Naringenin inhibits proliferation, invasion and collagen synthesis in keloid fibroblasts by regulating TGF-β1/Smad signaling pathway

Objective To explore the effects and molecular mechanisms of naringenin (Nar) on the proliferation, migration and collagen synthesis of keloid fibroblasts (KFs). Methods KFs were divided into control group and Nar group treated with Nar at concentrations of 10, 25, 50, 100 and 150 μmol/L, and the effects of Nar on the proliferation of KFs were analyzed using CCK-8 assay. Western blot was performed to detect the effects of Nar on the expression levels of Col-1, FN, α-SMA, and MMP9, and the expression levels of Smad2/3 and p-Smad2/3 in KFs pretreated with TGF-β1. Results Compared with the control group, the proliferation ability of KFs was significantly inhibited by Nar at a concentration of ≥50 μmol/L (P