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Abstract Percutaneous image-guided ablation techniques are a consolidated therapeutic alternative for patients with high preoperative surgical risk for the management of oncological diseases in multiple body districts. Each technique has both pros and cons according to the type of energy delivered, mechanism of action, and site of application. The present article reviews the most recent literature results on ablation techniques applied in the field of genitourinary diseases (kidney, adrenal glands, prostate, and uterus), describing the advantages of the use of each technique and their technical limitations and summarizing the major recommendations from an international consensus meeting. Critical relevant statement The article critically evaluates the efficacy and safety of ablation therapies for various genitourinary tract diseases, demonstrating their potential to improve patient outcomes and advance clinical radiology by offering minimally invasive, effective alternatives to traditional surgical treatments. Key Points Ablation therapies are effective alternatives to surgery for renal cell carcinoma. Ablation techniques offer effective treatment for intermediate-risk prostate cancer. Ablation is a promising tool for adrenal tumor management. Ablation reduces fibroid symptoms and volume, offering an alternative to surgery. Graphical Abstract

Objective To explore effective treatments and prognostic factors for diffuse intrinsic pontine glioma (DIPG). Methods Clinical and imaging information and survival data of 14 DIPG patients, treated with radiotherapy combined with temozolomide and nitolizumab or radiotherapy combined with ACT001, were retrospectively analysed at Beijing Tiantan Hospital, Capital Medical University from April 2021 to January 2024. The median progression free survival (PFS) and overall survival (OS) were calculated using Kaplan - Meier survival curves, and multifactorial Cox regression analysis was used to investigate the effects of different factors on PFS and OS. Results The objective response rate (ORR) was 10/14, and the median PFS and OS were 7.83 and 8.30 months, respectively. Multfactorial Cox regression analysis identified the absence of enhancement on baseline imaging as a good prognostic variable for both PFS (RR = 0.052, 95%CI: 0.006-0.416; P = 0.005) and OS (RR = 0.046, 95%CI: 0.005-0.413; P = 0.006), while male (RR = 0.085, 95%CI: 0.009-0.764; P = 0.028), older age (RR = 0.631, 95%CI: 0.423-0.942; P = 0.024), and the absence of symptoms of cranial nerve involvement at the onset (RR = 0.116, 95%CI: 0.017- 0.781; P = 0.027) were also good prognostic variables for OS. Conclusions Female, younger age at diagnosis, cranial nerve involvement at the onset, and enhancement on baseline imaging are risk factors for the survival of children with DIPG.

In the development of carbon nanotube (CNT)-reinforced cement-based matrices, one of the fundamental issues that investigators are confronting is CNT/cement-based matrix interfacial bonding, which determines the load transfer capability from the matrix to the CNT. In the present work, the stress transfer properties of multi-walled carbon nanotubes (MWCNTs) and ultralight foamed concrete matrices were studied using microscopic Raman spectrometry analysis. Two types of CNTs, such as MWCNT and MWCNT-COOH, were considered, wherein MWCNT-COOH was covered with fundamental COOH groups. The results show that the compressive and flexural strengths were 75 and 236% better for ultralight foamed concrete with a dry density of 200 kg/m3 with 0.4 wt% MWCNT-COOH addition, respectively. This indicates that the fundamental COOH groups of the MWCNT play an important role in determining the interfacial bonding characteristics between the MWCNT and the ultralight foamed concrete matrix. Therefore, the attachment of COOH groups with a reasonable concentration to the MWCNT surface may be an effective way to significantly improve the load transfer between the MWCNT and the ultralight foamed concrete matrix, leading to increased compressive and flexural strength values of composites.

Keratoconus is a progressive disease that seriously affects the eyesight of young people. Keratoconus often causes high myopia, irregular astigmatism, and some patients with advanced corneal scar, visual acuity significantly decreased. In the course of diagnosis and treatment of keratoconus, refractive reconstruction of keratoconus is the focus of clinical attention. After years of research and exploration, a variety of treatment methods in different stages of disease(non-progressive, progressive, advanced)for keratoconus patients refractive reconstruction, so that they can obtain good visual function. This paper mainly reviews the refractive reconstruction schemes of keratoconus in different periods, analyzes the advantages and disadvantages of various treatment schemes at home and abroad, and summarizes the characteristics of different treatment methods, with a view to providing theoretical reference and new treatment ideas for the diagnosis and treatment of keratoconus.

Purposes Aiming at the problem that Granger Causality (GC) analysis in EEG emotion recognition does not consider the interaction between nodes when constructing brain network, a method of constructing brain network by combining fuzzy cognitive map (FCM) and GC analysis is proposed. Methods First, on the basis of the correspondence between the structure of FCM and GC brain network, the GC brain network is modeled and improved by using the causal attributes among FCM nodes, and the FCM-GC brain network is constructed, by taking into account the cooperative interaction among nodes. Furthermore, in order to deeply integrate FCM with GC brain network, the spatial position information of EEG electrode channel is added to FCM training, and a new IFCM-GC brain network is constructed. On the basis of DEAP emotional EEG database, the features of IFCM-GC brain network are extracted, and the support vec tor machine is used as the recognition model. The average recognition rates in valence dimension and incentive dimension are 97.10% and 97.00%, respectively, which are more than 8% higher than the existing research on GC improvement. Findings The GC brain network constructed by this method takes into account the cooperative interaction among multiple nodes, and effectively improves the performance of emotion recognition system.

In this article, we consider the following quasilinear Schrödinger system: −εΔu+u+k2ε[Δ∣u∣2]u=2αα+β∣u∣α−2u∣v∣β,x∈RN,−εΔv+v+k2ε[Δ∣v∣2]v=2βα+β∣u∣α∣v∣β−2v,x∈RN,\left\{\begin{array}{ll}-\varepsilon \Delta u+u+\frac{k}{2}\varepsilon \left[\Delta \hspace{-0.25em}{| u| }^{2}]u=\frac{2\alpha }{\alpha +\beta }{| u| }^{\alpha -2}u{| v| }^{\beta },& x\in {{\mathbb{R}}}^{N},\\ -\varepsilon \Delta v+v+\frac{k}{2}\varepsilon \left[\Delta \hspace{-0.25em}{| v| }^{2}]v=\frac{2\beta }{\alpha +\beta }{| u| }^{\alpha }{| v| }^{\beta -2}v,& x\in {{\mathbb{R}}}^{N},\end{array}\right. where ε>0,k

【Objective】In Space Division Multiplexing (SDM) system, in order to achieve mode demultiplexing, it is necessary to adopt Multiple Input Multiple Output (MIMO) equalization scheme at the receiving end. As the number of transmission modes or transmission capacity increases, the computational complexity of MIMO increases significantly, resulting in high power consumption. Therefore, it is necessary to reduce the computational complexity of MIMO equalization.【Methods】We propose a frequency-domain joint equalization technique based on Cyclic Prefix (CP) to reduce the complexity compared with a traditional frequency-domain independent equalization and a separate dispersion compensation module in few-mode fiber short-range transmission systems.【Results】Based on the few-mode fiber model in VPI simulation platform, we build a 6×6 few-mode fiber transmission system to verify the advantage of computational complexity reduction of the proposed technology. The simulation results show that, the performance and computational complexity reduction of different CP ratios are also different. Under the same simulation conditions, when the proportion of CP is larger, the reduction of computational complexity is also larger, vice versa. With the same performance, the computational complexity of the frequency-domain equalization scheme with CP accounting for 11.11%is only 15.29%of that of the traditional frequency-domain equalization scheme based on block convolution.【Conclusion】Compared with the traditional frequency-domain equalization combined with a separate dispersion compensation module, the proposed frequency-domain equalization with CP has lower computational complexity.

