Formation in the Sichuan Basin is an important natural gas payzone. Recently, Zhejiang Oilfield Company of CNPC obtained industrial gas flow of vertical well test 42×103 m3/d and horizontal well (well DB 1H) test 556×103 m3/d in the limy source rock gas reservoir of eyelid and eyeball- shaped limestone in the first member of the Maokou Formation in well DB 1, Da'an exploration area, western Chongqing. A breakthrough has been made in the unconventional gas exploration of argillaceous limestone in the southern Sichuan and western Chongqing area, showing good exploration prospects in this area. The eyelid and eyeball-shaped limestone in the first member of the Maokou Formation in the Sichuan Basin has the characteristics of self-generation and self-storage, continuous and stable distribution and high thermal maturity. The limestone of central gentle slope facies shows no water sensitivity or acid sensitivity, and is dominated by carbonate brittle minerals, which is ideal for acid-fracturing development. It is of great significance to study the characteristics and main controlling factors of the organic rich limestone reservoir. Taking well DB 1 as an example, this paper carried out a study on the petrology, sedimentology and reservoir characteristics of the first member of the Maokou Formation, and it is found that, deposited on the central gentle slope with frequent water activity and abundant organism, the dark gray to black micrite with high organic matter content has good physical properties. In general, submember c has better physical properties than submember a. The development degree of macropores in eyelid-shaped limestone is higher than that in eyeball-shaped limestone, and the development degree of micropores is not much different. The main pore types of the first member of the Maokou Formation include dissolution pores, fractures, clay mineral apertures and organic pores. The pores in clay minerals (mainly talc) and organic matters are mainly distributed in the eyelid-shaped limestone. Depositional process and diagenesis are the main factors controlling the development of the eyelid and eyeball-shaped limestone reservoirs. The central gentle slope facies controls the development and distribution features of the organic-rich micrites. Diagenesis as well as fracture and dissolution effects improve the reservoir capacity of limestone, which is critical for hydrocarbon accumulation. Stable and high yield commercial gas flow was obtained in the vertical wells and horizontal wells in the first member of the Maokou Formation which has high organic matter content in well DB 1 block in the western Chongqing area, indicating that the accumulation and occurrence conditions of limestone source rock gas are good in the first member of the Maokou Formation. It has two kinds of accumulation modes with self-generation and self-storage dominance and short distance aggregation of local structural bands, and has the characteristics of continuous distribution of gas reservoir and large resource scale. The "continuous gas reservoir: in the limestone source rock in the first member of the Maokou Formation has great exploration potential, which will become an important exploration target with good potential in the Sichuan Basin.

Liquid-liquid phase separation (LLPS) is a captivating phenomenon in which a uniform mixture spontaneously divides into two liquid phases with differing component concentrations. It is prevalent in soft matter, is observed in systems involving polymers, organic molecules, and proteins, and is influenced by environmental factors and component properties. Recent recognition of LLPS within living organisms reveals its role in creating cellular compartments to orchestrate complex biochemical reactions, requiring distinct boundaries and unhindered molecular movement. Nonmembrane compartments, stemming from cytoplasmic LLPS, such as nucleoli, hold promise for synthetic cell engineering and cellular function insights. Under certain conditions, LLPS is linked to diseases such as sickle-cell disease, cancer, and neurodegenerative diseases. This review offers a concise overview of LLPS in soft matter, emphasizing its relevance in soft material engineering. We delve into fundamental mechanisms, focusing on biological systems, and explore the implications of LLPS, spanning organelles, substance exchange, molecular diffusion, and disease associations. LLPS enables soft material engineering, with applications in biomedicine and bioengineering, shaping future possibilities in bioengineering, from foundational cellular constructs to intricate artificial tissue development.

