63 results on '"Zhehu Jin"'
Search Results
2. Research progress on targeted drugs and its applications in the treatment of keloids
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Junzhi GAO, Yinli LUO, Longquan PI, and Zhehu JIN
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keloid ,targeted drug ,dupliumab ,nintedanib ,sunitinib ,sorafenib ,Dermatology ,RL1-803 - Abstract
Keloid (KD) is a pathological scar caused by excessive proliferation of fibroblasts and excessive deposition of extracellular matrix (ECM) in the skin during wound repair. It is often complicated with pain and pruritus, which can seriously affect the quality of life of patients. Although various treatments, such as injections, lasers, and surgeries, are available, the outcome is not satisfactory. Therefore, targeted therapeutic regimens, including a large molecule monoclonal antibody (Dupilumab) and the small molecule targeted drugs (Nintedanib, Sorafenib and Sunitinib), have been deployed to treat keloids. This review summarizes the progress on the treatment of keloids with these targeted drugs and their applications in the future.
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- 2024
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3. RUNX3 mediates keloid fibroblast proliferation through deacetylation of EZH2 by SIRT1
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Hanye Liu, Guanghai Yan, Li Li, Dandan Wang, Yu Wang, Shan Jin, Zhehu Jin, Liangchang Li, and Lianhua Zhu
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Keloid ,Abnormal proliferation ,Cell cycle ,SIRT1/EZH2/RUNX3 axis ,Cytology ,QH573-671 - Abstract
Abstract Background Keloid is a benign proliferative fibrous disease featured by excessive fibroblast proliferation after skin injury. However, the mechanism of abnormal cell proliferation is still unclear. Herein, we investigated the mechanism of abnormal proliferation in keloids involving Sirtuin 1(SIRT1)/ Zeste Homolog 2 (EZH2)/ Runt-related transcription factor 3 (RUNX3). Methods HE staining was used to observe the histopathological changes. Western blot was performed to detect SIRT1/EZH2/RUNX3 and cell cycle related proteins. RT-PCR detected EZH2 mRNA. After knockdown of EZH2 or overexpression of RUNX3, cell proliferation and cell cycle was analyzed. Immunoprecipitation was used to detect acetylated EZH2. Results The results showed that overexpression of RUNX3 inhibited cell proliferation and arrested cell cycle at G1/S phase, whereas inhibition of SIRT1 promoted cell proliferation and G1/S phase of the cell cycle. Knockdown of EZH2 promoted the expression of RUNX3, inhibited cell proliferation and shortened the progression of G1 to S phase. Simultaneous knockdown of EZH2 and inhibition of SIRT1 reversed these effects. Inhibition of SIRT1 increased its protein stability by increasing EZH2 acetylation, thereby reducing the expression of RUNX3 and promoting cell proliferation. Conclusions Conclusively, the SIRT1/EZH2/RUNX3 axis may be an important pathway in the regulation of abnormal proliferation in keloids.
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- 2022
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4. Integrated bioinformatics analysis of core regulatory elements involved in keloid formation
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Chuying Li, Meitong Jin, Yinli Luo, Zhehu Jin, and Longquan Pi
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Keloids ,Integrated bioinformatics analysis ,MicroRNAs ,Transcription factor ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Keloid is a benign fibro-proliferative dermal tumor formed by an abnormal scarring response to injury and characterized by excessive collagen accumulation and invasive growth. The mechanism of keloid formation has not been fully elucidated, especially during abnormal scarring. Here, we investigated the regulatory genes, micro-RNAs (miRNAs) and transcription factors (TFs) that influence keloid development by comparing keloid and normal scar as well as keloid and normal skin. Methods Gene expression profiles (GSE7890, GSE92566, GSE44270 and GSE3189) of 5 normal scar samples, 10 normal skin samples and 18 keloid samples from the Gene Expression Omnibus (GEO) database were interrogated. Differentially expressed genes (DEGs) were identified between keloid and normal skin samples as well as keloid and normal scar samples with R Project for Statistical Computing. Gene Ontology (GO) functional enrichment analysis was also performed with R software. DEG-associated protein–protein interaction (PPI) network was constructed by STRING, followed by module selection from the PPI network based on the MCODE analysis. Regulatory relationships between TF/miRNA and target genes were predicted with miRnet and cytoscape. Core regulatory genes were verified by RT-qPCR. Results We identified 628 DEGs, of which 626 were up-regulated and 2 were down-regulated. Seven core genes [neuropeptide Y(NPY), 5-hydroxytryptamine receptor 1A(HTR1A), somatostatin (SST), adenylate cyclase 8 (ADCY8), neuromedin U receptor 1 (NMUR1), G protein subunit gamma 3 (GNG3), and G protein subunit gamma 13 (GNG13)] all belong to MCODE1 and were enriched in the “G protein coupled receptor signaling pathway” of the GO biological process category. Furthermore, nine core miRNAs (hsa-mir-124, hsa-let-7, hsa-mir-155, hsa-mir-26a, hsa-mir-941, hsa-mir-10b, hsa-mir-20, hsa-mir-31 and hsa-mir-372), and two core TFs (SP1 and TERT) were identified to play important roles in keloid formation. In the TF/miRNA-target gene network, both hsa-mir-372 and hsa-mir-20 had a regulatory effect on GNG13, ADCY8 was predicted to be target by hsa-mir-10b, and HTR1A and NPY were potentially by SP1. Furthermore, the expression of core regulatory genes (GNG13, ADCY8, HTR1A and NPY) was validated in clinical samples. Conclusions GNG13, ADCY8, NPY and HTR1A may act as core genes in keloid formation and these core genes establish relationship with SP1 and miRNA (hsa-mir-372, hsa-mir-20, hsa-mir-10b), which may influence multiple signaling pathways in the pathogenesis of keloid.
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- 2021
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5. Poly amino acid thermosensitive hydrogel loaded with ICG-001 for inhibiting keloid by down-regulating the Wnt/β-Catenin pathway
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Fan Sheng, Yinli Luo, Di Wu, Jiachen Yuan, Ge Zheng, Yuhao Zheng, and Zhehu Jin
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Poly amino acid ,Thermosensitive hydrogel ,ICG-001 ,Keloid ,Wnt/β-Catenin pathway ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Keloids are benign tumors that appear as abnormal fibrous proliferations on the surface of the skin. A full understanding of the causation between key cells and molecules in the pathogenesis of scarring is required. This research aims to adopt the influence of the small molecule inhibitor ICG-001 of Wnt/β-catenin signaling pathway on the proliferation, invasion, and apoptosis of human keloid fibroblasts, and on the excessive deposition of collagen in human keloid fibroblasts, and the effect of on excessive collagen deposition and fibrosis in human keloid fibroblasts. It also aims to inject the polyamino acid thermosensitive hydrogel with contains ICG-001 drug around Keloid model in nude mice, exploring its inhibitory effect on tumor growth. The ICG-001 polyamino acid thermosensitive hydrogel material synthesized in this experiment can be injected into the vicinity of keloid and maintain the local drug concentration for a long time. As an inhibitor of Wnt/β-catenin signaling pathway, ICG-001 showed good effects of inhibiting the proliferation and promoting apoptosis of human keloid fibroblasts. The temperature-sensitive gel can be used to release the drug slowly, reducing the toxic and side effects of the drug. At the same time, because the material itself is non-toxic, this drug loaded gel has good therapeutic effect on keloids.
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- 2022
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6. LA67 Liposome-Loaded Thermo-Sensitive Hydrogel with Active Targeting for Efficient Treatment of Keloid via Peritumoral Injection
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Hongshuang Wan, Shuangqing Wang, Chuying Li, Bowen Zeng, Hao Wu, Chao Liu, Liqing Chen, Mingji Jin, Wei Huang, Yingda Zang, Dongming Zhang, Zhonggao Gao, and Zhehu Jin
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keloid ,in situ hydrogel ,pluronic ,triptolide ,Arg-Gly-Asp ,Pharmacy and materia medica ,RS1-441 - Abstract
A keloid is a benign tumor manifested as abnormal fibroplasia on the surface of the skin. Curing keloids has become a major clinical challenge, and searching for new treatments and medications has become critical. In this study, we developed a LA67 liposome-loaded thermo-sensitive hydrogel (LA67-RL-Gel) with active targeting for treating keloids via peritumoral injection and explored the anti-keloid mechanism. Firstly, Arg-Gly-Asp (RGD) peptide-modified liposomes (LA67-RL) loaded with LA67 were prepared with a particle size of 105.9 nm and a Zeta potential of −27.4 mV, and an encapsulation efficiency of 89.6 ± 3.7%. We then constructed a thermo-sensitive hydrogel loaded with LA67-RL by poloxamer 407 and 188. The formulation was optimized through the Box–Behnken design, where the impact of the proportion of the ingredients on the quality of the hydrogel was evaluated entirely. The optimal formulation was 20.7% P407 and 2.1% P188, and the gelation time at 37 °C was 9.5 s. LA67-RL-Gel slowly released 92.2 ± 0.8% of LA67 at pH 6.5 PBS for 72 h. LA67-RL-Gel increased adhesion with KF cells; increased uptake; promoted KF cells apoptosis; inhibited cell proliferation; reduced α-SMA content; decreased collagen I, collagen III, and fibronectin deposition; inhibited angiogenesis; and modulated the keloid microenvironment, ultimately exerting anti-keloid effects. In summary, this simple, low-cost, and highly effective anti-keloid liposome hydrogel provides a novel approach for treating keloids and deserves further development.
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- 2023
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7. Expert consensus on the use of omalizumab in chronic urticaria in China
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Zuotao Zhao, MD, PhD, Tao Cai, MD, Hong Chen, MD, Liuqing Chen, MD, Yudi Chen, MD, Xiang Gao, MD, Xinghua Gao, MD, Songmei Geng, MD, Yinshi Guo, MD, Fei Hao, MD, Guodong Hao, MM, Yan Hu, MD, Hongzhong Jin, MD, Zhehu Jin, MD, Chengxin Li, MD, Haili Li, MD, Jie Li, MD, Yanming Li, MD, Yunsheng Liang, MD, Guanghui Liu, MD, Qiang Liu, MD, Hai Long, MD, Lin Ma, MD, Yuanyuan Shang, MM, Yuxin Song, MB, Zhiqiang Song, MD, Xiangyang Su, MD, Haijing Sui, MD, Qing Sun, MD, Yuemei Sun, MB, Jianping Tang, MD, Xunliang Tong, MD, Huiying Wang, MD, Gang Wang, MD, Lianglu Wang, MD, Siqin Wang, MB, Li Xiang, MD, Ting Xiao, MD, Zhiqiang Xie, MD., Leping Ye, MD, Yongmei Yu, MM, Chunlei Zhang, MD, PhD, Litao Zhang, MD, Shuchen Zhang, MD, PhD, Rui Zheng, MD, Lili Zhi, MM, Wei Zhou, MD, Ying Zou, MD, and Marcus Maurer, MD
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Chronic spontaneous urticaria ,Chronic inducible urticaria ,Treatment algorithm ,Omalizumab ,China ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Chronic urticaria (CU) is a debilitating skin disease that lasts for more than 6 weeks with wheals and/or angioedema, including chronic spontaneous urticaria (CSU) and chronic inducible urticaria (CIndU). In China, the prevalence of this disease is high, more than 1%, and on the rise. CU has a major impact on the quality of life (QoL) of patients who frequently experience sleep disturbance, depression, and anxiety. Nearly one-third of patients with CSU, in China, are resistant to second-generation H1-antihistamines (sgAHs), even at a fourfold dose (second line; off-label). Omalizumab is approved for the treatment of CSU treatment in Europe and shows remarkable efficacy and safety. In China, regulatory approval for the use of omalizumab is pending, and its use in clinical practice varies widely. Consensus on omalizumab CU treatment in China is urgently needed. The aim of this article is to propose a practical omalizumab treatment algorithm for the management of antihistamine-resistant CSU and CIndU in adults and special population including children and adolescents, and pregnant or breast feeding women, to guide daily clinical practice in China. In the development of this consensus, an expert group including mainly dermatologists, allergists, but also pulmonologists, ENTs, immunologists, and pediatricians in Allergic Disease Prevention and Control Committee, Chinese Preventive Medicine Association, reviewed the existing evidence and developed consensus on the use of omalizumab in CU patients from China. The goal of this consensus is to assist clinicians in making rational decisions in the management of refractory CU with omalizumab. The key clinical questions covered by the treatment algorithm are: 1) Omalizumab treatment routine strategy in both CSU and CIndU patients; 2) Recommended dose and treatment duration for different age stratification; 3) Treatment duration for CU patients with other allergic comorbidities; 4) Recommendation on omalizumab stopping strategy.