Diabetic cardiomyopathy (DCM) is identified as a progressive disease that may lead to irreparable heart failure. Toll-like receptor (TLR) signaling is believed to be implicated in the pathogenesis of DCM. This study intended to explore the potential impact of Toll-like receptor 4 (TLR4) on DCM in vitro and in vivo. Streptozotocin and HG medium were utilized to induce diabetes in animal and cell models, respectively. Selective TLR4 inhibitor TAK-242 and calcium/calmodulin-dependent protein kinase-II (CaMKII) inhibitor KN-93 were employed to explore the involvement of TLR4/CaMKII in DCM. TLR4 expression was increased in DCM hearts, while inhibition of TLR4 activation by TAK-242 improved cardiac function, attenuated heart hypertrophy, and fibrosis, as well as reduced oxidative stress and proinflammatory cytokine levels in rats, which were confirmed by Doppler echocardiography, hematoxylin and eosin staining, and Masson Trichome staining and specific enzyme-linked immunosorbent assay kits. Besides, the expression of hypertrophy-related molecules and oxidative stress damage were also inhibited by TAK-242. Furthermore, TAK-242 treatment reduced CaMKII phosphorylation accompanied by decreased expression of NOD-like pyrin domain-containing protein 3, gasdermin D (GSDMD), The N-terminal domain of Gasdermin D (GSDMD-N), apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) and Caspase-1 both in vivo and in vitro. Similar positive impacts on HG-induced pyroptosis were also observed with KN-93 treatment, and this was achieved without affecting TLR4 expression. Collectively, our work suggested that TAK-242 demonstrated substantial benefits against DCM both in vivo and in vitro, potentially attributed to the suppression of the TLR4-mediated CaMKII/NLRP3 pathway activity.

Litter input is a critical link between the circulation and flows of energy and matter in forest ecosystems. Drought events impact litter inputs, affecting forest ecosystems’ nutrient and energy cycles. To investigate the response of litter production to extreme drought, explore their recovery mechanism, and the difference between whole year, concurrent drought, non-drought period, rainy season, and dry season. We studied an extreme drought event in a subtropical evergreen broadleaved forest in the Ailao Mountain in 2009–2010. We used 10 years (2005–2014) of monthly litter production and climate data, including monthly average precipitation (MAP), soil water content (SWC), and monthly average temperature (MAT). The observation period was separated into pre-drought (2005–2008), during-drought (2009–2010), and post-drought (2011–2014) periods. We found that annual total litter production decreased by 12 % during the drought (p

High-intensity land development has led to numerous ecological problems, severely threatening regional sustainable development. Resilience is key to maintaining regional sustainable development. This study focuses on the Taohe River Basin and constructs an ecological resilience evaluation model based on the framework of ecosystem adaptability, ecosystem resistance, and ecosystem recovery from the perspective of resilient ecosystem functions. Using software such as InVEST and Fragstats, the study assesses the spatiotemporal changes in ecological resilience in the Taohe River Basin. Based on the ecological resilience pattern, ecological sources were identified, resistance surfaces were constructed, and ecological corridors were extracted, thereby establishing an ecological resilience security pattern for the Taohe River Basin. During the study period, the level of ecological resilience in the Taohe River Basin showed a gradual upward trend. A total of 33 ecological source areas were identified, accounting for 27.57 % of the total area of the Taohe River Basin. Seventy ecological corridors were identified, with a total length of 6478.63 km and an average length of 92.55 km. Combining the topography, ecological background conditions, and current urban development status of the Taohe River Basin, an ecological security pattern of “two screens, two corridors, five areas, and multiple points” was constructed, along with corresponding optimization suggestions. The research results are of great significance for optimizing spatial layout, maintaining regional ecological security, and promoting regional integrated development. Additionally, they provide new ideas for assessing regional ecological resilience.

ObjectiveTo investigate the trends and significant determinants of delayed HIV diagnosis （DHD） among newly reported HIV/AIDS cases in Xuhui District， Shanghai between 2018 and 2022.MethodsIn the newly reported HIV/AIDS cases， patients died within one year without accident， HIV/AIDS cases with CD4 cell count

Angelica dahurica is a common species of medicine and food homology, which is not only a common clinical traditional Chinese medicine, but also a spice, with a wide range of uses. In order to obtain the whole genome sequence information of A. dahurica, we used A. dahurica var. formosana leaf DNA as material, and the Nanopore sequencing technology was used to establish its nucleotide sequences database, then genome assembly, function annotation and evolution analysis were carried out by bioinformatic methods. The results were as follows:(1) A total of 662 Gb of the third-generation data were obtained after fittering the original sequencing data, with the Read N50 about 32 932 bp. The assembled A. dahurica genome size was 5.6 Gb, Contig N50 was about 806 638 bp. (2) The genes were with gene annotations accounted for 66.47% after being compared with functional databases such as NR, KOG and KEGG. The result of KOG gene annotation was that the protein function of A. dahurica concentrated in the general functional prediction, posttranslational modification, protein turnover, chaperones and signal transduction mechanisms. GO functional classification indicated that the genes of A. dahurica concentrated on cell biological processes and components. KEGG analysis found that the A. dahurica genes mostly involved in metabolic pathways. (3) And 45 genes of BGLU family were identified in A. dahurica. In this study, the whole genome of A. dahurica is resolved by the third-generation sequencing technology for the first time, which provides important theoretical references for the systematic biological study and the further study of the function of BGLU in the growth and development of A. dahurica.

A pilot plant of microbubble ozonation and biological treatment was used to treat chemical wastewater in depth, and its long-term operation performance was evaluated and predicted by a neural network model. The results showed that the chemical wastewater could be treated stably and effectively in the pilot plant, and the average removal rates of COD and UV254 in the phase of microbubble ozontion were 35.1% and 52.5%, respectively. The mass ratio of ozone dosage to influent COD amount was an important parameter influencing the operation performance, which should be controlled as 0.5 for better performance. The wastewater biodegradability could be improved effectively by microbubble ozonation, BOD5/COD could increase from 0.10 to 0.33 and the ratio of BOD5 generated amount to COD removed amount could reach to 37.23%. Then biological treatment was applied for the improved water, and the contribution of BOD5 removal to COD removal was more than 80% in biological treatment. Back propagation neural network (BPNN) was used to establish a prediction model of the effect of m on COD removal performance in microbubble ozonation, which showed a stable prediction ability with acceptable accuracy.

Frequency Diverse Array (FDA) is a brand-new electronically scanned radar system. The time-range-angle three-dimensional correlation characteristics of its beampattern have broad application prospects in the fields of electronic countermeasures and security communications. One-dimensional uniform linear FDA array (ULFDA) has the problem of angle-range coupling in the transmit beampattern. To solve this problem, this paper adopts sine based on the symmetrical subarray density taper array structure. The frequency offset obtains the dot-shaped beampattern pointing control method at the target point position. In addition, we analyze the time-varying problem of the FDA pattern, and get the conclusion that the mainlobe of the density tapered array sin-FDA based on the subarray structure has a fixed direction in the angle dimension and changes in the range dimension.

According to 2019 statistical data, the nationwide surgical volume reached 69.3 million patient cases, with pulmonary surgeries constituting a vital component and showing an annual increase. However, as the number of surgeries rises, so does the incidence of perioperative complications, including thrombosis, which adversely affects patient outcomes. Therefore, understanding of the causes, risk factors, and mechanisms of thrombosis in the context of pulmonary surgeries, as well as implementing effective preventive measures, can mitigate the adverse effects of thrombosis related to pulmonary surgeries. This article discusses the incidence, risk factors, clinical manifestations, treatment, and prognosis of postoperative thrombosis in pulmonary surgeries, aiming to provide new insights for related clinical research.

Diabetic retinopathy (DR) is a retinal disease that arises from impaired glucose tolerance and leads to retinal microvascular leakages. Recent studies have indicated that DR pathogenesis is linked to dysfunctional retinal pigment epithelial (RPE) cells.