In an effort to enhance growth rates, chicken breeders have undertaken intensive genetic selection. In the selection process, the primary aim is to accelerate growth, inadvertently leading to new chicken breeds having an increased capacity for rapid adipose tissue accumulation. However, little is known about the relationship between changes in gene expression and adipose tissue accumulation and deposition in chickens. Therefore, in this study, RNA-seq analysis was utilized, and transcriptome data were obtained from the abdominal fat, thoracic subcutaneous fat, and clavicular fat on day 1 (d1), day 4, day 7, day 11, and day 15 to reveal the molecular mechanisms regulating the development and deposition of different adipose tissues in broiler chicks. The results showed that the key period for adipocyte differentiation and proliferation was between d4 and d7 (abdominal fat development) and between d1 and d4 (chest subcutaneous fat and clavicular fat). In addition, candidate genes such as MYOG, S100A9, CIDEC, THRSP, CXCL13, and NMU related to adipose tissue growth and development were identified. Further, genes (HOXC9, AGT, TMEM182, ANGPTL3, CRP, and DSG2) associated with the distribution of adipose tissue were identified, and genes (MN1, ANK2, and CAP2) related to adipose tissue growth were also identified. Taken together, the results from this study provide the basis for future studies on the mechanisms regulating adipose tissue development in chickens. Further, the candidate genes identified could be used in the selection process.

Spinal cord injury (SCI) is a debilitating condition that results in significant impairment of motor function and sensation. Despite the ongoing efforts to develop effective treatments, there are currently very limited options available for patients with SCI. Celastrol, a natural anti-inflammatory compound extracted from Tripterygium wilfordii, has been shown to exhibit anti-inflammatory and anti-apoptotic properties. In this study, we aimed to explore the therapeutic potential of celastrol for SCI and elucidate the underlying molecular mechanisms involved. We found that local tissue often experiences a significant decrease in cAMP content and occurrs apoptosis after SCI. However, the treatment of celastrol could promote the production of cAMP by up-regulating the VIP-ADCYAP1R1-GNAS pathway. This could effectively inhibit the phosphorylation of JNK and prevent apoptosis, ultimately improving the exercise ability after SCI. Together, our results reveal celastrol may be a promising therapeutic agent for the treatment of SCI.

Abstract Background CT-based abdominal skeletal muscle area (SMA) serves as a standard for assessing muscle mass in patients with cirrhosis. Few studies have used MR imaging to measure SMA in cirrhotic patients. The purpose of this study was to investigate the agreement and correlation of the SMA measured by MRI and CT in cirrhotic patients. Methods CT and MR images from 38 cirrhotic patients were analyzed using the Slice-O-Matic V5.0 software. One observer independently measured SMA at the mid-third lumbar vertebral (L3) level on CT and MR images. The intraclass correlation coefficient (ICC), Pearson correlation coefficient, and Bland–Altman plot were used to evaluate the agreement and correlation between CT and MRI SMA and their relationship with the sarcopenia severity and Child–Pugh grades. Results CT and MRI had a high intraobserver agreement, with ICCs ranging from 0.991 to 0.996. CT and MRI measurements were closely correlated (r = 0.991–0.998, all for P

Load-bearing biological tissues, such as cartilage and muscles, exhibit several crucial properties, including high elasticity, strength, and recoverability. These characteristics enable these tissues to endure significant mechanical stresses and swiftly recover after deformation, contributing to their exceptional durability and functionality. In contrast, while hydrogels are highly biocompatible and hold promise as synthetic biomaterials, their inherent network structure often limits their ability to simultaneously possess a diverse range of superior mechanical properties. As a result, the applications of hydrogels are significantly constrained. This article delves into the design mechanisms and mechanical properties of various tough hydrogels and investigates their applications in tissue engineering, flexible electronics, and other fields. The objective is to provide insights into the fabrication and application of hydrogels with combined high strength, stretchability, toughness, and fast recovery as well as their future development directions and challenges.

This paper proposes a nondestructive, separate transmitter-receiver (TX-RX) electromagnetic measurement system for near-surface detection. Different from the traditional dual-coil integrated design, the proposed transient electromagnetic (TEM) system performs shallow subsurface detection using independent TX coil and movable RX coils. This configuration requires a large primary field so that the far-away secondary field is able to generate reliably induced voltages. To achieve this goal, a bipolar current-pulsed power supply (BCPPS) with a late resonant charging strategy is designed to produce a sufficiently large magnetic moment for the exciting coil with low source interference. The magnetic dipole source (MDS) with a large proportion of weight is separated from the field observation device and does not need to be dragged or transported during the detection process. This setup lowers the weight of the scanning device to 3 kg and greatly improves the measurement efficiency. The results of the laboratory test verify the effectiveness of the separate MDS and RX module system. Field experimental detection further demonstrates that the proposed system can realize highly efficient and shallow surface detection within a 200 m range of the MDS device.