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- 2021
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8. Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression
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Aili Cui, Zhehu Jin, Zhonggao Gao, Mingji Jin, Lianhua Zhu, Lianhua Li, Chenglong Jin, and Yinghua An
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Accumulating evidence indicated that aberrantly expressed microRNAs play critical roles in the initiation and progression of human cancers. However, the underlying functions of miR-493 in human melanoma remains unknown. Here, our study found that miR-493 expression was downregulated in human melanoma tissues and cells. Overexpression of miR-493 suppressed cell proliferation and cell cycle in human melanoma cell line A375. IRS4 was defined as a target for downregulation by miR-493 and was confirmed by luciferase assay. We also found that knockdown of IRS4 counteracted the proliferation promotion by miR-493 inhibitor. In summary, these results demonstrated that miR-493 acts as a tumor suppressor and inhibits cell proliferation and cell cycle in human melanoma by directly targeting IRS4.
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- 2017
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9. Mussel adhesive protein treatment delivered by microneedling for sensitive skin: A clinical study
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Richeng Dong, Qingmei Jin, Jiahui Zhi, Yinli Luo, Jiachen Yuan, Longquan Pi, Meilan Nan, Zhehu Jin, and Chenglong Jin
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Dermatology - Published
- 2023
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10. Implication of Amyloid Precursor-like Protein 2 Expression in Cutaneous Squamous Cell Carcinoma Pathogenesis.
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XIAODI HUANG, JIHYE YANG, HAORAN XI, MEILAN ZHANG, YEONGJOO OH, ZHEHU JIN, and ZHENLONG ZHENG
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AMYLOID beta-protein precursor ,PROTEIN expression ,SQUAMOUS cell carcinoma ,PATHOGENESIS ,MAJOR histocompatibility complex - Abstract
Background/Aim: Regulatory functions of amyloid precursor-like protein 2 (APLP2) expression in intracellular trafficking of major histocompatibility complex class I (MHC-I) and biological behavior of tumor cells have been reported in various types of malignancies but not in cutaneous squamous cell carcinoma (CSCC). This study aimed to investigate the role of APLP2 expression in the pathogenesis of CSCC. Patients and Methods: The expression of APLP2 and a key modulator of cancer immune escape, MHC-I, were determined in CSCC tissue samples obtained from 141 patients using immunohistochemistry. The regulatory effects of APLP2 expression on the biological behavior and surface expression of MHC-I in CSCC cells were investigated by trypan blue assay, Matrigel invasion assay, and in vivo xenograft analysis. Results: APLP2 immunoreactivity was high in 73 (51.8%) tissue samples from patients with CSCC and was significantly related to subcutaneous fat invasion and poor prognosis in our cohort. Moreover, proliferation of and invasion by CSCC cells were significantly reduced after APLP2 knockdown in CSCC cells both in vitro and in vivo. A significant association was found between APLP2 and membrane MHC-I expression in patients with CSCC. In vivo xenograft analysis showed that APLP2 knockdown increased membrane MHC-I expression in CSCC cells. Conclusion: APLP2 not only acts as an oncogene in CSCC progression but also as a possible modulator of cancer immune escape by influencing MHC-I expression on the cell surface. APLP2 may serve as a novel molecular biomarker and therapeutic target for patients with CSCC. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Novel technique for rosacea treatment using optimal pulse technology: In vivo and clinical studies
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Jiachen Yuan, Yang Gao, Longquan Pi, Zhouna Li, Meilan Nan, Zhehu Jin, and Chenglong Jin
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Dermatology - Abstract
Rosacea is a chronic inflammatory skin disease affecting the face, and the current treatment effect is not satisfactory. Based on the photomodulation of optimal pulse technology (OPT), we developed a novel treatment mode, namely, advanced OPT with low energy, three pulses, and long pulse width (AOPT-LTL).We aimed to explore the feasibility and underlying molecular mechanisms of AOPT-LTL treatment in a rosacea-like mouse model. Furthermore, we evaluated the safety and efficacy in patients with erythematotelangiectatic rosacea (ETR).Morphological, histological, and immunohistochemical analyses were used to investigate the efficacy and mechanisms of AOPT-LTL treatment in the LL-37-induced rosacea-like mouse model. Moreover, 23 patients with ETR were included and received different times of treatment at intervals of 2 weeks depending on the severity of their condition. The treatment effect was assessed by comparing clinical photographs at baseline, 1 week, and 3 months after treatment, combined with the red value, GFSS, and CEA scores.After the AOPT-LTL treatment of the mice, we observed that the rosacea-like phenotype, inflammatory cell infiltration, and vascular abnormalities were significantly ameliorated, and the expression of the core molecules of rosacea was significantly inhibited. In the clinical study, the AOPT-LTL treatment exerted satisfactory therapeutic effects on erythema and flushing of ETR patients. No serious adverse events were observed.AOPT-LTL is a safe and effective method for the treatment of ETR.
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- 2022
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12. Adipose‐derived mesenchymal stem cells‐sourced exosomal micro <scp>RNA</scp> ‐7846‐3p suppresses proliferation and pro‐angiogenic role of keloid fibroblasts by suppressing neuropilin 2
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Di Wu, Xiao Liu, and Zhehu Jin
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Dermatology - Published
- 2023
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13. Oleanolic acid regulates the proliferation and extracellular matrix of keloid fibroblasts by mediating the <scp>TGF</scp> ‐β1/ <scp>SMAD</scp> signaling pathway
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Yinli Luo, Dongming Wang, Xinghua Yuan, Zhehu Jin, and Longquan Pi
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Dermatology - Published
- 2023
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14. Proteasome Subunit Alpha Type-7 Expression Suppresses Cutaneous Squamous Cell Carcinoma Progression by Inhibiting Cancer-associated Cytokines.
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XIANGSHU XU, MEILING PEI, KI-YEOL KIM, HAORAN XI, SANG GYUN LEE, KEE YANG CHUNG, MI RYUNG ROH, and ZHEHU JIN
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PROTEASOMES ,SQUAMOUS cell carcinoma ,DISEASE progression ,CYTOKINES ,ANTIVIRAL agents - Abstract
Background/Aim: Cutaneous squamous cell carcinoma (cSCC) is a common non-melanoma skin cancer, and its incidence is increasing. Proteasome subunit alpha type-7 (PSMA7) has been found to be aberrantly expressed in several cancers. However, whether it functions as a tumor suppressor or oncogene in the pathogenesis of cancers, particularly cSCC, remains controversial. Here, we aimed to investigate the functions of PSMA7 in cSCC pathogenesis. Patients and Methods: Clinicopathological characteristics were evaluated in 131 patients with cSCC using tissue sections. The expression of PSMA7, nucleotide-binding oligomerization domain-containing protein 1 (NOD1), and mitochondrial antiviral signaling protein (MAVS) was determined in cSCC tissue sections using immunohistochemical staining. The effect of PSMA7 expression on the biological behavior of cSCC cells was investigated in vitro. Results: High immunoreactivity of PSMA7 (high-PSMA7) was detected in 53 (40.5%) patients with cSCC and was significantly associated with histologic grade (p=0.008) and favorable recurrence-free survival (p=0.018). The expression of PSMA7 and NOD1 (p=0.026) and MAVS (p=0.032) was negatively correlated in cSCC tissues. Contrary to the results of the cohort study, cell viability and invasiveness significantly decreased after PSMA7 down-regulation in cSCC cells in vitro. mRNA expression of tumor necrosis factor-alpha, interleukin-1 alpha (IL-1a), IL-6, and IL-8 were significantly increased after PSMA7 down-regulation in cSCC cells (all p=0.002). Conclusion: PSMA7-mediated degradation of NOD1 and MAVS as well as the subsequent reduction of the cancer-associated cytokine network may be a crucial mechanism of the antitumoral function of PSMA7 in patients with cSCC. [ABSTRACT FROM AUTHOR]
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- 2023
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15. Glaucocalyxin A Attenuates Allergic Responses by Inhibiting Mast Cell Degranulation through p38MAPK/NrF2/HO-1 and HMGB1/TLR4/NF-κB Signaling Pathways
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Shan Jin, Chongyang Wang, Zhiguang Wang, Li Li, Zhehu Jin, Liangchang Li, Jingzhi Jiang, Yihua Piao, Guanghai Yan, Hongmei Piao, and Lianhua Zhu
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0303 health sciences ,Article Subject ,medicine.medical_treatment ,Degranulation ,Pharmacology ,Calcium in biology ,Allergic inflammation ,Other systems of medicine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cytokine ,Complementary and alternative medicine ,chemistry ,LYN ,030220 oncology & carcinogenesis ,medicine ,Phosphorylation ,lipids (amino acids, peptides, and proteins) ,Signal transduction ,RZ201-999 ,Histamine ,030304 developmental biology - Abstract
Glaucocalyxin A (GLA) has various pharmacological effects like antioxidation, immune regulation, and antiatherosclerosis. Here, in this study, the effect and mechanism of GLA on mast cell degranulation were studied. The results of the anti-DNP IgE-mediated passive cutaneous anaphylaxis (PCA) showed that GLA dramatically inhibited PCA in vivo, as evidenced by reduced Evans blue extravasation and decreased ear thickness. In addition, GLA significantly reduced the release of histamine and β-hexosaminidase, calcium influx, cytokine (IL-4, TNF-α, IL-1β, IL-13, and IL-8) production in the RBL-2H3 (rat basophilic leukemia cells), and RPMCs (peritoneal mast cells) in vitro. Moreover, we further investigated the regulatory mechanism of GLA on antigen-induced mast cells by Western blot, which showed that GLA inhibited FcεRI-mediated signal transduction and invalidated the phosphorylation of Syk, Fyn, Lyn, Gab2, and PLC-γ1. In addition, GLA inhibited the recombinant mouse high mobility group protein B1- (HMGB1-) induced mast cell degranulation through limiting nuclear translocation of NF-κBp65. Treatment of mast cells with siRNA-HMGB1 significantly inhibited HMGB1 levels, as well as MyD88 and TLR4, decreased intracellular calcium levels, and suppressed the release of β-hexosaminidase. Meanwhile, GLA increased NrF2 and HO-1 levels by activating p38MAPK phosphorylation. Consequently, these data suggest that GLA regulates the NrF2/HO-1 signaling pathway through p38MAPK phosphorylation and inhibits HMGB1/TLR4/NF-κB signaling pathway to reduce mast cell degranulation and allergic inflammation. Our findings could be used as a promising therapeutic drug against allergic inflammatory disease.
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- 2021
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16. A novel technique for treating atrophic facial scars in Asians using ultra‐pulse CO 2 laser
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Chenglong Jin, Zhouna Li, Wenyan Jin, and Zhehu Jin
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Novel technique ,medicine.medical_specialty ,Erythema ,business.industry ,Pulse (signal processing) ,Atrophic scars ,Scars ,Dermatology ,Thermal contraction ,Surgery ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Co 2 laser ,030220 oncology & carcinogenesis ,medicine ,Clinical efficacy ,medicine.symptom ,business - Abstract
Background and aims Fractional lasers have become increasingly popular for treating atrophic scars, but their effectiveness is limited for deeper scars. We developed a novel technique (manual fractional thermal contraction technology, MFTCT) using an ultra-pulse CO2 laser and evaluated its efficacy and safety for treating atrophic facial scars. Methods A total of 44 patients with atrophic facial scars were treated with MFTCT every 8 weeks for 1-4 times. Overall scar improvement was assessed by photographs taken at baseline and 3 months after the last treatment according to the 4-point global assessment scale (GAS) and ECCA grading scale. Improvements in color, distortion, and texture were assessed by the modified Manchester Scar Scale and scored individually from 1 to 4. Pain degrees and adverse reactions during and after treatment were recorded. Results A total of 44 patients completed the treatment and follow-ups; of them, 89% reported at least 50% overall improvement after the last treatment. The mean ECCA scores fell from 67.50 ± 23.98 to 45.68 ± 18.57 (a 32% improvement), and the change was significant (P = .000). The average score for overall improvement was 3.48. The average scores for color, distortion, and texture were 3.07 ± 0.62, 3.27 ± 0.50, and 3.52 ± 0.51, respectively. Mean pain degree score was 4.27 ± 1.04, and mean erythema duration was 28.43 ± 6.58 days. Some patients developed pigmentation for a few months that resolved with topical treatment. Conclusion Manual fractional thermal contraction technology has definite clinical efficacy in the treatment of atrophic facial scars with fewer adverse reactions and is worth using in the clinical setting.