In order to improve the dewatering performance of waste activated sludge，iron-based sludge biochar（Iron-SBC） activating persulfate（PDS） was used to treat sludge. The effects of PDS dosage，Iron-SBC dosage，reaction time，reaction temperature and initial pH on waste activated sludge dewatering efficiency were studied，and the mechanism was also analyzed. The results showed that when the temperature was 65 ℃，the dosage of PDS per unit TSS was 150 mg/g，the dosage of Iron-SBC per unit TSS was 350 mg/g，the initial pH was 6.68，after being conditioned by Iron-SBC/PDS for 20 minutes，the sludge capillary water absorption time，sludge specific resistance and sludge cake moisture content decreased to 8.4 s，5.4×1012 m/kg and 73.5%，respectively. The mechanism analysis indicated that oxidation reaction occurred during the process. The originally tight and flat sludge flocs and extracellular polymers were cracked and the bound water was released. The highly hydrophilic tightly bound extracellular polymeric substances（TB-EPS） in sludge were transformed into loosely bound extracellular polymeric substances（LB-EPS） and soluble extracellular polymeric substances（S-EPS），and the proteins which were unfavorable to sludge dewatering were oxidized and degraded. The decrease of TB-EPS and neutralization of positive iron ions increased the Zeta potential value. Iron-SBC with rigid structure reduced the compressibility coefficient of sludge cake. At the same time，under the flocculation of Fe3+，the fractal dimension of sludge became larger. Under the coupling action of “oxidation-skeleton construction”，the waste activated sludge deep dewatering was realized.

ObjectiveTo provide technical support for the molecular surveillance of pathogenic bacteria strains carrying mobile colistin resistance-1 （mcr⁃1） gene isolate from inlet of wastewater treatment plants （WWTP）.MethodsThe Enterobacteriaceae strains carrying mcr⁃1 resistance gene isolate from inlet of WWTP during April 1 to June 30， 2023 in Shanghai were cultured on blood-rich and SS culture medium and were identified using a mass spectrometry analyzer. The mcr⁃1 gene and copy number were detected by real-time fluorescence quantitative PCR. Drug susceptibility test was performed by microbroth dilution method. The copy numbers of Escherichia coli carrying mcr⁃1 gene isolated from wastewater and human fecel were statistically analyzed by SPSS 25.0.ResultsA total of 14 strains carrying the mcr⁃1 gene were isolated from 49 WWTP samples， and the positive isolation rate was 28.6%， including 12 non-diarrheal E. coli strains and 2 Klebsiella pneumoniae strains. The drug susceptibility results showed that all 14 strains were multi-drug resistant bacteria. They were all sensitive to imipenem and tigecycline， but were ampicillin- and cefazolin-resistant. There was no significant difference in the copy number between human-sourced diarrheal E. coli and wastewater-sourced non-diarrheal E. coli （t=0.647， P>0.05）.ConclusionThe isolation and identification of strains carrying the mcr⁃1 gene from inlet of WWTP samples were firstly established in Shanghai. The multi-drug resistance among the isolated strains is severe. To effectively prevent and control the spread of colistin-resistant bacteria， more attention should be paid to the surveillance of mcr⁃1 gene.

ObjectiveThe widespread prevalence of sclerotinia disease poses a significant challenge to the cultivation and supply of oilseed rape, not only results in substantial yield losses and decreased oil content in infected plant seeds but also severely impacts crop productivity and quality, leading to significant economic losses. To solve the problems of complex operation, environmental pollution, sample destruction and low detection efficiency of traditional chemical detection methods, a Bi-directional Gate Recurrent Unit (Bi-GRU) model based on space-spectrum feature fusion was constructed to achieve hyperspectral images (HSIs) segmentation of oilseed rape sclerotinia infected area.MethodsThe spectral characteristics of sclerotinia disease from a spectral perspective was initially explored. Significantly varying spectral reflectance was notably observed around 550 nm and within the wavelength range of 750-1 000 nm at different locations on rapeseed leaves. As the severity of sclerotinia infection increased, the differences in reflectance at these wavelengths became more pronounced. Subsequently, a rapeseed leaf sclerotinia disease dataset comprising 400 HSIs was curated using an intelligent data annotation tool. This dataset was divided into three subsets: a training set with 280 HSIs, a validation set with 40 HSIs, and a test set with 80 HSIs. Expanding on this, a 7×7 pixel neighborhood was extracted as the spatial feature of the target pixel, incorporating both spatial and spectral features effectively. Leveraging the Bi-GRU model enabled simultaneous feature extraction at any point within the sequence data, eliminating the impact of the order of spatial-spectral data fusion on the model's performance. The model comprises four key components: an input layer, hidden layers, fully connected layers, and an output layer. The Bi-GRU model in this study consisted of two hidden layers, each housing 512 GRU neurons. The forward hidden layer computed sequence information at the current time step, while the backward hidden layer retrieves the sequence in reverse, incorporating reversed-order information. These two hidden layers were linked to a fully connected layer, providing both forward and reversed-order information to all neurons during training. The Bi-GRU model included two fully connected layers, each with 1 000 neurons, and an output layer with two neurons representing the healthy and diseased classes, respectively.Results and DiscussionsTo thoroughly validate the comprehensive performance of the proposed Bi-GRU model and assess the effectiveness of the spatial-spectral information fusion mechanism, relevant comparative analysis experiments were conducted. These experiments primarily focused on five key parameters—ClassAP(1), ClassAP(2), mean average precision (mAP), mean intersection over union (mIoU), and Kappa coefficient—to provide a comprehensive evaluation of the Bi-GRU model's performance. The comprehensive performance analysis revealed that the Bi-GRU model, when compared to mainstream convolutional neural network (CNN) and long short-term memory (LSTM) models, demonstrated superior overall performance in detecting rapeseed sclerotinia disease. Notably, the proposed Bi-GRU model achieved an mAP of 93.7%, showcasing a 7.1% precision improvement over the CNN model. The bidirectional architecture, coupled with spatial-spectral fusion data, effectively enhanced detection accuracy. Furthermore, the study visually presented the segmentation results of sclerotinia disease-infected areas using CNN, Bi-LSTM, and Bi-GRU models. A comparison with the Ground-Truth data revealed that the Bi-GRU model outperformed the CNN and Bi-LSTM models in detecting sclerotinia disease at various infection stages. Additionally, the Dice coefficient was employed to comprehensively assess the actual detection performance of different models at early, middle, and late infection stages. The dice coefficients for the Bi-GRU model at these stages were 83.8%, 89.4% and 89.2%, respectively. While early infection detection accuracy was relatively lower, the spatial-spectral data fusion mechanism significantly enhanced the effectiveness of detecting early sclerotinia infections in oilseed rape.ConclusionsThis study introduces a Bi-GRU model that integrates spatial and spectral information to accurately and efficiently identify the infected areas of oilseed rape sclerotinia disease. This approach not only addresses the challenge of detecting early stages of sclerotinia infection but also establishes a basis for high-throughput non-destructive detection of the disease.

Objective To explore the effects of prohibitin 2(PHB2) on sensitivity of non-small cell lung cancer cell line A549 to erlotinib (Erl) and its potential mechanisms. Methods RACK1-specific small interfering RNA was transfected in A549 cells for knocking-down of PHB2. The effects of PHB2 inhibition on cell proliferation and apoptosis induced by Erl were observed. The colocalization of microtuble-associated protein light chain 3 alpha (LC3) and mitochondria was visualized by MitoTracker staining and green fluorescent protein-microtuble-associated protein light chain 3 alpha (GFP-LC3) transfection. Cell proliferation was detected by 5-ethynyl-2′-deoxyuridine (EdU) staining. Cell colony formation was evaluated by colony forming assay. Apoptotic index of A549 cells was evaluated by TUNEL. Western blot was used to measure the expressions of PHB2 and LC3Ⅱ. Mitochondrial transmembrane potential, cytochrome c and respiratory chain complex Ⅰ/Ⅱ/Ⅴ activity were analyzed by the commercially available kits. Results Compared with the siPHB2 and siCtrl+Erl group, the EdU-positive A549 cells and the number of cell colonies decreased significantly (P＜0.05), while the TUNEL-positive A549 cells increased significantly(P＜0.05) in the siPHB2+Erl group. In addition, compared with the siPHB2 and siCtrl+Erl group, mitochondrial transmembrane potential and respiratory chain complex Ⅰ/Ⅱ/Ⅴ activity decreased significantly (all P＜0.05) and the levels of cytochrome c increased in mitochondrial fractions (P＜0.05) and decreased in cytosolic fractions(P＜0.05) in the siPHB2+Erl group. Conclusions PHB2 inhibition significantly improves sensitivity of A549 cells to Erl,which may be explained by inhibition of PHB2-mediated mitochondrial autophagy.

