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13 results on '"Zha Wei-bin"'

6. Ginsenoside 20(S)-Rh2 promotes cellular pharmacokinetics and intracellular antibacterial activity of levofloxacin against Staphylococcus aureusthrough drug efflux inhibition and subcellular stabilization

Author

Chen, Xiao-yang, Qian, Fei, Wang, Yao-yao, Liu, Yan, Sun, Yuan, Zha, Wei-bin, Hao, Kun, Zhou, Fang, Wang, Guang-ji, and Zhang, Jing-wei

Abstract

Intracellular Staphylococcus aureus(S. aureus) often causes clinical failure and relapse after antibiotic treatment. We previously found that 20(S)-ginsenoside Rh2 [20(S)-Rh2] enhanced the therapeutic effect of quinolones in a mouse model of peritonitis, which we attributed to the increased concentrations of quinolones within bacteria. In this study, we investigated the enhancing effect of 20(S)-Rh2 on levofloxacin (LVF) from a perspective of intracellular bacteria. In S. aureus 25923-infected mice, coadministration of LVF (1.5 mg/kg, i.v.) and 20(S)-Rh2 (25, 50 mg/kg, i.g.) markedly increased the survival rate, and decreased intracellular bacteria counts accompanied by increased accumulation of LVF in peritoneal macrophages. In addition, 20(S)-Rh2 (1, 5, 10 μM) dose-dependently increased the uptake and accumulation of LVF in peritoneal macrophages from infected mice without drug treatment. In a model of S. aureus 25923-infected THP-1 macrophages, we showed that 20(S)-Rh2 (1, 5, 10 μM) dose-dependently enhanced the intracellular antibacterial activity of LVF. At the cellular level, 20(S)-Rh2 increased the intracellular accumulation of LVF by inhibiting P-gp and BCRP. PK–PD modeling revealed that 20(S)-Rh2 altered the properties of the cell but not LVF. At the subcellular level, 20(S)-Rh2 did not increase the distribution of LVF in lysosomes but exhibited a stronger sensitizing effect in acidic environments. Molecular dynamics (MD) simulations showed that 20(S)-Rh2 improved the stability of the DNA gyrase–LVF complex in lysosome-like acidic conditions. In conclusion, 20(S)-Rh2 promotes the cellular pharmacokinetics and intracellular antibacterial activities of LVF against S. aureusthrough efflux transporter inhibition and subcellular stabilization, which is beneficial for infection treatment.

Published
2020
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7. Organic solvent extraction and metabonomic profiling of the metabolites in erythrocytes

Author

Liu Linsheng, Gu Shenghua, Ren Hong-can, Ren Meiting, Zha Wei-bin, Yan Bei, Huang Qing, Guangji Wang, Jiye A, Zhang Ying, and Sheng Longsheng

Subjects
Adult, Male, Erythrocytes, Metabolite, Clinical Biochemistry, Mass spectrometry, Biochemistry, High-performance liquid chromatography, Gas Chromatography-Mass Spectrometry, Analytical Chemistry, chemistry.chemical_compound, Liquid chromatography–mass spectrometry, medicine, Metabolome, Humans, Metabolomics, Chromatography, Chemistry, Methanol, Cell Biology, General Medicine, Red blood cell, medicine.anatomical_structure, Solvents, Gas chromatography–mass spectrometry, Intracellular
Abstract

We used erythrocytes as the model tissue to evaluate an optimal solution for the extraction of intracellular metabolites and time-dependent variation of the metabolome in living cells. Projection to latent structure (PLS) of the GC/MS and LC/MS data suggested that the most efficient solution for the extraction of metabolites from wet erythrocytes (50 mg) could be a methanol-chloroform-water mixture (950 microL, 700:200:50, v/v/v). PLS-discriminant analysis (DA) clearly profiled a time-dependent alternation of metabolic phenotype of erythrocytes. Identification of the metabolites showed that the process was characterized by accumulating of metabolic products and depleting of nutritious substances in erythrocytes during incubation.

Published
2008

8. Pharmacokinetic interactions between 20(S)-ginsenoside Rh2 and the HIV protease inhibitor ritonavir in vitro and in vivo

Author

Shi, Jian, primary, Cao, Bei, additional, Zha, Wei-bin, additional, Wu, Xiao-lan, additional, Liu, Lin-sheng, additional, Xiao, Wen-jing, additional, Gu, Rong-rong, additional, Sun, Run-bin, additional, Yu, Xiao-yi, additional, Zheng, Tian, additional, Li, Meng-jie, additional, Wang, Xin-wen, additional, Zhou, Jun, additional, Mao, Yong, additional, Ge, Chun, additional, Ma, Ting, additional, Xia, Wen-juan, additional, Aa, Ji-ye, additional, Wang, Guang-ji, additional, and Liu, Chang-xiao, additional

Published
2013
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12. [Evaluation of myocardial ischemia rat model based on metabonomic method of small molecule metabolites of plasma and cardiac muscle].

