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Using bis(tridentate)polypyridyl ligands, two new 2 D (two-dimensional) coordination polymers (CPs), {Cu2(tpbn)(2,6-NDC)2}n (1) and {[Cu2(tphn)(2,6-NDC)2]·4H2O}n (2), where tpbn = N,N′,N″,N‴-tetrakis(2-pyridylmethyl)-1,4-diaminobutane, tphn = N,N′,N″,N‴-tetrakis(2-pyridylmethyl)-1,6-diaminohexane, and 2,6-H2NDC = 2,6-naphthalenedicarboxylic acid, were obtained in high yield in a mixed solvent system. 1 and 2 were characterized via single-crystal X-ray diffraction, powder X-ray diffraction, FT-IR spectroscopy and TG analysis. Real-time transcription-polymerase chain reaction (RT-PCR) was conducted to evaluate the relative activity of the miR-155 and miR-34a in the lymph node cells during bacterial infection. After treatment with 1 or 2, the quantity of inflammatory cytokines produced by the lymph node cells was evaluated by enzyme linked immunosorbent assay (ELISA) detection equipment. [ABSTRACT FROM AUTHOR]

The mitochondrial genome was amplified in two large overlapping fragments by long PCR and one gap PCR. The complete Northeastern China burbot mitogenome sequence (KC844053) is 16,575 bp in length. Similar to most vertebrata, the mitogenome has a set of 37 genes: including 13 protein-coding, 22 tRNA and 2 rRNA genes. More than 98% nucleotide sequence was simil to the reported individual. [ABSTRACT FROM AUTHOR]

In this study, we sequenced and determined the complete mitochondrial genome of rosy barb (Puntius conchonius). The circular mitochondrial genome (17,082 bp) consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. This is the first report on the complete mitogenome sequence of rosy barb (P. conchonius). [ABSTRACT FROM PUBLISHER]

We first sequenced the complete mitochondrial genome of silvertip tetra (Hasemania nana). The mitogenome was determined to be 16,581 bp long circular molecule with a typical gene arrangement of vertebrate mitochondrial DNA. All genes were encoded on the heavy strand with the exception ofND6and eight tRNA genes. Mitochondrial DNA information provided the basis for the studies in species identification and conservation of the species’ natural resources. [ABSTRACT FROM PUBLISHER]

During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53 X 10(-4) per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.