To clarify the chemical constituents of the traditional Chinese medicine Aloe made from Aloebarbadensis, i.e., the concentrated dry matter of the juice of the leaves of A.barbadensis, a systematic qualitative analysis of them was conducted using the technique of HPLC-DAD-ESI-IT-TOF-MSn in conjunction with the comparison of reference compounds and literature search. The gradient elution was performed with water (A)-acetonitrile (B) as mobile phase at a flow rate of 1.0 mL·min-1. The liquid chromatography-mass spectrometry data were acquired under alternate negative ion and positive ion detection mode using an ESI ion source. The structure elucidation of the chemical constituents was mainly based on negative ion mass spectrometry data. The results were as follows: (1) For the first time, the fragmentation pathways of anthraquinones (aloe-emodin, physcion, and emodin-8-O-β-D-glucoside), anthrones (aloin A, aloinoside A), chromones (aloeresin D, 7-O-methylaloeresin A, altechromone A, aloesin, aloeresin G, and aloeresin C), and α-pyranones (aloenin A, aloenin B) in Aloe made from A.barbadensis were clarified. The fragmentation pathway of anthraquinones was dominated by loss of CO2 and CO, and that of anthrones was dominated by cleavage of hexosides and loss of CO. The fragmentation pathways of chromones was dominated by cleavage of hexosides and hydrolysis of the ester group, and that of α-pyranones was dominated by cleavage of hexosides and loss of CO2 and H2O. (2) A total of 168 chemical constituents of Aloe made from A.barbadensis were detected, and 78 of them were identified on the basis of reference compound comparison, literature retrieval, and chemical database (such as SciFinder) searching. The 78 compounds included 3 anthraquinones, 29 anthrones, 35 chromones, 7 α-pyranones and 4 other constituents. Twenty-three of 78 compounds were discovered in the leaves of A.barbadensis for the first time. Fourteen of 23 were newly discovered compounds, including aloinoside D, isoeleutherin, and ethylidene-aloenin, possessed antibacterial, anti-inflammatory, or free radical scavenging activities. The results of this study further enrich the information on the chemical constituents of the traditional Chinese medicine Aloe made from A.barbadensis, and lay a foundation for the study of the therapeutic material basis and quality control methods of Aloe.

Hepatitis E virus （HEV） is a universal foodborne pathogen that infects swine， deer， rabbits， camels， and other animals. Direct contact with infected pigs and other animals or consumption of contaminated food （uncooked or undercooked） poses a risk of HEV infection in humans. This article summarizes the etiology and epidemiology of HEV， its prevalence in various animal species， and the contamination of meat and meat products， the aquatic environment， seafood， vegetables， and other crops. Therefore， people can better understand the risk of foodborne HEV transmission and provide a scientific basis for preventing and controlling its prevalence.

ObjectiveTo utilize whole genome sequencing to comprehensively compare and analyze the genomic characteristics of Listeria monocytogenes （L. monocytogenes） and Listeria innocua （L. innocua） isolated from commercially available foods in Gansu Province.MethodsDuring 2021 and 2022， 25 strains of L. monocytogenes and 7 strains of L. innocua were isolated from commercially available food sources within Gansu Province and subjected to whole genome sequencing for phylogenetic lineage， clonal complex （CC）， sequence type （ST）， virulence gene， resistance gene， and pan-genome analyses.ResultsThe 32 Listeria strains examined could be classified into three distinct groups： L. monocytogenes lineages Ⅰ and Ⅱ and L. innocua. Considering their CC types， further subdivision into 10 subgroups was observed for L. monocytogenes， whereas L. innocua had 5 subgroups. Core genome multi-locus sequence typing effectively differentiated strains with different CC types within each lineage， with lineage Ⅰ showing a closer evolutionary relationship with L. innocua. All 25 L. monocytogenes strains carried Listeria pathogenicity island 1 （LIPI-1） and internalin genes； none carried LIPI-3， and 2 strains of ST87 carried LIPI-4. Conversely， none of the seven strains of L. innocua carried LIPI-1 or internalin genes； however， they all carried LIPI-4， and 5 carried LIPI-3. Among the L. monocytogenes strains， 16 carried surface sensory island 1 （SSI-1）， and 3 carried SSI-2； all 7 L. innocua strains did not carry SSI-1， but 6 of them carried SSI-2. The pan-genome size of L. monocytogenes increased linearly with the number of sequenced genomes. Upon reaching 15 strains， the number of core genes stabilized at 2 272， accounting for 46.2% of the pan-genome genes. Furthermore， 1 487 common core genes were shared between the 25 L. monocytogenes strains and 7 L. innocua strains， with the count tending to stabilize when the strain count reached 10.ConclusionMulti-locus sequence typing of the core genome can effectively distinguish among Listeria strains originating from different lineages and CCs. The similarity in the biochemical characteristics of L. monocytogenes and L. innocua is due to their close genetic relationship， while the differences in their pathogenicity can be attributed to the presence of certain virulence genes in L. monocytogenes.

In the ground attack mission, the attack plan needs to be formulated and distributed to the attack aircraft based on the threat source intelligence data obtained in advance with the help of the tactical mission planning system. The passive radar reconnaissance and direction finding system can calculate the location information of threat sources and provide it to the task planning system by processing the received signal. When the enemy radar is a frequency diverse array system rather than a phased array system, the bending characteristics of the beam of the frequency diverse array radar can deceive the passive direction finding system. On the basis of analyzing the basic structure of frequency diverse array radar, this article compares the phase plane characteristics of phased array and frequency diverse array, and takes the interferometer direction finding system as an example to study the deception effect of frequency diverse array radar compared to phase method direction finding. The deception simulation results of several nonlinear FDA arrays show that the virtual transmitter intersection coordinate deviation of log-FDA is greater than that of sin-FDA. When the x-axis coordinate of the interferometer is xG=300 km, the virtual transmitter intersection coordinates gradually approach the actual position of the FDA array as the y-axis coordinate of the interferometer increases. The intersection offset is log-FDA>sin -FDA>reciprocal-FDA.

In order to further improve the load-bearing performance of the RV reducer, a force analysis is conducted on the cycloidal pin wheel pair, regarding the contact stress and meshing stiffness of the cycloidal pin wheel as the research objective functions. In order to optimize its load-bearing performance, a mathematical analysis model of contact stress and meshing stiffness is proposed regarding the important structural parameters of cycloidal and pin wheel, as the design variables such as needle tooth distribution circle radius, eccentricity, and needle tooth radius, VB.NET Software is applied to calculate and analyzes the influence of structural parameters of the cycloidal and pin wheel on the meshing stiffness, contact stress, meshing force, and torsional stiffness of the cycloidal and pin wheel pair. The results of calculation and simulation indicate that the meshing stiffness and torsional stiffness of cycloidal and pin wheel increase with the increase of the radius of the distribution circle and eccentricity the decrease of the radius of the teeth. The research provides theoretical foundation for the design and optimization of the structure of cycloidal and pin wheel reducers.