This topic prepared a composite nano-silver–tin slurry. According to the boiling point of the dispersant, 300 °C is determined as the sintering temperature. As the amount of tin added increases, the shear strength of Ag–Sn slurry gradually increases. When the amount of tin added is 1/20, the sintering strength reaches the maximum of 50 MPa. The increase in the shear strength is due to the strengthening of the second phase. When the amount of tin added exceeds 1/20, the sintering strength drops sharply, mainly due to the overgrowth of Ag3Sn, the product of the reaction of silver and tin. The porosity was measured accurately by Photoshop software. The porosities of pure silver, 5% tin, and 10% tin samples are 21.8%, 16.8%, and 12.5%, respectively. The Brinell hardness of the samples with pure silver and 5% tin is 20.60N/mm2 and 97.79N/mm2, respectively. After adding a small amount of nano-tin, due to its increased density, the porosity decreases, and the hardness value increases. Compared with nano-silver slurry, the sintering performance of nano-silver–tin slurry is improved. Therefore, it provides a certain reference for the preparation of nano-silver slurry.

Nonspecific interactions play a significant role in physiological activities, surface chemical modification, and artificial adhesives. However, nonspecificity sometimes causes sticky problems, including surface fouling, decreased target specificity, and artifacts in single-molecule measurements. Adjusting the liquid pH, using protein-blocking additives, adding nonionic surfactants, or increasing the salt concentration are common methods to minimize nonspecific binding to achieve high-quality data. Here, we report that grafting heteromorphic polyethylene glycol (Y-shape PEG) with two inert terminates could noticeably decrease nonspecific binding. As a proof-of-concept, we performed single-molecule force spectroscopy and fluorescence staining imaging experiments to verify the feasibility of Y-shape PEG in blocking nonspecific interactions. Our results indicate that Y-shape PEG could serve as a prominent and efficient candidate to minimize nonspecificity for scientific and biomedical applications.

Rotator cuff tears (RCTs) are common in shoulder disease and disability. Despite significant advances in surgical repair techniques, 20–70% of patients still have postoperative rotator cuff dysfunction. These functional defects may be related to retear or rotator cuff quality deterioration due to tendon retraction and scar tissue at the repair site. As an effective delivery system, hydrogel scaffolds may improve the healing of RCTs and be a useful treatment for irreparable rotator cuff injuries. Although many studies have tested this hypothesis, most are limited to laboratory animal experiments. This review summarizes differences in hydrogel scaffold construction, active ingredients, and application methods in recent research. Efforts to determine the indications of hydrogel scaffolds (with different constructions and cargos) for various types of RCTs, as well as the effectiveness and reliability of application methods and devices, are also discussed.

AbstractThe water-rich giant karst cave is the risk source of tunnel construction in the karst area. To reduce the risk of tunnel construction, it is necessary to accurately explore the spatial distribution range of the karst cave in the direction of advance and both sides of the tunnel. Reflection seismic and transient electromagnetic (TEM) are the primary geophysical tools for tunnel advanced prediction; they have strengths and weaknesses. The tunnel seismic can only describe the boundary interface between cave bodies and surrounding rock in the direction of advance. Although the TEM method can detect the spatial distribution range of cave bodies, the inversion results depend on the initial resistivity model. The single method is insufficient to describe the spatial distribution of cave bodies. To overcome the shortcoming of a single method, we developed tunnel seismic and TEM joint detection of karst cavern and established a complete data processing flow. The tunnel seismic migration profiles can describe the interface between the karst cave and surrounding rock in the direction of advance. We put forward a reasonable initial model: (1) the layer’s thicknesses are determined by the impedance interface from the tunnel migration data; (2) the initial resistivity values are determined from the pilot hole and prior geological data. Comparison of the inversion results of different initial models of tunnel face horizontal line data proves that the proposed initial model method can reduce multisolution, save calculation time, and improve inversion accuracy. The multidirectional TEM inversion profiles can describe the spatial distribution of the giant cave. Combined with the results of tunnel seismic and TEM, the interpretation errors can be reduced and the extension of the karst cave can be delineated. Later excavation results also verify the accuracy of the prediction results.