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- 2020
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17. Effective of a novel technique for sensitive skin treatment with optimal pulse technology: A clinical study
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Jiachen Yuan, Yang Gao, Zhouna Li, Meilan Nan, Jian Liu, Zhehu Jin, and Chenglong Jin
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Treatment Outcome ,Face ,Humans ,Female ,Skin Pigmentation ,Dermatology ,Administration, Cutaneous ,Skin Aging ,Skin - Abstract
Photomodulation is a non-photothermal effect that mobilizes energy and regulates cell activity at the mitochondrial level, and has been used to treat sensitive skin (SS) in recent years. Based on the photomodulated effect of optimal pulse technology (OPT), we developed a novel treatment mode (advanced OPT with low energy, three pulses, long pulse width, AOPT-LTL) for the treatment of facial SS and evaluated its effectiveness.A total of thirty Chinese women with SS were included in this study. Patients were received different times of AOPT-LTL treatment with an interval of 2 to 4 weeks depending on the severity. Clinical improvement was evaluated by comparing baseline and post-treatment photographs. In addition, the skin objective signs and subjective symptoms, as well as adverse events and patient satisfaction were also analyzed and tabulated.All included patients completed the treatment and follow-up period. After one course of treatment, 76.7% of patients had a Symptom Score Reducing Index (SSRI)20%, suggesting that the treatment was effective. Within two courses of treatment, all patients had SSRI20%, demonstrating significant improvement in skin sensitivity. The analysis of clinical photographs showed that facial dryness, desquamation, flushing, and skin color significantly improved, capillary density decreased, the dilated capillaries were retracted. During the treatment period, no obvious adverse reactions occurred in any patients, and the patients' satisfaction was high.This novel technique of AOPT-LTL might be an effective and safe modality for the treatment of SS.
- Published
- 2021
18. Second intention healing of nasal ala and dorsum defects in Asians
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Chenglong Jin, Zhehu Jin, Zhouna Li, Wenyan Jin, and Shan Jin
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Adult ,Male ,Dorsum ,medicine.medical_specialty ,Skin Neoplasms ,Dermatology ,Nose ,Young Adult ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Re-Epithelialization ,medicine ,Humans ,Child ,Retrospective Studies ,Skin ,030203 arthritis & rheumatology ,Wound Healing ,business.industry ,Middle Aged ,Surgery ,Nasal ala ,Female ,Surgical excision ,business - Abstract
Reconstruction of defects of nasal ala and dorsum after surgical excision presents a substantial challenge to dermatologic surgeons. Second intention healing is a simple and extremely useful method to optimize cosmesis after skin cancer removal.This study reported the cosmetic outcomes after second intention healing of nasal ala and dorsum defects in Asians, and estimated the time to epithelialization and complete healing.Fifteen defects (1 cm in diameter) of the nasal ala and dorsum in 10 patients were allowed to heal by secondary intention. Cosmetic results were evaluated and the time to epithelialization and complete healing were recorded.Cosmetic outcomes were good to excellent in 80% of the defects; defects of the dorsum showed poorer cosmetic results than defects of the ala. The wounds needed 5-17 days (mean 11.3; SD ± 4.18) to complete epithelialization and 10-24 days (mean 17.7; SD ± 4.85) to heal completely.Second intention healing of small nasal ala and dorsum defects (1 cm in diameter) in Asians produces satisfactory cosmetic results with a low complication rate.
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- 2019
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19. Improving the anti-keloid outcomes through liposomes loading paclitaxel–cholesterol complexes
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Wei Huang, Qiming Wang, Zhehu Jin, Mengjiao Wang, Zhonggao Gao, Liqing Chen, and Mingji Jin
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Biophysics ,Pharmaceutical Science ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biomaterials ,chemistry.chemical_compound ,Keloid ,In vivo ,Drug Discovery ,medicine ,LY294002 ,Protein kinase B ,GSK3B ,PI3K/AKT/mTOR pathway ,biology ,Organic Chemistry ,General Medicine ,Transforming growth factor beta ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,chemistry ,Paclitaxel ,biology.protein ,Cancer research ,0210 nano-technology - Abstract
Improving the anti-keloid outcomes through liposomes loading paclitaxel-cholesterol complexes Mengjiao Wang,1 Liqing Chen,2 Wei Huang,2 Mingji Jin,2 Qiming Wang,2 Zhonggao Gao,2 Zhehu Jin1 1Klebs Research Center, Department of Dermatology, Yanbian University Hospital, Yanji 133000, China; 2State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China Background: Keloids represent benign fibroproliferative tumors which result from elevated expression of inflammation. Paclitaxel (PTX) was an effective chemotherapeutic agent and has been reported to have anti-fibrotic effects, but the strong hydrophobicity brings a challenge for its clinical application. Purpose: The objective of this study was to improve the water solubility of PTX and investigate its anti-keloid effects. Methods: We prepared a PTX-cholesterol-loaded liposomes (PTXL) by thin film evaporation fashion and characterized their physicochemical properties. We also investigated the effects of PTX on proliferation, invasion and fibrosis of keloid fibroblasts in vitro and in vivo. Results: The prepared PTXL have a spherical appearance, a particle size of 101.43 nm and a zeta potential of -41.63 mV. PTXL possessed a high drug entrapment efficiency of 95.63% and exhibited a good stability within 30 days. The drugs in PTXL were released in a slow and sustained mode. The PTXL could be effectively uptaken into human keloids fibroblast (HKFs) in a time-dependent manner. In vitro, PTXL showed better ability on inhibiting cell proliferation, migration and invasion, and effectively on promoting apoptosis and arresting cell cycle in G2/M phase compared to PTX. Meanwhile, in vivo studies indicated that the PTXL had better performance on inhibiting the keloids growth compared to the PTX in keloid-bearing BALB/c nude mice model. Finally, we found PTX treatment suppressed the production of tumor necrosis factor alpah (TNF-α), interleukin 6 (IL-6) and transforming growth factor beta (TGF-β) and inhibited the expression of alpha smooth muscle actin (β-SMA) and collagen I in HKFs. The activation of protein kinase B (AKT)/glycogen synthase kinase 3 beta (GSK3β) signaling pathway also blocked by PTX in cultured HKFs and keloid tissues. LY294002, a PI3K (phosphatidylinositol 3-kinase)/AKT inhibitor, also suppressed the expression of TNF-α, IL-6 and TGF-β, and simultaneously, reduced the production of α-SMA and collagen I in HKFs. The inhibition of AKT/GSK3β signaling pathway contribute to inhibit the generation of fibrogenic cytokines by PTXL on ameliorating fibrosis progress in keloids. Conclusion: Our results suggested that the developed PTXL would become a promising therapeutic agent in the field of anti-keloid therapy. Keywords: keloids, paclitaxel, liposomes, AKT, GSK3β, fibrosis
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- 2019
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20. Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
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Liangchang Li, Guanghai Yan, Hongmei Li, Mingyu Zheng, Zhemin Xian, Lianhua Zhu, Zhehu Jin, Guangyu Jin, Hongmei Piao, Jingzhi Jiang, and Chongyang Wang
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MAPK/ERK pathway ,Article Subject ,lcsh:Medicine ,Syk ,GAB2 ,Pharmacology ,Cell Degranulation ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,Proinflammatory cytokine ,Rats, Sprague-Dawley ,Mice ,Phosphatidylinositol 3-Kinases ,LYN ,Furocoumarins ,Hypersensitivity ,Animals ,Syk Kinase ,Mast Cells ,Phosphorylation ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Mice, Inbred BALB C ,General Immunology and Microbiology ,biology ,Phospholipase C gamma ,Tumor Necrosis Factor-alpha ,Chemistry ,Passive Cutaneous Anaphylaxis ,lcsh:R ,Intracellular Signaling Peptides and Proteins ,NF-kappa B ,Degranulation ,General Medicine ,Immunoglobulin E ,Protein-Tyrosine Kinases ,enzymes and coenzymes (carbohydrates) ,biology.protein ,Cytokines ,Female ,Signal Transduction ,Research Article - Abstract
This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were used to detect the levels of corresponding cytokines and signalling pathway proteins. IMP decreased the leakage of Evans blue and the ear thickness in the PCA models, in a dose-dependent manner, and alleviated the degranulation of mast cells. Moreover, IMP reduced the expression of TNF-α, IL-4, IL-1β, IL-8, and IL-13. Furthermore, IMP inhibited the phosphorylation levels of Syk, Lyn, PLC-γ1, and Gab2, as well as the downstream MAPK, PI3K/AKT, and NF-κB signaling pathways. In addition, IMP inhibited the mast cell-mediated allergic responses through the Nrf2/HO-1 pathway. IMP attenuates the allergic responses through inhibiting the degranulation and decreasing the expression levels of proinflammatory cytokines in the mast cells, involving the PI3K/Akt, MAPK, NF-κB, and Nrf2/HO-1 pathways.
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- 2019
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21. Glaucocalyxin A Attenuates Allergic Responses by Inhibiting Mast Cell Degranulation through p38MAPK/NrF2/HO-1 and HMGB1/TLR4/NF
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Yihua, Piao, Jingzhi, Jiang, Zhiguang, Wang, Chongyang, Wang, Shan, Jin, Li, Li, Liangchang, Li, Hongmei, Piao, Zhehu, Jin, Lianhua, Zhu, and Guanghai, Yan
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lipids (amino acids, peptides, and proteins) ,Research Article - Abstract
Glaucocalyxin A (GLA) has various pharmacological effects like antioxidation, immune regulation, and antiatherosclerosis. Here, in this study, the effect and mechanism of GLA on mast cell degranulation were studied. The results of the anti-DNP IgE-mediated passive cutaneous anaphylaxis (PCA) showed that GLA dramatically inhibited PCA in vivo, as evidenced by reduced Evans blue extravasation and decreased ear thickness. In addition, GLA significantly reduced the release of histamine and β-hexosaminidase, calcium influx, cytokine (IL-4, TNF-α, IL-1β, IL-13, and IL-8) production in the RBL-2H3 (rat basophilic leukemia cells), and RPMCs (peritoneal mast cells) in vitro. Moreover, we further investigated the regulatory mechanism of GLA on antigen-induced mast cells by Western blot, which showed that GLA inhibited FcεRI-mediated signal transduction and invalidated the phosphorylation of Syk, Fyn, Lyn, Gab2, and PLC-γ1. In addition, GLA inhibited the recombinant mouse high mobility group protein B1- (HMGB1-) induced mast cell degranulation through limiting nuclear translocation of NF-κBp65. Treatment of mast cells with siRNA-HMGB1 significantly inhibited HMGB1 levels, as well as MyD88 and TLR4, decreased intracellular calcium levels, and suppressed the release of β-hexosaminidase. Meanwhile, GLA increased NrF2 and HO-1 levels by activating p38MAPK phosphorylation. Consequently, these data suggest that GLA regulates the NrF2/HO-1 signaling pathway through p38MAPK phosphorylation and inhibits HMGB1/TLR4/NF-κB signaling pathway to reduce mast cell degranulation and allergic inflammation. Our findings could be used as a promising therapeutic drug against allergic inflammatory disease.