N6-methyladenosine (m6A) is the most prevalent modification that regulates gene expression in eukaryotes. It regulates splicing, degradation, stability, and translation of RNA. Numerous studies have demonstrated the close association between m6A methylation and tumor development, highlighting its crucial role in regulating tumor immune response. The m6A modification actively participates in governing immune cell differentiation and maturation as well as modulating anti-tumor immune responses. Within the tumor microenvironment, m6A modification can also impact the recruitment, activation, and polarization of immune cells, thereby either promoting or inhibiting tumor cell proliferation and metastasis. Consequently, it plays a pivotal role in reshaping the tumor immune microenvironment. In recent years, immunotherapy for tumors has been increasingly applied to clinical practice with notable success achieved through approaches such as immune checkpoint inhibitor therapy and adoptive cell immunotherapy. Targeting m6A modifications to interfere with the immune system, such as targeting dysregulated m6A regulators through small molecule inhibitors and inducing immune cell reprogramming, can improve anti-tumor immune response and strengthen immune cells′ ability to recognize and kill tumor cells. The m6A modification represents a novel avenue for potential clinical application within tumor immunotherapy. This review provides a comprehensive summary of the regulatory impact of m6A methylation modification on immune cells in the context of cancer, while also delving into novel targets for tumor immunotherapy.

This study aimed to compare the postoperative analgesic efficacy and motor recovery of a novel lumbar plexus block (LPB) with that of a femoral nerve block (FNB) after total knee arthroplasty (TKA). Forty patients who underwent TKA were randomised equally into an lumbar plexus and sciatic nerve (LS) group (receiving novel LPB) and an femoral and sciatic nerves (FS) group (receiving FNB). The assessed variables were the onset time of pain, time to the first analgesic request, pain scores, motor block at 6, 12, and 24 h after TKA, and the number of patients receiving successful blockade for each branch of the lumbar plexus. In the LS group, the femoral, lateral femoral cutaneous, genitofemoral, iliohypogastric, ilioinguinal, and obturator nerves were blocked in 18, 20, 16, 18, 15, and 19 patients. Compared to the FS group, the LS group had a significantly shorter onset time of pain and time to the first analgesic request, a significantly larger total postoperative dose of sufentanil, significantly higher numeric rating scale scores for both rest and dynamic pain at 6, 12, and 24 h, and faster motor recovery. Novel ultrasound-guided LPB has a high blocking success rate and provides inferior postoperative analgesia, but faster motor recovery after TKA than FNB.

Co3+-doped LaBO3 powders were synthesized by combustion method using La(NO3)3·6H2O, Co(NO3)3·6H2O), H3BO3, and C2H5NO2 as the raw materials with the mole ratio of [La(NO3)3·6H2O+Co(NO3)3·6H2O]:H3BO3:C2H5NO2=3:3:5. The synthesis process and crystal morphology of the target products La(1−x)CoxBO3 (x=0.01~0.04) at 750~950 ℃ were studied. The influence of synthesis conditions on the crystal morphology of the powder products was analyzed. The optimal ratio of the raw materials, reaction temperature, reaction time, and doping dosage were obtained. In the results, the combustion synthesis method can be used to prepare the Co3+-doped LaBO3 powders, and the experimental conditions are simple, the calcination temperature is low, the yield is high, and it is easy to industrialization. The optimal reaction condition is 900 ℃ and 4 h, the doping dosage of cobalt as modifier is x=0.03, and the products are La0.97Co0.03BO3 powders. Under the optimal reaction condition, the products are the short rod-like powders in the uniform particle size distribution with the diameter of 115~185 nm and the length of 400~600 nm. The fluorescence excitation band appears at 330~440 nm for the Co3+-doped LaBO3 powders, indicating that the Co3+-doped LaBO3 powders with the uniform particle size and crystal morphology can be used as the substrate material for preparing the luminescent powders.

Fatigue performance of RV-20E reducers is investigated experimentally herein; load and life curves of RV-20E with different crankshaft heat treatment strengthening processes are obtained. The optimum surface microstructure and optimum surface hardness of the crankshaft for improving the fatigue life of the RV-20E reducer are analyzed, and the failure mode and failure mechanism of the crankshaft of the RV-20E reducer are discussed. Based on the S-N theory and the Miner theory, and combined with the force analysis of RV-20E, the life parameter of RV-20E reducers based on the crankshaft needle roller bearing assembly is derived, and the test data is compared and analyzed. The analysis and comparison show that the life parameter can effectively correlate the fatigue life of RV-20E reducers and can be used to predict the fatigue life of RV-20E reducers.

ObjectiveTo develop an ultrahigh-performance liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS） analytical method for the determination of cyanuric acid in the outer packaging of imported cold-chain food.MethodsA 50% aqueous acetonitrile solution was used as an extraction solvent. The extraction was performed by shaking the samples. The sample was centrifuged， and the supernatant was transferred for injection. Cyanuric acid was separated using a Waters ACQUITY BEH HILIC （2.1×100 mm， 1.7 μm）. The mobile phase consisted of acetonitrile and a 10 mmol/L aqueous ammonium acetate solution. An isocratic elution was also performed. Cyanuric acid was detected using UHPLC-MS/MS.ResultsGood linearity was achieved， with a correlation coefficient above 0.999 0. The limit of detection and limit of quantitation of the method were 5.0-20.0 and 20.0-50.0 μg/kg， respectively. The mean recoveries at low， middle， and high spiked levels were 80.0%-96.6%. The intra- and inter-day relative standard deviations were

ObjectiveTo investigate the molecular and biological characteristics of Vibrio vulnificus （V. vulnificus）isolated from Guangzhou.MethodsThirty-eight strains of V. vulnificus were collected from Guangzhou， and whole-genome sequences were obtained. The population structure of V. vulnificus was inferred by utilizing 50 publicly available genome sequences obtained from NCBI. FineSTRUCTURE software was employed for this analysis. Antibiotic resistance and virulence factors were identified using CARD， ResFinder， and VFDB databases.ResultsFour well-supported phylogenetic groups or lineages （L1-L4） were identified， and all genomes of the strains in Guangzhou were classified into L1 （47%） and L2 （53%）. The predominant ST were ST357， ST157， ST136， ST139， ST345， ST303， and so on， which showed regional aggregation. Multiple genome identification of V. vulnificus revealed 11 drug resistance genes： acrF， CRP， catB9， rpoC， ugd， and others. MCR genes related to polymyxin resistance were identified in V. vulnificus for the first time. The species also carries 23 virulence genes in five classes encoding flagellin， type Ⅱ secretory system protein， capsular polysaccharide， RtxA toxin， iron overload， and other virulence-related genes.ConclusionVibrio vulnificus in Guangzhou undergoes highly homologous recombination and carries a variety of antimicrobial resistance genes and virulence-related genes. Therefore， monitoring and management of Vibrio vulnificus should be strengthened.

Aiming at the problems of information uncertainty and real-time faced by ground threat assessment during UAV air-to-ground combat, the limitations of existing threat assessment methods are analyzed, and a new threat assessment method based on improved Bayesian network is proposed in this paper. In this method, a hybrid-driven approach of threat data and expert knowledge is used to improve the rationality of threat assessment model, and a layered threat assessment architecture is constructed. Threat levels are classified based on the relationship between carrier aircraft and threat envelope, threat radiation, etc. In order to improve the rationality of the ground threat assessment model, a calculation model of ground threat intervisibility probability is established, and the intervisibility probability is taken as the input of Bayesian network threat assessment model. Simulation results show that the model has less computation and good real-time performance, and the introduction of threat intervisibility probability improves the rationality of ground threat assessment.

