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Objective To explore the effect and mechanism of miR-767-5P and transductin β1X linked receptor protein 1 (TBL1XR1) on the progression of ovarian cancer. Methods Database analysis: KEGG (Kyoto Encyclopedia of Genes and Genomes, Kyoto Encyclopedia) enrichment analysis of miR-767-5P using GEO, miRPATH database and RStudio TOP5 microRNAs with differential expression multiples were selected from low and high expression microRNAs in the GEO data set, then ranked from low to high in terms of differential multiples. The target genes for miR-767-5p were predicted by miRDB and other databases. The intersection was selected. The target genes were screened by the Open Target Platform, GEPIA and other databases. GEPIA and THPA databases were used to analyze the expression level of TBL1XR1 in human organs, immunohistochemical analysis and survival curve analysis. Experimental verification: Dual luciferase assay detected the binding target of miR-767-5P and TBL1XR1. Western blot was used to detect TBL1XR1 expression in ovarian cancer cell lines (OC3, SKOV-3, HO-8910) and normal ovarian epithelial cell line(IOSE80). After transferring into SKOV-3 cells with miR-767-5P over-expression plasmid and TBL1XR1 over-expression plasmid, Western blot was used to detect the expression level of TBL1XR1 and Tanswell cell assay was used to detect cell invasion. Results Database analysis: miR-767-5p with the highest differential ratio of low expression was selected as the target microRNA for follow-up research; miR-767-5p was involved in various cancer-related pathways, and miR-767-5p and TBL1XR1 were closely associated with breast cancer and ovarian cancer, etc. TBL1XR1 was highly expressed in ovarian cancer and was associated with poor prognosis. Experimental verification: miR-767-5p and TBL1XR1 could be targetly bound(P＜0.05); Compared with IOSE80, TBL1XR1 was significantly over-expressed in OC3, SKOV-3 and HO-8910 cells. After the transfection of miR-767-5p over-expressed plasmid, the expression level of TBL1XR1 was decreased and the aggressiveness of ovarian cancer cells was reduced(P＜0.05), while simultaneously transfection of TBL1XR1 over-expressed plasmid could reduce this effect to some extent. Conclusions miR-767-5p regulates TBL1XR1 expression and inhibits the invasion of human ovarian cancer cell lines.

Simple Summary: Black locust (Robinia pseudoacacia) and its specialist insects have major effects on ecosystems in Europe, where the former is the only host of the latter. Here, we determined the relative roles of host plant availability and other predictors in shaping the specialist insects' future range dynamics. Range expansions were detected in all target species. Climate predictors were expected to have the strongest effects on the range shifts of the host plant, while host plant availability had the strongest effect on the range shifts of the specialist insects. Therefore, range shifts in specialist insects tracked those of their host. Mitigating future climate change might be one of the key approaches for controlling invasions of Robinia-specialist insects in Europe, given that their range changes follow those of their specialist host plant, and future climate changes were mainly responsible for the range expansions of the host plant. Black locust is the only host of Robinia-specialist insects in Europe. However, no study to date has examined future range shifts of specialist insects, and the relative effects of host plant availability and other factors on their range shifts. Here, we characterized the future range shifts in the host and its four specialist insects and the factors contributing to changes in their ranges. We detected substantial range expansions in all target species. Climate predictors and host plant availability were expected to have the strongest effects on the range shifts in the host and its specialist insects, respectively, suggesting that the specialist insects will track the ranges of their host. Parectopa robiniella showed the largest potential and expanding ranges and should be made a priority species for controlling invasions of Robinia-specialist insects in Europe. The expanding ranges of all specialist species were largely identified in the United Kingdom, Germany, and France, suggesting that these should be priority regions for mitigating their effects on ecosystems. Reducing future climate change is essential for preventing the spreading of specialist insects in Europe since specialist insects track their specialist host plants, and host range expansions are mainly driven by future climate changes. [ABSTRACT FROM AUTHOR]

In the process of civil aircraft production,it is difficult to avoid the situation that the fastener is punched into the bend radius of joint structure,and it is hard to replace the out-of-tolerance parts because of some limits of objective condition. When filler is utilized to fit the bend radius to deal with this issue,the fatigue quality of joint structure will be affected due to the change of stress concentration and the non-standard installation of fastener. In this paper,the influence on the detail fatigue rating(DFR)values of the repaired structure is investigated by fatigue tests. During the design of test specimen,the influence of some parameters on stress concentration factor is analyzed by finite element method. According to the results of finite element analysis,three groups of fatigue tests are carried out to get the corresponding correction coefficient of DFR values. The fatigue test results are compared with the results of engineering calculation method. The results show that,in the case of the out-of-tolerance degree of hi-lock bolt withbend radius is better than that of the hole of HST12AG6 is punched into the bend radius 1.0 mm,the detail fatigue rating values of the joints are decreased within 10%. The failure mode of the fatigue test group which fasteners are installed standardly is completely consistent with the reference group,and the difference of detail fatigue rating values is within the reasonable range.

Simple Summary: Invasive wasps can have huge impacts on global apiculture. Here, we developed 12 range dynamic models to globally calibrate the future range dynamics and invasion hotspots of 12 major invasive Vespidae wasp species through a unified approach and evaluated their future impacts on apiculture worldwide. We detected increases in their habitat suitability in most parts of the globe and substantial range expansions, both mainly driven by future climatic changes. We also identified those invasive wasp species projected to have the largest potential ranges, highest range expansion ratios, and largest centroid shifts, as well as the invasion hotspots of all major invasive wasp species in the future. In summary, the increasing habitat suitability and range dynamics of invasive wasps indicate that global apiculture will likely face greater adverse impacts in the future. Therefore, our study provides important and novel information for combatting invasions of wasps and mitigating their expected impacts on global apiculture. Until now, no study has examined the future range dynamics of major invasive wasp species to assess their future impacts on global apiculture. Here, we developed 12 species distribution models to calibrate the future range dynamics of 12 major invasive Vespidae wasp species under a unified framework. An increase in their habitat suitability was identified in more than 75% of global land. Substantial range expansions were detected for all 12 species, and they were primarily induced by future climate changes. Notably, Polistes dominula and Vespa crabro had the largest potential ranges under all scenarios, suggesting their greater impact on global apiculture. Polistes chinensis and Vespa velutina nigrithorax had the highest range expansion ratios, so they warrant more urgent attention than the other species. Polistes versicolor and P. chinensis are expected to exhibit the largest centroid shifts, suggesting that substantial shifts in prioritizing regions against their invasions should be made. Europe and the eastern part of the USA were future invasion hotspots for all major invasive wasp species, suggesting that apiculture might face more pronounced threats in these regions than in others. In conclusion, given their substantial range shifts, invasive wasps will likely have increasingly negative impacts on global apiculture in the future. [ABSTRACT FROM AUTHOR]