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- 2020
22. Effects of G-Rh2 on mast cell-mediated anaphylaxis via AKT-Nrf2/NF-κB and MAPK-Nrf2/NF-κB pathways
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Chongyang Wang, Guanghai Yan, Lianhua Zhu, Liangchang Li, Zhehu Jin, Shan Jin, Jung Joon Lee, Guanhao Li, Jingzhi Jiang, Li Li, and Chang Xu
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biology ,medicine.medical_treatment ,Degranulation ,Syk ,respiratory system ,Pharmacology ,Immunoglobulin E ,Mast cell ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Allergic inflammation ,chemistry.chemical_compound ,Cytokine ,medicine.anatomical_structure ,Complementary and alternative medicine ,chemistry ,LYN ,medicine ,biology.protein ,Histamine ,Biotechnology - Abstract
Background The effect of ginsenoside Rh2 (G-Rh2) on mast cell-mediated anaphylaxis remains unclear. Herein, we investigated the effects of G-Rh2 on OVA-induced asthmatic mice and on mast cell-mediated anaphylaxis. Methods Asthma model was established for evaluating airway changes and ear allergy. RPMCs and RBL-2H3 were used for in vitro experiments. Calcium uptake, histamine release and degranulation were detected. ELISA and Western blot measured cytokine and protein levels, respectively. Results G-Rh2 inhibited OVA-induced airway remodeling, the production of TNF-α, IL-4, IL-8, IL-1β and the degranulation of mast cells of asthmatic mice. G-Rh2 inhibited the activation of Syk and Lyn in lung tissue of OVA-induced asthmatic mice. G-Rh2 inhibited serum IgE production in OVA induced asthmatic mice. Furthermore, G-Rh2 reduced the ear allergy in IgE-sensitized mice. G-Rh2 decreased the ear thickness. In vitro experiments G-Rh2 significantly reduced calcium uptake and inhibited histamine release and degranulation in RPMCs. In addition, G-Rh2 reduced the production of IL-1β, TNF-α, IL-8, and IL-4 in IgE-sensitized RBL-2H3 cells. Interestingly, G-Rh2 was involved in the FceRI pathway activation of mast cells and the transduction of the Lyn/Syk signaling pathway. G-Rh2 inhibited PI3K activity in a dose-dependent manner. By blocking the antigen-induced phosphorylation of Lyn, Syk, LAT, PLCγ2, PI3K ERK1/2 and Raf-1 expression, G-Rh2 inhibited the NF-κB, AKT-Nrf2, and p38MAPK-Nrf2 pathways. However, G-Rh2 up-regulated Keap-1 expression. Meanwhile, G-Rh2 reduced the levels of p-AKT, p38MAPK and Nrf2 in RBL-2H3 sensitized IgE cells and inhibited NF-κB signaling pathway activation by activating the AKT-Nrf2 and p38MAPK-Nrf2 pathways. Conclusion G-Rh2 inhibits mast cell-induced allergic inflammation, which might be mediated by the AKT-Nrf2/NF-κB and p38MAPK-Nrf2/NF-κB signaling pathways.
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- 2020
23. Inhibitory effect of fenretinide-loaded liposomes on subcutaneous transplanted tumors in nude mice inoculated with A375 melanoma cells: a preliminary study
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Zhehu Jin, Wenyan Jin, and Aili Cui
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Nevi and melanomas ,biology ,business.industry ,Melanoma ,Spleen ,Dermatology ,biology.organism_classification ,medicine.disease ,Metastasis ,Staining ,Andrology ,Infectious Diseases ,medicine.anatomical_structure ,Nude mouse ,Apoptosis ,Medicine ,Immunohistochemistry ,business - Abstract
Objective To evaluate the inhibitory effect of fenretinide-loaded liposomes (4-HPR-L) on subcutaneous transplanted malignant melanomas in nude mice. Methods A film-ultrasonic dispersion method was used to prepare 4-HPR-L. BALB/c nude mice were subcutaneously inoculated with A375 melanoma cells in the right axillary fossae to establish malignant melanoma-bearing nude mouse models. Ten nude mouse models were randomly and equally divided into 2 groups to be injected with near-infrared fluorescent cell membrane label (DiR) solution or DiR liposomes (DiR-L) at the same concentration in the caudal vein, and a live imaging system was used to observe the distribution of DiR or DiR-L in nude mice at 6, 12, 24 hours after the injection. Another 30 nude mice were randomly and equally divided into 3 groups to be injected with 5% (mass fraction) glucose solution at a single-dose of 0.2 ml (control group) , 25 mg/kg 4-HPR solution (4-HPR group) and 25 mg/kg 4-HPR-L solution (4-HPR-L group) respectively on days 8, 10, 12, 14, 16, 18, 20 and 22 after the inoculation with A375 cells. The mouse body weight and tumor volume were dynamically monitored in the above groups after the injection, and the survival situation was observed. The nude mice were sacrificed on day 2 after the final injection, and the heart, liver, spleen, lung, kidney and tumor tissues were resected. These tissues were subjected to hematoxylin-eosin staining and immunohistochemical staining to observe the metastasis of melanoma in mice, and terminal deoxyribonucleotidyl transferase-mediated nick end labelling was performed to detect the apoptosis in tumor cells. One-way analysis of variance and independent-sample t test were used to analyze measurement data. Results The live imaging system showed that DiR-L could be retained in melanoma for a long time, and strong fluorescence of DiR-L could be still observed in the tumors at 24 hours after injections. Quantitative fluorescence analysis revealed that the fluorescence intensity of DiR-L (22.85 ± 1.66) was significantly higher than that of DiR in tumor tissues (8.45 ± 0.97, t = 12.957, P < 0.01) . Compared with the control group and 4-HPR group, the resected tumor weight on day 2 after the final injection was significantly decreased in the 4-HPR-L group (F = 27.055, t = 4.768, 6.640, respectively, both P < 0.05) . Hematoxylin-eosin staining showed that liver metastasis occurred in 2 nude mice in the 4-HPR-L group, but in all the nude mice in the control group and 4-HPR group. All the nude mice in the 4-HPR-L group died within 76 days after inoculation, and the mice in the control group and 4-HPR group all died within 56 and 59 days respectively after inoculation. There were significant differences in the apoptotic index among the control group (12.14‰ ± 1.33‰) , 4-HPR group (67.17‰ ± 15.18‰) and 4-HPR-L group (152.73‰ ± 11.27‰; F = 167.588, P < 0.05) , and the apoptotic index was significantly higher in the 4-HPR-L group than in the control group and 4-HPR group (t = 18.162, 11.075 respectively, both P < 0.05) . Conclusion 4-HPR-L can effectively inhibit the growth of subcutaneous melanoma in nude mice and metastasis of melanoma cells, and prolong the survival duration of nude mice. Key words: Nevi and melanomas; Fenretinide; Liposomes; Models, animal; Apoptosis; A375 cells
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- 2020
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24. AXIN2 and SNAIL expression predict the risk of recurrence in cutaneous squamous cell carcinoma after Mohs micrographic surgery
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Guohua Zhao, Zhenlong Zheng, Ki Yeol Kim, Zhehu Jin, Dae San Yoo, Kee Yang Chung, Yeongjoo Oh, Mi Ryung Roh, and Myung Eun Lee
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0301 basic medicine ,Oncology ,predictive nomogram ,Cancer Research ,medicine.medical_specialty ,recurrence ,Snail ,axis inhibition protein 2 ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,biology.animal ,medicine ,Oncogene ,biology ,SNAIL ,business.industry ,Cancer ,Articles ,cutaneous squamous cell carcinoma patients ,mohs micrographic surgery ,Cell cycle ,Nomogram ,medicine.disease ,Molecular medicine ,030104 developmental biology ,030220 oncology & carcinogenesis ,Immunohistochemistry ,business ,Complication - Abstract
Recurrence is a common complication observed during cutaneous squamous cell carcinoma (cSCC) treatment; however, biomarkers for predicting recurrence in cSCC remain unknown. The present study aimed to investigate the predictive value of axis inhibition protein 2 (AXIN2) and SNAIL expression in cSCC recurrence. AXIN2 and SNAIL expression was evaluated using immunohistochemistry in 111 cSCC tissue samples obtained from 18 patients who presented recurrence (recurrence interval, 1–91 months) and 93 patients who did not experience recurrence following Mohs micrographic surgery (MMS) during the follow-up period (156 months). Nomogram construction was performed using patients' clinicopathological characteristics and AXIN2 and SNAIL protein expression. The results demonstrated that high AXIN2 (histoscore >100) and SNAIL (histoscore >100) expression was detected in 35 and 44 cSCC tissues, respectively. Furthermore, the expression levels of AXIN2 and SNAIL were significantly associated in patients with cSCC (P=0.001). AXIN2 and SNAIL expression levels were significantly associated with tumor size (P=0.021 and P=0.044, respectively) and recurrence of cSCC (P=0.017 and P=0.042, respectively). In addition, the results of the Kaplan-Meier curve analysis revealed that recurrence-free survival was significantly associated with tumor size (P=0.025), differentiation status (P
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- 2020
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25. Comparison of Chondrogenic Ability between Mesenchymal Stem Cells and Buffy Coat in Vitro
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Zhongye Zhang, Kumar Gurung, Longhao Jin, Yongjun Jin, Yanqun Liu, Zhehu Jin, Yuanming He, and Yu Jin
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medicine.anatomical_structure ,Cartilage ,Growth factor ,medicine.medical_treatment ,Mesenchymal stem cell ,medicine ,Buffy coat ,Bone marrow ,Subculture (biology) ,Biology ,Chondrogenesis ,Molecular biology ,In vitro - Abstract
Objective: In vitro comparison of chondrogenic differentiation ability of bone marrow extracted mesenchymal stem cells (MSCs) and buffy coat. Methods: MSCs of New Zealand white rabbits were cultured in vitro and adherent cells were passaged. The cells were inoculated on polyglycolic acid (PGA) scaffold (3 mm in diameter and 2 mm in height) liquid for 21 days. Under the same conditions, without subculture, buffy coat was directly inoculated into the cell scaffold. The degree of chondrogenic differentiation was compared by Safranin-Ostaining, histological scoring and biochemical functional detection. Results: The chondrogenic differentiation ability of the buffy coat group was better than that of the MSC group. Safranin-Ostain was stronger in buffy coat group than in MSC group. The Bern Score was also higher in the buffy coat group than in the MSC group and the total amount of sulfated glycosaminoglycans (GAGs) in buffy coat group was higher than that in MSC group. Conclusion: The chondrogenic differentiation ability of buffy coat is higher than that of MSC. Through this result, it was found buffy coat can also differentiate into sub-cartilage without stimulating growth factor. Thus, buffy coat can make a great source in artificial cartilage engineering.
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- 2018
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26. A New Secoiridoid from the Stem Bark of Syringa reticulata
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Zhehu Jin, Gao Li, Peng Pan, Mei Jin, and Wei Zhou
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Stem bark ,biology ,Traditional medicine ,010405 organic chemistry ,Chemistry ,Syringa reticulata ,Plant Science ,General Chemistry ,biology.organism_classification ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,0104 chemical sciences ,Syringin ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Oleuropein ,Oleaceae ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
A new secoiridoid, (1S,5R,8E)-1-butyl-ligstroside aglycon (1), and five known compounds, including ligstroside (2), oleuropein (3), jasminoid D (4), methyloleoside 7-ethyl ester (5), and syringin (6), were isolated from the stem bark of Syringa reticulata (Blume) H. Hara (Oleaceae). Their structures were elucidated using HR-ESI-MS, 1D and 2D NMR spectroscopy, and comparison with the literature.