BackgroundQuality of life， as a major criterion for judging the clinical outcome of ovarian cancer patients， can be affected by adverse psychological symptoms of patients. Meanwhile， fear of disease progression， as a frequent psychological symptom among cancer survivors， is significantly influenced by metacognition， while there is a paucity of research into the specific correlation among the three in patients with ovarian cancer.ObjectiveTo explore the correlation among fear of disease progression， metacognition and quality of life in patients with ovarian cancer， and to test the role of fear of disease progression in the relationship between metacognition and quality of life， so as to provide references for improving the quality of life in patients with ovarian cancer.MethodsA total of 135 patients with ovarian cancer hospitalized in Cangzhou People's Hospital of Hebei Province from January 2019 to December 2022 were selected. All subjects were requested to complete the Functional Assessment of Cancer Therapy-Ovarian Cancer （FACT-O）， Fear of Progression Questionnaire-Short Form （FoP-Q-SF） and Metacognition Questionnaire （MCQ） to assess their quality of life， fear of disease progression and metacognitive level. Pearson correlation analysis was adopted to examine the correlation among the above scales. Process v3.5 macro program was utilized to determine the mediating effect of fear of disease progression on the relationship between metacognition and quality of life， and nonparametric Bootstrap with bias-correction was used to test the mediating effect.ResultsA total of 122 patients （90.37%） with ovarian cancer completed the effective questionnaire survey. Patients scored （90.52±17.13） on FACT-O， （68.52±16.31） on MCQ， and （37.72±8.91） on FoP-Q-SF. Pearson correlation analysis denoted that FoP-Q-SF score was negatively correlated with FACT-O score （r=-0.412， P

Objective To explore changes of brain structure in patients with functional movement disorder (FMD) and their correlation with disease severity. Methods Nine patients with FMD (FMD group) as well as 10 gender⁃ and age⁃matched healthy volunteers (control group) were recruited in Ruijin Hospital, Shanghai Jiaotong University School of Medicine, from May to October 2023, and then they underwent MRI. Simplified Functional Movement Disorder Rating Scale (S ⁃FMDRS) was used to assess movement disorder symptoms and severity. Clinical Global Impression ⁃ Severity (CGI ⁃ S) was used to evaluate disease severity. Somatoform Dissociation Questionnaire ⁃ 20 (SDQ ⁃20) was used to assess body dissociation. Chinese version of Maltreatment and Abuse Chronology of Exposure (MACE) was used to assess childhood abuse situation. 36⁃Item Short Form of Health Survey (SF⁃36) was used to assess health status. Hamilton Anxiety Rating Scale (HAMA) and Hamilton Depression Rating Scale 17 (HAMD⁃17) were used to evaluate anxiety and depression. Results Compared with the control group, the FMD group exhibited higher scores on SDQ⁃20 (Z = 2.259, P = 0.028) and Chinese version of MACE (t = ⁃ 3.570, P = 0.002) as well as HAMA (t = ⁃ 3.999, P = 0.001), while SF⁃36 scores for vitality (t = 3.091, P = 0.009) and social functioning (t = 2.275, P = 0.046) were lower in the FMD group. Cortical thickness was greater in the FMD group in the right inferior occipital gyrus and sulcus cortex (t = ⁃ 2.924, P = 0.009), right anterior cingulate gyrus and sulcus cortex (t = ⁃ 3.286, P = 0.004), right orbitofrontal cortex (t = ⁃ 2.381, P = 0.029), while thickness in the left subcallosal area (t = 2.492, P = 0.023) and left paracentral lobule gyrus and sulcus (t = 2.139, P = 0.047), as well as volume of the right cerebellar white matter (t = 2.685, P = 0.016), left cerebellar white matter (t = 2.121, P = 0.049), and the middle posterior region of the corpus callosum (t = 2.550, P = 0.021) were smaller than those of the control group. Correlation analysis revealed left cerebellar white matter were positively correlated with disease duration (r = 0.837, P = 0.038), while cortical thickness in the left subcallosal area were negatively correlated with disease duration (r = ⁃ 0.831, P = 0.040). Conclusions There are structural changes in the limbic system, sensorimotor area, visual cortex, and certain white matter regions in the brains of FMD patients, and several regions were correlated with the disease duration.

In this article, we investigate the concepts of monotonicity, Schur-geometric convexity, Schur-harmonic convexity, and Schur-power convexity for the lower and upper limits of the integral mean, focusing on convex functions on coordinate axes. Furthermore, we introduce novel and fascinating inequalities for binary means as a practical application.

C22H26O6, orthorhombic, P212121 (no. 19), a = 7.6648(5) Å, b = 11.2663(6) Å, c = 22.7321(7) Å, V = 1963.0(2) Å3, Z = 4, Rgt (F) = 0.0652, wRref (F 2) = 0.1399, T = 273(2) K.

Ginseng and American ginseng need to be covered and protected against cold in winter to survive, and covering and uncovering date has great influences on safe overwintering and seedling emergence. To determine the optimal time, field experiments are conducted at Fusong of Jilin Province from November 2021 to June 2022. Effects of different covering dates on the soil temperature in the overwintering layer and seedling emergence rate of ginseng and American ginseng are studied through different experiment themes. For some cases they are covered in stages in early winter and simultaneously uncovered in spring, and there are also other cases when the ginseng and American ginseng are covered simultaneously in early winter and uncovered step by step in spring. Results show that the emergence rate of ginseng and American ginseng decreases with the delay of coverage date. When 5 cm soil temperature is 0℃, the coverage date for cold prevention is the best, with the highest emergence rate of ginseng and American ginseng. Below -12℃, most or all of American ginseng is frozen to death. When the instantaneous 5 cm soil temperature drops to -14℃, the emergence rate of ginseng still reaches 75%. The emergence rate of ginseng seedlings in other treatments above this temperature coverage is mostly over 86%. When the soil temperature is -7℃ or above, the emergence rate of ginseng seedlings is above 97%. The emergence rate of ginseng seedlings covered by snow is 91%. The soil temperature fluctuates between -14℃ and -8℃, and all uncovered ginseng with an extreme minimum of -16℃ is frozen to death. When ginseng seedlings emerge, the soil temperature of 5-20 cm is around 8-9℃, slightly higher than that of American ginseng. Covering with high insulation fibers to prevent cold, the later the cover is removed, the lower the soil temperature becomes, and the later the seedlings emerge. The date of uncovering affects the progress of seedling emergence, but has little correlation with the emergence rate. The optimal date of uncovering needs to be analyzed further. On frost prone areas in early spring, combined with climate prediction, the emergence progress can be adjusted by uncovering date to avoid spring freezing damage.

The metaverse has captured significant attention as it provides a virtual realm that cannot be experienced in the physical world. Programmable optical holograms, integral components of the metaverse, allow users to access diverse information without needing external equipment. Meta-devices composed of artificially customized nano-antennas are excellent candidates for programmable optical holograms due to their compact footprint and flexible electromagnetic manipulation. Programmable optical meta-holograms can dynamically alter reconstructed images in real-time by directly modulating the optical properties of the metasurface or by modifying the incident light. Information can be encoded across multiple channels and freely selected through switchable functionality. These advantages will broaden the range of virtual scenarios in the metaverse, facilitating further development and practical applications. This review concentrates on recent advancements in the fundamentals and applications of programmable optical meta-holograms. We aim to provide readers with general knowledge and potential inspiration for applying programmable optical meta-holograms, both intrinsic and external ways, into the metaverse for better performance. An outlook and perspective on the challenges and prospects in these rapidly growing research areas are provided.