The Zhaotong National Shale Gas Demonstration Area along the southern margin of the Sichuan Basin is located in the complex marine tectonic area of South China, where shale deformation and reformation are intense and the factors controlling sweet spots are complex, so the preservation conditions have an important impact on the enrichment of shale gas. In order to support the selection and evaluation of shale gas sweet spots in this area and improve the success rate of drilling, this paper carried out a geological survey on field outcrops. Then, based on drilling, mud logging and physical property test data, the structural deformation pattern and the regional deformation characteristics of this demonstration area were analyzed, and the development characteristics of formation joints and fractures, the sealing capacity of shale surrounding rock and the distribution characteristics of gas reservoirs were studied. Finally, the preservation conditions of shale gas in the Wufeng Formation of Upper Ordovician and the Longmaxi Formation of Lower Silurian were discussed. And the following research results were obtained. First, in the Zhaotong National Shale Gas Demonstration Area, three structural deformation patterns are developed from south to north, including trough type, equal amplitude type and baffle type, which are distributed in three major deformation zones, respectively, i.e., the shear deformation zone of Central Guizhou Uplift, the compression–torsion deformation zone of northern Yunnan–Guizhou Depression and the compression deformation zone of Southern Sichuan Depression. Second, three types of joints and fractures whose relationships with the direction of strata are high angle, middle–low angle and bedding intersection are developed in the Wufeng–Longmaxi formations and its overlying strata, and their occurrence characteristics are basically consistent with those of the three major deformation zones. Third, the shale of Wufeng–Longmaxi formations is thick in the north and thin in the south and possesses the preservation conditions of source–reservoir integration and self-sealing hydrocarbon accumulation. And combined with the sealing ability of the overburden strata and the roof and floor, its preservation conditions are overall better. Fourth, from the perspective of shale gas component, this area can be divided into three belts, i.e., methane, methane + nitrogen mixture and nitrogen from north to south. And the preservation conditions of shale gas are generally better in the north and worse in the south. In conclusion, the shale in the central–northern part of Zhaotong National Shale Gas Demonstration Area (compression deformation area and its southern margin) is the most favorable area because of its large shale thickness, weak reformation and deformation, bedding development of joints and fractures, good sealing performance and excellent preservation conditions. The compression–torsion deformation zone of northern Yunnan–Guizhou Depression in the central part is moderate in preservation conditions, and it is the relatively favorable area. The shear deformation zone of Central Guizhou Uplift in the southern part has poor preservation conditions, and it is a prospective area.

Based on exploration and development results and evaluation of marine shale gas in South China in the past ten years, in view of the features of “high maturity, strong tectonic reformation and high shear stress” of the shale in Zhaotong exploration zone in the Yunnan and Guizhou Plateau, as well as the key issues of long time diffusion and leakage of shallow shale gas, and the preservation conditions, the factors controlling shallow shale gas sweet spot and key zone selection evaluation technology of shale gas are investigated. From 2017 to 2018, the first significant exploration breakthrough was made in the Taiyang anticline at a buried depth of 700 to 2 000 m, discovering large-scale proved geological reserves of shallow shale gas. By examining the accumulation conditions and sweet spot control factors of the shallow shale gas in this area, it is found that the accumulation and productivity potential of shale gas in the mountainous area with complex structure outside basin are controlled by five factors: (1) The gas-rich area has weak tectonic reformation and good preservation conditions on the whole, taking on typical anticline trap occurrence mode. (2) The gas-rich area is in over-pressure state and high in shale gas content. (3) The gas-rich area has high quality shale and thus superior source rock condition. (4) The gas-rich area has high quality reservoirs dominated by class I. (5) The shale gas reservoir in the gas-rich area has high content of brittle minerals and small difference between maximum and minimum horizontal stresses which are conducive to hydraulic fracturing. The innovative practice and core technologies formed during the exploration and production capacity construction of shallow shale gas in the Zhaotong demonstration zone have great reference significance for shallow shale gas exploration and development in other areas. Key words: shallow shale gas, Silurian Longmaxi Formation, Ordovician Wufeng Formation, exploration breakthrough, Taiyang anticline area, Zhaotong area, anticline