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- 2019
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27. Syndecan-1 regulates extracellular matrix expression in keloid fibroblasts via TGF-β1/Smad and MAPK signaling pathways
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Chenglong Jin, Shan Jin, Jing Cui, and Zhehu Jin
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0301 basic medicine ,MAPK/ERK pathway ,animal structures ,MAP Kinase Signaling System ,Smad Proteins ,SMAD ,030226 pharmacology & pharmacy ,General Biochemistry, Genetics and Molecular Biology ,Syndecan 1 ,Extracellular matrix ,Transforming Growth Factor beta1 ,03 medical and health sciences ,0302 clinical medicine ,Keloid ,Western blot ,medicine ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,skin and connective tissue diseases ,medicine.diagnostic_test ,Chemistry ,General Medicine ,Fibroblasts ,medicine.disease ,Cell biology ,Extracellular Matrix ,carbohydrates (lipids) ,030104 developmental biology ,embryonic structures ,Syndecan-1 ,Signal transduction ,Transforming growth factor - Abstract
Aims The present study aimed to explore the effect of syndecan-1 on keloid fibroblasts. Main methods Immunohistochemistry and Western blot were employed to assess the expression of syndecan-1. Primary cultured keloid fibroblasts were transfected with syndecan-1 siRNA. The function of syndecan-1 on the proliferation of keloid fibroblasts was investigated through Cell Counting Kit-8 (CCK-8) and flow cytometry. Extracellular matrix, TGF-β1/Smad, and MAPK related proteins were evaluated by Western blot. Key findings Syndecan-1 was significantly overexpressed in both keloid tissues and fibroblasts. Moreover, the knockdown of syndecan-1 remarkably attenuated the proliferation of keloid fibroblasts and reduced the content of the extracellular matrix. Importantly, syndecan-1 regulates the expression of the extracellular matrix in keloid fibroblasts via TGF-β1/Smad and mitogen-activated protein kinase (MAPK) signaling pathways. Significance The current results revealed a crucial function for syndecan-1 in regulating the expression of extracellular matrix and cell proliferation, thereby designating syndecan-1 as a novel target for keloid.
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- 2019
28. A novel technique for treating atrophic facial scars in Asians using ultra-pulse CO
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Wenyan, Jin, Zhouna, Li, Zhehu, Jin, and Chenglong, Jin
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Adult ,Male ,Cosmetic Techniques ,Middle Aged ,Severity of Illness Index ,Cicatrix ,Treatment Outcome ,Asian People ,Erythema ,Face ,Acne Vulgaris ,Lasers, Gas ,Humans ,Female ,Atrophy ,Follow-Up Studies ,Skin - Abstract
Fractional lasers have become increasingly popular for treating atrophic scars, but their effectiveness is limited for deeper scars. We developed a novel technique (manual fractional thermal contraction technology, MFTCT) using an ultra-pulse COA total of 44 patients with atrophic facial scars were treated with MFTCT every 8 weeks for 1-4 times. Overall scar improvement was assessed by photographs taken at baseline and 3 months after the last treatment according to the 4-point global assessment scale (GAS) and ECCA grading scale. Improvements in color, distortion, and texture were assessed by the modified Manchester Scar Scale and scored individually from 1 to 4. Pain degrees and adverse reactions during and after treatment were recorded.A total of 44 patients completed the treatment and follow-ups; of them, 89% reported at least 50% overall improvement after the last treatment. The mean ECCA scores fell from 67.50 ± 23.98 to 45.68 ± 18.57 (a 32% improvement), and the change was significant (P = .000). The average score for overall improvement was 3.48. The average scores for color, distortion, and texture were 3.07 ± 0.62, 3.27 ± 0.50, and 3.52 ± 0.51, respectively. Mean pain degree score was 4.27 ± 1.04, and mean erythema duration was 28.43 ± 6.58 days. Some patients developed pigmentation for a few months that resolved with topical treatment.Manual fractional thermal contraction technology has definite clinical efficacy in the treatment of atrophic facial scars with fewer adverse reactions and is worth using in the clinical setting.
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- 2019
29. Overexpression and Implications of Melanoma-associated Antigen A12 in Pathogenesis of Human Cutaneous Squamous Cell Carcinoma
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Hae Seok Park, Mi Ryung Roh, Zhenlong Zheng, Kee Yang Chung, Guohua Zhao, Zhehu Jin, and Jung Yoon Bae
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Adult ,Cyclin-Dependent Kinase Inhibitor p21 ,Male ,Cancer Research ,Skin Neoplasms ,Repressor ,Mice, Nude ,Biology ,Pathogenesis ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Antigen ,In vivo ,Antigens, Neoplasm ,Cell Movement ,Cell Line, Tumor ,Biomarkers, Tumor ,Animals ,Humans ,Neoplasm Invasiveness ,Aged ,Cell Proliferation ,Aged, 80 and over ,Gene knockdown ,Melanoma-associated antigen ,Mice, Inbred BALB C ,General Medicine ,Middle Aged ,In vitro ,Progression-Free Survival ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Carcinoma, Squamous Cell ,Immunohistochemistry ,Female ,Signal Transduction - Abstract
Background/aim Melanoma-associated antigen A12 (MAGEA12) has recently been reported as a repressor of tumor-suppressor genes. This study aimed to investigate the implications of MAGEA12 expression in the pathogenesis of cutaneous squamous cell carcinoma (cSCC). Materials and methods MAGEA12 and p21 expression were investigated in 15 samples of normal skin and 111 of cSCC tissues by immunohistochemistry. The biological functions of MAGEA12 in cSCC were also investigated both in vitro and in vivo. Results Expression of both MAGEA12 and p21 was significantly increased in cSCC. MAGEA12 expression showed a positive correlation, while p21 expression showed negative correlation with the recurrence-free survival of patients with cSCC. In addition, MAGEA12 knockdown significantly attenuated proliferative, migratory, invasive, and tumorigenic activities of cSCC cells and was negatively correlated with p21 expression both in vitro and in vivo. Conclusion MAGEA12-mediated down-regulation of p21 may be involved in cSCC pathogenesis and MAGEA12 may serve as a molecular biomarker in cSCC.
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- 2019
30. Lipid nano-bubble combined with ultrasound for anti-keloids therapy
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Qiming Wang, Xiao Qing Wang, Zhonggao Gao, Hong-Yan Jin, Zhehu Jin, and Zhou-Na Li
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0301 basic medicine ,Pathology ,medicine.medical_specialty ,Materials science ,Fenretinide ,Cell Survival ,Surface Properties ,Mice, Nude ,Pharmaceutical Science ,Apoptosis ,03 medical and health sciences ,0302 clinical medicine ,Keloid ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Cytotoxic T cell ,Particle Size ,skin and connective tissue diseases ,Cell Proliferation ,Mice, Inbred BALB C ,Liposome ,business.industry ,Ultrasound ,Cancer ,Fibroblasts ,medicine.disease ,Lipids ,In vitro ,030104 developmental biology ,Ultrasonic Waves ,030220 oncology & carcinogenesis ,Liposomes ,Microbubbles ,Nanoparticles ,business - Abstract
Keloids were characterized by excessive growth of fibrous tissues, and shared several pathological characteristics with cancer. They did put physical and emotional stress on patients in that keloids could badly change appearance of patients. N-(4-hydroxyphenyl) retinamide (4HPR) showed cytotoxic activity on a wide variety of invasive-growth cells. Our work was aim to prepare N-(4-hydroxyphenyl) retinamide-loaded lipid microbubbles (4HPR-LM) combined with ultrasound for anti-keloid therapy. 4HPR-loaded liposomes (4HPR-L) were first prepared by film evaporation method, and then 4HPR-LM were manufactured by mixing 4HPR-L and perfluoropentane (PFP) with ultrasonic cavitation method. The mean particle size and entrapment efficiency 4HPR-LM were 113 nm and 95%, respectively. The anti-keloids activity of 4HPR-LM was assessed with BALB/c nude mice bearing subcutaneous xenograft keloids model. 4HPR-LM, combined with ultrasound, could significantly induce apoptosis of keloid fibroblasts in vitro and inhibited growth of keloids in vivo. Thus, 4HPR-LM could be considered as a promising agent for anti-keloids therapy.
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- 2016
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31. Comparative effect and safety of verapamil in keloid and hypertrophic scar treatment: a meta-analysis
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Zhouna Li and Zhehu Jin
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medicine.medical_specialty ,Pathology ,verapamil ,Therapeutics and Clinical Risk Management ,030230 surgery ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Hypertrophic scar ,hypertrophic scars ,0302 clinical medicine ,Keloid ,medicine ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,skin and connective tissue diseases ,Fibroblast ,Pathological ,keloids ,Original Research ,Chemical Health and Safety ,business.industry ,General Medicine ,medicine.disease ,Dermatology ,medicine.anatomical_structure ,Meta-analysis ,Verapamil ,IPL ,Hypertrophic scars ,business ,Wound healing ,Safety Research ,medicine.drug ,steroids - Abstract
Zhouna Li, Zhehu Jin Department of Dermatology, Yanbian University Affiliated hospital, Yanji, Jilin, People’s Republic of China Background: Keloids and hypertrophic scars are the most common types of pathological scarring. Traditionally, keloids have been considered as a result of aberrant wound healing, involving excessive fibroblast participation that is characterized by hyalinized collagen bundles. However, the usefulness of this characterization has been questioned. In recent years, studies have reported the appropriate use of verapamil for keloids and hypertrophic scars.Methods: Searches were conducted on the databases Medline, Embase, Cochrane, PubMed, and China National Knowledge Infrastructure from 2006 to July 2016. State12.0 was used for literature review, data extraction, and meta-analysis. Treatment groups were divided into verapamil and nonverapamil group. Nonverapamil group includes steroids and intense pulsed light (IPL) therapy. Total effective rates include cure rate and effective rate. Cure: skin lesions were completely flattened, became soft and symptoms disappeared. Efficacy: skin lesions subsided, patient significantly reduced symptoms. Inefficient definition of skin was progression free or became worse. Random-effects model was used for the meta-analysis.Results: Six studies that included 331 patients with keloids and hypertrophic scars were analyzed. Analysis of the total effective rate of skin healing was performed. The total effective rates in the two groups were 54.07% (verapamil) and 53.18% (nonverapamil), respectively. The meta-analysis showed that there was no difference between the two groups. We also compared the adverse reactions between the verapamil treatment group and the steroids treatment group in two studies, and the result indicated that the verapamil group showed less adverse reactions.Conclusion: There were no differences between the application of verapamil and nonverapamil group in keloids and hypertrophic scars treatment. Verapamil could act as an effective alternative modality in the prevention and treatment of keloid and hypertrophic scars. A larger number of studies are required to confirm our conclusion. Keywords: keloids, hypertrophic scars, verapamil, steroids, IPL
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- 2016
32. Docetaxel-loaded PEG-albumin nanoparticles with improved antitumor efficiency against non-small cell lung cancer
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Guangming Jin, Xue-Zhe Yin, Mingji Jin, Zhehu Jin, and Zhonggao Gao
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Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,Cell ,Mice, Nude ,Antineoplastic Agents ,Docetaxel ,macromolecular substances ,02 engineering and technology ,Polyethylene Glycols ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Albumins ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Internal medicine ,medicine ,Animals ,Humans ,Lung cancer ,Cytotoxicity ,neoplasms ,Drug Carriers ,Mice, Inbred BALB C ,Oncogene ,business.industry ,technology, industry, and agriculture ,Cancer ,General Medicine ,Cell cycle ,021001 nanoscience & nanotechnology ,medicine.disease ,Xenograft Model Antitumor Assays ,Molecular medicine ,medicine.anatomical_structure ,A549 Cells ,030220 oncology & carcinogenesis ,Cancer research ,Nanoparticles ,Female ,Taxoids ,0210 nano-technology ,business ,medicine.drug - Abstract
The aim of the present study was mainly to assess the advantage of docetaxel-loaded PEG-albumin nanoparticles (PEG-DANPs) against non-small cell lung cancer (NSCLC) compared with the commercial product of docetaxel (Aisu®) and docetaxel-albumin nanoparticles (DANPs). We made systematic assessments on these three drugs against NSCLC both in vitro and in vivo. Based on our experiments, PEG-DANPs showed a dose- and time-dependent efficacy in the in vitro cytotoxicity studies; the tumors growth and the metastases in the livers of NSCLC-bearing nude mice in vivo were reduced dmarkedly by PEG-DANPs, and the PEG-DANP-treated mice had a minimum of weight loss; furthermore, the mice which were treated with PEG-DANPs can survive longer than the other groups. In conclusion, the PEG-DANPs have the lowest side-effects, and the highest antitumor and metastases activity of the three drugs, and it may provide an alternative to patients with NSCLC.