Objective To explore mechanism of prohibitin 2(PHB2) inhibition on the apoptosis of non-small cell lung cancer cell line A549. Methods For stable knockdown of PHB2, A549 strain was infected with lentiviruses. A549 cells with PHB2 depletion were incubated with Mdivi-1(DRP1 inhibitor). Apoptotic index of A549 cells was evaluated by TUNEL and annexin V-FITC/PI. Mitochondrial morphology was visualized by MitoTracker. ATP content, citrate synthase activity and the level of cytochrome c in cytosolic and mitochondrial fractions were analyzed by commercially available kits. Western blot was used to measure the levels of PHB2, DRP1 and p-DRP1 protein expression. Results Compared with the shCtrl group, the apoptosis of A549 cells increased significantly (P＜0.05) with up-regulated DRP1-dependent mitochondrial fission (P＜0.05), down-regulated ATP content (P＜0.05) and citrate synthase activity (P＜0.05). The level of cytochrome C was decreased in mitochondrial fractions (P＜0.05). Compared with the shPHB2 group, the apoptosis of A549 cells decreased significantly (P＜0.05) with alleviated DRP1-dependent mitochondrial fission (P＜0.05), increase of ATP content (P＜0.05) and citrate synthase activity(P＜0.05). The level of cytochrome C was increased in mitochondrial fractions (P＜0.05) in the shPHB2+Mdivi-1 group. Conclusions PHB2 inhibition can significantly enhance the apoptosis rate of A549 cells, potentially through promoting DRP1-dependent mitochondrial fission.

ObjectiveTo explore the effects and satisfaction of reference-induced blended teaching method in medical statistics teaching for undergraduate students in clinical medicine.MethodA total of 83 clinical medical undergraduate students from the class of 2018 at the Tongji University School of Medicine were enrolled into the study. The effect evaluation of blended teaching based on reference-induced self education （RISE） in the course of medical statistics was conducted. A self-designed questionnaire was used to assess student satisfaction.ResultsCompared with the traditional teaching method group， students’ distinguished achievement rate and pass rate in total score from RISE blended teaching group were 53.7% and 96.3%， respectively， and 83.3% students were satisfied with the overall effect. The RISE blended teaching method had higher satisfaction in stimulating learning interest （90.7% satisfaction rate， P=0.034） and cultivating scientific research ability （96.3% satisfaction rate， P=0.048）.ConclusionThe RISE blended teaching method， which integrates literature guidance with online and offline approaches， organically combines statistical knowledge with clinical research problems. It yields positive results in medical statistics education， stimulating learning interest， and fostering innovative scientific research abilities.

In order to further strengthen the standardization and homogenization of nursing care for severe and critically ill patients and give full play to the advantages of integrated traditional Chinese and western medicine nursing, Beijing Administration of Traditional Chinese Medicine (TCM)commissioned the Beijing Office of TCM Nursing Capacity Improvement Project to organize the specialized group to compile Nursing Specification of Integrated Traditional Chinese and Western Medicine for severe and critical patients with COVID-19. This specification elaborates the nursing contents of integrated traditional Chinese and Western Medicine for severe and critical patients with COVID-19 in detail, including etiology and pathogenesis, severe/critical high-risk groups, clinical classification and nursing measures, etc. Among them, nursing measures include disease observation and nursing, medication nursing, respiratory support care, prone position treatment and nursing, nutritional support nursing, general nursing, convalescent nursing, etc. This specification is convenient for clinical nurses in various medical institutions to use as reference, and provides reference for clinical application of integrated Chinese and western medicine in nursing severe and critical patients infected with COVID-19. (为进一步加强临床重型、危重型患者护理的规范化和同质化, 发挥中西医结合护理的优势, 北京市中医管理局委托北京市中医护理能力提升工程办公室组织专家编写了《新型冠状病毒感染重型、危重型患者中西医结合护理规范》。此规范对新型冠状病毒感染重型、危重型患者中西医结合护理内容进行了详细的阐述, 包括病因病机、重型/危重型高危人群、临床分型、护理措施等内容, 其中护理措施包括病情观察与护理、用药护理、呼吸支持护理、俯卧位治疗护理、营养支持护理、一般护理、康复期护理等内容, 方便各医疗机构临床护理人员参照使用, 为临床应用中西医结合护理新型冠状病毒感染重型、危重型患者提供参考。)

Multi-carrier modulation technology is an advanced modulation method commonly used in broadband communication systems. However, multi-carrier signals will produce a very large Peak to Average Power Ratio (PAPR) in the time domain, which will lead to non-linear damage and seriously affects the performance of system. In this paper, a joint PAPR reduction technology enabled by neural network-based coding and companding is proposed. The frequency-domain coding is implemented by a fully connected layer, which greatly reduces the complexity and difficulty of network training. A small proportion of spread spectrum is introduced to provide coding gain, and the time-domain companding network uses a nonlinear convolutional neural network to reduce PAPR. The effect of the scheme is shown through simulation under various parameter conditions, and compared with various schemes. The simulation results show that the scheme can reduce PAPR by 5 dB while introducing rare distortion. Finally, the scheme is experimentally verified. The experimental results show that the Bit Error Ratio (BER) is reduced by 75% compared with the clipping scheme when the PAPR is reduced by 5 dB, which verifies the feasibility of the scheme.

ObjectiveTo understand the pollution status of cadmium in wheat in 16 cities of Shandong Province and evaluate its dietary exposure and health risks.MethodsInductively coupled plasma mass spectrometry was performed to detect the cadmium content in wheat in 16 cities in Shandong Province from 2020 to 2021， and the dietary exposure to cadmium was calculated based on the wheat consumption of Shandong residents. The health risks caused by cadmium were evaluated using the margin of safety（MOS） and the health risk assessment model recommended by the United States Environmental Protection Agency.ResultsThe median of cadmium in wheat was 0.020 2 mg/kg， and the detection rate was 97.5%. The cadmium pollution in wheat was higher in southern Shandong Province and Jiaodong Peninsula， and lower in northwestern Shandong Province. The average monthly intake of cadmium from wheat by Shandong residents was 1.80 µg/kg·BW， which was less than the reference value of PTMI（25 µg/kg·BW）. The MOS of cadmium in wheat was 13.9 and the risk was acceptable. The average annual personal cancer risk was 0.46 × 10-5 per year， which was less than the standard of the international commission on radiation protection.ConclusionThere are regional differences in wheat pollution in Shandong Province， and the health risk of residents ingesting cadmium through wheat is at an acceptable level.

Molds and yeasts are common fungi that cause food contamination， and are also used as indicators to evaluate the quality of food hygiene. China has been conducting contamination surveillance of molds and yeasts in food since 1984， and has greatly promoted the quality of food hygiene nationwide. However， the key technical aspects of Chinese standard GB 4789.15—2016 “National food safety standards of food microbiological examination - molds and yeasts enumeration” and foreign standards from other countries or international organizations are rather different. Therefore，to adapt to the needs of international trade and better promote Chinese standards in line with foreign or international standards， this article reviews the comparative analysis of foreign or international standards such as BAM Chapter 18—2001 from the US， AS 5013.29—2009 from Australia， and ISO 21527—2008 from the International Organization for Standardization， with the Chinese standard （GB 4789.15—2016） on mold and yeast enumeration to provide a theoretical basis and reference method for the subsequent revision of the Chinese standard on mold and yeast enumeration in food.