The transient electromagnetic (TEM) method is widely used in shallow surface engineering geological surveys due to its advantages such as light weight, high efficiency, and strong resolution. However, interpretation and inversion of TEM data is a complicated process. The traditional algorithm of TEM inversion employs the “smoke ring” fast imaging method, which can only reflect the approximate morphology of the stratigraphic model, and the inversion accuracy is low. Therefore, this method cannot meet the requirements of high-precision inversion. In this article, we present the particle swarm optimization (PSO) algorithm for TEM inversion. First of all, the response of the rectangular loop source TEM based on electric dipole integration was calculated and compared with the analytical solution results of the rectangular loop source and the accuracy of the algorithm was verified. Then, we introduced the solution process of the particle swarm optimization algorithm and analyzed the influence of particle swarm optimization algorithm parameter selection on the accuracy of the inversion result and the convergence speed. Next, a layered medium model was established. The particle swarm optimization algorithm and “smoke ring” fast imaging method were used to perform inversion calculation. The results show that the PSO algorithm has the advantages of high efficiency and accuracy. Finally, we examined the effectiveness of the particle swarm optimization algorithm for TEM data processing by inverting survey data from an Air-raid shelter on the campus of Chongqing University in China and comparing the results with those from the “smoke ring” fast imaging. The research works in this article provide new methods and techniques for TEM data processing.

Glue-type bio-adhesives are in high demand for many applications, including hemostasis, wound closure, and integration of bioelectronic devices, due to their injectable ability and in situ adhesion. However, most glue-type bio-adhesives cannot be used for short-term tissue adhesion due to their weak instant cohesion. Here, we show a novel glue-type bio-adhesive based on the phase separation of proteins and polysaccharides by functionalizing polysaccharides with dopa. The bio-adhesive exhibits increased adhesion performance and enhanced phase separation behaviors. Because of the cohesion from phase separation and adhesion from dopa, the bio-adhesive shows excellent instant and long-term adhesion performance for both organic and inorganic substrates. The long-term adhesion strength of the bio-glue on wet tissues reached 1.48 MPa (shear strength), while the interfacial toughness reached ~880 J m−2. Due to the unique phase separation behaviors, the bio-glue can even work normally in aqueous environments. At last, the feasibility of this glue-type bio-adhesive in the adhesion of various visceral tissues in vitro was demonstrated to have excellent biocompatibility. Given the convenience of application, biocompatibility, and robust bio-adhesion, we anticipate the bio-glue may find broad biomedical and clinical applications.

Implant-associated infections are major causes of implant failure. The development of antibacterial alloys with good biocompatibility and sufficient antibacterial properties are highly demanded. Herein, by employing Cu and Mo, we prepared a new Ti-12Mo-5Cu alloy with different heat treatments to control the size and distribution of Ti2Cu crystals. For the solution-treated sample at 750°C (SA750), Ti2Cu could undergo heterogeneous nucleation, whereas for the solution-treated sample at 900°C (SA900), nanoscale Ti2Cu crystals would be widely distributed throughout the alloy via homogeneous nucleation. Interestingly, SA900 presented better antibacterial effect than SA750, with the antibacterial mechanism proposed to be associated with the size and number of Ti2Cu in the obtained alloy. At evidenced by a rabbit osteomyelitis model, it was found that the new alloy had a better antibacterial effect than that of pure Ti. Compared to SA750, SA900 could further reduce the inflammatory reaction and slow the progression of osteomyelitis around the implant, then kept the cortical bone more intact. This study provides a straightforward strategy to control the precipitation of nanoscale Ti2Cu in Ti-12Mo-5Cu, obtaining a new antibacterial alloy with great biocompatibility, corrosion resistance and the ability to induce osteogenesis differentiation, promising for clinical translation.

In recent years, natural gas exploration in the Sinian Dengying Formation and shale gas exploration in Doushantuo Formation have made major breakthroughs in the Sichuan Basin and its adjacent areas. However, the sedimentary background of the Doushantuo Formation hasn't been studied systematically. The lithofacies paleogeographic pattern, sedimentary environment, sedimentary evolution and distribution of source rocks during the depositional stage of Doushantuo Formation were systematically analyzed by using a large amount of outcrop data, and a small amount of drilling and seismic data. (1) The sedimentary sequence and stratigraphic distribution of the Sinian Doushantuo Formation in the middle-upper Yangtze region were controlled by paleouplifts and marginal sags. The Doushantuo Formation in the paleouplift region was overlayed with thin thickness, including shore facies, mixed continental shelf facies and atypical carbonate platform facies. The marginal sag had complete strata and large thickness, and developed deep water shelf facies and restricted basin facies. (2) The Doushantuo Formation is divided into four members from bottom to top, and the sedimentary sequence is a complete sedimentary cycle of transgression–high position–regression. The first member is atypical carbonate gentle slope deposit in the early stage of the transgression, the second member is shore-mixed shelf deposit in the extensive transgression period, and the third member is atypical restricted–open sea platform deposit of the high position of the transgression. (3) The second member has organic-rich black shale developed with stable distribution and large thickness, which is an important source rock interval and major shale gas interval. The third member is characterized by microbial carbonate rock and has good storage conditions which is conducive to the accumulation of natural gas, phosphate and other mineral resources, so it is a new area worthy of attention. The Qinling trough and western Hubei trough are favorable areas for exploration of natural gas (including shale gas) and mineral resources such as phosphate and manganese ore. Key words: Sinian, Doushantuo Formation, lithofacies paleogeography, Sichuan Basin, paleouplift, marginal sag, carbonate platform, black shale, source rock