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- 2016
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33. Topical Application of JAK1/JAK2 Inhibitor Momelotinib Exhibits Significant Anti-Inflammatory Responses in DNCB-Induced Atopic Dermatitis Model Mice
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Qiming Wang, Wenyu Jin, Zhehu Jin, Liqing Chen, Mingji Jin, Zhonggao Gao, and Wei Huang
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Male ,0301 basic medicine ,Administration, Topical ,medicine.medical_treatment ,Anti-Inflammatory Agents ,lcsh:Chemistry ,0302 clinical medicine ,Dinitrochlorobenzene ,cytokine ,Medicine ,Phosphorylation ,STAT3 ,lcsh:QH301-705.5 ,Spectroscopy ,Skin ,Mice, Inbred BALB C ,biology ,atopic dermatitis ,General Medicine ,Atopic dermatitis ,Mast cell ,Computer Science Applications ,STAT Transcription Factors ,medicine.anatomical_structure ,Cytokine ,030220 oncology & carcinogenesis ,Benzamides ,Cytokines ,Female ,medicine.symptom ,DNCB ,dendritic cell ,OX40 Ligand ,Inflammation ,Article ,Catalysis ,Dermatitis, Atopic ,Inorganic Chemistry ,03 medical and health sciences ,Thymic Stromal Lymphopoietin ,JAK/STAT pathway ,Animals ,RNA, Messenger ,Physical and Theoretical Chemistry ,Protein Kinase Inhibitors ,Molecular Biology ,CD86 ,business.industry ,Organic Chemistry ,Dendritic Cells ,Janus Kinase 1 ,Dendritic cell ,Immunoglobulin E ,Janus Kinase 2 ,momelotinib ,medicine.disease ,Mice, Inbred C57BL ,Disease Models, Animal ,Pyrimidines ,030104 developmental biology ,Gene Expression Regulation ,lcsh:Biology (General) ,lcsh:QD1-999 ,Immunology ,biology.protein ,business ,CD80 - Abstract
Atopic dermatitis (AD) is a chronic recurrent skin disease dominated by T-helper 2 inflammation. Momelotinib (MMB) is a novel JAK1/JAK2 inhibitor suppressing the signal transduction of multiple pro-inflammatory cytokines. Recent studies indicated that JAK inhibitor could play a therapeutic role in AD disease. In this study, we evaluated the efficacy of MMB as a novel JAK1/JAK2 inhibitor in DNCB-induced AD mice and TSLP-activated dendritic cells. Our data showed that topical application of MMB reduced the skin severity scores and total serum IgE levels, and alleviated the histological indexes including epidermal thickness measurement and mast cell number. Also, it was demonstrated that MMB down-regulated the mRNA expression of IL-4, IL-5, IFN-&gamma, and TSLP, and inhibited the phosphorylation of STAT1, STAT3 and STAT5 in skin lesions. Moreover, MMB reduced the expression of CD80, CD86, MHCII and mRNA of OX40L in TSLP-activated dendritic cells. In general, our study suggests that MMB can improve the symptoms of AD and topical application of MMB can become a promising new therapy strategy for AD.
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- 2018
34. Anti-inflammatory activities of the chemical constituents isolated from
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Mei, Jin, Wei, Zhou, Chunshi, Jin, Zhe, Jiang, Shengbao, Diao, Zhehu, Jin, and Gao, Li
- Subjects
Trametes ,Sterols ,Phenols ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Anti-Inflammatory Agents ,Agaricales ,Nitric Oxide ,Triterpenes - Abstract
Twenty-seven compounds including nine triterpenoids (
- Published
- 2018
35. Anti-inflammatory activities of the chemical constituents isolated from Trametes versicolor
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Zhe Jiang, Zhehu Jin, Gao Li, Chunshi Jin, Shengbao Diao, Wei Zhou, and Mei Jin
- Subjects
chemistry.chemical_classification ,biology ,Traditional medicine ,010405 organic chemistry ,medicine.drug_class ,Organic Chemistry ,Glycoside ,Plant Science ,biology.organism_classification ,01 natural sciences ,Biochemistry ,Anti-inflammatory ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Triterpenoid ,chemistry ,Furan ,Chemical constituents ,medicine ,Phenols ,Trametes versicolor ,Polyporaceae - Abstract
Twenty-seven compounds including nine triterpenoids (1–9), eight sterols (10–17), two ribonucleotides (18, 19), four phenols (20–23), three glycosides (24–26), and one furan (27) were isolated from the fruiting bodies of Trametes versicolor (L.) Lloyd. This study is the first confirmation of the presence of the 11 compounds (3, 5, 6, 8, 18, 20, 21, 23–25, and 27) isolated from the Polyporaceae family, with six of these (2 and 12–16) from the genus Trametes. Compounds 3, 4, 10, 11, 16 and 17 were found to significantly inhibit the production of NO, TNF-α and IL-6 in a dose-dependent manner.
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- 2018
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36. A comparative study on the effect of docetaxel-albumin nanoparticles and docetaxel-loaded PEG-albumin nanoparticles against non-small cell lung cancer
- Author
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Zhonggao Gao, Mingji Jin, Guangming Jin, Zhehu Jin, Liqing Chen, and Xue-Zhe Yin
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,Docetaxel ,Pharmacology ,Polyethylene Glycols ,Mice ,In vivo ,Albumins ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,PEG ratio ,medicine ,Zeta potential ,Animals ,Humans ,Cytotoxicity ,Cell Proliferation ,Drug Carriers ,business.industry ,medicine.disease ,Xenograft Model Antitumor Assays ,Hemolysis ,In vitro ,Oncology ,Nanoparticles ,Taxoids ,business ,Drug carrier ,medicine.drug - Abstract
The present study mainly compared the effect of docetaxel-albumin nanoparticles (DANPs) and docetaxel-loaded PEG-albumin nanoparticles (PEG-DANPs) against non-small cell lung cancer (NSCLC). We made systematic assessments on these three drugs against NSCLC both in vitro and in vivo. With the purpose of eliminating side-effects of the commercial formulation (Tween-80) and prolonging the blood circulation time, we used emulsion-evaporation cross-link method to prepare DANPs and PEG-DANPs. The DANPs had an average particle size of 163.4 ± 3.76 nm, a zeta potential of -19.4 ± 0.18 mV, a polydispersity index of 0.143 ± 0.03, a drug loading of 8.71 ± 0.98%, and an encapsulation efficiency of 93.58 ± 0.86%; the average particle size of PEG-DANPs is 169.19 ± 2.36 nm, zeta potential is -18.2 ± 0.21 mV, with a polydispersity index of 1.56 ± 0.05, a drug loading of 8.72 ± 1.05% and an encapsulation efficiency of 95.4 ± 5.5%. PEG-DANPs showed a dose- and time-dependent efficacy in cytotoxicity studies in vitro; the hemolysis test indicated that PEG-DANPs had less hemocytolysis than Aisu® and DANPs; in addition, a more prolonged circulation time and sustained in vitro release behavior were observed in the PEG-DANPs compared with Aisu® and DANPs; the cellular uptake test in vitro demonstrated that PEG-DANPs could be absorbed easier into the nucleus; furthermore, the tumor growth of NSCLC-bearing nude mice in vivo was reduced the most by PEG-DANPs. In conclusion, the PEG-DANPs have the lowest side-effects, the highest antitumor activity with the longest blood circulation time of the three drugs, and it will provide an alternative to patients with NSCLC.
- Published
- 2015
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37. Decreased expression of protein tyrosine phosphatase non-receptor type 12 is involved in the proliferation and recurrence of bladder transitional cell carcinoma
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Xiankui Liu, Kui-Chang Yuan, Zhehu Jin, Xuan-Shun Jin, Wenyuan Wu, Zhenhua Lin, and Yong-Rui Piao
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Cancer Research ,Pathology ,medicine.medical_specialty ,Oncogene ,Cell ,Articles ,Protein tyrosine phosphatase ,Cell cycle ,Biology ,urologic and male genital diseases ,medicine.disease ,Molecular medicine ,female genital diseases and pregnancy complications ,medicine.anatomical_structure ,Transitional cell carcinoma ,Oncology ,medicine ,Cancer research ,Immunohistochemistry ,Urothelium ,neoplasms - Abstract
Protein tyrosine phosphatase non-receptor type 12 (PTPN12) has been shown to be involved in the development of a number of types of carcinoma. However, the effect of PTPN12 on the proliferation and recurrence of human bladder transitional cell carcinoma (TCC) is unclear. The present study aimed to investigate the expression and function of PTPN12 in human TCC. Samples from 164 patients with TCC, in addition to 146 patients undergoing bladder surgery for indications other than TCC, were examined. PTPN12 protein expression was examined using immunohistochemistry and western blotting, and PTPN12 mRNA expression was examined using reverse transcription-quantitative polymerase chain reaction. PTPN12 expression was increased following transfection with the PTPN12-expressing, pcDEF3 vector, and PTPN12 expression was decreased by RNA interference, in four TCC cell lines. The proliferation of TCC cells was analyzed by a WST-1 assay and in xenografts on BALB/C nude mice. The effect of PTPN12 on tumor recurrence was analyzed by adhesion, migration and invasion assays in TCC cell lines. PTPN12 expression was significantly decreased in TCC tissues compared with that in normal urothelium, and the level of PTPN12 expression was negatively correlated with tumor size, pathological grade, clinical stage and tumor recurrence. Furthermore, decreased expression of PTPN12 significantly enhanced the proliferation of TCC cells in vitro and in vivo. TCC cells with lower levels of PTPN12 exhibited greater adhesion, migration and invasion. In conclusion, PTPN12 expression is downregulated in human TCC. Restoring PTPN12 activity may represent a novel therapeutic strategy for this disease.
- Published
- 2015
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38. Analysis of Tim-3 as a therapeutic target in prostate cancer
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Yong-Rui Piao, Xuan-Shun Jin, Zhehu Jin, and Kui-Chang Yuan
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,T cell ,General Medicine ,Biology ,Hyperplasia ,urologic and male genital diseases ,medicine.disease ,Metastasis ,Flow cytometry ,Prostate cancer ,medicine.anatomical_structure ,Prostate ,medicine ,Cancer research ,Cytotoxic T cell ,CD8 - Abstract
T cell immunoglobulin domain and mucin domain-containing molecule 3 (Tim-3) is a newly discovered immunomodulatory, which plays an important role in immunity regulation. Recent evidence suggests that Tim-3 is differentially regulated in a variety of tumors and has a potential as a therapeutic target. The aim of this study was to investigate the effect of Tim-3 on the development of prostate cancer (PCa). Tim-3 expressing on peripheral CD4+ T and CD8+ T cells was analyzed by flow cytometry. The relationships between Tim-3 expression and clinicopathological features were analyzed. Immunohistochemical expression of Tim-3 was examined in our large numbers of paraffin-fixed prostate tissues. Flow cytometry revealed that expression of Tim-3 was significantly increased on both CD4+ and CD8+ T cells in PCa patients than that in benign prostate hyperplasia (BPH) patients. Also, the level of Tim-3 on CD4+ T cells was positively correlated with CD8+ T cells in patients. Further analyses revealed that the levels of Tim-3 on CD4+ T cells and CD8+ T cells exhibited different expression patterns in terms of localization depending on pathological category of PCa and metastasis. Immunohistochemical analysis revealed that positive staining of Tim-3 in PCa but little or no staining of Tim-3 was observed in BPH epithelium. Tim-3 may affect the development and progression of PCa, which may provide knowledge for using Tim-3 as a novel therapy for effective PCa management.