Abstract Background Attention deficit hyperactivity disorder (ADHD) is commonly associated with developmental dyslexia (DD), which are both prevalent and complicated pediatric neurodevelopmental disorders that have a significant influence on children’s learning and development. Clinically, the comorbidity incidence of DD and ADHD is between 25 and 48%. Children with DD and ADHD may have more severe cognitive deficiencies, a poorer level of schooling, and a higher risk of social and emotional management disorders. Furthermore, patients with this comorbidity are frequently treated for a single condition in clinical settings, and the therapeutic outcome is poor. The development of effective treatment approaches against these diseases is complicated by their comorbidity features. This is often a major problem in diagnosis and treatment. In this study, we developed bioinformatical methodology for the analysis of the comorbidity of these two diseases. As such, the search for candidate genes related to the comorbid conditions of ADHD and DD can help in elucidating the molecular mechanisms underlying the comorbid condition, and can also be useful for genotyping and identifying new drug targets. Results Using the ANDSystem tool, the reconstruction and analysis of gene networks associated with ADHD and dyslexia was carried out. The gene network of ADHD included 599 genes/proteins and 148,978 interactions, while that of dyslexia included 167 genes/proteins and 27,083 interactions. When the ANDSystem and GeneCards data were combined, a total of 213 genes/proteins for ADHD and dyslexia were found. An approach for ranking genes implicated in the comorbid condition of the two diseases was proposed. The approach is based on ten criteria for ranking genes by their importance, including relevance scores of association between disease and genes, standard methods of gene prioritization, as well as original criteria that take into account the characteristics of an associative gene network and the presence of known polymorphisms in the analyzed genes. Among the top 20 genes with the highest priority DRD2, DRD4, CNTNAP2 and GRIN2B are mentioned in the literature as directly linked with the comorbidity of ADHD and dyslexia. According to the proposed approach, the genes OPRM1, CHRNA4 and SNCA had the highest priority in the development of comorbidity of these two diseases. Additionally, it was revealed that the most relevant genes are involved in biological processes related to signal transduction, positive regulation of transcription from RNA polymerase II promoters, chemical synaptic transmission, response to drugs, ion transmembrane transport, nervous system development, cell adhesion, and neuron migration. Conclusions The application of methods of reconstruction and analysis of gene networks is a powerful tool for studying the molecular mechanisms of comorbid conditions. The method put forth to rank genes by their importance for the comorbid condition of ADHD and dyslexia was employed to predict genes that play key roles in the development of the comorbid condition. The results can be utilized to plan experiments for the identification of novel candidate genes and search for novel pharmacological targets.

BackgroundHeat pipe cooled reactor (HPR) has many characteristics, such as reliability, inherent safety, small volume, modularity, and solid core. The nuclear fuel of solid core is seriously affected by high temperature, strong irradiation, and solid constraint when operating, which affect the heat transfer performance and mechanical properties of the core seriously. The stress and gap heat transfer caused by the contact between monolith and other components change nonlinearly with the increase of burnup, and they influence each other. Therefore, the coupled irradiation-thermal-mechanical behavior of the monolith is a complex multi-physics phenomena.PurposeThis study aims to develop a coupled irradiation-thermal-mechanical model to explore the characteristics of gap variation, heat transfer and mechanics during the lifetime of solid core.MethodsFirst of all, based on the geometric parameter and material of a typical solid core of HPR with fuel rod composed of UO2 pellets and 316 stainless steel cladding, a coupled irradiation-thermal-mechanical model was developed and applied to the finite element multi-physics field analysis software COMSOL. The calculation parameter settings mainly referred to the design parameters of the MegaPower reactor. Then, a thermal conductivity model changing with the increase of burnup for UO2, the gap heat transfer model and mechanical contact were introduced in the gaps in the solid core, and both irradiation-induced deformation effect including densification and fission product swelling, and creep effect of UO2 pellets and 316 stainless steel monolith were taken into account. Finally, the model was applied to calculating the typical HPR and the characteristics of gap variation, heat transfer and mechanics were analyzed.ResultsAnalysis results show that pellet temperature and creep of monolith and cladding increase after complete contact between monolith and cladding. A smaller average number of heat pipes around the fuel rod result in higher temperature and stress distribution in the nearby area, and the cladding in this area has a risk of creep failure during its lifetime caused by internal pressure of the fuel rod and contact pressure between the monolith and cladding.ConclusionsThe gap contact can affect the heat transfer and mechanical properties of the solid core of HPR, and even result in an increase in the risk of cladding failure.

BackgroundThe enrichment of nuclear fuel may significantly influence the fuel performance of a reactor.PurposeThis study aims to explore the effect of U3Si2-Al plate-type fuel enrichment on its performance,MethodsThe fuel performance analysis code BEEs-Plates, neutronics Monte Carlo code OpenMC, and the one-dimensional system analysis code ZEBRA were coupled together within the MOOSE (Multiphysics Object-Oriented Simulation Environment) framework. Then the coupling code was employed to conduct the multiphysics coupling calculation for JRR-3 (Japanese Research Reactor No.3) fuel assembly enriched at 15%, 20%, and 25%. The data exchange among the three codes were realized with the help of interpolation transfer method implemented in MOOSE. Additionally, neutron physical parameters and fuel performance parameters after 231 d of operation were analyzed when the average fuel consumption of the module was 125.71 GWd∙tU-1.ResultsThe calculation results indicate that the max power density of fuel assembly enriched at 25% is 18% higher than the assembly enriched at 15%. Due to the high thermal conductivity of aluminum, the temperature difference in the fuel assembly is almost negligible whilst there is a significant difference in the fast neutron fluence. The results of fuel temperature and fast neutron fluence show that the volumetric strain is more obviously affected by the fuel temperature. Specifically, the plastic strain of the assembly with 25% enrichment is approximately 40% higher than that of the assembly with 15% enrichment.ConclusionThe analysis results of this study suggest that the assembly with a higher enrichment is more prone to failure.

[Objective] The spatiotemporal changes and correlations between landscape ecology risks and ecosystem service values in the Hanjiang eco-economic belt were studied in order to provide a theoretical basis for regional ecological security and sustainable development of the ecosystem and support for the optimization of landscape pattern and rational allocation of land resources. [Methods] We evaluated the ecological risk and ecosystem service value of landscape in the Hanjiang eco-economic belt based on an ecosystem service value equivalence table. We determined the internal relationship between these parameters, and the spatiotemporal variation law of landscape ecological risk and ecosystem service value. [Results] ① Landscape fragmentation gradually increased, and the landscape ecological risk slightly increased in the Hanjiang eco-economic belt from 1990 to 2020. A spatial pattern of “low risk in the upstream, high risk accompanied with low risk in the midstream, and high risk in the downstream” was observed. ② The ecosystem service value showed a slight increase from 1990 to 2020 of 8.66 billion yuan, with an annual growth rate of 0.06%. Regulation service accounted for 66.8% of the increase and support service accounted for 22.2%. A spatial pattern of “medium-high values in the upstream mountainous area, medium-high and low values in the middle reaches, and low values in the downstream” was observed. ③ Changes in ecosystem service value and ecological risk were negatively correlated. Low ecosystem service value was associated with high ecological risk, while high ecosystem service value was associated with low ecological risk. The significance of the correlations decreased before 2005 and increased after 2005. [Conclusion] Greater attention should be given to the system integrity of the Hanjiang eco-economic belt, and organic coordinated development within the basin should be strengthened. Additionally, greater attention should given to protecting biodiversity in the upstream region and to strengthening the control of ecological space use in the downstream region so as to comprehensively improve the ecosystem service value of the economic belt.

ObjectiveTo investigate the diagnostic value of trimethylamine N-Oxide （TMAO） and serum amyloid A （SAA） levels in peripheral blood of patients with chronic cerebral hypoperfusion （CCH）. MethodsThe patients attending the Department of Neurology of Sichuan Provincial Peoples Hospital from February 2022 to June 2022 were divided into CCH group （n=55） and control group （n=42） based on the results of magnetic resonance arterial spin labeling （ASL）. The levels of TMAO and SAA were detected by ELISA and their diagnostic value for CCH were anaylzed by ROC curve. ResultsThe levels of TMAO （47.24±8.84 vs 40.81±8.33, t=3.639, P＜0.05） and SAA （14.04±2.70 vs 12.20±2.30, t=3.542, P＜0.05） in CCH group were significantly higher than those in control group. High levels of TMAO and SAA and history of primary hypertension were independent risk factors for the occurrence of CCH （P＜0.05）. The areas under ROC curve （AUC） of TMAO and SAA for diagnosing CCH were 0.701 （95%CI: 0.594-0.809） respectively and 0.675 （95%CI: 0.565-0.786）. The combined detection of TMAO and SAA achieved the highest AUC of 0.765 （95%CI: 0.667-0.863）. ConclusionBoth TMAO and SAA are related to the occurrence of CCH and can be used as auxiliary diagnostic indicators for CCH.