Chronic refractory wounds are one of the most serious complications of diabetes, and the effects of common treatments are limited. Chiral hydrogel combined with dimethyloxalyglycine (DMOG) as a dressing is a promising strategy for the treatment of chronic wounds. In this research, we have developed a DMOG-loaded supramolecular chiral amino-acid-derivative hydrogel for wound dressings for full-thickness skin regeneration of chronic wounds. The properties of the materials, the ability of sustained release drugs, and the ability to promote angiogenesis were tested in vitro, and the regeneration rate and repair ability of full-thickness skin were tested in vivo. The chiral hydrogel had the ability to release drugs slowly. It can effectively promote cell migration and angiogenesis in vitro, and promote full-thickness skin regeneration and angiogenesis in vivo. This work offers a new approach for repairing chronic wounds completely through a supramolecular chiral hydrogel loaded with DMOG.

This paper presents an improved empirical method for the prediction of perforation of steel plate reinforced concrete barriers by rigid missiles such as aircraft engines etc. The data of rigid missile experiments newly performed in China in 2018 were collected, and numerical analysis and existing empirical methods of perforation damage analysis were compared. Based on the existing formulas, an improvement is proposed to predict the penetration effect of typical rigid projectiles on steel reinforced concrete barriers in aircraft impact or tornadoes more accurately.

The exploration and development of marine shale gas reservoirs in South China is challenged by complex geological and geographical conditions, such as strong transformation, post maturity, complex mountains and humanity. In this paper, the evaluations on shale gas sweet spots conducted in Zhaotong demonstration zone in the past six years and the construction of 500 million m3 shale gas productivity in Huangjinba region were discussed, and the results of shale gas reservoir evaluations in China and abroad were investigated. Accordingly, it is proposed that another two key indicators be taken into consideration in the evaluation on shale gas sweet spots in marine mountains in South China, i.e. shale gas preservation conditions and pore pressure, and the research on ground stress and natural microfracture systems should be strengthened. Then, systematic analysis was conducted by integrating shale gas multidisciplinary data and geological and engineering integration study was carried out. Finally, a 3D model, which was composed of “geophysics, reservoir geology, fracture system and rock geomechanics”, was established for shale gas reservoirs. Application practice shows that the geological engineering integration and the 3D reservoir modeling are effective methods for evaluating the shale gas sweet spots in complex marine mountains in South China. Besides, based on shale gas sweet spot evaluation, 3D spatial congruency and superposition effects of multiple attributes and multiple evaluation parameters are presented. Moreover, the short-plate principle is the factor controlling the distribution patterns and evaluation results of shale gas sweet spots. It is concluded that this comprehensive evaluation method is innovative and effective in avoiding complex geological and engineering risks, so it is of guiding significance in exploration and development of marine shale gas in South China.

A transient electromagnetic detection method for site selection of substation grounding is proposed in this paper. And taking the fault detection experiment of transmission lines in a certain area of China as an example, the transient electromagnetic technology is used to obtain high-quality images reflecting the development trend and distribution of geological faults. The results show that the transient electromagnetic method is a convenient and effective way for substation site selection.