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- 2014
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39. RUNX3 expression is associated with sensitivity to pheophorbide a-based photodynamic therapy in keloids
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Sook Moon, Mi Ryung Roh, Zhenlong Zheng, Lianhua Li, Lianhua Zhu, Xianglan Zhang, and Zhehu Jin
- Subjects
Adult ,Chlorophyll ,Male ,Pathology ,medicine.medical_specialty ,Adolescent ,Cell Survival ,medicine.medical_treatment ,Down-Regulation ,Apoptosis ,Photodynamic therapy ,Dermatology ,Collagen Type I ,Young Adult ,Keloid ,Dermis ,Proliferating Cell Nuclear Antigen ,medicine ,Humans ,Viability assay ,Child ,skin and connective tissue diseases ,Aged ,Cell Proliferation ,Skin ,Aged, 80 and over ,biology ,business.industry ,Fibroblasts ,Middle Aged ,medicine.disease ,digestive system diseases ,Proliferating cell nuclear antigen ,Core Binding Factor Alpha 3 Subunit ,medicine.anatomical_structure ,Photochemotherapy ,Cancer cell ,biology.protein ,Immunohistochemistry ,Female ,Surgery ,Reactive Oxygen Species ,business ,Type I collagen - Abstract
Runt-related transcription factor 3 (RUNX3) has recently been reported to be a possible predictor of sensitivity of cancer cells for photodynamic therapy (PDT), a promising therapeutic modality for keloids. In this study, we aimed to elucidate the implications of RUNX3 for keloid pathogenesis and sensitivity to pheophorbide a-based PDT (Pa-PDT). RUNX3 and proliferating cell nuclear antigen (PCNA) expression were examined in 6 normal skin samples and 32 keloid tissue samples by immunohistochemistry. We found that RUNX3 expression was detected more often in keloid tissues than in dermis of normal skin. In keloid tissues, RUNX3 expression was significantly increased in patients presenting with symptoms of pain or pruritus, and was also significantly related to PCNA expression. The therapeutic effect of Pa-PDT was comparatively investigated in keloid fibroblasts (KFs) with and without RUNX3 expression. Significant differences were found after Pa-PDT between KFs with and without RUNX3 expression in cell viability, proliferative ability, type I collagen expression, generation of reactive oxygen species (ROS), and apoptotic cell death. In addition, RUNX3 expression was significantly decreased after Pa-PDT in KFs, and KFs with downregulation of RUNX3 showed significantly increased cell viability after Pa-PDT. Pa-PDT may be a potential therapeutic modality for keloids, and RUNX3, as a possible contributor to keloid pathogenesis, may improve sensitivity to Pa-PDT in KFs.
- Published
- 2014
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40. Prognostic Value of T Cell Immunoglobulin Mucin-3 in Prostate Cancer
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Zhehu Jin, Long-Zhen Piao, Xiu-Zhe Dong, Lian-Hua Zhu, and Yong-Rui Piao
- Subjects
Adult ,Male ,Oncology ,PCA3 ,Cancer Research ,medicine.medical_specialty ,Small interfering RNA ,Lung Neoplasms ,Epidemiology ,T cell ,Blotting, Western ,Apoptosis ,Bone Neoplasms ,Real-Time Polymerase Chain Reaction ,Immunoenzyme Techniques ,Prostate cancer ,Internal medicine ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Humans ,RNA, Messenger ,Hepatitis A Virus Cellular Receptor 2 ,Aged ,Cell Proliferation ,Neoplasm Staging ,Aged, 80 and over ,Gene knockdown ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,Cell growth ,Public Health, Environmental and Occupational Health ,Membrane Proteins ,Prostatic Neoplasms ,Middle Aged ,Prognosis ,medicine.disease ,Survival Rate ,medicine.anatomical_structure ,Lymphatic Metastasis ,biology.protein ,Immunohistochemistry ,Neoplasm Grading ,Neoplasm Recurrence, Local ,Antibody ,business ,Follow-Up Studies - Abstract
Background: Optimal treatment for prostate cancer remains a challenge worldwide. Recently, T cell immunoglobulin mucin-3 (TIM-3) has been implicated in tumor biology but its contribution prostate cancer remains unclear. The aim of this study was to investigate the role of TIM-3 as a prognostic marker in patients with prostate cancer. Methods: TIM-3 protein expression was determined by immunohistochemistry and Western blotting in 137 prostate cancer tumor samples and paired adjacent benign tissue. We also performed cell proliferation assays using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl- 2H tetrazolium bromide (MTT) and cell invasion assays. The effects of small interfering RNA (siRNA)-mediated knockdown of TIM-3 (TIM-3 siRNA) in two human prostate cancer cell lines were also evaluated. Results: TIM-3 expression was higher in prostate cancer tissue than in the adjacent benign tissue (P
- Published
- 2013
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41. Hypermethylation and downregulation of glutathione peroxidase 3 are related to pathogenesis of melanoma
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Haiyue Chen, Ki Yeol Kim, Mi Ryung Roh, Zhenlong Zheng, Xuemei Jin, and Zhehu Jin
- Subjects
0301 basic medicine ,Adult ,Male ,Cancer Research ,Tumor suppressor gene ,GPX3 ,Adolescent ,Biology ,Decitabine ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,medicine ,Humans ,RNA, Messenger ,Child ,Promoter Regions, Genetic ,neoplasms ,Melanoma ,Aged ,chemistry.chemical_classification ,Regulation of gene expression ,Aged, 80 and over ,Glutathione Peroxidase ,Glutathione peroxidase ,General Medicine ,Cell cycle ,DNA Methylation ,Middle Aged ,medicine.disease ,Molecular biology ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Oncology ,chemistry ,030220 oncology & carcinogenesis ,DNA methylation ,Cancer research ,Azacitidine ,Female - Abstract
As a crucial antioxidant enzyme, glutathione peroxidase 3 (GPX3) has been found to be frequently repressed in many cancers due to promoter hypermethylation and is known as a possible tumor suppressor gene. In the present study, we investigated whether promoter hypermethylation of GPX3 and its repression are present in melanoma and, if so, whether GPX3 downregulation is implicated in the pathogenesis of melanoma. Our results revealed methylation of GPX3 and downregulation of its expression in both melanoma cell lines and surgical melanoma tissue samples. In melanoma cell lines, GPX3 expression was restored by treatment with 5-aza-2'-deoxycytidine both in mRNA and protein levels. Depletion of GPX3 was found to increase the proliferative ability, motility, and invasiveness of melanoma cells. Moreover, negative expression of GPX3 was related to poor prognosis in melanoma patients. These results suggest that methylation-mediated GPX3 repression may have critical implications for melanoma pathogenesis.
- Published
- 2016
42. Ca2+ channel blocker benidipine promotes coronary angiogenesis and reduces both left-ventricular diastolic stiffness and mortality in hypertensive rats
- Author
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Takao Nishizawa, Xian Wu Cheng, Akiko Noda, Akihiro Hirashiki, Kyosuke Takeshita, Koji Obata, Takeshi Sasaki, Kohzo Nagata, Hideo Izawa, Guo-Ping Shi, Zhehu Jin, Toyoaki Murohara, Masafumi Kuzuya, and Kenji Okumura
- Subjects
Male ,Dihydropyridines ,medicine.medical_specialty ,Physiology ,Heart Ventricles ,Diastole ,Neovascularization, Physiologic ,Article ,Coronary circulation ,chemistry.chemical_compound ,Nitrendipine ,Coronary Circulation ,Internal medicine ,Internal Medicine ,Animals ,Medicine ,Antihypertensive Agents ,Heart Failure ,Rats, Inbred Dahl ,business.industry ,Diastolic heart failure ,Calcium Channel Blockers ,medicine.disease ,Rats ,Endocrinology ,medicine.anatomical_structure ,Blood pressure ,chemistry ,Ventricle ,Heart failure ,Hypertension ,Benidipine ,Cardiology ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
Background The beneficial cardiac effects of some Ca(2+) channel blockers have been attributed to blood pressure reduction, but these pleiotropic effects require further investigation. We compared the effects of benidipine, which has beneficial cardiac effects, and nitrendipine, which does not, in an animal model of hypertensive diastolic heart failure (DHF). Methods and results Male Dahl salt-sensitive rats were fed a high-salt diet from age 7 weeks to induce hypertension and were either vehicle or orally administered benidipine (3 mg/kg daily) or nitrendipine (10 mg/kg daily) from age 10 to 18 weeks. Control rats were maintained on a low-salt diet. In vehicle-treated rats, left-ventricular (LV) fractional shortening was preserved but LV end-diastolic pressure was increased, indicative of DHF. Benidipine and nitrendipine had similar antihypertensive effects and reduced both LV weight and cardiomyocyte hypertrophy. Benidipine reduced LV diastolic stiffness and mortality to a greater extent than did nitrendipine. Benidipine, but not nitrendipine, also reduced lung weight. The extent of interstitial fibrosis and the abundance of mRNAs for prohypertrophic, profibrotic, or proinflammatory genes in the left ventricle were reduced by benidipine and nitrendipine. Benidipine, but not nitrendipine, increased capillary density and restored the expression of hypoxia-inducible factor 1alpha, vascular endothelial growth factor, and endothelial nitric oxide synthase in the left ventricle. Conclusions Benidipine reduced LV diastolic stiffness and increased survival, effects likely attributable predominantly to promotion of coronary angiogenesis rather than to attenuation of interstitial fibrosis. Benidipine may thus be more effective than purely L-type Ca(2+) channel blockers in preventing hypertensive DHF.
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- 2010
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43. Soluble LR11/SorLA represses thermogenesis in adipose tissue and correlates with BMI in humans
- Author
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Joana Relat, Hideaki Bujo, Takeyoshi Murano, Antonio Vidal-Puig, Takashi Yamaguchi, Mark Campbell, Zhehu Jin, Isamu Fukamachi, Ichiro Tatsuno, Andrew J. Whittle, Vivian Peirce, Chiaki Nakaseko, Mao Takahashi, Masahiro Takeuchi, Hiroyuki Ebinuma, Wolfgang J. Schneider, Samuel Virtue, Meizi Jiang, Wenlong Jin, Vidal-Puig, Antonio [0000-0003-4220-9577], and Apollo - University of Cambridge Repository
- Subjects
Male ,medicine.medical_specialty ,General Physics and Astronomy ,Adipose tissue ,Down-Regulation ,White adipose tissue ,Biology ,Bone morphogenetic protein ,Medical sciences ,General Biochemistry, Genetics and Molecular Biology ,Article ,Body Mass Index ,Mice ,Downregulation and upregulation ,Adipose Tissue, Brown ,Internal medicine ,Brown adipose tissue ,medicine ,Genetics ,Animals ,Humans ,Ratolins ,Obesity ,LDL-Receptor Related Proteins ,Ciències de la salut ,Mice, Knockout ,Multidisciplinary ,Membrane Transport Proteins ,Adipose tissues ,Thermogenesis ,General Chemistry ,Metabolisme ,3. Good health ,Mice, Inbred C57BL ,Teixit adipós ,Endocrinology ,medicine.anatomical_structure ,Metabolism ,Receptors, LDL ,Hypermetabolism ,Obesitat ,Female ,Energy Metabolism ,Genètica ,Lipoprotein - Abstract
Thermogenesis in brown adipose tissue (BAT) is an important component of energy expenditure in mammals. Recent studies have confirmed its presence and metabolic role in humans. Defining the physiological regulation of BAT is therefore of great importance for developing strategies to treat metabolic diseases. Here we show that the soluble form of the low-density lipoprotein receptor relative, LR11/SorLA (sLR11), suppresses thermogenesis in adipose tissue in a cell-autonomous manner. Mice lacking LR11 are protected from diet-induced obesity associated with an increased browning of white adipose tissue and hypermetabolism. Treatment of adipocytes with sLR11 inhibits thermogenesis via the bone morphogenetic protein/TGFβ signalling pathway and reduces Smad phosphorylation. In addition, sLR11 levels in humans are shown to positively correlate with body mass index and adiposity. Given the need for tight regulation of a tissue with a high capacity for energy wastage, we propose that LR11 plays an energy conserving role that is exaggerated in states of obesity., Brown adipose tissue (BAT) thermogenesis is an important determinant of organismal energy expenditure in mammals. Here, Whittle et al. report that the protein sLR11 is a negative regulator of BAT activity in mice, repressing thermogenesis by inhibiting BMP/Smad signalling in brown adipocytes.
