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The article presents a study which examines the effects of Zhukangsan, a compound traditional Chinese medicine, on cell factor in weaned piglets.

The article offers information on a study which investigates the effects of Qinkangsan Chinese medicine on changes in blood immune globulin of chickens with Newcastle disease virus (NDV).

The article discusses a study on the changes of T lymphocyte subsets by complex Chinese crude drug Qinkangsan on chicken artificially challenged by Newcastle disease virus-F48E9 (NDV-F48E9). The concentration of CD3 +, CD4+ and CD8+ subsets in peripheral blood were measured by using flow cytometry. It mentions that Quinkangsan could promote the growth of T lymphocytes and increase cell immune capability of poultry.

The article focuses on a study related to determining the effect of insulin (INS) secretion and glucose (GLU) level on the abundance of fatty acid synthase messenger RNA in vitro culture bovine adipocyte. Topics discussed include the importance of fluorescent quantitative polymerase chain reaction to detect messenger RNA, analysis of stimulating role of insulin and inhibitory role of glucose, and media culture of primary monolayer culture calf adipocytes.

The article offers information on protection of the "Qinkangsan," a Chinese herbal medicine from duck plague virus in duck embryo and duck embryo fibroblasts.

The article presents a study on the impact of Zhukangsan, a compound traditional Chinese medicine, on the cellular immune function and growth performance of wearing piglets.

The article presents a study which evaluates the effect of insulin-like growth factor-I gene by insulin and glucagon in cultured bovine hepatocytes through the use of polymerase chain reaction (PRC).

The article offers information on the effects of boron anti black bone broiler copper poisoning into the liver of chickens in China. It states that the impact is diagnosed by determining the liver function indexes and the copper content in liver. The study shows that boron added in corn-soybean diet of black bone broiler can reduce copper content in liver that it can protect liver cells from damaging.

The article discusses a study on the outcome of boron supplement on the hepatic copper content and liver function in black bone broilers suffering from copper poisoning.

The article presents a research which determines the possible effect of increasing boron anti copper poisoning to the copper content of the black bone chicken's routine blood indexes.

Resistin is a new adipokine found in vivo in recent years. Recent studies have indicated that resistin contributes to the development of insulin resistance and type 2 diabetes mellitus (T2DM) and mediates inflammatory reaction via different signal pathways. As a signal factor between inflammation and metabolism, resistin is expected to provide new insights for the treatment of insulin resistance and T2DM. However, because specific receptor of resistin has not been identified in the body so far, the molecular mechanism of resistin actions is still unclear. In this article, we review the latest progresses of resistin study, especially the role of resistin in insulin resistance and its signaling mechanism.

The purpose of this study is trying to analysis the homology between four lentogenic Class I genotype 3 Newcastle disease virus isolates from different hosts with NDV strain NDV 08-004, which was the first obtained complete genome sequence virus of class I genotype 3. The full-length genome of NDV isolates, JS/3/09/Ch, ZJ/3/10/Ch, AH/2/10/Du and JS/9/08/Go,were determined by RT-PCR and then an alyzed. All the genomes are 15 198 nucleotides (nt) in length. Compared with the full genome sequences of Class II NDV stains (genotype IV-IX),four isolates has a 6-nt deletion in the non-coding region of nuclear phosphoprotein gene between nucleotides 1 640-1 641 and 12-nt insertion in the coding region of phospho protein gene between nucleotides 2 381-2 382. All the isolates have the motifs 112EQ/RQE/GRL117 at the cleavage site of the fusion protein, which is typical of lenogenic NDV strains, and it is in agreement with the result of pathogenic tests. The full-length genome of 4 genotype 3 NDV isolates shared 93% nucleotide identity with NDV08-004. The results of alignment of 6 viral genes showed that NP gene shared the highest identity (98.3%-96.4%) and P gene shared the lowest identity (96.1%-91.9%). The results show the following two points. First, it is concluded that the isolates from different hosts share the same genotype has the insignificant divergence in the genetic information. Second, it is proposed that the mutation rates of NP/F/L genes are lower than P/M/HN genes.

On the basis of the reported amino acid sequence of alpha-bungarotoxin (alpha-BGT), DNA sequence of alpha-BGT was deduced and fourteen partially complementary oligonucleotides were designed and synthesized. A plasmid carrying the coding region of alpha-BGT was obtained by primer extension, PCR and ligation with pMD-18-T. The target fragment was digested with Xba I and EcoR I, recovered and ligated with pET28a(+). The resultant expression vector was transformed into BL21 (DE3), BL21 (DE3) Codon plus, and BL21 (DE3) plysS, respectively. Recombinant alpha-BGT was expressed in BL21 (DE3) and was analyzed by 15% Tris/tricine SDS-PAGE. The result showed that the recombinant protein, mostly found in inclusion bodies, accounted for 11.98% of the total bacterial lysate. The expression capacity could be increased to 16.28% by optimizing expression conditions. Western blotting results showed that the expressed protein had similar immunogenicity with the natural alpha-BGT protein purified from the venom of Krait Bungarus spp. In vivo toxicity assay of purified and renatured proteins in mice showed that LD50 was about 1.28 microg/g.