Metal coordination bonds are widely used as the dynamic cross-linkers to construct self-healing hydrogels. However, it remains challenging to independently improve the toughness of metal coordinated hydrogels without affecting the stretchability and self-healing properties, as all these features are directly correlated with the dynamic properties of the same metal coordination bonds. In this work, using histidine–Zn2+ binding as an example, we show that the coordination number (the number of binding sites in each cross-linking ligand) is an important parameter for the mechanical strength of the hydrogels. By increasing the coordination number of the binding site, the mechanical strength of the hydrogels can be greatly improved without sacrificing the stretchability and self-healing properties. By adjusting the peptide and Zn2+ concentrations, the hydrogels can achieve a set of demanding mechanical features, including the Young’s modulus of 7–123 kPa, fracture strain of 434–781%, toughness of 630–1350 kJ m−3, and self-healing time of ~1 h. We anticipate the engineered hydrogels can find broad applications in a variety of biomedical fields. Moreover, the concept of improving the mechanical strength of metal coordinated hydrogels by tuning the coordination number may inspire the design of other dynamically cross-linked hydrogels with further improved mechanical performance.

Women during pregnancy or puerperium are likely to develop Budd-Chiari syndrome (BCS). However, the reported prevalence of pregnancy-related BCS varied considerably among studies. Our study aims to systematically review this issue. Overall, 817 papers were initially identified via the PubMed, EMBASE, China National Knowledge Infrastructure, and Chinese Scientific and Technological Journal databases. Twenty of them were eligible. The prevalence of pregnancy-related BCS varied from 0% to 21.5%. The pooled prevalence was 6.8% (95% CI: 3.9–10.5%) in all BCS patients, 6.3% (95% CI: 3.8–9.4%) in primary BCS patients, and 13.1% (95% CI: 7.1–20.7%) in female BCS patients. Among them, one study was carried out in Africa with a prevalence of 10.6%; 14 studies in Asian countries with a pooled prevalence of 7.1% (95% CI: 3.1–12.6%); and 5 studies in European countries with a pooled prevalence of 5.0% (95% CI: 3.1–7.3%). The pooled prevalence was 6.7% (95% CI: 2.6–12.3%) in studies published before 2005 and 7.3% (95% CI: 4.2–12.5%) in those published after 2005. In conclusion, pregnancy is a relatively common risk factor for BCS, but there is a huge variation in the prevalence among studies. Physicians should be aware of pregnancy-related BCS.

Background: Osteomyelitis causes marked disability and is one of the most challenging diseases for orthopaedists to treat because of the considerable rate of infection recurrence. In this study, we proposed and assessed the debridementreconstruction-docking (DRD) systemfor the treatment of lower-extremity osteomyelitis. This procedure comprises 3 surgical stages and 2 preoperative assessments; namely, pre-debridement assessment, debridement, pre-reconstruction assessment, reconstruction, and docking-site management. We evaluated the use of the DRD system compared with the Ilizarov technique, which is defined as a 1-stage debridement, osteotomy, and bone transport. Methods: This retrospective cohort included 289 patients who underwent either DRD or the Ilizarov technique for the treatment of lower-extremity osteomyelitis at a single institution between January 2013 and February 2021 and who met the eligibility criteria. The primary outcome was the rate of infection recurrence. Secondary outcomes included the external fixator index (EFI), refracture rate, and the Paley classification for osseous and functional results. An inverse-probabilityweighted regression adjustment model was utilized to estimate the effect of the DRD system and Ilizarov technique on the treatment of lower-extremity osteomyelitis. Results: A total of 131 and 158 patients underwent DRD or the Ilizarov technique, respectively. The inverse-probabilityweighted regression adjustment model suggested that DRD was associated with a significant reduction in infection recurrence (risk ratio [RR], 0.26; 95% confidence interval [CI], 0.13 to 0.50; p < 0.001) and EFI (26.9 days/cm, 95% CI; 28.3 to25.5; p < 0.001). Patients in the DRD group had better Paley functional results than those in the Ilizarov group (ridit score, 0.55 versus 0.45; p < 0.001). There was no significant difference between the 2 groups in the rate of refracture (RR, 0.87; 95% CI, 0.42 to 1.79; p = 0.71) and Paley osseous results (ridit score, 0.51 versus 0.49; p = 0.39). Conclusions: In this balanced retrospective cohort of patients with lower-extremity osteomyelitis, the use of the DRD system was associated with a reduced rate of infection recurrence, a lower EFI, and better Paley functional results compared with the use of the Ilizarov technique. Level of Evidence: Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence. [ABSTRACT FROM AUTHOR]