- Published
- 2015
44. Protein tyrosine phosphatase nonreceptor type 12 suppresses the proliferation of renal cell carcinoma by inhibiting the activity of the PI3K/mTOR pathway
- Author
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Yongrui, Piao, Yong, Rui Piao, and Zhehu, Jin
- Subjects
Adult ,Aged, 80 and over ,Male ,TOR Serine-Threonine Kinases ,Protein Tyrosine Phosphatase, Non-Receptor Type 12 ,Middle Aged ,Prognosis ,Kidney Neoplasms ,Phosphatidylinositol 3-Kinases ,Cell Line, Tumor ,Humans ,Female ,Carcinoma, Renal Cell ,Aged ,Cell Proliferation ,Phosphoinositide-3 Kinase Inhibitors ,Signal Transduction - Abstract
To determine the expression and functions of protein tyrosine phosphatase nonreceptor type 12 (PTPN12) in renal cell carcinoma (RCC).All RCC tissue and corresponding normal kidney tissue from 116 RCC patients undergoing radical nephrectomy were examined. PTPN12 expression was detected by immunohistochemistry, and PTPN12 mRNA expression by real-time polymerase chain reaction (RT-PCR). PTPN12 expression was increased by stable transfection with a pcDEF3 vector containing full-length cDNA of PTPN12 and was decreased by RNAi in 4 RCC cell lines. Proliferative analysis of RCC cells was done using a WST-1 assay and animal xenograft study.PTPN12 expression in RCC tissue was significantly decreased compared with normal kidney tissue, and was overexpressed in larger tumors, metastasis, and high pathological grades.PTPN12 expression decreases in human RCC, it is involved in progression and metastasis, and is an independent prognostic factor in RCC. Restoring PTPN12 activity could be a new therapeutic approach in advanced RCC.
- Published
- 2015
45. Retractions
- Author
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Zhehu Jin and Yong-Rui Piao
- Subjects
Andrology ,Reproductive Medicine ,medicine ,Cell Biology ,General Medicine ,Biology ,medicine.disease ,Sperm ,Function (biology) ,Male infertility - Published
- 2015
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46. Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression
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Mingji Jin, Aili Cui, Lianhua Li, Zhehu Jin, Lianhua Zhu, Zhonggao Gao, Chenglong Jin, and Yinghua An
- Subjects
Male ,0301 basic medicine ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,microRNA ,medicine ,Humans ,Genes, Tumor Suppressor ,Luciferase ,Melanoma ,RC254-282 ,Cell Proliferation ,Gene knockdown ,Cell growth ,Cell Cycle ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,General Medicine ,Cell cycle ,medicine.disease ,Cell biology ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Cell culture ,030220 oncology & carcinogenesis ,Insulin Receptor Substrate Proteins ,Cancer research ,Female ,Signal Transduction - Abstract
Accumulating evidence indicated that aberrantly expressed microRNAs play critical roles in the initiation and progression of human cancers. However, the underlying functions of miR-493 in human melanoma remains unknown. Here, our study found that miR-493 expression was downregulated in human melanoma tissues and cells. Overexpression of miR-493 suppressed cell proliferation and cell cycle in human melanoma cell line A375. IRS4 was defined as a target for downregulation by miR-493 and was confirmed by luciferase assay. We also found that knockdown of IRS4 counteracted the proliferation promotion by miR-493 inhibitor. In summary, these results demonstrated that miR-493 acts as a tumor suppressor and inhibits cell proliferation and cell cycle in human melanoma by directly targeting IRS4.
- Published
- 2017
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47. Forkhead box J1 expression is upregulated and correlated with prognosis in patients with clear cell renal cell carcinoma
- Author
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Xiu-Zhe Dong, Zhehu Jin, Pingyu Zhu, and Yong-Rui Piao
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,Oncogene ,business.industry ,Cell ,Articles ,Cell cycle ,medicine.disease ,medicine.disease_cause ,urologic and male genital diseases ,female genital diseases and pregnancy complications ,Clear cell renal cell carcinoma ,medicine.anatomical_structure ,Oncology ,Renal cell carcinoma ,medicine ,Cancer research ,Immunohistochemistry ,Carcinogenesis ,business ,neoplasms ,Clear cell - Abstract
The forkhead box (FOX) family of transcription factors are considered to have a role in tumorigenesis. FOXJ1 is a member of the FOX family; however, its function in human renal cell carcinoma (RCC) has remained to be elucidated. Therefore, the present study evaluated the expression of FOXJ1 in human clear cell RCC and the effect of FOXJ1 on the proliferative ability of RCC cells. The RCC specimens analyzed in the present study were obtained from 286 patients with RCC who underwent nephrectomy. FOXJ1 mRNA expression levels were determined using reverse transcription-quantitative polymerase chain reaction, and FOXJ1 protein expression levels were determined using immunohistochemistry and western blot analysis. To determine the effect of FOXJ1 on the proliferative ability of RCC cells, the expression of FOXJ1 was decreased using small interfering (si)RNA, and a FOXJ1 vector was stably transfected into RCC cell lines. The proliferative ability of RCC cells was then examined using a WST-1 assay and xenograft experiments with BALB/c nude mice, where the association between FOXJ1 expression and patient survival was determined using Kaplan-Meier analysis. FOXJ1 expression was significantly higher in RCC tissues compared with that of healthy renal tissues. Furthermore, FOXJ1 expression was associated with tumor stage, histologic grade and size. In addition, FOXJ1 significantly enhanced the proliferation of RCC cells in vitro and in vivo. The present study identified that FOXJ1 expression was upregulated in RCC and enhanced the proliferative ability of RCC cells. Therefore, FOXJ1 may serve as an independent prognostic marker and a therapeutic target for the treatment of patients with RCC.
- Published
- 2014
48. Increased c-Met phosphorylation is related to keloid pathogenesis: implications for the biological behaviour of keloid fibroblasts
- Author
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Zhehu Jin
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,C-Met ,Indoles ,Adolescent ,Piperazines ,Pathology and Forensic Medicine ,Pathogenesis ,chemistry.chemical_compound ,Young Adult ,Keloid ,Gene expression ,medicine ,Humans ,Phosphorylation ,skin and connective tissue diseases ,Protein kinase B ,Cells, Cultured ,Aged ,Cell Proliferation ,Skin ,Aged, 80 and over ,Sulfonamides ,Chemistry ,Hepatocyte Growth Factor ,Fibroblasts ,Middle Aged ,Proto-Oncogene Proteins c-met ,medicine.disease ,Ki-67 Antigen ,Cancer research ,Hepatocyte growth factor ,Female ,Collagen ,Signal transduction ,Wound healing ,medicine.drug ,Signal Transduction - Abstract
Keloid is induced by a pathological wound healing response, and hepatocyte growth factor (HGF) is known to be involved in tissue repair via the activation of its primary receptor, c-Met. We aimed to investigate whether c-Met activation is implicated in keloid pathogenesis. HGF, c-Met, phosphorylated c-Met (p-Met), Ki-67, collagen I protein, and MET gene expression were detected in five normal skin and 30 keloid tissues by immunohistochemistry and quantitative real-time polymerase chain reaction analysis, respectively. The influence of p-Met expression on the biological behaviour of keloid fibroblasts was further investigated with regard to cell proliferation, motility, invasiveness, collagen I expression, and intracellular signaling in vitro. p-Met protein and MET gene expression but not HGF or c-Met protein expression showed significant increases in keloid tissues than dermal layer of normal skin tissues. In keloid tissues, p-Met expression was significantly associated with keloid size, Ki-67 and collagen I expression. Moreover, p-Met expression was also related to proliferation, migration, invasiveness, collagen I expression and activation of AKT and Erk in keloid fibroblasts in vitro. c-Met activation may have a strong influence on keloid pathogenesis, and it can be investigated further as a potential molecular target for keloid therapy.
- Published
- 2013
49. Mechanism of Diastolic Stiffening of the Failing Myocardium and Its Prevention by Angiotensin Receptor and Calcium Channel Blockers
- Author
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Kazumasa Unno, Ken Harada, Xian Wu Cheng, Zhehu Jin, Kyosuke Takeshita, Toyoaki Murohara, Masafumi Kuzuya, Koji Obata, Kohzo Nagata, Mitsuhiro Yokota, Akihiro Hirashiki, Aiko Inoue, Kenji Okumura, and Guo-Ping Shi
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Male ,medicine.medical_specialty ,Angiotensin receptor ,Dihydropyridines ,medicine.drug_class ,Azelnidipine ,Diastole ,Tetrazoles ,Calcium channel blocker ,Article ,Receptor, Angiotensin, Type 1 ,Internal medicine ,Renin–angiotensin system ,medicine ,Animals ,Pharmacology ,Heart Failure ,Rats, Inbred Dahl ,Chemistry ,Calcium channel ,Myocardium ,Imidazoles ,medicine.disease ,Calcium Channel Blockers ,Angiotensin II ,Rats ,Endocrinology ,Heart failure ,cardiovascular system ,Cardiology and Cardiovascular Medicine ,Angiotensin II Type 1 Receptor Blockers ,Azetidinecarboxylic Acid ,medicine.drug - Abstract
To investigate the mechanism responsible for the increased cardiac stiffness associated with hypertensive heart failure in Dahl salt-sensitive (DS) rats and the effects of treatment with the combination of a calcium channel blocker [azelnidipine (AZE)] and angiotensin II type 1 receptor blocker [olmesartan (OLM)].DS rats fed a high-salt diet from 7 weeks of age were treated (or not) from 12 to 19 weeks of age with the vasodilator hydralazine, OLM plus AZE, or the reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor apocynin. Rats fed a low-salt diet served as controls.Treatment with OLM plus AZE attenuated changes in the expression of collagen isoforms and a decrease in the ratio of elastin to collagen in the left ventricle and prevented the increase in myocardial stiffness and diastolic dysfunction in DS rats in a manner independent of the hypotensive effect of these drugs. Such treatment also inhibited the expression and activation of elastolytic proteases (including cathepsins S and K and metalloproteinases-2, -9, and -12), NADPH oxidase-dependent superoxide production, and inflammatory changes in the failing myocardium. All these effects were mimicked by treatment with apocynin.The changes in collagen isoform expression and the decrease in the elastin to collagen ratio in the failing myocardium likely account for the increase in diastolic stiffness in this model of hypertensive heart failure. Administration of angiotensin receptor and calcium channel blockers prevented these changes in a manner independent of the hypotensive effect of these drugs by inhibiting the increase in elastolytic activity induced by activation of NADPH oxidase.
- Published
- 2009
50. Comparison of Bypass Surgery with Drug-Eluting Stents in Diabetic Patients with Left Main Coronary Stenosis
- Author
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Guo-Ping Shi, Like Guan, Hui Song, Dongmei Shi, Xian Wu Cheng, Yujie Zhou, Guanbin Zheng, Yonghe Guo, Yuzi Li, Xiaoxiao Zhao, and Zhehu Jin
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Male ,Left main coronary artery disease ,medicine.medical_specialty ,Cardiac & Cardiovascular Systems ,medicine.medical_treatment ,Revascularization ,Diabetes mellitus ,Internal medicine ,drug-eluting stent ,Humans ,Medicine ,cardiovascular diseases ,Angioplasty, Balloon, Coronary ,business.industry ,Hazard ratio ,Coronary Stenosis ,Drug-Eluting Stents ,General Medicine ,Middle Aged ,medicine.disease ,Confidence interval ,Surgery ,Stenosis ,Treatment Outcome ,surgical procedures, operative ,Bypass surgery ,Drug-eluting stent ,diabetes mellitus ,Cohort ,Cardiology ,Female ,Original Article ,coronary intervention ,coronary-artery bypass grafting ,business - Abstract
Purpose Several studies have compared the effects of coronary stenting and coronary-artery bypass grafting (CABG) on left main coronary artery (LMCA) disease. However, there are limited data on the long-term outcomes of these two interventions in diabetic patients. Materials and Methods We evaluated 56 patients with LMCA stenosis who underwent drug-eluting stent (DES) implantation and 116 patients who underwent CABG in a single hospital in China between January 2004 and December 2006. We compared long-term major adverse cardiac events (death; a "serious outcome" composite of death, myocardial infarction, or stroke; and target-vessel revascularization). Results In-hospital (30-day) mortality was 0% for the DES group and 3.4% for the CABG group (p=0.31). There was no difference between the two groups in terms of risk of death [hazard ratio for stenting group, 0.49; 95% confidence interval (CI), 0.13-1.63; p=0.55] or risk of serious outcome (hazard ratio for DES group, 1.11; 95% CI, 0.39-1.45; p=0.47). The target-vessel revascularization rate was higher in the DES group than in the CABG group (hazard ratio, 3.67; 95% CI, 1.24-11.06; p=0.018). Conclusion In this cohort of diabetic patients with LMCA stenosis, there was no difference in composite endpoints between patients receiving DESs and those undergoing CABG. However, stenting was associated with higher rates of target-vessel revascularization than CABG. DES implantation in diabetic patients with LMCA disease was found to be at least as safe as CABG.
- Published
- 2011
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