Author

Yan B, Ji-Ye A, Hao HP, Wang GJ, Liu LS, Zha WB, Zhang Y, and Gu SH

Subjects
Animals, Creatine Kinase blood, Energy Metabolism, Isoproterenol, Lipid Metabolism, Male, Myocardial Ischemia blood, Myocardial Ischemia chemically induced, Oxidative Stress, Random Allocation, Rats, Rats, Sprague-Dawley, Metabolome, Metabolomics methods, Myocardial Ischemia metabolism, Myocardium metabolism
Abstract

Isoproterenol (ISO)-induced myocardial ischemia animal model has been widely applied to the study of myocardial ischemia and evaluation of drug efficacy. Metabolic profiling of endogenous compounds can make a deep insight into biochemical process of the ISO-induced myocardial ischemia rats. Herein, rats were treated with ISO (2 mg x kg(-1)) for 10 days. After the model was established by measuring myocardial histopathology and plasma creatine kinase, GC/TOF-MS was used to determine endogenous metabolites in plasma and cardiac muscle of rats, and pattern recognition was used to process the data. Results showed that the plasma metabolic profiling of ISO-induced myocardial ischemia rats was significantly different from that of the control, and it had the tendency to the normal state after the discontinue of ISO injection. Besides, the cardiac muscle of rats treated with ISO for 10 days and the normal cardiac muscle could also be separated clearly. The potential biomarkers in plasma and cardiac muscle of model rats had homogeneity and their own specialty. Biochemical metabolic pathway analysis indicated that this myocardial ischemia model was involved in the alternation of energy metabolism, saccharometabolism, lipid metabolism, nucleoside metabolism and amino acid metabolism, and in relationship with oxidative stress. These findings revealed that metabonomics may be a promising tool to evaluate myocardial ischemia rat model induced by ISO and could further extend the study of pharmacodynamic action of drugs at the molecular level.

Published
2013

13. [Metabonomic phenotype of "formula corresponding to pattern types" based on "qi and yin deficiency pattern" of myocardial ischemia rat model].

Author

Yan B, A JY, Hao HP, Wang GJ, Liu LS, Zha WB, Zhang Y, and Gu SH

Subjects
Animals, Anti-Arrhythmia Agents pharmacology, Drug Combinations, Drugs, Chinese Herbal administration & dosage, Drugs, Chinese Herbal isolation & purification, Gas Chromatography-Mass Spectrometry methods, Injections, Male, Metabolomics methods, Myocardial Ischemia blood, Myocardial Ischemia pathology, Panax chemistry, Plants, Medicinal chemistry, Propranolol pharmacology, Rats, Rats, Sprague-Dawley, Salvia chemistry, Schisandraceae chemistry, Drugs, Chinese Herbal pharmacology, Endothelin-1 blood, Medicine, Chinese Traditional, Myocardial Ischemia metabolism, Qi, Yin Deficiency metabolism
Abstract

In order to explore the scientific connotation of "Fangzhengduiying (formula corresponding to pattern types)", "Qiyinliangxuzheng (Qi and Yin deficiency pattern)" of myocardial ischemia rat model and GC-TOF/MS based metabonomic method were used for comparing the effects of Sheng-mai injection, Salvia injection and propranolol in the present study. After data processing and pattern recognition, Sheng-mai injection showed better efficacy than the other two drugs in accordance with not only visual observation from PLS-DA scores plots but also the number of abnormal endogenous compounds restored to the normal level. Further studies showed that Sheng-mai injection could normalize the level of plasma endothelin-1, the index related to cardiovascular diseases and sleep disorders, which verified the results of metabonomics. Finally, the regulated metabolites and related metabolic pathways were analyzed, and it was supposed that the effects of Sheng-mai injection involved in the alternation of energy metabolism, lipid metabolism, amino acids metabolism, and so on. These findings provided scientific evidence to Shengmai "Fang" used for "Qi and Yin deficiency pattern" correspondingly, indicating that metabonomics has great potential in traditional Chinese medical research, which provides a novel approach and way to modernization of traditional Chinese medicine.

Published
2011

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